Chimaeribacter arupi
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4159 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2N5EPZ0|A0A2N5EPZ0_9GAMM EamA family transporter OS=Chimaeribacter arupi OX=2060066 GN=CYR34_07315 PE=4 SV=1
MM1 pKa = 7.38 NNGDD5 pKa = 3.62 NGMDD9 pKa = 3.97 IIYY12 pKa = 10.7 SLLNKK17 pKa = 8.54 RR18 pKa = 11.84 QSMTTATLFSRR29 pKa = 11.84 QTGNLISQIIDD40 pKa = 3.22 ASQVISLTEE49 pKa = 3.77 PSVITINNARR59 pKa = 11.84 GWATGYY65 pKa = 10.4 SRR67 pKa = 11.84 SGDD70 pKa = 3.88 DD71 pKa = 5.43 LSLATQDD78 pKa = 3.53 GTTLQYY84 pKa = 11.2 RR85 pKa = 11.84 HH86 pKa = 6.16 FFAAGSAGTLVFDD99 pKa = 5.5 DD100 pKa = 4.75 GSGPATQAVFSHH112 pKa = 7.23 DD113 pKa = 3.97 AGSSSLTPVFKK124 pKa = 10.42 AYY126 pKa = 9.52 HH127 pKa = 6.23 APVEE131 pKa = 4.2 AAGAARR137 pKa = 11.84 STQASAADD145 pKa = 3.53 INAVADD151 pKa = 3.47 VDD153 pKa = 4.58 AADD156 pKa = 4.35 TPTITINTFAGDD168 pKa = 3.75 NIINYY173 pKa = 10.21 AEE175 pKa = 4.27 GQVEE179 pKa = 4.29 QVLSGTTTGIEE190 pKa = 4.07 AGQSLTVAFNSIVSFWTEE208 pKa = 3.71 VQADD212 pKa = 4.35 GSWQLTLNAASMYY225 pKa = 10.93 DD226 pKa = 3.37 LLRR229 pKa = 11.84 YY230 pKa = 9.9 GSTEE234 pKa = 3.34 ITAEE238 pKa = 4.05 SANAAGEE245 pKa = 4.16 EE246 pKa = 4.36 ATVSVPFQVLSDD258 pKa = 3.83 WPSVTLNAFAGDD270 pKa = 3.95 NVISGPEE277 pKa = 3.83 DD278 pKa = 3.13 QATQILSGSTSRR290 pKa = 11.84 VEE292 pKa = 4.09 AGQTVTLTLNGNTYY306 pKa = 8.73 TATVDD311 pKa = 3.05 ADD313 pKa = 4.45 GYY315 pKa = 10.27 WRR317 pKa = 11.84 TSVPAADD324 pKa = 3.81 VAALPDD330 pKa = 4.03 GAQLVATVTNALGRR344 pKa = 11.84 EE345 pKa = 4.26 AGDD348 pKa = 3.66 SATLTVNSSLPSLTVDD364 pKa = 4.31 PFATDD369 pKa = 3.22 NVLSDD374 pKa = 5.16 AEE376 pKa = 4.17 AQSAQTLSGTTTGVEE391 pKa = 3.68 AGQAVTVTLNEE402 pKa = 3.81 ITYY405 pKa = 8.07 STTVAADD412 pKa = 3.71 GSFSLSVPSADD423 pKa = 5.09 LLALYY428 pKa = 10.32 SGQTTIAARR437 pKa = 11.84 AANLAGQEE445 pKa = 4.37 TQGSEE450 pKa = 3.55 ILTVDD455 pKa = 4.77 FATARR460 pKa = 11.84 LVVDD464 pKa = 4.72 PVAGDD469 pKa = 3.5 NYY471 pKa = 10.3 INASEE476 pKa = 4.05 SSYY479 pKa = 11.35 LVLSGTTVDD488 pKa = 3.83 VPAGSWVVITFNDD501 pKa = 4.06 RR502 pKa = 11.84 IFRR505 pKa = 11.84 AEE507 pKa = 3.96 VEE509 pKa = 4.46 ADD511 pKa = 4.04 GSWSTYY517 pKa = 10.53 VSDD520 pKa = 4.46 LVVNSFPDD528 pKa = 3.57 GQYY531 pKa = 10.46 PITFSAPGSAPVTLDD546 pKa = 2.86 VGLYY550 pKa = 9.84 IDD552 pKa = 4.82 NQAQLNVQIDD562 pKa = 4.02 TLFGDD567 pKa = 4.79 DD568 pKa = 3.8 VLTPGEE574 pKa = 4.15 TLQDD578 pKa = 3.49 QVLTGTTGITGAGQTISAEE597 pKa = 4.26 LEE599 pKa = 4.48 GVTHH603 pKa = 6.89 TGSVDD608 pKa = 3.2 NDD610 pKa = 4.08 GNWTITVPAAALQALQNPNGYY631 pKa = 10.36 VYY633 pKa = 10.92 VQAADD638 pKa = 3.58 LAGNSIAVGRR648 pKa = 11.84 SFEE651 pKa = 4.91 LVFSPATLTIDD662 pKa = 5.46 PITGDD667 pKa = 3.91 NILTAAEE674 pKa = 4.2 TATPLTVSGVSYY686 pKa = 10.23 RR687 pKa = 11.84 VPEE690 pKa = 4.32 GVTITVTLNGKK701 pKa = 9.56 EE702 pKa = 4.13 YY703 pKa = 7.83 TTTAPGNDD711 pKa = 3.24 TWSVQVPAADD721 pKa = 3.97 LAGLTDD727 pKa = 3.91 GVYY730 pKa = 9.55 TVTAATINIDD740 pKa = 3.91 LEE742 pKa = 4.51 PVTTQQDD749 pKa = 3.89 LTVDD753 pKa = 4.55 LAPAPAFSVDD763 pKa = 3.88 PVTGDD768 pKa = 3.9 DD769 pKa = 3.4 ILYY772 pKa = 10.17 VDD774 pKa = 4.11 EE775 pKa = 4.55 TSGEE779 pKa = 3.96 IVITGRR785 pKa = 11.84 APVSAVGGSVTVTAFDD801 pKa = 4.04 DD802 pKa = 3.54 PAYY805 pKa = 10.44 RR806 pKa = 11.84 YY807 pKa = 7.59 TGTVAADD814 pKa = 3.79 GTWQVTVPEE823 pKa = 4.39 YY824 pKa = 11.36 NFGGTSNGVYY834 pKa = 9.85 TLVAEE839 pKa = 4.71 LTAPDD844 pKa = 3.91 GTTTTTTHH852 pKa = 7.66 DD853 pKa = 2.94 ITINVWPFSGDD864 pKa = 3.29 FYY866 pKa = 11.49 FDD868 pKa = 4.79 DD869 pKa = 4.02 FTGDD873 pKa = 3.23 STLTAEE879 pKa = 5.45 EE880 pKa = 4.22 RR881 pKa = 11.84 TQDD884 pKa = 3.45 QVFSGTFASEE894 pKa = 4.09 EE895 pKa = 4.09 NDD897 pKa = 3.29 VSGLGITVYY906 pKa = 9.7 MHH908 pKa = 7.42 DD909 pKa = 4.17 NDD911 pKa = 4.37 GQTFAYY917 pKa = 9.41 TGTTDD922 pKa = 3.67 GNGRR926 pKa = 11.84 WSVTIPAADD935 pKa = 3.64 LQQLSEE941 pKa = 4.02 GWSYY945 pKa = 7.65 MTATATDD952 pKa = 3.42 AAGNGASSSRR962 pKa = 11.84 EE963 pKa = 3.22 ADD965 pKa = 3.78 FAVLPATGGTITVNPIAGDD984 pKa = 3.62 NVLTADD990 pKa = 4.93 EE991 pKa = 4.81 ISDD994 pKa = 3.69 GLTISGSSTYY1004 pKa = 9.8 MGEE1007 pKa = 4.17 RR1008 pKa = 11.84 YY1009 pKa = 7.47 TQIEE1013 pKa = 4.17 VEE1015 pKa = 4.15 INGTLRR1021 pKa = 11.84 IYY1023 pKa = 11.25 ADD1025 pKa = 4.12 YY1026 pKa = 10.95 EE1027 pKa = 4.49 SDD1029 pKa = 2.92 WSVTFSRR1036 pKa = 11.84 EE1037 pKa = 4.0 FIATLPDD1044 pKa = 3.67 GPLTVTASGYY1054 pKa = 10.15 DD1055 pKa = 3.34 LSGNRR1060 pKa = 11.84 VTTTATLEE1068 pKa = 4.25 VNLSDD1073 pKa = 4.91 VSTLTLDD1080 pKa = 3.63 PVTGDD1085 pKa = 3.92 DD1086 pKa = 4.89 VISADD1091 pKa = 3.57 EE1092 pKa = 4.33 AGGEE1096 pKa = 4.07 IVITGRR1102 pKa = 11.84 APLSTQGGTVTVTAFDD1118 pKa = 4.13 DD1119 pKa = 3.53 PAYY1122 pKa = 10.4 RR1123 pKa = 11.84 YY1124 pKa = 6.88 TATVEE1129 pKa = 4.89 EE1130 pKa = 5.09 GGTWQATVPAGAFSGRR1146 pKa = 11.84 ANGVYY1151 pKa = 9.79 TLVAEE1156 pKa = 4.71 LTAPDD1161 pKa = 3.88 GTVTTLNHH1169 pKa = 6.92 DD1170 pKa = 3.68 VTSNQLPFSGGFAFYY1185 pKa = 11.02 VFAGDD1190 pKa = 3.78 DD1191 pKa = 3.45 VLNAEE1196 pKa = 4.87 EE1197 pKa = 4.0 QAQDD1201 pKa = 3.46 QILEE1205 pKa = 4.36 GEE1207 pKa = 4.52 VFSDD1211 pKa = 4.67 DD1212 pKa = 3.79 GGNVAGLAVTIQMTTFDD1229 pKa = 4.33 GQQFTYY1235 pKa = 8.05 TTTTGEE1241 pKa = 3.69 NGYY1244 pKa = 8.39 WQVTLPSSDD1253 pKa = 4.43 LEE1255 pKa = 3.95 QLRR1258 pKa = 11.84 NGWVNLVGTVADD1270 pKa = 4.16 GAGNTLSNEE1279 pKa = 4.15 SQFTVLAGIGTLTINPIAGDD1299 pKa = 3.68 NVLTADD1305 pKa = 4.31 EE1306 pKa = 4.73 IGNGLVVSGSGEE1318 pKa = 4.1 FLDD1321 pKa = 4.23 MPLSLEE1327 pKa = 3.99 VTINGVSQGFSDD1339 pKa = 4.83 YY1340 pKa = 11.38 SSDD1343 pKa = 2.81 WSVTFTPQQLAGFPDD1358 pKa = 4.56 GPLTVTVTAYY1368 pKa = 10.36 DD1369 pKa = 3.37 IEE1371 pKa = 4.89 FNLITATSTLTVDD1384 pKa = 5.3 LGDD1387 pKa = 4.16 LQPLTFNPFGGDD1399 pKa = 3.55 DD1400 pKa = 3.7 NYY1402 pKa = 11.42 LDD1404 pKa = 4.19 EE1405 pKa = 6.73 DD1406 pKa = 4.18 EE1407 pKa = 5.81 LEE1409 pKa = 4.27 SDD1411 pKa = 4.1 QLLSGQAGNLAAGSIVTLTLGEE1433 pKa = 4.01 FTYY1436 pKa = 10.56 QAEE1439 pKa = 4.42 VQADD1443 pKa = 3.89 HH1444 pKa = 6.48 SWSVLIPSADD1454 pKa = 3.86 LQTLPNDD1461 pKa = 3.22 ATTGISATVTDD1472 pKa = 4.25 ADD1474 pKa = 4.36 GNLLAAGSHH1483 pKa = 5.26 TFLLYY1488 pKa = 8.6 RR1489 pKa = 11.84 TPGEE1493 pKa = 4.1 LNIDD1497 pKa = 3.73 TLAGNGILTADD1508 pKa = 4.12 EE1509 pKa = 4.25 ATVPLVISGTITNGRR1524 pKa = 11.84 VGAAVTVDD1532 pKa = 4.07 LNGHH1536 pKa = 7.27 LYY1538 pKa = 8.17 QTTVTASDD1546 pKa = 4.28 GSWQVSVPVEE1556 pKa = 4.34 DD1557 pKa = 4.74 LAQLGNTEE1565 pKa = 4.25 YY1566 pKa = 10.01 WVSATSTDD1574 pKa = 3.83 LSGNEE1579 pKa = 3.75 INGLNLLVVDD1589 pKa = 5.45 LYY1591 pKa = 11.84 VPVITLDD1598 pKa = 4.31 PIAGDD1603 pKa = 3.86 NIINAAEE1610 pKa = 3.92 AAAGVVLSGQSSEE1623 pKa = 4.17 AGGVVSVQLNGVAYY1637 pKa = 9.05 EE1638 pKa = 4.16 AGVDD1642 pKa = 4.83 EE1643 pKa = 4.73 EE1644 pKa = 5.53 GSWQLALPNSALAEE1658 pKa = 4.33 LEE1660 pKa = 4.07 DD1661 pKa = 3.94 GRR1663 pKa = 11.84 YY1664 pKa = 7.65 TLTLSQTGFNGQTTTVTEE1682 pKa = 4.2 SLLLDD1687 pKa = 4.37 ADD1689 pKa = 3.96 PANRR1693 pKa = 11.84 PVLRR1697 pKa = 11.84 IHH1699 pKa = 7.15 TISHH1703 pKa = 6.77 DD1704 pKa = 3.65 NVLNGAEE1711 pKa = 4.21 LQSDD1715 pKa = 3.85 QIITGSSEE1723 pKa = 3.94 NVEE1726 pKa = 4.29 SGQTLSLKK1734 pKa = 10.42 IGGVTYY1740 pKa = 7.72 TTQVQPGGGWSLIVPAEE1757 pKa = 4.11 DD1758 pKa = 3.93 LAALGDD1764 pKa = 3.86 GGLTLRR1770 pKa = 11.84 AGVTDD1775 pKa = 3.93 ASGNRR1780 pKa = 11.84 ATASRR1785 pKa = 11.84 NLTIKK1790 pKa = 10.26 SDD1792 pKa = 3.85 RR1793 pKa = 11.84 DD1794 pKa = 3.58 GLSIDD1799 pKa = 4.35 PVTGDD1804 pKa = 3.77 NLINAADD1811 pKa = 3.8 AEE1813 pKa = 4.55 GAITLTGHH1821 pKa = 6.04 TDD1823 pKa = 3.71 GVRR1826 pKa = 11.84 HH1827 pKa = 5.99 GATVRR1832 pKa = 11.84 LTLNGEE1838 pKa = 4.03 HH1839 pKa = 5.76 YY1840 pKa = 7.81 TAKK1843 pKa = 10.3 VGKK1846 pKa = 9.86 DD1847 pKa = 3.57 GSWSAKK1853 pKa = 9.74 VPAADD1858 pKa = 3.35 VAALEE1863 pKa = 4.61 EE1864 pKa = 5.01 GSATLTACVKK1874 pKa = 9.85 SACGQMLYY1882 pKa = 10.91 AKK1884 pKa = 10.34 AVLTVDD1890 pKa = 3.31 TRR1892 pKa = 11.84 APEE1895 pKa = 4.13 LALLPVTGDD1904 pKa = 3.56 NLISLQEE1911 pKa = 4.04 AQAGFALTGSAGIAAAGLLVLVVLNGVEE1939 pKa = 4.19 YY1940 pKa = 10.2 QGKK1943 pKa = 8.51 VQASGDD1949 pKa = 3.47 WTVAIPAGTLADD1961 pKa = 4.17 QPAGDD1966 pKa = 4.01 YY1967 pKa = 10.28 PLTVSLTDD1975 pKa = 3.26 TAGNLTSLTSVVTLEE1990 pKa = 4.3 AAPDD1994 pKa = 3.56 ADD1996 pKa = 3.75 ATEE1999 pKa = 4.41 TAALTVTSQTVAASADD2015 pKa = 3.7 STLTSALAVPDD2026 pKa = 4.19 TATDD2030 pKa = 3.71 TADD2033 pKa = 2.94 GTYY2036 pKa = 10.45 AIGGQTLTLTEE2047 pKa = 4.37 SGGEE2051 pKa = 3.91 ALGGSGNDD2059 pKa = 3.53 TILLNTLDD2067 pKa = 4.37 FLHH2070 pKa = 7.2 IDD2072 pKa = 4.04 GGSGTDD2078 pKa = 3.35 TLLLAGSDD2086 pKa = 3.36 QHH2088 pKa = 8.28 LDD2090 pKa = 3.34 LTTLGLKK2097 pKa = 9.77 IEE2099 pKa = 4.97 NIDD2102 pKa = 4.76 IFDD2105 pKa = 4.87 LGNGSNSLTLGLHH2118 pKa = 5.09 EE2119 pKa = 5.32 AEE2121 pKa = 4.65 TVRR2124 pKa = 11.84 DD2125 pKa = 3.94 TPEE2128 pKa = 3.39 EE2129 pKa = 4.02 SLFIRR2134 pKa = 11.84 GQGGSQLTLAGDD2146 pKa = 4.13 NTWEE2150 pKa = 3.97 TSGQRR2155 pKa = 11.84 EE2156 pKa = 4.21 VGGLLFDD2163 pKa = 4.44 VYY2165 pKa = 10.61 HH2166 pKa = 5.88 ATGLEE2171 pKa = 4.21 SADD2174 pKa = 4.35 LLVQQGILVQQGG2186 pKa = 2.84
Molecular weight: 225.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.567
IPC_protein 3.617
Toseland 3.389
ProMoST 3.783
Dawson 3.617
Bjellqvist 3.77
Wikipedia 3.554
Rodwell 3.439
Grimsley 3.287
Solomon 3.617
Lehninger 3.567
Nozaki 3.719
DTASelect 3.986
Thurlkill 3.439
EMBOSS 3.554
Sillero 3.745
Patrickios 1.354
IPC_peptide 3.605
IPC2_peptide 3.719
IPC2.peptide.svr19 3.694
Protein with the highest isoelectric point:
>tr|A0A2N5ELK9|A0A2N5ELK9_9GAMM FMN-binding protein MioC OS=Chimaeribacter arupi OX=2060066 GN=CYR34_13685 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.25 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSVSKK46 pKa = 10.99
Molecular weight: 5.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4159
0
4159
1358501
15
6646
326.6
35.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.823 ± 0.055
1.017 ± 0.014
5.151 ± 0.038
5.344 ± 0.037
3.744 ± 0.028
7.739 ± 0.037
2.388 ± 0.022
5.206 ± 0.035
3.541 ± 0.038
11.169 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.625 ± 0.024
3.406 ± 0.031
4.924 ± 0.03
4.633 ± 0.037
5.901 ± 0.047
5.557 ± 0.032
5.504 ± 0.065
7.129 ± 0.035
1.416 ± 0.016
2.782 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here