Bacillus gobiensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4231 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M4FLD6|A0A0M4FLD6_9BACI UDP-glucose 4-epimerase OS=Bacillus gobiensis OX=1441095 GN=AM592_15400 PE=3 SV=1
MM1 pKa = 7.37FFLVLLFASIVPLWTATAAAGEE23 pKa = 4.03WDD25 pKa = 4.16LLGGDD30 pKa = 3.99RR31 pKa = 11.84FSDD34 pKa = 4.27EE35 pKa = 3.97SDD37 pKa = 2.49IWPSGGGDD45 pKa = 3.47YY46 pKa = 10.58KK47 pKa = 10.72ICLSSEE53 pKa = 4.33SKK55 pKa = 10.15PGYY58 pKa = 8.49YY59 pKa = 9.75QLFEE63 pKa = 4.56EE64 pKa = 5.46DD65 pKa = 4.93PYY67 pKa = 12.02NPDD70 pKa = 3.5DD71 pKa = 3.73QVKK74 pKa = 8.67TGVYY78 pKa = 10.1FKK80 pKa = 10.28TAGSNDD86 pKa = 3.3FDD88 pKa = 5.72SNGCHH93 pKa = 5.76VYY95 pKa = 10.79KK96 pKa = 10.76DD97 pKa = 2.85IGGFVDD103 pKa = 4.11GTDD106 pKa = 3.87DD107 pKa = 3.95QAEE110 pKa = 4.45FYY112 pKa = 10.38LKK114 pKa = 10.04KK115 pKa = 10.34YY116 pKa = 10.83SSGEE120 pKa = 4.06SFAWFYY126 pKa = 11.56DD127 pKa = 3.39

Molecular weight: 14.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M3RAL8|A0A0M3RAL8_9BACI Uncharacterized protein OS=Bacillus gobiensis OX=1441095 GN=AM592_19075 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.27RR12 pKa = 11.84SKK14 pKa = 9.34VHH16 pKa = 5.82GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSKK25 pKa = 10.58NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4231

0

4231

1209575

26

4064

285.9

32.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.02 ± 0.04

0.74 ± 0.012

5.04 ± 0.031

7.457 ± 0.045

4.668 ± 0.031

6.84 ± 0.033

2.131 ± 0.02

7.865 ± 0.042

7.045 ± 0.04

9.903 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.645 ± 0.018

4.408 ± 0.026

3.659 ± 0.02

3.646 ± 0.025

4.015 ± 0.028

6.415 ± 0.028

5.268 ± 0.021

6.711 ± 0.028

1.05 ± 0.015

3.475 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski