Pseudidiomarina indica
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2066 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G6D9Y2|A0A1G6D9Y2_9GAMM Uncharacterized conserved protein YeaO DUF488 family OS=Pseudidiomarina indica OX=1159017 GN=SAMN02927930_01593 PE=4 SV=1
MM1 pKa = 7.9 EE2 pKa = 5.15 YY3 pKa = 8.87 NTSEE7 pKa = 4.54 LCDD10 pKa = 4.62 LYY12 pKa = 10.6 PDD14 pKa = 3.89 LVDD17 pKa = 3.44 VVEE20 pKa = 4.89 PMFIHH25 pKa = 5.87 YY26 pKa = 9.94 GGRR29 pKa = 11.84 LSFGGQLVVVKK40 pKa = 10.36 CYY42 pKa = 9.63 EE43 pKa = 4.1 DD44 pKa = 3.25 KK45 pKa = 11.46 GLIEE49 pKa = 5.44 EE50 pKa = 4.82 IVQQSGTGKK59 pKa = 10.2 VLLVDD64 pKa = 4.28 GGGSMRR70 pKa = 11.84 RR71 pKa = 11.84 ALIDD75 pKa = 3.17 IAIADD80 pKa = 3.87 MAFDD84 pKa = 4.57 NDD86 pKa = 3.66 WEE88 pKa = 4.71 GIICYY93 pKa = 10.35 GSVRR97 pKa = 11.84 DD98 pKa = 3.74 VDD100 pKa = 4.19 ALEE103 pKa = 4.22 EE104 pKa = 4.21 LDD106 pKa = 3.69 MGIMAIAAIPVGAASDD122 pKa = 4.12 GVGEE126 pKa = 4.06 VDD128 pKa = 3.57 VAVNFGGVTFLPEE141 pKa = 3.55 DD142 pKa = 3.57 HH143 pKa = 7.13 LYY145 pKa = 11.13 ADD147 pKa = 3.65 STGIIISPEE156 pKa = 3.88 PLDD159 pKa = 3.95 IEE161 pKa = 4.39
Molecular weight: 17.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.719
IPC_protein 3.706
Toseland 3.503
ProMoST 3.859
Dawson 3.706
Bjellqvist 3.897
Wikipedia 3.643
Rodwell 3.541
Grimsley 3.414
Solomon 3.694
Lehninger 3.643
Nozaki 3.821
DTASelect 4.037
Thurlkill 3.554
EMBOSS 3.643
Sillero 3.834
Patrickios 0.985
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.776
Protein with the highest isoelectric point:
>tr|A0A1G6BVJ2|A0A1G6BVJ2_9GAMM Membrane protein insertase YidC OS=Pseudidiomarina indica OX=1159017 GN=yidC PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 9.37 RR14 pKa = 11.84 NHH16 pKa = 5.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.31 NGRR28 pKa = 11.84 QILARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.95 GRR39 pKa = 11.84 KK40 pKa = 8.67 VLSAA44 pKa = 4.05
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2066
0
2066
680199
10
2163
329.2
36.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.085 ± 0.061
0.853 ± 0.015
5.215 ± 0.045
6.138 ± 0.057
3.802 ± 0.036
6.956 ± 0.047
2.546 ± 0.029
5.76 ± 0.038
3.66 ± 0.047
10.738 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.483 ± 0.026
3.377 ± 0.032
4.48 ± 0.034
5.685 ± 0.065
5.709 ± 0.042
5.374 ± 0.038
5.424 ± 0.034
7.349 ± 0.047
1.378 ± 0.025
2.988 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here