Sideroxydans lithotrophicus (strain ES-1)
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2977 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
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Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D5CSF1|D5CSF1_SIDLE Glutamine-dependent NAD(+) synthetase OS=Sideroxydans lithotrophicus (strain ES-1) OX=580332 GN=nadE PE=3 SV=1
MM1 pKa = 7.84 CLTSLPIAGTVKK13 pKa = 8.83 ITSPFGMRR21 pKa = 11.84 SGTNHH26 pKa = 6.63 AGIDD30 pKa = 3.27 IRR32 pKa = 11.84 ASVGTGVFASGAGTVVRR49 pKa = 11.84 ASDD52 pKa = 3.43 NPGPHH57 pKa = 6.59 NYY59 pKa = 10.16 GNVVVIDD66 pKa = 3.37 HH67 pKa = 6.65 GIIEE71 pKa = 4.49 GEE73 pKa = 4.24 HH74 pKa = 6.26 VYY76 pKa = 10.94 SLYY79 pKa = 11.33 AHH81 pKa = 7.14 LSEE84 pKa = 5.07 FDD86 pKa = 3.18 SALAVGQVVAAGQPLGLSGGKK107 pKa = 9.72 KK108 pKa = 9.24 GAPGSGSSQDD118 pKa = 3.29 PHH120 pKa = 8.26 LHH122 pKa = 6.01 FEE124 pKa = 4.46 VIKK127 pKa = 10.92 SKK129 pKa = 9.63 TEE131 pKa = 3.96 IKK133 pKa = 10.15 WNTTGAMGYY142 pKa = 8.5 PGKK145 pKa = 10.37 VDD147 pKa = 4.75 RR148 pKa = 11.84 VNPWPEE154 pKa = 3.72 LFGTCDD160 pKa = 3.66 LPSPGMPLQLSTSLNATKK178 pKa = 10.41 QFNQRR183 pKa = 11.84 FGDD186 pKa = 4.08 PLTLDD191 pKa = 4.01 LNGDD195 pKa = 4.67 GINTVPLTNPPILFDD210 pKa = 3.57 HH211 pKa = 6.7 TGSGIKK217 pKa = 9.4 TGTGWIAPDD226 pKa = 4.36 DD227 pKa = 4.24 GFLVLDD233 pKa = 4.1 RR234 pKa = 11.84 NGNGTIDD241 pKa = 3.41 NGTEE245 pKa = 3.98 LFGDD249 pKa = 3.76 STPILDD255 pKa = 4.29 ANGNVIGKK263 pKa = 9.65 AKK265 pKa = 10.74 NGFDD269 pKa = 4.89 ALAQLDD275 pKa = 4.36 TNHH278 pKa = 7.07 DD279 pKa = 4.55 GIVDD283 pKa = 3.61 AQDD286 pKa = 3.99 ANFFDD291 pKa = 4.05 LQVWQDD297 pKa = 3.57 LNQDD301 pKa = 4.58 GISQANEE308 pKa = 3.39 LRR310 pKa = 11.84 YY311 pKa = 9.69 LADD314 pKa = 4.43 DD315 pKa = 4.89 LNITSINVAATQHH328 pKa = 5.2 SQMLASGNQMADD340 pKa = 3.09 LGSFTYY346 pKa = 11.06 ADD348 pKa = 3.94 GSTGATGSVSNMADD362 pKa = 2.81 INLALDD368 pKa = 3.57 TFHH371 pKa = 6.96 RR372 pKa = 11.84 TFVTPIPLTPAAEE385 pKa = 4.22 QLPDD389 pKa = 3.43 MQGSGKK395 pKa = 9.82 VRR397 pKa = 11.84 DD398 pKa = 3.65 LSEE401 pKa = 4.26 AVSLSSSLEE410 pKa = 3.86 AALTQYY416 pKa = 11.46 SNAGTRR422 pKa = 11.84 DD423 pKa = 3.32 EE424 pKa = 4.46 QYY426 pKa = 12.01 ALIDD430 pKa = 3.58 NLIAEE435 pKa = 4.62 WGKK438 pKa = 10.98 SSGFADD444 pKa = 3.62 MQTRR448 pKa = 11.84 AAANGYY454 pKa = 6.2 TLVYY458 pKa = 10.67 AGLTPTQQAHH468 pKa = 6.37 LSVLEE473 pKa = 3.9 QFTGSSYY480 pKa = 10.96 FRR482 pKa = 11.84 MPWEE486 pKa = 4.6 GNSGAMGASQGMTVCYY502 pKa = 10.26 DD503 pKa = 3.54 GNPKK507 pKa = 10.03 HH508 pKa = 6.81 IRR510 pKa = 11.84 IDD512 pKa = 3.58 LSPRR516 pKa = 11.84 FGQTRR521 pKa = 11.84 MLDD524 pKa = 3.21 DD525 pKa = 4.13 TYY527 pKa = 11.49 AILRR531 pKa = 11.84 DD532 pKa = 3.6 AVYY535 pKa = 10.22 QALLPQTRR543 pKa = 11.84 LLPYY547 pKa = 10.28 LDD549 pKa = 4.12 AVGLKK554 pKa = 10.08 QEE556 pKa = 5.28 DD557 pKa = 3.69 GDD559 pKa = 4.25 LVYY562 pKa = 11.2 DD563 pKa = 3.98 FVNVQEE569 pKa = 4.95 LFLAQILANPDD580 pKa = 3.12 KK581 pKa = 11.17 AIVDD585 pKa = 3.7 LVEE588 pKa = 4.34 FNRR591 pKa = 11.84 YY592 pKa = 8.57 GKK594 pKa = 10.15 DD595 pKa = 3.44 YY596 pKa = 10.59 FKK598 pKa = 11.32 SSDD601 pKa = 3.14 WEE603 pKa = 4.28 TQGWQLLGDD612 pKa = 4.05 TLNDD616 pKa = 3.07 ITLTNPTLTPAIQKK630 pKa = 8.31 TLADD634 pKa = 4.35 FNINMDD640 pKa = 3.53 GQPNQKK646 pKa = 10.57 ANAEE650 pKa = 4.07 VQIAKK655 pKa = 10.18 KK656 pKa = 10.54 DD657 pKa = 3.6 GSTLNGLWNGGVLVGNDD674 pKa = 3.68 GNDD677 pKa = 3.49 VLNGGYY683 pKa = 10.03 YY684 pKa = 10.66 GNNILLGGAGDD695 pKa = 4.11 DD696 pKa = 4.22 VITGGMLATNILQGGAGDD714 pKa = 4.53 DD715 pKa = 3.7 VLKK718 pKa = 11.02 GGWYY722 pKa = 10.32 NDD724 pKa = 3.57 TLQGGDD730 pKa = 3.93 GNDD733 pKa = 3.64 NLDD736 pKa = 4.06 GGWGNDD742 pKa = 3.56 VLDD745 pKa = 4.89 GGAGDD750 pKa = 5.88 DD751 pKa = 4.48 ILNGDD756 pKa = 4.04 TGNDD760 pKa = 3.27 VLTGGTGNDD769 pKa = 3.11 ILQGGSGNDD778 pKa = 2.95 TYY780 pKa = 11.65 VYY782 pKa = 11.02 NKK784 pKa = 10.52 GDD786 pKa = 3.55 GADD789 pKa = 3.56 IIIDD793 pKa = 3.16 NSYY796 pKa = 10.64 YY797 pKa = 9.39 HH798 pKa = 6.38 TANNILQLGAGITADD813 pKa = 3.98 TLFVTYY819 pKa = 10.46 DD820 pKa = 3.46 SVAVTVLLDD829 pKa = 3.39 MGNGDD834 pKa = 4.81 SIHH837 pKa = 6.7 IGSPTDD843 pKa = 3.6 LAIQTIQLADD853 pKa = 3.73 GSTLDD858 pKa = 3.12 VDD860 pKa = 3.62 TLLRR864 pKa = 11.84 QRR866 pKa = 11.84 SLVQNGTDD874 pKa = 3.91 GADD877 pKa = 2.96 ILQGSDD883 pKa = 2.67 SAYY886 pKa = 10.73 ADD888 pKa = 3.74 VLSGGAGDD896 pKa = 5.71 DD897 pKa = 3.8 ILNGGAGNDD906 pKa = 3.66 VLNGGTGNDD915 pKa = 3.6 VLNGGTGSDD924 pKa = 3.46 TYY926 pKa = 11.46 VYY928 pKa = 11.09 NLGDD932 pKa = 3.71 GSDD935 pKa = 4.43 RR936 pKa = 11.84 ITDD939 pKa = 3.42 SGRR942 pKa = 11.84 QYY944 pKa = 11.47 YY945 pKa = 10.13 DD946 pKa = 2.57 WQTRR950 pKa = 11.84 QYY952 pKa = 11.11 RR953 pKa = 11.84 NADD956 pKa = 3.64 TNVLSFGAGITADD969 pKa = 4.16 MLTVSYY975 pKa = 11.27 DD976 pKa = 3.71 SVTLAATLVLSNGDD990 pKa = 3.85 TIEE993 pKa = 4.91 IGAPDD998 pKa = 3.86 NLAIQTLQFADD1009 pKa = 4.17 GSTLDD1014 pKa = 3.42 VNTLVGQQTLTQVGTEE1030 pKa = 4.21 GNDD1033 pKa = 3.14 VLTGSDD1039 pKa = 3.45 SIMFRR1044 pKa = 11.84 DD1045 pKa = 4.48 SIQGLGGDD1053 pKa = 3.6 DD1054 pKa = 3.56 TLYY1057 pKa = 11.24 GGAGGDD1063 pKa = 3.94 TLDD1066 pKa = 4.6 GGAGNDD1072 pKa = 3.55 TLYY1075 pKa = 10.88 GQSGNDD1081 pKa = 3.19 ILIGGTGNDD1090 pKa = 3.71 TLSGGGGNDD1099 pKa = 3.0 TYY1101 pKa = 11.54 VYY1103 pKa = 11.02 NLGDD1107 pKa = 3.81 GADD1110 pKa = 3.89 TIIDD1114 pKa = 3.67 LVGTHH1119 pKa = 6.62 PEE1121 pKa = 4.35 GWGQWPTPDD1130 pKa = 4.18 INTLSFGSGITPDD1143 pKa = 3.17 MVKK1146 pKa = 9.91 IRR1148 pKa = 11.84 FVPDD1152 pKa = 3.44 PADD1155 pKa = 3.61 PTGSTGSIVFDD1166 pKa = 4.76 LGNGDD1171 pKa = 4.23 TINVGSGIQTSSNYY1185 pKa = 9.39 NLSVQTVQFADD1196 pKa = 4.09 GSSFTIDD1203 pKa = 3.09 QMLRR1207 pKa = 11.84 RR1208 pKa = 11.84 NKK1210 pKa = 10.09 FYY1212 pKa = 11.53 VEE1214 pKa = 4.06 GTATAEE1220 pKa = 4.02 SMTGFNHH1227 pKa = 7.1 FYY1229 pKa = 11.08 GNNLQGMGGDD1239 pKa = 3.7 DD1240 pKa = 3.75 TLTGTEE1246 pKa = 4.46 GNDD1249 pKa = 3.23 ILSGGAGNDD1258 pKa = 3.29 VLIGMGGSDD1267 pKa = 3.25 TYY1269 pKa = 10.85 IYY1271 pKa = 11.03 NFGDD1275 pKa = 3.77 GADD1278 pKa = 3.89 QIVDD1282 pKa = 4.01 YY1283 pKa = 10.52 PSGTQTASGWVAGTNTLSFGTGITADD1309 pKa = 3.79 MVTPRR1314 pKa = 11.84 YY1315 pKa = 9.59 DD1316 pKa = 3.16 RR1317 pKa = 11.84 SVNAIVLDD1325 pKa = 4.35 LGNGDD1330 pKa = 4.63 SINVGAADD1338 pKa = 4.35 ALTIQQLKK1346 pKa = 9.53 FADD1349 pKa = 4.23 GSTMNLNDD1357 pKa = 4.02 FLGQKK1362 pKa = 8.52 TLTEE1366 pKa = 4.31 VGTSGSDD1373 pKa = 3.18 VLYY1376 pKa = 10.75 GSNSSSYY1383 pKa = 11.25 SDD1385 pKa = 3.57 TLIGGAGNDD1394 pKa = 3.73 LLIGGRR1400 pKa = 11.84 GNDD1403 pKa = 2.95 TYY1405 pKa = 11.73 VFNLGDD1411 pKa = 3.77 GADD1414 pKa = 4.05 NIIDD1418 pKa = 3.72 TGVSDD1423 pKa = 3.77 MAHH1426 pKa = 5.87 GLGINVLSFGAGITADD1442 pKa = 4.13 MITVQLDD1449 pKa = 3.72 GEE1451 pKa = 4.52 FGDD1454 pKa = 3.8 VTLDD1458 pKa = 3.39 LGGGDD1463 pKa = 4.07 SVTIGQIDD1471 pKa = 5.24 LNTNDD1476 pKa = 4.48 LNGISIEE1483 pKa = 3.99 QLKK1486 pKa = 10.0 FADD1489 pKa = 4.68 GSTITPFEE1497 pKa = 4.24 LFAQKK1502 pKa = 10.86 GLDD1505 pKa = 3.71 VIGGDD1510 pKa = 3.65 MVDD1513 pKa = 3.82 VLQGAANWTNRR1524 pKa = 11.84 MQGGAGDD1531 pKa = 4.5 DD1532 pKa = 3.94 SLSGGSINDD1541 pKa = 3.5 ILDD1544 pKa = 3.92 GGDD1547 pKa = 3.51 GNDD1550 pKa = 3.64 WLDD1553 pKa = 3.57 GGLGSDD1559 pKa = 4.23 TLNGGAGNDD1568 pKa = 3.82 QLSGGYY1574 pKa = 10.59 GNDD1577 pKa = 3.14 VLTGGAGNDD1586 pKa = 3.52 TLFGGEE1592 pKa = 4.4 GSDD1595 pKa = 3.77 TYY1597 pKa = 11.1 IFNQGDD1603 pKa = 4.35 GVDD1606 pKa = 5.62 LIADD1610 pKa = 3.97 SNTAGQINTLRR1621 pKa = 11.84 LGAGLTVPMLRR1632 pKa = 11.84 LATTQDD1638 pKa = 3.33 NGLLTLDD1645 pKa = 4.87 FGNGDD1650 pKa = 3.54 AVVIGGFNRR1659 pKa = 11.84 NDD1661 pKa = 3.5 LLAGLSIQRR1670 pKa = 11.84 FEE1672 pKa = 4.46 FVDD1675 pKa = 4.13 GTVMTAQQLVDD1686 pKa = 4.1 LGINVDD1692 pKa = 3.68 GTTGDD1697 pKa = 3.92 DD1698 pKa = 3.56 VLLGSSSTDD1707 pKa = 3.08 YY1708 pKa = 11.1 MFGDD1712 pKa = 4.68 DD1713 pKa = 5.25 GNDD1716 pKa = 3.16 VLLAGAGDD1724 pKa = 3.91 DD1725 pKa = 4.66 ALDD1728 pKa = 3.76 GGYY1731 pKa = 11.12 GNDD1734 pKa = 3.34 VLSGEE1739 pKa = 4.44 GGNDD1743 pKa = 2.83 ILQGGIGYY1751 pKa = 9.72 DD1752 pKa = 3.37 VLYY1755 pKa = 10.86 GGTGNDD1761 pKa = 3.04 TYY1763 pKa = 11.6 VFNYY1767 pKa = 10.58 GDD1769 pKa = 3.8 GQDD1772 pKa = 5.04 RR1773 pKa = 11.84 IIDD1776 pKa = 3.79 NQGSNTLQFGAGIFASDD1793 pKa = 3.34 VTFSKK1798 pKa = 10.58 FGSDD1802 pKa = 3.65 LQIDD1806 pKa = 4.44 FSNGTDD1812 pKa = 3.64 RR1813 pKa = 11.84 IRR1815 pKa = 11.84 VVDD1818 pKa = 3.82 WFAGNSIEE1826 pKa = 4.67 TLTFNDD1832 pKa = 4.12 GTSLDD1837 pKa = 3.94 LRR1839 pKa = 11.84 AMEE1842 pKa = 4.85 PSFADD1847 pKa = 3.49 VPIVGTVADD1856 pKa = 5.52 DD1857 pKa = 3.4 ILTGSVGNDD1866 pKa = 3.12 TLAGGQGNDD1875 pKa = 3.41 TLIGGTGNDD1884 pKa = 3.08 TYY1886 pKa = 11.42 LFNLGDD1892 pKa = 4.2 GVDD1895 pKa = 3.73 QIYY1898 pKa = 10.49 EE1899 pKa = 4.03 LSGLGNPATDD1909 pKa = 3.23 NSIVFGAGITPDD1921 pKa = 3.15 MLSFNMEE1928 pKa = 3.92 VVQATDD1934 pKa = 2.46 AWRR1937 pKa = 11.84 TGYY1940 pKa = 11.14 GNTPSFPSTAADD1952 pKa = 3.41 MAPNEE1957 pKa = 4.26 TTRR1960 pKa = 11.84 QVMTISVGAQGDD1972 pKa = 4.69 AIQVMSGMNAIGKK1985 pKa = 9.09 FKK1987 pKa = 10.57 FADD1990 pKa = 3.94 GSEE1993 pKa = 4.18 FTLNEE1998 pKa = 4.73 LITWQDD2004 pKa = 2.8 AGLPTINDD2012 pKa = 4.37 TINDD2016 pKa = 3.53 PWRR2019 pKa = 11.84 ASTLDD2024 pKa = 4.02 GIGTSAVFNGGAGNDD2039 pKa = 3.35 IVIGGDD2045 pKa = 3.09 QYY2047 pKa = 12.12 DD2048 pKa = 3.8 SYY2050 pKa = 11.51 TFNLGDD2056 pKa = 4.08 GQDD2059 pKa = 3.76 VIADD2063 pKa = 3.97 LGGWNDD2069 pKa = 2.75 IRR2071 pKa = 11.84 FGAGITASDD2080 pKa = 3.6 ITWNYY2085 pKa = 10.4 DD2086 pKa = 3.31 PASATPFVLNVGPYY2100 pKa = 9.82 GDD2102 pKa = 5.11 SIAIANGEE2110 pKa = 3.81 QGIIQNFVFSDD2121 pKa = 3.82 GTAITFDD2128 pKa = 3.63 QLLAQQGGLPPVTPDD2143 pKa = 3.18 IPKK2146 pKa = 10.28 SLYY2149 pKa = 10.41 SYY2151 pKa = 11.43 NSGLLMGSNADD2162 pKa = 3.54 DD2163 pKa = 4.64 TINSDD2168 pKa = 3.29 VSASSGAAVVVGGKK2182 pKa = 10.31 GDD2184 pKa = 3.7 DD2185 pKa = 3.56 VMYY2188 pKa = 10.81 GPNSTFLFNVGDD2200 pKa = 4.13 GQDD2203 pKa = 3.61 TIQNKK2208 pKa = 9.0 GWGSSYY2214 pKa = 10.89 NDD2216 pKa = 2.91 SGMTLLFGTGITPDD2230 pKa = 2.93 ASFNIEE2236 pKa = 4.12 VIEE2239 pKa = 4.43 KK2240 pKa = 10.69 KK2241 pKa = 9.3 NTGQLWSRR2249 pKa = 11.84 QGQLGLDD2256 pKa = 3.72 SQDD2259 pKa = 2.81 ISIGYY2264 pKa = 8.77 GNQGDD2269 pKa = 3.73 QVFIQDD2275 pKa = 4.06 AYY2277 pKa = 10.02 TFQAGQNQVPGSLPSAWDD2295 pKa = 3.01 WWSGEE2300 pKa = 3.83 AQTIQSIRR2308 pKa = 11.84 KK2309 pKa = 8.18 IEE2311 pKa = 4.02 FANGVIWNYY2320 pKa = 9.65 EE2321 pKa = 4.23 DD2322 pKa = 3.47 IMAHH2326 pKa = 5.48 ATHH2329 pKa = 6.41 IVEE2332 pKa = 4.82 DD2333 pKa = 3.8 AASALVTGTGYY2344 pKa = 10.85 NDD2346 pKa = 4.95 RR2347 pKa = 11.84 IFAYY2351 pKa = 10.04 AGNSVLSGGLGNDD2364 pKa = 3.42 TYY2366 pKa = 11.46 VISEE2370 pKa = 4.26 SGDD2373 pKa = 3.38 YY2374 pKa = 10.64 TITDD2378 pKa = 3.56 SCGVGSGNNTVEE2390 pKa = 4.28 FAWNYY2395 pKa = 10.16 ADD2397 pKa = 5.65 SNFTLSNEE2405 pKa = 4.23 SGLTLNFDD2413 pKa = 3.29 NGATVRR2419 pKa = 11.84 LGGFDD2424 pKa = 4.19 PNDD2427 pKa = 3.77 PQASCSIDD2435 pKa = 3.23 SFKK2438 pKa = 11.04 FADD2441 pKa = 4.32 GTALTYY2447 pKa = 10.66 DD2448 pKa = 3.33 QLLTRR2453 pKa = 11.84 GIDD2456 pKa = 3.62 MQGSAASEE2464 pKa = 4.46 VIEE2467 pKa = 4.23 GTAVNDD2473 pKa = 5.4 RR2474 pKa = 11.84 IDD2476 pKa = 4.19 ALDD2479 pKa = 4.09 GNDD2482 pKa = 3.93 TIIGGKK2488 pKa = 10.39 GNDD2491 pKa = 3.15 ILRR2494 pKa = 11.84 GGEE2497 pKa = 4.02 GGDD2500 pKa = 3.34 TYY2502 pKa = 11.35 AYY2504 pKa = 10.62 DD2505 pKa = 4.66 LGDD2508 pKa = 3.92 GTDD2511 pKa = 3.74 TIIDD2515 pKa = 4.13 QGWHH2519 pKa = 4.43 WNGNVLVADD2528 pKa = 4.49 EE2529 pKa = 4.58 NTLQLGSGIDD2539 pKa = 3.46 QSNVSVSFNADD2550 pKa = 2.67 TGSIVLKK2557 pKa = 9.8 MADD2560 pKa = 3.96 GGSIDD2565 pKa = 4.53 LGQPGAFSVQKK2576 pKa = 10.66 VQFADD2581 pKa = 3.83 GTSWDD2586 pKa = 3.36 EE2587 pKa = 3.97 WGVIAHH2593 pKa = 6.84 LPGGNGNNLAPTQSGALLDD2612 pKa = 3.81 QDD2614 pKa = 3.91 ATQDD2618 pKa = 3.47 QAFTYY2623 pKa = 8.73 QIPDD2627 pKa = 3.41 SVFADD2632 pKa = 3.77 PNGDD2636 pKa = 3.35 RR2637 pKa = 11.84 LSYY2640 pKa = 10.16 SVAMADD2646 pKa = 4.12 GSAVPAWLQFDD2657 pKa = 4.64 PLTQTFTGTPEE2668 pKa = 4.0 NADD2671 pKa = 3.29 VRR2673 pKa = 11.84 SLNLSVTATDD2683 pKa = 3.64 AGGLSTSGTFILNVLNVNDD2702 pKa = 4.13 APVVSMALTDD2712 pKa = 3.5 QTVQEE2717 pKa = 4.38 SAEE2720 pKa = 4.08 FTFAVPDD2727 pKa = 3.98 GAFTDD2732 pKa = 3.14 IDD2734 pKa = 3.93 MNYY2737 pKa = 9.91 GDD2739 pKa = 4.46 SLIYY2743 pKa = 10.57 SATLTDD2749 pKa = 4.99 GSALPAWLTFDD2760 pKa = 3.86 AGAGVFSGVPAHH2772 pKa = 6.83 GDD2774 pKa = 3.36 VGVLDD2779 pKa = 4.1 VAVTATDD2786 pKa = 3.23 IDD2788 pKa = 4.27 GLTASTTFRR2797 pKa = 11.84 LDD2799 pKa = 3.0 IAGVAPSNQAPVANGDD2815 pKa = 4.03 TVTLDD2820 pKa = 3.43 QNSGQSIITASSLLANDD2837 pKa = 4.5 TDD2839 pKa = 4.46 PDD2841 pKa = 3.64 MGDD2844 pKa = 3.23 TLNIVSVDD2852 pKa = 3.17 ATSALGNSVMLGAAGDD2868 pKa = 3.89 VVFDD2872 pKa = 4.65 IGNKK2876 pKa = 8.91 YY2877 pKa = 9.54 QLLGAGQTAIDD2888 pKa = 3.74 TFNYY2892 pKa = 8.53 TVADD2896 pKa = 4.21 GSGATATATVTATITGVNDD2915 pKa = 3.73 APVTTADD2922 pKa = 3.33 VATAMQEE2929 pKa = 4.09 DD2930 pKa = 4.45 LAVTVMGNVLANDD2943 pKa = 3.62 TDD2945 pKa = 3.78 IDD2947 pKa = 3.8 QGTVLAVANAGTFAGQYY2964 pKa = 9.6 GQLALNADD2972 pKa = 3.71 GSYY2975 pKa = 10.25 TYY2977 pKa = 11.51 ALDD2980 pKa = 3.81 NASLGVQSLAEE2991 pKa = 4.1 GQVVTEE2997 pKa = 4.27 TFAYY3001 pKa = 9.88 QATDD3005 pKa = 3.44 GLVATPSTLTVTIIGTNDD3023 pKa = 2.9 APIATVDD3030 pKa = 3.48 TTTVQEE3036 pKa = 4.43 DD3037 pKa = 4.19 VSVTASGNVLSNDD3050 pKa = 3.11 ADD3052 pKa = 3.61 VDD3054 pKa = 3.69 QGTVLSVANVGVFIGQFGQLTLNADD3079 pKa = 3.51 GSYY3082 pKa = 10.21 TYY3084 pKa = 11.49 ALDD3087 pKa = 3.8 NNALAVQSLAEE3098 pKa = 4.15 GQVVTEE3104 pKa = 4.11 SFAYY3108 pKa = 9.43 QASDD3112 pKa = 4.48 GITSTPSTLTISITGTNDD3130 pKa = 3.07 APVVAADD3137 pKa = 3.8 TAAVQEE3143 pKa = 4.72 DD3144 pKa = 4.15 LSITAIGNVLANDD3157 pKa = 3.27 IDD3159 pKa = 3.97 IDD3161 pKa = 3.75 QGAVLSVANTGMFAGHH3177 pKa = 6.45 YY3178 pKa = 9.39 GQLNLNADD3186 pKa = 3.5 GSYY3189 pKa = 10.26 TYY3191 pKa = 10.65 TLDD3194 pKa = 3.58 NASLGVQSLAEE3205 pKa = 4.1 GQVVTEE3211 pKa = 4.27 TFAYY3215 pKa = 9.94 QATDD3219 pKa = 4.92 GITSTPSTLTVTITGTNDD3237 pKa = 3.05 APVTTVDD3244 pKa = 3.34 TAAVQEE3250 pKa = 5.12 DD3251 pKa = 3.85 ISIAASGNVLANDD3264 pKa = 3.61 SDD3266 pKa = 4.0 VDD3268 pKa = 3.48 QGTVLGVANAGEE3280 pKa = 4.22 FAGQYY3285 pKa = 9.9 GQLTLQADD3293 pKa = 4.36 GSYY3296 pKa = 10.33 TYY3298 pKa = 11.31 VLDD3301 pKa = 4.06 NASSAVQSLAAGQVITEE3318 pKa = 4.45 TFAYY3322 pKa = 9.82 QATDD3326 pKa = 3.59 GLVSTPSSLTVTITCTNDD3344 pKa = 2.87 APIVAVPLPDD3354 pKa = 3.7 SSTLEE3359 pKa = 3.75 DD3360 pKa = 3.57 QIFHH3364 pKa = 6.04 FQVPADD3370 pKa = 3.92 TFTDD3374 pKa = 3.24 IDD3376 pKa = 3.56 QGDD3379 pKa = 4.27 VLTYY3383 pKa = 9.84 HH3384 pKa = 6.84 ATMADD3389 pKa = 3.62 GSVLPDD3395 pKa = 3.4 WLTFDD3400 pKa = 3.48 ATTLTFSGVPSNWDD3414 pKa = 2.93 VGVFNVSVTATDD3426 pKa = 3.26 TGGLSATDD3434 pKa = 3.69 TFTLDD3439 pKa = 3.34 VQNVNDD3445 pKa = 4.09 APVVLNHH3452 pKa = 6.48 MADD3455 pKa = 3.18 QHH3457 pKa = 6.21 IAEE3460 pKa = 4.43 SHH3462 pKa = 6.61 CDD3464 pKa = 3.44 DD3465 pKa = 3.2 HH3466 pKa = 8.75 HH3467 pKa = 6.52 GFSFAVPANTFDD3479 pKa = 4.04 DD3480 pKa = 3.63 WDD3482 pKa = 3.86 IVHH3485 pKa = 7.15 GDD3487 pKa = 3.53 SLTYY3491 pKa = 9.84 SATMADD3497 pKa = 4.61 GEE3499 pKa = 5.39 KK3500 pKa = 10.41 LPCWLKK3506 pKa = 10.68 FDD3508 pKa = 4.92 AATCTFSGRR3517 pKa = 11.84 AEE3519 pKa = 4.57 DD3520 pKa = 3.77 SGNWDD3525 pKa = 3.07 ILLTVTDD3532 pKa = 3.83 RR3533 pKa = 11.84 AGASVSQVFNLSSGDD3548 pKa = 3.6 DD3549 pKa = 3.86 HH3550 pKa = 7.94 RR3551 pKa = 11.84 DD3552 pKa = 3.14 KK3553 pKa = 11.58 CHH3555 pKa = 7.48 DD3556 pKa = 4.08 DD3557 pKa = 3.27 QVLPIDD3563 pKa = 3.8 TTQDD3567 pKa = 3.41 EE3568 pKa = 5.5 IITSSSVNDD3577 pKa = 4.26 IIHH3580 pKa = 6.62 TGNGANTIVFMRR3592 pKa = 11.84 GYY3594 pKa = 10.34 GQDD3597 pKa = 3.12 KK3598 pKa = 10.09 VYY3600 pKa = 11.21 GSIGTDD3606 pKa = 3.02 NTVVLGGGIQMADD3619 pKa = 2.83 IALSKK3624 pKa = 10.52 QGNDD3628 pKa = 4.34 LILEE3632 pKa = 4.43 SGNNDD3637 pKa = 3.3 QITFKK3642 pKa = 10.65 NWYY3645 pKa = 6.27 DD3646 pKa = 3.44 TNVNHH3651 pKa = 7.44 KK3652 pKa = 10.44 SVLNLEE3658 pKa = 4.8 IISNAMSGFGEE3669 pKa = 4.52 DD3670 pKa = 3.3 NHH3672 pKa = 7.15 HH3673 pKa = 6.63 GKK3675 pKa = 9.58 HH3676 pKa = 6.78 DD3677 pKa = 3.65 DD3678 pKa = 3.62 HH3679 pKa = 9.24 LSIRR3683 pKa = 11.84 HH3684 pKa = 5.76 FDD3686 pKa = 3.35 FTAVVNAFDD3695 pKa = 4.56 QALTTNPALNAWSMTDD3711 pKa = 3.3 ALLSAHH3717 pKa = 7.12 LEE3719 pKa = 4.45 GCDD3722 pKa = 3.64 SSTLGGDD3729 pKa = 4.25 LANQFNQNGSLAAIALASSQTAINDD3754 pKa = 3.86 TNFGGIPQQLHH3765 pKa = 6.47 PFAGLQTTTAKK3776 pKa = 10.74 LGG3778 pKa = 3.51
Molecular weight: 392.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.723
IPC2_protein 3.528
IPC_protein 3.617
Toseland 3.363
ProMoST 3.808
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.605
Rodwell 3.439
Grimsley 3.261
Solomon 3.63
Lehninger 3.592
Nozaki 3.732
DTASelect 4.075
Thurlkill 3.427
EMBOSS 3.617
Sillero 3.745
Patrickios 1.748
IPC_peptide 3.63
IPC2_peptide 3.719
IPC2.peptide.svr19 3.694
Protein with the highest isoelectric point:
>tr|D5CQK7|D5CQK7_SIDLE Glutaredoxin 2 OS=Sideroxydans lithotrophicus (strain ES-1) OX=580332 GN=Slit_1005 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.14 QPSVTKK11 pKa = 10.56 RR12 pKa = 11.84 KK13 pKa = 7.91 RR14 pKa = 11.84 THH16 pKa = 5.88 GFLVRR21 pKa = 11.84 MKK23 pKa = 9.4 TKK25 pKa = 10.36 GGRR28 pKa = 11.84 AVIAARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 VRR41 pKa = 11.84 LGVV44 pKa = 3.29
Molecular weight: 5.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.411
IPC2_protein 11.082
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2977
0
2977
913564
30
3778
306.9
33.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.503 ± 0.054
1.01 ± 0.021
5.32 ± 0.046
5.897 ± 0.055
3.789 ± 0.028
7.565 ± 0.048
2.455 ± 0.029
5.498 ± 0.032
4.609 ± 0.042
10.624 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.778 ± 0.022
3.469 ± 0.037
4.409 ± 0.031
4.16 ± 0.035
5.836 ± 0.048
5.78 ± 0.035
5.152 ± 0.054
7.17 ± 0.037
1.264 ± 0.019
2.714 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here