Lactobacillus parafarraginis F0439
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3183 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G9ZRU6|G9ZRU6_9LACO Uncharacterized protein OS=Lactobacillus parafarraginis F0439 OX=797515 GN=HMPREF9103_02457 PE=4 SV=1
MM1 pKa = 8.18 DD2 pKa = 5.35 EE3 pKa = 4.3 LHH5 pKa = 7.03 IDD7 pKa = 3.53 QSPTEE12 pKa = 4.28 LTTVTNLINEE22 pKa = 3.98 ATEE25 pKa = 4.14 IVNHH29 pKa = 5.88 SVSSSEE35 pKa = 4.08 TQYY38 pKa = 9.34 QTSPIYY44 pKa = 10.68 DD45 pKa = 3.74 LAIKK49 pKa = 9.13 TLATQLYY56 pKa = 8.76 YY57 pKa = 10.76 DD58 pKa = 4.28 RR59 pKa = 11.84 EE60 pKa = 4.2 LSKK63 pKa = 11.05 GMSAGLLMMLDD74 pKa = 3.82 QLQGMVSGSDD84 pKa = 3.45 PDD86 pKa = 3.82 GTT88 pKa = 4.04
Molecular weight: 9.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.793
IPC2_protein 4.012
IPC_protein 3.91
Toseland 3.719
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.821
Rodwell 3.745
Grimsley 3.63
Solomon 3.872
Lehninger 3.834
Nozaki 4.024
DTASelect 4.202
Thurlkill 3.783
EMBOSS 3.834
Sillero 4.024
Patrickios 1.914
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.919
Protein with the highest isoelectric point:
>tr|G9ZLJ0|G9ZLJ0_9LACO ATP-dependent Clp protease ATP-binding subunit ClpX OS=Lactobacillus parafarraginis F0439 OX=797515 GN=clpX PE=3 SV=1
MM1 pKa = 8.12 RR2 pKa = 11.84 SRR4 pKa = 11.84 SLDD7 pKa = 3.48 FLITTTILEE16 pKa = 4.32 RR17 pKa = 11.84 GVTFPGIDD25 pKa = 3.64 VLVLKK30 pKa = 10.74 ADD32 pKa = 3.69 DD33 pKa = 5.09 RR34 pKa = 11.84 IFSSAALVQIAGRR47 pKa = 11.84 VGRR50 pKa = 11.84 NTEE53 pKa = 3.93 RR54 pKa = 11.84 PGGQVLLYY62 pKa = 10.19 CSTRR66 pKa = 11.84 SSSVKK71 pKa = 10.51 ACDD74 pKa = 3.58 RR75 pKa = 11.84 QIKK78 pKa = 9.92 QMNQKK83 pKa = 9.7 ARR85 pKa = 11.84 QLSS88 pKa = 3.63
Molecular weight: 9.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.472
IPC_protein 10.16
Toseland 10.716
ProMoST 10.379
Dawson 10.774
Bjellqvist 10.482
Wikipedia 10.979
Rodwell 10.965
Grimsley 10.804
Solomon 10.935
Lehninger 10.906
Nozaki 10.716
DTASelect 10.467
Thurlkill 10.701
EMBOSS 11.111
Sillero 10.716
Patrickios 10.76
IPC_peptide 10.935
IPC2_peptide 9.663
IPC2.peptide.svr19 8.637
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3183
0
3183
832771
34
2059
261.6
29.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.262 ± 0.085
0.478 ± 0.012
5.972 ± 0.052
4.6 ± 0.041
4.356 ± 0.034
6.83 ± 0.038
2.311 ± 0.023
7.111 ± 0.048
6.341 ± 0.043
9.305 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.697 ± 0.023
4.966 ± 0.036
3.867 ± 0.032
4.513 ± 0.038
4.139 ± 0.034
6.323 ± 0.055
6.296 ± 0.045
7.039 ± 0.032
1.052 ± 0.017
3.538 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here