Zamilon virus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Maveriviricetes; Priklausovirales; Lavidaviridae; Sputnikvirus; Mimivirus-dependent virus Zamilon

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|V6BPN4|V6BPN4_9VIRU Uncharacterized protein OS=Zamilon virus OX=1411887 GN=za3_9 PE=4 SV=1
MM1 pKa = 7.42SHH3 pKa = 6.54NCQNSNEE10 pKa = 4.38PDD12 pKa = 3.23TVYY15 pKa = 11.19YY16 pKa = 10.49DD17 pKa = 3.2IVIPYY22 pKa = 9.26NPNDD26 pKa = 3.92SGLSPATFQAQLTQPLLYY44 pKa = 10.98NPDD47 pKa = 3.41KK48 pKa = 11.12YY49 pKa = 10.92YY50 pKa = 11.11LSVVRR55 pKa = 11.84FSIPTQYY62 pKa = 10.36VPLMIPEE69 pKa = 4.44IQPFPNTNVNNTIYY83 pKa = 10.79SVTLEE88 pKa = 4.1YY89 pKa = 11.23NGVFSSQTFVQYY101 pKa = 10.7DD102 pKa = 3.56VSLTNPNDD110 pKa = 3.96TPPPPPTINNRR121 pKa = 11.84TVEE124 pKa = 4.2PTAYY128 pKa = 9.41YY129 pKa = 10.06YY130 pKa = 11.07VYY132 pKa = 10.58NFSPFLQMINKK143 pKa = 9.18ALSDD147 pKa = 3.94AFTEE151 pKa = 3.77ITMPVGAVAPYY162 pKa = 9.61FVYY165 pKa = 10.68SPVTQRR171 pKa = 11.84ISLVAQRR178 pKa = 11.84QFYY181 pKa = 10.47DD182 pKa = 3.46RR183 pKa = 11.84NLAQPIRR190 pKa = 11.84IYY192 pKa = 10.89CNEE195 pKa = 3.82ALFPFLDD202 pKa = 5.36GIPFGGLDD210 pKa = 3.69FNSVDD215 pKa = 4.1GRR217 pKa = 11.84DD218 pKa = 3.03ILFNVEE224 pKa = 3.9NLGNNLVQNQLTAPAYY240 pKa = 8.81PPEE243 pKa = 5.07FIQMEE248 pKa = 4.28QEE250 pKa = 4.21YY251 pKa = 8.58ATLSNWNAIKK261 pKa = 9.78TIQLVSNLLPINRR274 pKa = 11.84EE275 pKa = 4.06FIPSFRR281 pKa = 11.84NTNVGVVNSQGILADD296 pKa = 4.07FVPLVTLGPEE306 pKa = 3.77SRR308 pKa = 11.84TSIDD312 pKa = 3.43YY313 pKa = 9.88VANGPWRR320 pKa = 11.84LIDD323 pKa = 3.48MFGGVPITMVDD334 pKa = 3.81LSVYY338 pKa = 8.26WTDD341 pKa = 3.25QIGRR345 pKa = 11.84RR346 pKa = 11.84FVLDD350 pKa = 3.37VPRR353 pKa = 11.84GRR355 pKa = 11.84IATCKK360 pKa = 10.53LIFIKK365 pKa = 10.4KK366 pKa = 9.69DD367 pKa = 3.48LAGHH371 pKa = 5.1TLSRR375 pKa = 11.84KK376 pKa = 9.32

Molecular weight:
42.5 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|V6BPP1|V6BPP1_9VIRU DNA packaging ATPase OS=Zamilon virus OX=1411887 GN=za3_4 PE=4 SV=1
MM1 pKa = 7.35SKK3 pKa = 10.49KK4 pKa = 10.49VLLSGTAPRR13 pKa = 11.84GGCFCPNCGTEE24 pKa = 3.98VGGVLAGVLSGGRR37 pKa = 11.84TVKK40 pKa = 10.4RR41 pKa = 11.84RR42 pKa = 11.84VKK44 pKa = 10.5KK45 pKa = 10.83GGIGTKK51 pKa = 10.28AGAKK55 pKa = 9.16KK56 pKa = 10.45SPWVAFLQKK65 pKa = 9.93FAKK68 pKa = 8.6EE69 pKa = 3.84HH70 pKa = 4.73GMKK73 pKa = 10.37YY74 pKa = 10.19GEE76 pKa = 4.55AMQSPKK82 pKa = 10.82AKK84 pKa = 9.88KK85 pKa = 9.9QYY87 pKa = 9.72EE88 pKa = 4.06ALKK91 pKa = 10.03KK92 pKa = 10.65KK93 pKa = 10.3SGKK96 pKa = 4.66TTKK99 pKa = 10.16KK100 pKa = 9.94KK101 pKa = 10.77SSGSKK106 pKa = 7.97TKK108 pKa = 10.51KK109 pKa = 7.76ITKK112 pKa = 9.42KK113 pKa = 10.65KK114 pKa = 9.34PVKK117 pKa = 10.09KK118 pKa = 10.03SLKK121 pKa = 9.94GGSKK125 pKa = 8.62RR126 pKa = 11.84TKK128 pKa = 10.07RR129 pKa = 11.84SPKK132 pKa = 9.47MSNNDD137 pKa = 3.13QRR139 pKa = 11.84ILEE142 pKa = 4.3EE143 pKa = 4.17LMNMM147 pKa = 4.51

Molecular weight:
15.89 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20

0

20

4928

73

778

246.4

28.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.485 ± 0.379

1.157 ± 0.221

5.641 ± 0.482

6.088 ± 0.764

4.282 ± 0.403

6.189 ± 1.109

1.238 ± 0.151

7.325 ± 0.476

10.329 ± 1.358

8.34 ± 0.348

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.597 ± 0.197

7.711 ± 0.464

4.282 ± 0.498

3.389 ± 0.428

3.369 ± 0.358

6.757 ± 0.588

5.662 ± 0.535

5.641 ± 0.394

0.609 ± 0.089

4.911 ± 0.403

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski