Amino acid dipepetide frequency for Bacillus gobiensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.283AlaAla: 6.283 ± 0.087	0.636AlaCys: 0.636 ± 0.024	3.476AlaAsp: 3.476 ± 0.057	5.007AlaGlu: 5.007 ± 0.071	3.417AlaPhe: 3.417 ± 0.063	5.457AlaGly: 5.457 ± 0.075	1.265AlaHis: 1.265 ± 0.035	5.807AlaIle: 5.807 ± 0.074	4.654AlaLys: 4.654 ± 0.069	7.156AlaLeu: 7.156 ± 0.098	1.917AlaMet: 1.917 ± 0.049	2.786AlaAsn: 2.786 ± 0.052	2.106AlaPro: 2.106 ± 0.044	1.904AlaGln: 1.904 ± 0.047	2.582AlaArg: 2.582 ± 0.049	4.238AlaSer: 4.238 ± 0.065	3.026AlaThr: 3.026 ± 0.048	5.528AlaVal: 5.528 ± 0.071	0.618AlaTrp: 0.618 ± 0.022	2.334AlaTyr: 2.334 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.48CysAla: 0.48 ± 0.021	0.098CysCys: 0.098 ± 0.01	0.37CysAsp: 0.37 ± 0.021	0.462CysGlu: 0.462 ± 0.02	0.387CysPhe: 0.387 ± 0.019	0.642CysGly: 0.642 ± 0.025	0.181CysHis: 0.181 ± 0.012	0.562CysIle: 0.562 ± 0.024	0.37CysLys: 0.37 ± 0.018	0.786CysLeu: 0.786 ± 0.024	0.194CysMet: 0.194 ± 0.013	0.289CysAsn: 0.289 ± 0.015	0.353CysPro: 0.353 ± 0.019	0.24CysGln: 0.24 ± 0.015	0.316CysArg: 0.316 ± 0.017	0.48CysSer: 0.48 ± 0.02	0.425CysThr: 0.425 ± 0.019	0.427CysVal: 0.427 ± 0.018	0.07CysTrp: 0.07 ± 0.008	0.268CysTyr: 0.268 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.132AspAla: 3.132 ± 0.047	0.388AspCys: 0.388 ± 0.018	2.412AspAsp: 2.412 ± 0.051	4.23AspGlu: 4.23 ± 0.064	2.424AspPhe: 2.424 ± 0.047	3.306AspGly: 3.306 ± 0.06	1.288AspHis: 1.288 ± 0.033	3.989AspIle: 3.989 ± 0.062	3.147AspLys: 3.147 ± 0.057	5.014AspLeu: 5.014 ± 0.066	1.201AspMet: 1.201 ± 0.03	1.741AspAsn: 1.741 ± 0.043	2.164AspPro: 2.164 ± 0.049	2.148AspGln: 2.148 ± 0.04	2.32AspArg: 2.32 ± 0.048	2.897AspSer: 2.897 ± 0.044	2.407AspThr: 2.407 ± 0.042	3.468AspVal: 3.468 ± 0.059	0.639AspTrp: 0.639 ± 0.026	2.082AspTyr: 2.082 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	5.595GluAla: 5.595 ± 0.079	0.416GluCys: 0.416 ± 0.021	3.556GluAsp: 3.556 ± 0.064	6.826GluGlu: 6.826 ± 0.101	2.61GluPhe: 2.61 ± 0.057	4.324GluGly: 4.324 ± 0.062	1.534GluHis: 1.534 ± 0.041	5.609GluIle: 5.609 ± 0.072	6.828GluLys: 6.828 ± 0.084	7.279GluLeu: 7.279 ± 0.095	2.074GluMet: 2.074 ± 0.043	3.919GluAsn: 3.919 ± 0.065	2.088GluPro: 2.088 ± 0.043	3.203GluGln: 3.203 ± 0.053	3.379GluArg: 3.379 ± 0.059	3.842GluSer: 3.842 ± 0.051	3.849GluThr: 3.849 ± 0.058	4.511GluVal: 4.511 ± 0.066	0.909GluTrp: 0.909 ± 0.027	2.214GluTyr: 2.214 ± 0.043	0.0GluXaa: 0.0 ± 0.0
Phe	3.167PheAla: 3.167 ± 0.057	0.365PheCys: 0.365 ± 0.02	2.413PheAsp: 2.413 ± 0.049	2.836PheGlu: 2.836 ± 0.051	2.531PhePhe: 2.531 ± 0.063	3.382PheGly: 3.382 ± 0.066	1.119PheHis: 1.119 ± 0.028	3.919PheIle: 3.919 ± 0.072	2.364PheLys: 2.364 ± 0.047	4.858PheLeu: 4.858 ± 0.082	1.125PheMet: 1.125 ± 0.033	1.768PheAsn: 1.768 ± 0.04	1.734PhePro: 1.734 ± 0.041	1.791PheGln: 1.791 ± 0.044	1.667PheArg: 1.667 ± 0.038	3.556PheSer: 3.556 ± 0.064	2.645PheThr: 2.645 ± 0.055	3.181PheVal: 3.181 ± 0.054	0.479PheTrp: 0.479 ± 0.022	1.778PheTyr: 1.778 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	4.739GlyAla: 4.739 ± 0.078	0.637GlyCys: 0.637 ± 0.023	3.158GlyAsp: 3.158 ± 0.059	4.379GlyGlu: 4.379 ± 0.062	3.405GlyPhe: 3.405 ± 0.059	4.765GlyGly: 4.765 ± 0.082	1.377GlyHis: 1.377 ± 0.037	6.171GlyIle: 6.171 ± 0.088	5.055GlyLys: 5.055 ± 0.069	6.532GlyLeu: 6.532 ± 0.1	2.111GlyMet: 2.111 ± 0.05	2.792GlyAsn: 2.792 ± 0.048	1.792GlyPro: 1.792 ± 0.04	2.224GlyGln: 2.224 ± 0.049	2.604GlyArg: 2.604 ± 0.048	4.242GlySer: 4.242 ± 0.065	4.006GlyThr: 4.006 ± 0.068	4.826GlyVal: 4.826 ± 0.072	0.845GlyTrp: 0.845 ± 0.031	2.743GlyTyr: 2.743 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	1.329HisAla: 1.329 ± 0.039	0.203HisCys: 0.203 ± 0.012	0.983HisAsp: 0.983 ± 0.029	1.465HisGlu: 1.465 ± 0.035	1.12HisPhe: 1.12 ± 0.031	1.389HisGly: 1.389 ± 0.034	0.634HisHis: 0.634 ± 0.024	1.563HisIle: 1.563 ± 0.035	1.112HisLys: 1.112 ± 0.03	2.22HisLeu: 2.22 ± 0.045	0.546HisMet: 0.546 ± 0.023	0.746HisAsn: 0.746 ± 0.025	1.216HisPro: 1.216 ± 0.032	0.849HisGln: 0.849 ± 0.027	0.858HisArg: 0.858 ± 0.026	1.391HisSer: 1.391 ± 0.035	1.067HisThr: 1.067 ± 0.031	1.521HisVal: 1.521 ± 0.04	0.256HisTrp: 0.256 ± 0.015	0.842HisTyr: 0.842 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	6.01IleAla: 6.01 ± 0.084	0.694IleCys: 0.694 ± 0.025	4.318IleAsp: 4.318 ± 0.055	5.789IleGlu: 5.789 ± 0.077	3.437IlePhe: 3.437 ± 0.064	6.192IleGly: 6.192 ± 0.089	1.743IleHis: 1.743 ± 0.041	5.733IleIle: 5.733 ± 0.092	4.667IleLys: 4.667 ± 0.048	7.231IleLeu: 7.231 ± 0.096	1.756IleMet: 1.756 ± 0.046	3.279IleAsn: 3.279 ± 0.052	3.333IlePro: 3.333 ± 0.058	2.851IleGln: 2.851 ± 0.048	3.131IleArg: 3.131 ± 0.052	5.514IleSer: 5.514 ± 0.079	4.233IleThr: 4.233 ± 0.062	5.501IleVal: 5.501 ± 0.07	0.668IleTrp: 0.668 ± 0.027	2.565IleTyr: 2.565 ± 0.045	0.0IleXaa: 0.0 ± 0.0
Lys	4.778LysAla: 4.778 ± 0.067	0.302LysCys: 0.302 ± 0.017	3.906LysAsp: 3.906 ± 0.064	7.14LysGlu: 7.14 ± 0.079	1.923LysPhe: 1.923 ± 0.044	4.444LysGly: 4.444 ± 0.061	1.388LysHis: 1.388 ± 0.032	4.731LysIle: 4.731 ± 0.068	6.531LysLys: 6.531 ± 0.077	6.012LysLeu: 6.012 ± 0.085	2.114LysMet: 2.114 ± 0.044	3.635LysAsn: 3.635 ± 0.056	2.327LysPro: 2.327 ± 0.046	3.143LysGln: 3.143 ± 0.054	3.411LysArg: 3.411 ± 0.057	3.813LysSer: 3.813 ± 0.058	3.726LysThr: 3.726 ± 0.062	4.295LysVal: 4.295 ± 0.063	0.796LysTrp: 0.796 ± 0.026	2.033LysTyr: 2.033 ± 0.046	0.0LysXaa: 0.0 ± 0.0
Leu	7.227LeuAla: 7.227 ± 0.092	0.719LeuCys: 0.719 ± 0.025	4.836LeuAsp: 4.836 ± 0.069	6.502LeuGlu: 6.502 ± 0.088	5.189LeuPhe: 5.189 ± 0.096	6.353LeuGly: 6.353 ± 0.086	2.018LeuHis: 2.018 ± 0.043	7.58LeuIle: 7.58 ± 0.106	6.918LeuLys: 6.918 ± 0.08	10.638LeuLeu: 10.638 ± 0.135	2.417LeuMet: 2.417 ± 0.051	4.356LeuAsn: 4.356 ± 0.054	4.073LeuPro: 4.073 ± 0.063	3.621LeuGln: 3.621 ± 0.06	3.649LeuArg: 3.649 ± 0.057	7.378LeuSer: 7.378 ± 0.082	5.592LeuThr: 5.592 ± 0.075	5.967LeuVal: 5.967 ± 0.081	0.88LeuTrp: 0.88 ± 0.028	3.113LeuTyr: 3.113 ± 0.054	0.0LeuXaa: 0.0 ± 0.0
Met	1.991MetAla: 1.991 ± 0.047	0.154MetCys: 0.154 ± 0.011	1.334MetAsp: 1.334 ± 0.03	1.754MetGlu: 1.754 ± 0.036	1.057MetPhe: 1.057 ± 0.032	1.652MetGly: 1.652 ± 0.039	0.38MetHis: 0.38 ± 0.016	2.18MetIle: 2.18 ± 0.045	2.48MetLys: 2.48 ± 0.053	2.441MetLeu: 2.441 ± 0.044	0.761MetMet: 0.761 ± 0.027	1.584MetAsn: 1.584 ± 0.035	0.987MetPro: 0.987 ± 0.028	0.853MetGln: 0.853 ± 0.027	1.026MetArg: 1.026 ± 0.03	1.708MetSer: 1.708 ± 0.038	1.631MetThr: 1.631 ± 0.038	1.596MetVal: 1.596 ± 0.04	0.174MetTrp: 0.174 ± 0.01	0.711MetTyr: 0.711 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	2.632AsnAla: 2.632 ± 0.046	0.284AsnCys: 0.284 ± 0.015	2.328AsnAsp: 2.328 ± 0.04	3.779AsnGlu: 3.779 ± 0.051	1.607AsnPhe: 1.607 ± 0.036	3.433AsnGly: 3.433 ± 0.06	1.117AsnHis: 1.117 ± 0.031	3.165AsnIle: 3.165 ± 0.061	3.031AsnLys: 3.031 ± 0.055	3.846AsnLeu: 3.846 ± 0.055	1.143AsnMet: 1.143 ± 0.032	1.964AsnAsn: 1.964 ± 0.041	2.087AsnPro: 2.087 ± 0.048	2.119AsnGln: 2.119 ± 0.043	2.098AsnArg: 2.098 ± 0.045	2.457AsnSer: 2.457 ± 0.048	2.062AsnThr: 2.062 ± 0.043	2.912AsnVal: 2.912 ± 0.058	0.521AsnTrp: 0.521 ± 0.022	1.5AsnTyr: 1.5 ± 0.037	0.0AsnXaa: 0.0 ± 0.0
Pro	2.424ProAla: 2.424 ± 0.045	0.218ProCys: 0.218 ± 0.014	2.233ProAsp: 2.233 ± 0.052	3.209ProGlu: 3.209 ± 0.054	2.12ProPhe: 2.12 ± 0.042	2.374ProGly: 2.374 ± 0.053	0.861ProHis: 0.861 ± 0.028	2.763ProIle: 2.763 ± 0.049	2.18ProLys: 2.18 ± 0.048	3.591ProLeu: 3.591 ± 0.063	0.743ProMet: 0.743 ± 0.024	1.648ProAsn: 1.648 ± 0.032	1.006ProPro: 1.006 ± 0.031	1.065ProGln: 1.065 ± 0.032	1.104ProArg: 1.104 ± 0.029	2.45ProSer: 2.45 ± 0.043	1.824ProThr: 1.824 ± 0.041	2.88ProVal: 2.88 ± 0.051	0.409ProTrp: 0.409 ± 0.017	1.487ProTyr: 1.487 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	2.714GlnAla: 2.714 ± 0.055	0.165GlnCys: 0.165 ± 0.012	1.527GlnAsp: 1.527 ± 0.035	2.66GlnGlu: 2.66 ± 0.048	1.643GlnPhe: 1.643 ± 0.036	2.021GlnGly: 2.021 ± 0.045	0.747GlnHis: 0.747 ± 0.027	2.762GlnIle: 2.762 ± 0.048	2.833GlnLys: 2.833 ± 0.047	4.03GlnLeu: 4.03 ± 0.067	1.171GlnMet: 1.171 ± 0.033	1.661GlnAsn: 1.661 ± 0.04	1.302GlnPro: 1.302 ± 0.041	1.602GlnGln: 1.602 ± 0.051	1.391GlnArg: 1.391 ± 0.034	2.305GlnSer: 2.305 ± 0.044	2.084GlnThr: 2.084 ± 0.046	2.245GlnVal: 2.245 ± 0.043	0.365GlnTrp: 0.365 ± 0.017	1.227GlnTyr: 1.227 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	2.354ArgAla: 2.354 ± 0.05	0.293ArgCys: 0.293 ± 0.017	1.967ArgAsp: 1.967 ± 0.041	3.08ArgGlu: 3.08 ± 0.051	1.973ArgPhe: 1.973 ± 0.041	2.259ArgGly: 2.259 ± 0.042	0.816ArgHis: 0.816 ± 0.026	3.161ArgIle: 3.161 ± 0.058	3.315ArgLys: 3.315 ± 0.057	4.123ArgLeu: 4.123 ± 0.068	1.281ArgMet: 1.281 ± 0.034	1.916ArgAsn: 1.916 ± 0.041	1.275ArgPro: 1.275 ± 0.029	1.537ArgGln: 1.537 ± 0.036	1.746ArgArg: 1.746 ± 0.041	2.406ArgSer: 2.406 ± 0.045	2.084ArgThr: 2.084 ± 0.046	2.589ArgVal: 2.589 ± 0.048	0.404ArgTrp: 0.404 ± 0.019	1.572ArgTyr: 1.572 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	4.219SerAla: 4.219 ± 0.063	0.481SerCys: 0.481 ± 0.019	3.119SerAsp: 3.119 ± 0.049	4.321SerGlu: 4.321 ± 0.064	3.648SerPhe: 3.648 ± 0.058	4.842SerGly: 4.842 ± 0.071	1.364SerHis: 1.364 ± 0.039	5.367SerIle: 5.367 ± 0.062	4.089SerLys: 4.089 ± 0.057	6.692SerLeu: 6.692 ± 0.08	1.788SerMet: 1.788 ± 0.039	2.627SerAsn: 2.627 ± 0.047	2.304SerPro: 2.304 ± 0.044	1.957SerGln: 1.957 ± 0.039	2.411SerArg: 2.411 ± 0.05	4.437SerSer: 4.437 ± 0.068	3.023SerThr: 3.023 ± 0.049	4.374SerVal: 4.374 ± 0.061	0.705SerTrp: 0.705 ± 0.026	2.382SerTyr: 2.382 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	3.993ThrAla: 3.993 ± 0.053	0.349ThrCys: 0.349 ± 0.017	2.709ThrAsp: 2.709 ± 0.045	3.515ThrGlu: 3.515 ± 0.055	2.579ThrPhe: 2.579 ± 0.048	4.205ThrGly: 4.205 ± 0.062	1.019ThrHis: 1.019 ± 0.033	4.578ThrIle: 4.578 ± 0.062	3.373ThrLys: 3.373 ± 0.049	5.063ThrLeu: 5.063 ± 0.055	1.255ThrMet: 1.255 ± 0.036	2.34ThrAsn: 2.34 ± 0.05	2.202ThrPro: 2.202 ± 0.041	1.302ThrGln: 1.302 ± 0.032	1.815ThrArg: 1.815 ± 0.039	3.381ThrSer: 3.381 ± 0.061	2.615ThrThr: 2.615 ± 0.054	4.02ThrVal: 4.02 ± 0.057	0.518ThrTrp: 0.518 ± 0.02	1.852ThrTyr: 1.852 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	4.44ValAla: 4.44 ± 0.07	0.595ValCys: 0.595 ± 0.024	3.342ValAsp: 3.342 ± 0.053	4.387ValGlu: 4.387 ± 0.068	3.298ValPhe: 3.298 ± 0.058	4.17ValGly: 4.17 ± 0.075	1.356ValHis: 1.356 ± 0.034	5.811ValIle: 5.811 ± 0.085	4.596ValLys: 4.596 ± 0.059	6.791ValLeu: 6.791 ± 0.074	1.733ValMet: 1.733 ± 0.04	3.108ValAsn: 3.108 ± 0.054	2.578ValPro: 2.578 ± 0.044	2.212ValGln: 2.212 ± 0.049	2.585ValArg: 2.585 ± 0.048	4.651ValSer: 4.651 ± 0.069	4.001ValThr: 4.001 ± 0.052	4.459ValVal: 4.459 ± 0.076	0.685ValTrp: 0.685 ± 0.025	2.309ValTyr: 2.309 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	0.619TrpAla: 0.619 ± 0.022	0.075TrpCys: 0.075 ± 0.009	0.534TrpAsp: 0.534 ± 0.021	0.706TrpGlu: 0.706 ± 0.022	0.57TrpPhe: 0.57 ± 0.026	0.697TrpGly: 0.697 ± 0.025	0.229TrpHis: 0.229 ± 0.013	0.88TrpIle: 0.88 ± 0.03	0.747TrpLys: 0.747 ± 0.027	1.143TrpLeu: 1.143 ± 0.037	0.317TrpMet: 0.317 ± 0.019	0.592TrpAsn: 0.592 ± 0.022	0.251TrpPro: 0.251 ± 0.015	0.341TrpGln: 0.341 ± 0.019	0.432TrpArg: 0.432 ± 0.021	0.644TrpSer: 0.644 ± 0.024	0.571TrpThr: 0.571 ± 0.022	0.652TrpVal: 0.652 ± 0.025	0.143TrpTrp: 0.143 ± 0.011	0.356TrpTyr: 0.356 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.071TyrAla: 2.071 ± 0.043	0.328TyrCys: 0.328 ± 0.018	1.876TyrAsp: 1.876 ± 0.039	2.52TyrGlu: 2.52 ± 0.049	1.739TyrPhe: 1.739 ± 0.04	2.496TyrGly: 2.496 ± 0.049	0.877TyrHis: 0.877 ± 0.03	2.317TyrIle: 2.317 ± 0.046	2.156TyrLys: 2.156 ± 0.044	3.58TyrLeu: 3.58 ± 0.052	0.806TyrMet: 0.806 ± 0.026	1.334TyrAsn: 1.334 ± 0.033	1.41TyrPro: 1.41 ± 0.037	1.496TyrGln: 1.496 ± 0.035	1.624TyrArg: 1.624 ± 0.038	2.36TyrSer: 2.36 ± 0.041	1.81TyrThr: 1.81 ± 0.039	2.154TyrVal: 2.154 ± 0.039	0.414TyrTrp: 0.414 ± 0.021	1.387TyrTyr: 1.387 ± 0.037	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4231 proteins (1209575 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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