Pectobacterium phage PPWS4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Pektosvirus; Pectobacterium virus PPWS4

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A250KA86|A0A250KA86_9CAUD Uncharacterized protein OS=Pectobacterium phage PPWS4 OX=1961914 PE=4 SV=1
MM1 pKa = 8.04AIQDD5 pKa = 4.18LNVDD9 pKa = 4.0SVAEE13 pKa = 3.94LDD15 pKa = 3.68AVNDD19 pKa = 3.33ILAAIGEE26 pKa = 4.51APVNTLMDD34 pKa = 4.69DD35 pKa = 3.87SNADD39 pKa = 3.1VANARR44 pKa = 11.84RR45 pKa = 11.84ILNTTNRR52 pKa = 11.84KK53 pKa = 8.07VQSKK57 pKa = 9.08GWTFNIEE64 pKa = 3.89EE65 pKa = 4.57GAVLQPDD72 pKa = 4.1VFSNLIDD79 pKa = 3.58YY80 pKa = 11.22SNDD83 pKa = 3.1YY84 pKa = 10.56LSVMSTGSTSVYY96 pKa = 9.97INRR99 pKa = 11.84GGYY102 pKa = 10.37LYY104 pKa = 10.78DD105 pKa = 3.41RR106 pKa = 11.84TALTDD111 pKa = 3.23QFTAPVSVNLIRR123 pKa = 11.84LRR125 pKa = 11.84AFDD128 pKa = 5.57DD129 pKa = 3.47MPEE132 pKa = 4.16CFRR135 pKa = 11.84TLIVTEE141 pKa = 4.15AARR144 pKa = 11.84MFNSRR149 pKa = 11.84YY150 pKa = 9.38FGAPEE155 pKa = 3.54IEE157 pKa = 3.84AVLRR161 pKa = 11.84SEE163 pKa = 3.99ILEE166 pKa = 4.17AKK168 pKa = 8.66AACMEE173 pKa = 3.97YY174 pKa = 10.86EE175 pKa = 3.95MDD177 pKa = 3.51YY178 pKa = 11.53GKK180 pKa = 11.19YY181 pKa = 10.9NMLDD185 pKa = 3.52GDD187 pKa = 4.16SHH189 pKa = 6.26VQGLLSRR196 pKa = 4.51

Molecular weight: 21.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A250KA84|A0A250KA84_9CAUD Uncharacterized protein OS=Pectobacterium phage PPWS4 OX=1961914 PE=4 SV=1
MM1 pKa = 7.48LKK3 pKa = 10.66APIKK7 pKa = 10.49ASTTIRR13 pKa = 11.84LSDD16 pKa = 4.27TVDD19 pKa = 2.55QWSRR23 pKa = 11.84RR24 pKa = 11.84IHH26 pKa = 5.76VNVRR30 pKa = 11.84NGKK33 pKa = 6.82PTLVYY38 pKa = 9.87RR39 pKa = 11.84WRR41 pKa = 11.84DD42 pKa = 3.35SKK44 pKa = 11.39SPKK47 pKa = 9.74SHH49 pKa = 4.99TQRR52 pKa = 11.84MTLSDD57 pKa = 3.85EE58 pKa = 4.1QVGRR62 pKa = 11.84LIAAMSIAAGVAADD76 pKa = 3.79NDD78 pKa = 3.77EE79 pKa = 4.18SRR81 pKa = 11.84LLVVAKK87 pKa = 10.58GRR89 pKa = 11.84DD90 pKa = 3.48AQQRR94 pKa = 11.84EE95 pKa = 4.12AGII98 pKa = 4.0

Molecular weight: 10.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

12183

29

1339

248.6

27.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.324 ± 0.503

0.977 ± 0.174

6.173 ± 0.227

6.525 ± 0.271

3.685 ± 0.241

7.765 ± 0.333

1.781 ± 0.175

5.015 ± 0.309

6.567 ± 0.304

8.175 ± 0.289

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.799 ± 0.181

4.605 ± 0.229

3.735 ± 0.155

3.907 ± 0.302

5.344 ± 0.207

6.328 ± 0.319

5.417 ± 0.271

7.018 ± 0.269

1.371 ± 0.157

3.488 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski