Zamilon virus
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V6BPN4|V6BPN4_9VIRU Uncharacterized protein OS=Zamilon virus OX=1411887 GN=za3_9 PE=4 SV=1
MM1 pKa = 7.42 SHH3 pKa = 6.54 NCQNSNEE10 pKa = 4.38 PDD12 pKa = 3.23 TVYY15 pKa = 11.19 YY16 pKa = 10.49 DD17 pKa = 3.2 IVIPYY22 pKa = 9.26 NPNDD26 pKa = 3.92 SGLSPATFQAQLTQPLLYY44 pKa = 10.98 NPDD47 pKa = 3.41 KK48 pKa = 11.12 YY49 pKa = 10.92 YY50 pKa = 11.11 LSVVRR55 pKa = 11.84 FSIPTQYY62 pKa = 10.36 VPLMIPEE69 pKa = 4.44 IQPFPNTNVNNTIYY83 pKa = 10.79 SVTLEE88 pKa = 4.1 YY89 pKa = 11.23 NGVFSSQTFVQYY101 pKa = 10.7 DD102 pKa = 3.56 VSLTNPNDD110 pKa = 3.96 TPPPPPTINNRR121 pKa = 11.84 TVEE124 pKa = 4.2 PTAYY128 pKa = 9.41 YY129 pKa = 10.06 YY130 pKa = 11.07 VYY132 pKa = 10.58 NFSPFLQMINKK143 pKa = 9.18 ALSDD147 pKa = 3.94 AFTEE151 pKa = 3.77 ITMPVGAVAPYY162 pKa = 9.61 FVYY165 pKa = 10.68 SPVTQRR171 pKa = 11.84 ISLVAQRR178 pKa = 11.84 QFYY181 pKa = 10.47 DD182 pKa = 3.46 RR183 pKa = 11.84 NLAQPIRR190 pKa = 11.84 IYY192 pKa = 10.89 CNEE195 pKa = 3.82 ALFPFLDD202 pKa = 5.36 GIPFGGLDD210 pKa = 3.69 FNSVDD215 pKa = 4.1 GRR217 pKa = 11.84 DD218 pKa = 3.03 ILFNVEE224 pKa = 3.9 NLGNNLVQNQLTAPAYY240 pKa = 8.81 PPEE243 pKa = 5.07 FIQMEE248 pKa = 4.28 QEE250 pKa = 4.21 YY251 pKa = 8.58 ATLSNWNAIKK261 pKa = 9.78 TIQLVSNLLPINRR274 pKa = 11.84 EE275 pKa = 4.06 FIPSFRR281 pKa = 11.84 NTNVGVVNSQGILADD296 pKa = 4.07 FVPLVTLGPEE306 pKa = 3.77 SRR308 pKa = 11.84 TSIDD312 pKa = 3.43 YY313 pKa = 9.88 VANGPWRR320 pKa = 11.84 LIDD323 pKa = 3.48 MFGGVPITMVDD334 pKa = 3.81 LSVYY338 pKa = 8.26 WTDD341 pKa = 3.25 QIGRR345 pKa = 11.84 RR346 pKa = 11.84 FVLDD350 pKa = 3.37 VPRR353 pKa = 11.84 GRR355 pKa = 11.84 IATCKK360 pKa = 10.53 LIFIKK365 pKa = 10.4 KK366 pKa = 9.69 DD367 pKa = 3.48 LAGHH371 pKa = 5.1 TLSRR375 pKa = 11.84 KK376 pKa = 9.32
Molecular weight: 42.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.578
IPC2_protein 4.813
IPC_protein 4.724
Toseland 4.571
ProMoST 4.851
Dawson 4.698
Bjellqvist 4.838
Wikipedia 4.596
Rodwell 4.571
Grimsley 4.482
Solomon 4.698
Lehninger 4.647
Nozaki 4.8
DTASelect 5.016
Thurlkill 4.584
EMBOSS 4.609
Sillero 4.851
Patrickios 1.392
IPC_peptide 4.698
IPC2_peptide 4.838
IPC2.peptide.svr19 4.785
Protein with the highest isoelectric point:
>tr|V6BPP1|V6BPP1_9VIRU DNA packaging ATPase OS=Zamilon virus OX=1411887 GN=za3_4 PE=4 SV=1
MM1 pKa = 7.35 SKK3 pKa = 10.49 KK4 pKa = 10.49 VLLSGTAPRR13 pKa = 11.84 GGCFCPNCGTEE24 pKa = 3.98 VGGVLAGVLSGGRR37 pKa = 11.84 TVKK40 pKa = 10.4 RR41 pKa = 11.84 RR42 pKa = 11.84 VKK44 pKa = 10.5 KK45 pKa = 10.83 GGIGTKK51 pKa = 10.28 AGAKK55 pKa = 9.16 KK56 pKa = 10.45 SPWVAFLQKK65 pKa = 9.93 FAKK68 pKa = 8.6 EE69 pKa = 3.84 HH70 pKa = 4.73 GMKK73 pKa = 10.37 YY74 pKa = 10.19 GEE76 pKa = 4.55 AMQSPKK82 pKa = 10.82 AKK84 pKa = 9.88 KK85 pKa = 9.9 QYY87 pKa = 9.72 EE88 pKa = 4.06 ALKK91 pKa = 10.03 KK92 pKa = 10.65 KK93 pKa = 10.3 SGKK96 pKa = 4.66 TTKK99 pKa = 10.16 KK100 pKa = 9.94 KK101 pKa = 10.77 SSGSKK106 pKa = 7.97 TKK108 pKa = 10.51 KK109 pKa = 7.76 ITKK112 pKa = 9.42 KK113 pKa = 10.65 KK114 pKa = 9.34 PVKK117 pKa = 10.09 KK118 pKa = 10.03 SLKK121 pKa = 9.94 GGSKK125 pKa = 8.62 RR126 pKa = 11.84 TKK128 pKa = 10.07 RR129 pKa = 11.84 SPKK132 pKa = 9.47 MSNNDD137 pKa = 3.13 QRR139 pKa = 11.84 ILEE142 pKa = 4.3 EE143 pKa = 4.17 LMNMM147 pKa = 4.51
Molecular weight: 15.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.172
IPC2_protein 9.882
IPC_protein 9.897
Toseland 11.052
ProMoST 10.482
Dawson 11.096
Bjellqvist 10.657
Wikipedia 11.184
Rodwell 11.813
Grimsley 11.111
Solomon 11.14
Lehninger 11.14
Nozaki 11.008
DTASelect 10.657
Thurlkill 11.008
EMBOSS 11.418
Sillero 11.008
Patrickios 11.535
IPC_peptide 11.155
IPC2_peptide 9.165
IPC2.peptide.svr19 8.629
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
20
0
20
4928
73
778
246.4
28.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.485 ± 0.379
1.157 ± 0.221
5.641 ± 0.482
6.088 ± 0.764
4.282 ± 0.403
6.189 ± 1.109
1.238 ± 0.151
7.325 ± 0.476
10.329 ± 1.358
8.34 ± 0.348
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.597 ± 0.197
7.711 ± 0.464
4.282 ± 0.498
3.389 ± 0.428
3.369 ± 0.358
6.757 ± 0.588
5.662 ± 0.535
5.641 ± 0.394
0.609 ± 0.089
4.911 ± 0.403
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here