Sphingopyxis sp. LC363

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingopyxis; unclassified Sphingopyxis

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3880 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A095D7A4|A0A095D7A4_9SPHN Uncharacterized protein OS=Sphingopyxis sp. LC363 OX=1120705 GN=FG95_02616 PE=4 SV=1
MM1 pKa = 7.23LVWLAFTLAANPAIAQTTTTYY22 pKa = 11.49SNTTAGTINAATTCTALLVRR42 pKa = 11.84NFTVGASYY50 pKa = 9.78TVGDD54 pKa = 3.79VDD56 pKa = 5.31LGLLATHH63 pKa = 6.33SWRR66 pKa = 11.84GDD68 pKa = 3.32LRR70 pKa = 11.84VTLQSPAGTRR80 pKa = 11.84VQLVNGDD87 pKa = 3.53TGSIDD92 pKa = 3.27GNNFNVRR99 pKa = 11.84LDD101 pKa = 3.83DD102 pKa = 5.46DD103 pKa = 4.16AAQTVNTDD111 pKa = 3.29GNGTNHH117 pKa = 6.23SNSAPPYY124 pKa = 9.23QNVFSPNAPLSAFNGEE140 pKa = 3.99NSAGTWRR147 pKa = 11.84LEE149 pKa = 3.77ICDD152 pKa = 4.25QYY154 pKa = 11.7PSADD158 pKa = 3.04NGNFVRR164 pKa = 11.84ADD166 pKa = 3.86LYY168 pKa = 10.58LTGVPSSYY176 pKa = 11.58ADD178 pKa = 3.57LSLTKK183 pKa = 10.13SVSNAAPAFGASIDD197 pKa = 3.95YY198 pKa = 10.28VLSVTNASGSTLTATGVTVQDD219 pKa = 3.77NLPAGFNFTGASGFGSYY236 pKa = 10.47NSATGVWTVGSVPAGTTRR254 pKa = 11.84TLTISGTVAATAGASVTNSAEE275 pKa = 3.9IGASSAFDD283 pKa = 3.64FDD285 pKa = 4.05STPGNGAAGEE295 pKa = 4.5DD296 pKa = 4.26DD297 pKa = 3.9SDD299 pKa = 3.97SASFTVSGTRR309 pKa = 11.84VAGTPPALVCPVGTTVHH326 pKa = 6.84DD327 pKa = 4.06WDD329 pKa = 4.28SVTWPAGTTSAGAALTAIGSASFNIAVSGGSFLSNATYY367 pKa = 10.54GGQSPTRR374 pKa = 11.84QNVVTGGLTPAQYY387 pKa = 10.9SIFEE391 pKa = 4.42LVDD394 pKa = 3.73FTSQAGSVTTTITLPTAVPGAQFRR418 pKa = 11.84LFDD421 pKa = 3.32VDD423 pKa = 3.78YY424 pKa = 11.37ASGQFADD431 pKa = 3.84RR432 pKa = 11.84VTVTGTYY439 pKa = 10.31NGVAVTPILTNGTANYY455 pKa = 10.26VIGNSAYY462 pKa = 10.59GDD464 pKa = 3.68VLSADD469 pKa = 3.66GSADD473 pKa = 3.2GTVTVTFNAPVDD485 pKa = 4.66GITIEE490 pKa = 4.27YY491 pKa = 9.46GSHH494 pKa = 5.95SLAPANPGQQGMALHH509 pKa = 7.36DD510 pKa = 4.74IIFCRR515 pKa = 11.84PTANLVIAKK524 pKa = 7.96TSSVVSDD531 pKa = 4.03SVNGTANAKK540 pKa = 9.75AIPGARR546 pKa = 11.84MRR548 pKa = 11.84YY549 pKa = 9.37CILVTNNGSGTATGIAVADD568 pKa = 4.11ALPAGMSFIAGSLRR582 pKa = 11.84SGTSCAGATSVEE594 pKa = 4.07DD595 pKa = 4.47DD596 pKa = 3.78NANGADD602 pKa = 3.53EE603 pKa = 4.88SDD605 pKa = 3.49PFGASVAGTTVAATTATLLPAAAMAIAFEE634 pKa = 4.29VAIDD638 pKa = 3.46

Molecular weight: 64.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A095B5L1|A0A095B5L1_9SPHN Uncharacterized protein OS=Sphingopyxis sp. LC363 OX=1120705 GN=FG95_01358 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.21RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.57GFRR19 pKa = 11.84ARR21 pKa = 11.84KK22 pKa = 7.81ATVGGRR28 pKa = 11.84KK29 pKa = 9.05VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84AKK41 pKa = 10.7LSAA44 pKa = 4.0

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3880

0

3880

1254710

29

4404

323.4

34.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.601 ± 0.065

0.742 ± 0.011

6.227 ± 0.03

5.427 ± 0.033

3.635 ± 0.024

9.029 ± 0.046

1.932 ± 0.02

5.092 ± 0.025

3.153 ± 0.032

9.688 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.404 ± 0.021

2.578 ± 0.029

5.324 ± 0.03

2.895 ± 0.02

7.23 ± 0.042

5.167 ± 0.028

5.152 ± 0.035

6.973 ± 0.028

1.49 ± 0.017

2.261 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski