Klebsiella phage ST16-OXA48phi5.1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482MEN8|A0A482MEN8_9CAUD Putative CI OS=Klebsiella phage ST16-OXA48phi5.1 OX=2510481 PE=4 SV=1
MM1 pKa = 7.15ITGTTNYY8 pKa = 10.48DD9 pKa = 3.56DD10 pKa = 5.04VPDD13 pKa = 4.37VPCTLCGGYY22 pKa = 10.3YY23 pKa = 10.34KK24 pKa = 10.64ADD26 pKa = 3.55EE27 pKa = 5.0PEE29 pKa = 4.05SHH31 pKa = 7.43DD32 pKa = 4.43CDD34 pKa = 3.51EE35 pKa = 4.51EE36 pKa = 4.08AHH38 pKa = 6.65NEE40 pKa = 4.03IEE42 pKa = 5.02CDD44 pKa = 2.8ICGFKK49 pKa = 10.0STDD52 pKa = 3.28PEE54 pKa = 4.35GAHH57 pKa = 5.96YY58 pKa = 10.64CCEE61 pKa = 4.46DD62 pKa = 3.56NSDD65 pKa = 3.64EE66 pKa = 4.21

Molecular weight: 7.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482MEC3|A0A482MEC3_9CAUD Uncharacterized protein OS=Klebsiella phage ST16-OXA48phi5.1 OX=2510481 PE=4 SV=1
MM1 pKa = 7.18SRR3 pKa = 11.84DD4 pKa = 3.51MLEE7 pKa = 4.83LIRR10 pKa = 11.84DD11 pKa = 3.97RR12 pKa = 11.84WWKK15 pKa = 10.37LRR17 pKa = 11.84LFRR20 pKa = 11.84CRR22 pKa = 11.84GTVMTDD28 pKa = 2.85YY29 pKa = 10.98RR30 pKa = 11.84ILKK33 pKa = 9.33NFVRR37 pKa = 11.84IYY39 pKa = 10.63QSLGEE44 pKa = 4.2KK45 pKa = 10.15AA46 pKa = 3.82

Molecular weight: 5.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

16660

37

1109

210.9

23.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.282 ± 0.354

1.345 ± 0.168

6.026 ± 0.187

6.279 ± 0.287

3.379 ± 0.182

7.029 ± 0.297

1.801 ± 0.155

5.384 ± 0.196

5.03 ± 0.313

9.07 ± 0.395

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.557 ± 0.118

3.571 ± 0.187

4.31 ± 0.237

4.49 ± 0.384

6.255 ± 0.302

6.068 ± 0.256

6.104 ± 0.402

6.519 ± 0.266

1.711 ± 0.149

2.791 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski