Hymenobacter sp. Fur1
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4945 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A558C4H3|A0A558C4H3_9MICO N-acetyltransferase OS=Hymenobacter sp. Fur1 OX=2594794 GN=FNT36_05510 PE=4 SV=1
MM1 pKa = 7.9 PKK3 pKa = 9.48 PDD5 pKa = 4.54 RR6 pKa = 11.84 KK7 pKa = 9.68 PDD9 pKa = 3.64 PDD11 pKa = 4.25 FEE13 pKa = 6.1 PDD15 pKa = 3.05 YY16 pKa = 10.76 DD17 pKa = 4.2 TADD20 pKa = 3.34 VEE22 pKa = 4.5 YY23 pKa = 10.97 DD24 pKa = 3.6 DD25 pKa = 5.97 ALPTYY30 pKa = 8.21 YY31 pKa = 10.52 HH32 pKa = 6.65 EE33 pKa = 4.63 VADD36 pKa = 4.78 IEE38 pKa = 4.73 YY39 pKa = 7.31 EE40 pKa = 4.35 TPADD44 pKa = 3.97 PDD46 pKa = 3.72 YY47 pKa = 11.81 DD48 pKa = 5.95 DD49 pKa = 4.42 IAQGSDD55 pKa = 3.04 EE56 pKa = 5.62 AEE58 pKa = 4.22 LADD61 pKa = 4.18 PDD63 pKa = 4.27 YY64 pKa = 11.6 EE65 pKa = 4.33 EE66 pKa = 5.1 VPQVPALIEE75 pKa = 4.02 PEE77 pKa = 4.15 PVVMQAASTLFAFEE91 pKa = 3.82 VGRR94 pKa = 11.84 AVLPLTQALPHH105 pKa = 5.05 TVVWRR110 pKa = 11.84 GYY112 pKa = 10.1 LKK114 pKa = 10.42 EE115 pKa = 4.01 PHH117 pKa = 6.91 PVTGLLRR124 pKa = 11.84 RR125 pKa = 11.84 VPVYY129 pKa = 10.52 RR130 pKa = 11.84 LADD133 pKa = 4.46 GYY135 pKa = 8.93 WDD137 pKa = 3.9 CYY139 pKa = 10.58 RR140 pKa = 11.84 EE141 pKa = 4.14 EE142 pKa = 4.22 EE143 pKa = 4.36 LQVAA147 pKa = 4.46
Molecular weight: 16.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.923
IPC_protein 3.897
Toseland 3.694
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.037
Wikipedia 3.795
Rodwell 3.732
Grimsley 3.605
Solomon 3.872
Lehninger 3.821
Nozaki 3.986
DTASelect 4.19
Thurlkill 3.732
EMBOSS 3.808
Sillero 4.012
Patrickios 1.138
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.914
Protein with the highest isoelectric point:
>tr|A0A558BN74|A0A558BN74_9MICO tRNA (Adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB OS=Hymenobacter sp. Fur1 OX=2594794 GN=tsaB PE=4 SV=1
MM1 pKa = 7.45 LPCAAKK7 pKa = 9.03 WVSVFLLSTVKK18 pKa = 10.54 FLGGPLAGSSLGLGFWQTLGLTVAGMMTSVFVFSGIGRR56 pKa = 11.84 AWVRR60 pKa = 11.84 HH61 pKa = 4.8 RR62 pKa = 11.84 QQQRR66 pKa = 11.84 RR67 pKa = 11.84 LRR69 pKa = 11.84 RR70 pKa = 11.84 APVFSKK76 pKa = 10.55 RR77 pKa = 11.84 SRR79 pKa = 11.84 NIIKK83 pKa = 9.79 VFKK86 pKa = 9.62 KK87 pKa = 10.3 FGMGGIAFLTPILLSPIGGTVIATLLGVPRR117 pKa = 11.84 QRR119 pKa = 11.84 ILLHH123 pKa = 5.63 MFWSAVLWGGVLTLASSRR141 pKa = 11.84 ISHH144 pKa = 7.1 LLHH147 pKa = 6.7 HH148 pKa = 7.17
Molecular weight: 16.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.442
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.093
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4945
0
4945
1754580
25
3065
354.8
38.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.131 ± 0.053
0.693 ± 0.01
4.843 ± 0.022
4.988 ± 0.035
3.926 ± 0.023
7.748 ± 0.033
2.1 ± 0.018
4.128 ± 0.027
3.829 ± 0.034
11.283 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.707 ± 0.017
3.648 ± 0.032
5.44 ± 0.028
4.81 ± 0.025
5.951 ± 0.031
5.471 ± 0.031
6.378 ± 0.046
7.029 ± 0.027
1.3 ± 0.016
3.595 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here