Amino acid dipepetide frequency for Pectobacterium phage PPWS4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.671AlaAla: 10.671 ± 1.49
0.985AlaCys: 0.985 ± 0.27
5.418AlaAsp: 5.418 ± 0.734
6.485AlaGlu: 6.485 ± 0.884
3.448AlaPhe: 3.448 ± 0.459
6.895AlaGly: 6.895 ± 0.821
0.821AlaHis: 0.821 ± 0.282
4.104AlaIle: 4.104 ± 0.648
5.746AlaLys: 5.746 ± 0.604
7.798AlaLeu: 7.798 ± 0.773
2.709AlaMet: 2.709 ± 0.461
4.761AlaAsn: 4.761 ± 0.52
4.104AlaPro: 4.104 ± 0.613
3.694AlaGln: 3.694 ± 0.537
5.254AlaArg: 5.254 ± 0.838
6.895AlaSer: 6.895 ± 0.778
3.776AlaThr: 3.776 ± 0.496
5.828AlaVal: 5.828 ± 0.708
0.739AlaTrp: 0.739 ± 0.316
3.119AlaTyr: 3.119 ± 0.469
0.0AlaXaa: 0.0 ± 0.0
Cys
0.821CysAla: 0.821 ± 0.295
0.164CysCys: 0.164 ± 0.123
0.575CysAsp: 0.575 ± 0.263
0.575CysGlu: 0.575 ± 0.236
0.575CysPhe: 0.575 ± 0.24
0.821CysGly: 0.821 ± 0.289
0.328CysHis: 0.328 ± 0.146
0.903CysIle: 0.903 ± 0.287
0.328CysLys: 0.328 ± 0.215
0.985CysLeu: 0.985 ± 0.375
0.164CysMet: 0.164 ± 0.113
0.246CysAsn: 0.246 ± 0.147
0.41CysPro: 0.41 ± 0.221
0.328CysGln: 0.328 ± 0.14
0.903CysArg: 0.903 ± 0.422
0.328CysSer: 0.328 ± 0.159
0.246CysThr: 0.246 ± 0.158
0.41CysVal: 0.41 ± 0.214
0.164CysTrp: 0.164 ± 0.123
0.493CysTyr: 0.493 ± 0.229
0.0CysXaa: 0.0 ± 0.0
Asp
5.582AspAla: 5.582 ± 0.58
0.575AspCys: 0.575 ± 0.22
3.366AspAsp: 3.366 ± 0.451
3.694AspGlu: 3.694 ± 0.5
2.873AspPhe: 2.873 ± 0.504
6.649AspGly: 6.649 ± 0.689
0.739AspHis: 0.739 ± 0.214
2.545AspIle: 2.545 ± 0.427
4.187AspLys: 4.187 ± 0.465
3.284AspLeu: 3.284 ± 0.53
2.298AspMet: 2.298 ± 0.385
3.201AspAsn: 3.201 ± 0.385
2.873AspPro: 2.873 ± 0.49
1.724AspGln: 1.724 ± 0.381
2.873AspArg: 2.873 ± 0.342
4.269AspSer: 4.269 ± 0.566
3.037AspThr: 3.037 ± 0.575
4.515AspVal: 4.515 ± 0.546
0.739AspTrp: 0.739 ± 0.281
2.709AspTyr: 2.709 ± 0.425
0.0AspXaa: 0.0 ± 0.0
Glu
6.813GluAla: 6.813 ± 0.651
0.575GluCys: 0.575 ± 0.26
4.104GluAsp: 4.104 ± 0.634
4.925GluGlu: 4.925 ± 0.876
2.873GluPhe: 2.873 ± 0.419
5.172GluGly: 5.172 ± 0.698
1.396GluHis: 1.396 ± 0.391
2.873GluIle: 2.873 ± 0.372
3.201GluLys: 3.201 ± 0.425
5.418GluLeu: 5.418 ± 0.552
1.396GluMet: 1.396 ± 0.335
2.052GluAsn: 2.052 ± 0.358
1.642GluPro: 1.642 ± 0.403
2.545GluGln: 2.545 ± 0.524
3.94GluArg: 3.94 ± 0.452
4.269GluSer: 4.269 ± 0.742
4.269GluThr: 4.269 ± 0.442
4.269GluVal: 4.269 ± 0.628
0.657GluTrp: 0.657 ± 0.233
2.873GluTyr: 2.873 ± 0.449
0.0GluXaa: 0.0 ± 0.0
Phe
2.627PheAla: 2.627 ± 0.426
0.493PheCys: 0.493 ± 0.229
2.873PheAsp: 2.873 ± 0.551
1.724PheGlu: 1.724 ± 0.321
0.903PhePhe: 0.903 ± 0.234
2.873PheGly: 2.873 ± 0.487
0.739PheHis: 0.739 ± 0.223
1.97PheIle: 1.97 ± 0.406
2.381PheLys: 2.381 ± 0.523
3.284PheLeu: 3.284 ± 0.576
1.149PheMet: 1.149 ± 0.282
1.56PheAsn: 1.56 ± 0.284
1.642PhePro: 1.642 ± 0.439
1.067PheGln: 1.067 ± 0.197
2.052PheArg: 2.052 ± 0.368
2.298PheSer: 2.298 ± 0.385
3.119PheThr: 3.119 ± 0.505
2.381PheVal: 2.381 ± 0.294
0.493PheTrp: 0.493 ± 0.178
1.231PheTyr: 1.231 ± 0.233
0.0PheXaa: 0.0 ± 0.0
Gly
7.47GlyAla: 7.47 ± 0.733
1.067GlyCys: 1.067 ± 0.454
5.007GlyAsp: 5.007 ± 0.725
4.515GlyGlu: 4.515 ± 0.526
3.53GlyPhe: 3.53 ± 0.474
5.089GlyGly: 5.089 ± 0.674
0.985GlyHis: 0.985 ± 0.241
4.351GlyIle: 4.351 ± 0.522
5.582GlyLys: 5.582 ± 0.732
7.06GlyLeu: 7.06 ± 0.708
1.642GlyMet: 1.642 ± 0.302
3.612GlyAsn: 3.612 ± 0.431
0.493GlyPro: 0.493 ± 0.234
3.119GlyGln: 3.119 ± 0.481
4.187GlyArg: 4.187 ± 0.555
5.007GlySer: 5.007 ± 0.62
4.515GlyThr: 4.515 ± 0.587
4.925GlyVal: 4.925 ± 0.673
1.724GlyTrp: 1.724 ± 0.382
3.776GlyTyr: 3.776 ± 0.598
0.0GlyXaa: 0.0 ± 0.0
His
0.985HisAla: 0.985 ± 0.277
0.328HisCys: 0.328 ± 0.135
1.067HisAsp: 1.067 ± 0.302
0.903HisGlu: 0.903 ± 0.274
0.657HisPhe: 0.657 ± 0.185
0.903HisGly: 0.903 ± 0.217
0.164HisHis: 0.164 ± 0.104
1.149HisIle: 1.149 ± 0.249
0.985HisLys: 0.985 ± 0.326
1.97HisLeu: 1.97 ± 0.392
0.985HisMet: 0.985 ± 0.29
0.985HisAsn: 0.985 ± 0.22
0.493HisPro: 0.493 ± 0.196
0.246HisGln: 0.246 ± 0.122
0.903HisArg: 0.903 ± 0.257
1.396HisSer: 1.396 ± 0.289
1.067HisThr: 1.067 ± 0.351
1.231HisVal: 1.231 ± 0.285
0.575HisTrp: 0.575 ± 0.195
0.739HisTyr: 0.739 ± 0.224
0.0HisXaa: 0.0 ± 0.0
Ile
4.351IleAla: 4.351 ± 0.578
0.657IleCys: 0.657 ± 0.243
2.627IleAsp: 2.627 ± 0.371
3.94IleGlu: 3.94 ± 0.429
1.231IlePhe: 1.231 ± 0.259
3.448IleGly: 3.448 ± 0.533
1.231IleHis: 1.231 ± 0.302
2.545IleIle: 2.545 ± 0.469
3.448IleLys: 3.448 ± 0.54
4.843IleLeu: 4.843 ± 0.913
1.56IleMet: 1.56 ± 0.395
2.216IleAsn: 2.216 ± 0.427
2.873IlePro: 2.873 ± 0.448
1.724IleGln: 1.724 ± 0.389
3.858IleArg: 3.858 ± 0.591
2.545IleSer: 2.545 ± 0.492
2.791IleThr: 2.791 ± 0.362
2.298IleVal: 2.298 ± 0.321
0.575IleTrp: 0.575 ± 0.212
1.396IleTyr: 1.396 ± 0.395
0.0IleXaa: 0.0 ± 0.0
Lys
8.455LysAla: 8.455 ± 1.081
0.575LysCys: 0.575 ± 0.206
3.119LysAsp: 3.119 ± 0.355
4.433LysGlu: 4.433 ± 0.824
2.873LysPhe: 2.873 ± 0.544
5.582LysGly: 5.582 ± 0.618
1.806LysHis: 1.806 ± 0.435
2.298LysIle: 2.298 ± 0.352
3.858LysLys: 3.858 ± 0.542
5.5LysLeu: 5.5 ± 0.674
1.478LysMet: 1.478 ± 0.353
1.806LysAsn: 1.806 ± 0.465
2.627LysPro: 2.627 ± 0.454
2.545LysGln: 2.545 ± 0.433
3.448LysArg: 3.448 ± 0.563
3.94LysSer: 3.94 ± 0.548
2.545LysThr: 2.545 ± 0.364
5.5LysVal: 5.5 ± 0.616
0.903LysTrp: 0.903 ± 0.234
2.381LysTyr: 2.381 ± 0.386
0.0LysXaa: 0.0 ± 0.0
Leu
7.963LeuAla: 7.963 ± 1.237
0.657LeuCys: 0.657 ± 0.179
4.679LeuAsp: 4.679 ± 0.6
5.992LeuGlu: 5.992 ± 0.707
1.97LeuPhe: 1.97 ± 0.363
5.664LeuGly: 5.664 ± 0.859
0.821LeuHis: 0.821 ± 0.204
4.515LeuIle: 4.515 ± 0.803
6.895LeuLys: 6.895 ± 0.647
5.828LeuLeu: 5.828 ± 0.811
3.037LeuMet: 3.037 ± 0.437
4.679LeuAsn: 4.679 ± 0.47
2.955LeuPro: 2.955 ± 0.527
3.366LeuGln: 3.366 ± 0.501
5.254LeuArg: 5.254 ± 0.439
5.172LeuSer: 5.172 ± 0.684
4.022LeuThr: 4.022 ± 0.557
4.597LeuVal: 4.597 ± 0.493
1.231LeuTrp: 1.231 ± 0.377
2.463LeuTyr: 2.463 ± 0.405
0.0LeuXaa: 0.0 ± 0.0
Met
3.612MetAla: 3.612 ± 0.471
0.164MetCys: 0.164 ± 0.128
1.231MetAsp: 1.231 ± 0.312
1.642MetGlu: 1.642 ± 0.327
1.231MetPhe: 1.231 ± 0.275
2.052MetGly: 2.052 ± 0.336
0.493MetHis: 0.493 ± 0.197
1.067MetIle: 1.067 ± 0.296
1.231MetLys: 1.231 ± 0.283
2.791MetLeu: 2.791 ± 0.385
0.41MetMet: 0.41 ± 0.194
1.724MetAsn: 1.724 ± 0.366
1.067MetPro: 1.067 ± 0.283
1.478MetGln: 1.478 ± 0.381
1.396MetArg: 1.396 ± 0.354
1.642MetSer: 1.642 ± 0.419
1.888MetThr: 1.888 ± 0.316
1.806MetVal: 1.806 ± 0.378
0.328MetTrp: 0.328 ± 0.119
0.739MetTyr: 0.739 ± 0.224
0.0MetXaa: 0.0 ± 0.0
Asn
2.791AsnAla: 2.791 ± 0.466
0.328AsnCys: 0.328 ± 0.199
3.776AsnAsp: 3.776 ± 0.588
2.545AsnGlu: 2.545 ± 0.461
1.478AsnPhe: 1.478 ± 0.358
4.679AsnGly: 4.679 ± 0.629
0.821AsnHis: 0.821 ± 0.261
2.545AsnIle: 2.545 ± 0.44
3.037AsnLys: 3.037 ± 0.467
3.448AsnLeu: 3.448 ± 0.635
1.067AsnMet: 1.067 ± 0.304
1.313AsnAsn: 1.313 ± 0.384
3.53AsnPro: 3.53 ± 0.618
1.806AsnGln: 1.806 ± 0.383
2.134AsnArg: 2.134 ± 0.429
2.463AsnSer: 2.463 ± 0.507
2.709AsnThr: 2.709 ± 0.541
3.612AsnVal: 3.612 ± 0.49
0.575AsnTrp: 0.575 ± 0.195
1.396AsnTyr: 1.396 ± 0.282
0.0AsnXaa: 0.0 ± 0.0
Pro
2.955ProAla: 2.955 ± 0.408
0.246ProCys: 0.246 ± 0.118
2.545ProAsp: 2.545 ± 0.33
4.022ProGlu: 4.022 ± 0.607
1.56ProPhe: 1.56 ± 0.372
0.0ProGly: 0.0 ± 0.0
0.575ProHis: 0.575 ± 0.214
1.724ProIle: 1.724 ± 0.42
2.791ProLys: 2.791 ± 0.495
2.134ProLeu: 2.134 ± 0.448
1.067ProMet: 1.067 ± 0.338
3.284ProAsn: 3.284 ± 0.525
1.313ProPro: 1.313 ± 0.345
1.56ProGln: 1.56 ± 0.35
1.806ProArg: 1.806 ± 0.309
2.791ProSer: 2.791 ± 0.488
2.052ProThr: 2.052 ± 0.379
2.873ProVal: 2.873 ± 0.44
0.575ProTrp: 0.575 ± 0.148
1.478ProTyr: 1.478 ± 0.47
0.0ProXaa: 0.0 ± 0.0
Gln
4.269GlnAla: 4.269 ± 0.738
0.493GlnCys: 0.493 ± 0.194
2.873GlnAsp: 2.873 ± 0.707
2.381GlnGlu: 2.381 ± 0.512
1.806GlnPhe: 1.806 ± 0.294
2.873GlnGly: 2.873 ± 0.469
0.821GlnHis: 0.821 ± 0.212
2.463GlnIle: 2.463 ± 0.532
1.806GlnLys: 1.806 ± 0.346
3.119GlnLeu: 3.119 ± 0.456
0.985GlnMet: 0.985 ± 0.332
1.231GlnAsn: 1.231 ± 0.317
1.97GlnPro: 1.97 ± 0.366
2.298GlnGln: 2.298 ± 0.454
1.806GlnArg: 1.806 ± 0.368
2.463GlnSer: 2.463 ± 0.483
1.56GlnThr: 1.56 ± 0.265
1.97GlnVal: 1.97 ± 0.348
0.821GlnTrp: 0.821 ± 0.246
1.067GlnTyr: 1.067 ± 0.427
0.0GlnXaa: 0.0 ± 0.0
Arg
4.351ArgAla: 4.351 ± 0.555
0.739ArgCys: 0.739 ± 0.238
4.433ArgAsp: 4.433 ± 0.568
3.694ArgGlu: 3.694 ± 0.463
1.396ArgPhe: 1.396 ± 0.362
4.022ArgGly: 4.022 ± 0.454
0.903ArgHis: 0.903 ± 0.294
2.381ArgIle: 2.381 ± 0.37
3.612ArgLys: 3.612 ± 0.656
5.172ArgLeu: 5.172 ± 0.545
2.052ArgMet: 2.052 ± 0.399
2.134ArgAsn: 2.134 ± 0.312
1.313ArgPro: 1.313 ± 0.279
2.298ArgGln: 2.298 ± 0.462
1.888ArgArg: 1.888 ± 0.353
3.858ArgSer: 3.858 ± 0.639
3.037ArgThr: 3.037 ± 0.364
3.694ArgVal: 3.694 ± 0.532
0.657ArgTrp: 0.657 ± 0.239
1.806ArgTyr: 1.806 ± 0.36
0.0ArgXaa: 0.0 ± 0.0
Ser
5.418SerAla: 5.418 ± 0.495
0.739SerCys: 0.739 ± 0.277
3.858SerAsp: 3.858 ± 0.648
2.709SerGlu: 2.709 ± 0.501
2.709SerPhe: 2.709 ± 0.372
7.06SerGly: 7.06 ± 1.018
1.642SerHis: 1.642 ± 0.414
3.119SerIle: 3.119 ± 0.507
3.776SerLys: 3.776 ± 0.563
4.351SerLeu: 4.351 ± 0.575
1.313SerMet: 1.313 ± 0.323
2.955SerAsn: 2.955 ± 0.448
2.052SerPro: 2.052 ± 0.421
2.627SerGln: 2.627 ± 0.481
2.873SerArg: 2.873 ± 0.45
4.269SerSer: 4.269 ± 0.596
3.776SerThr: 3.776 ± 0.567
5.172SerVal: 5.172 ± 0.617
0.739SerTrp: 0.739 ± 0.227
2.134SerTyr: 2.134 ± 0.49
0.0SerXaa: 0.0 ± 0.0
Thr
4.843ThrAla: 4.843 ± 0.633
0.328ThrCys: 0.328 ± 0.21
2.709ThrAsp: 2.709 ± 0.516
2.873ThrGlu: 2.873 ± 0.389
1.806ThrPhe: 1.806 ± 0.385
4.022ThrGly: 4.022 ± 0.451
0.985ThrHis: 0.985 ± 0.243
3.201ThrIle: 3.201 ± 0.537
4.597ThrLys: 4.597 ± 0.472
5.172ThrLeu: 5.172 ± 0.693
0.903ThrMet: 0.903 ± 0.263
1.888ThrAsn: 1.888 ± 0.332
2.627ThrPro: 2.627 ± 0.358
1.642ThrGln: 1.642 ± 0.408
2.627ThrArg: 2.627 ± 0.481
3.612ThrSer: 3.612 ± 0.549
3.037ThrThr: 3.037 ± 0.514
4.843ThrVal: 4.843 ± 0.649
0.903ThrTrp: 0.903 ± 0.215
1.56ThrTyr: 1.56 ± 0.427
0.0ThrXaa: 0.0 ± 0.0
Val
5.418ValAla: 5.418 ± 0.584
0.328ValCys: 0.328 ± 0.282
3.94ValAsp: 3.94 ± 0.488
4.679ValGlu: 4.679 ± 0.717
2.545ValPhe: 2.545 ± 0.509
5.089ValGly: 5.089 ± 0.61
1.313ValHis: 1.313 ± 0.319
3.776ValIle: 3.776 ± 0.566
5.664ValLys: 5.664 ± 0.662
5.172ValLeu: 5.172 ± 0.793
2.052ValMet: 2.052 ± 0.456
4.433ValAsn: 4.433 ± 0.69
1.97ValPro: 1.97 ± 0.421
3.201ValGln: 3.201 ± 0.382
3.284ValArg: 3.284 ± 0.57
3.94ValSer: 3.94 ± 0.63
4.269ValThr: 4.269 ± 0.556
6.239ValVal: 6.239 ± 0.917
0.657ValTrp: 0.657 ± 0.232
2.216ValTyr: 2.216 ± 0.416
0.0ValXaa: 0.0 ± 0.0
Trp
1.149TrpAla: 1.149 ± 0.344
0.164TrpCys: 0.164 ± 0.111
0.657TrpAsp: 0.657 ± 0.201
0.985TrpGlu: 0.985 ± 0.306
0.41TrpPhe: 0.41 ± 0.179
1.149TrpGly: 1.149 ± 0.284
0.328TrpHis: 0.328 ± 0.226
0.493TrpIle: 0.493 ± 0.209
0.821TrpLys: 0.821 ± 0.293
1.642TrpLeu: 1.642 ± 0.328
0.575TrpMet: 0.575 ± 0.213
0.739TrpAsn: 0.739 ± 0.246
0.164TrpPro: 0.164 ± 0.091
0.41TrpGln: 0.41 ± 0.17
1.149TrpArg: 1.149 ± 0.304
0.575TrpSer: 0.575 ± 0.215
0.657TrpThr: 0.657 ± 0.287
1.149TrpVal: 1.149 ± 0.431
0.328TrpTrp: 0.328 ± 0.133
0.164TrpTyr: 0.164 ± 0.1
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.709TyrAla: 2.709 ± 0.648
0.164TyrCys: 0.164 ± 0.114
2.873TyrAsp: 2.873 ± 0.53
2.545TyrGlu: 2.545 ± 0.365
0.985TyrPhe: 0.985 ± 0.317
3.612TyrGly: 3.612 ± 0.379
0.903TyrHis: 0.903 ± 0.272
2.134TyrIle: 2.134 ± 0.424
1.724TyrLys: 1.724 ± 0.403
2.791TyrLeu: 2.791 ± 0.591
1.067TyrMet: 1.067 ± 0.359
1.231TyrAsn: 1.231 ± 0.303
1.231TyrPro: 1.231 ± 0.26
1.396TyrGln: 1.396 ± 0.333
1.806TyrArg: 1.806 ± 0.303
1.56TyrSer: 1.56 ± 0.362
1.806TyrThr: 1.806 ± 0.475
2.873TyrVal: 2.873 ± 0.526
0.328TyrTrp: 0.328 ± 0.178
1.149TyrTyr: 1.149 ± 0.324
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (12183 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski