Pteropox virus
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 143 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B1MR96|A0A1B1MR96_9POXV S-s bond formation pathway protein OS=Pteropox virus OX=1873698 GN=PTPV-Aus-017 PE=4 SV=1
MM1 pKa = 7.23 MAQILNFVSNRR12 pKa = 11.84 FYY14 pKa = 10.93 YY15 pKa = 10.53 DD16 pKa = 2.86 RR17 pKa = 11.84 GNFEE21 pKa = 5.45 LEE23 pKa = 3.88 IQEE26 pKa = 5.24 GILDD30 pKa = 4.39 LARR33 pKa = 11.84 SIEE36 pKa = 4.1 AVANLPRR43 pKa = 11.84 QEE45 pKa = 4.12 GAVRR49 pKa = 11.84 TIHH52 pKa = 6.08 GDD54 pKa = 3.23 EE55 pKa = 4.25 YY56 pKa = 11.27 FVIEE60 pKa = 3.9 TARR63 pKa = 11.84 RR64 pKa = 11.84 QNGDD68 pKa = 3.3 RR69 pKa = 11.84 EE70 pKa = 4.33 EE71 pKa = 4.23 NNQQILQPDD80 pKa = 3.73 NQFDD84 pKa = 3.9 NLDD87 pKa = 3.86 GNVEE91 pKa = 4.01 IPLVQQNQQPVQPVLLPPPNQLVDD115 pKa = 4.2 DD116 pKa = 4.58 VQDD119 pKa = 3.72 QADD122 pKa = 4.22 SPMLAQSSNHH132 pKa = 5.74 MNTQEE137 pKa = 4.48 DD138 pKa = 4.6 VLSSDD143 pKa = 3.75 MLNLFDD149 pKa = 6.27 DD150 pKa = 4.68 IEE152 pKa = 4.38 WNDD155 pKa = 3.44 EE156 pKa = 4.37 FIQQVLNNPEE166 pKa = 4.21 TIFPISEE173 pKa = 4.37 FDD175 pKa = 3.64 PNMDD179 pKa = 3.32 ISCYY183 pKa = 10.64 EE184 pKa = 4.18 NLDD187 pKa = 3.84 DD188 pKa = 4.74 MM189 pKa = 5.96
Molecular weight: 21.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.155
IPC2_protein 3.668
IPC_protein 3.668
Toseland 3.452
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.592
Rodwell 3.49
Grimsley 3.363
Solomon 3.643
Lehninger 3.605
Nozaki 3.77
DTASelect 3.986
Thurlkill 3.503
EMBOSS 3.605
Sillero 3.783
Patrickios 3.287
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.752
Protein with the highest isoelectric point:
>tr|A0A1B1MRA8|A0A1B1MRA8_9POXV Serine/threonine-protein kinase OS=Pteropox virus OX=1873698 GN=PTPV-Aus-018 PE=3 SV=1
MM1 pKa = 7.69 NKK3 pKa = 9.93 RR4 pKa = 11.84 KK5 pKa = 9.7 LLTMIRR11 pKa = 11.84 FSIKK15 pKa = 10.38 LLNTNYY21 pKa = 10.65 SDD23 pKa = 4.04 VLCGKK28 pKa = 10.2 YY29 pKa = 9.99 SLKK32 pKa = 10.79 SVLPRR37 pKa = 11.84 IKK39 pKa = 10.39 KK40 pKa = 10.39 KK41 pKa = 10.58 LVCKK45 pKa = 9.27 YY46 pKa = 7.53 TAQMCNGSEE55 pKa = 3.51 SWYY58 pKa = 10.6 YY59 pKa = 10.24 IVLSLFKK66 pKa = 10.48 FVEE69 pKa = 4.06 LRR71 pKa = 11.84 KK72 pKa = 10.08 HH73 pKa = 6.1 ISCVNCDD80 pKa = 3.33 KK81 pKa = 10.84 KK82 pKa = 11.12 LSPRR86 pKa = 11.84 EE87 pKa = 3.79 FASLVNKK94 pKa = 9.78 CVQFLNVYY102 pKa = 10.22 DD103 pKa = 4.4 RR104 pKa = 11.84 YY105 pKa = 11.1 LL106 pKa = 4.86
Molecular weight: 12.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.153
IPC2_protein 9.209
IPC_protein 9.121
Toseland 9.823
ProMoST 9.545
Dawson 10.058
Bjellqvist 9.78
Wikipedia 10.218
Rodwell 10.511
Grimsley 10.116
Solomon 10.087
Lehninger 10.058
Nozaki 9.955
DTASelect 9.736
Thurlkill 9.911
EMBOSS 10.248
Sillero 9.999
Patrickios 9.897
IPC_peptide 10.087
IPC2_peptide 8.668
IPC2.peptide.svr19 8.015
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
143
0
143
42641
46
1286
298.2
34.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.491 ± 0.138
2.23 ± 0.095
5.57 ± 0.124
5.75 ± 0.233
5.077 ± 0.15
3.443 ± 0.131
1.834 ± 0.091
8.178 ± 0.195
8.142 ± 0.213
9.242 ± 0.191
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.387 ± 0.09
6.843 ± 0.153
3.654 ± 0.165
2.584 ± 0.097
3.612 ± 0.12
8.203 ± 0.151
6.351 ± 0.16
7.103 ± 0.134
0.657 ± 0.045
4.648 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here