Pasteurella phage Pm86

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4PZT5|A0A2Z4PZT5_9CAUD DNA methyltransferase OS=Pasteurella phage Pm86 OX=2201742 PE=4 SV=1
MM1 pKa = 6.93TQLTANQEE9 pKa = 3.83KK10 pKa = 10.48AIRR13 pKa = 11.84AFKK16 pKa = 10.58FSFTNQGVFNVDD28 pKa = 2.91QLVAYY33 pKa = 9.26FEE35 pKa = 5.15AEE37 pKa = 4.02GDD39 pKa = 3.73DD40 pKa = 4.2TEE42 pKa = 4.68TKK44 pKa = 10.29TFLATAYY51 pKa = 9.53ALNGAYY57 pKa = 9.88EE58 pKa = 4.12SGYY61 pKa = 10.68FDD63 pKa = 4.05IDD65 pKa = 3.3NAEE68 pKa = 4.22AQLEE72 pKa = 4.13ALNGYY77 pKa = 9.12GVDD80 pKa = 4.91GFDD83 pKa = 3.68FDD85 pKa = 5.5KK86 pKa = 11.24ALEE89 pKa = 4.22LCEE92 pKa = 4.41VFFDD96 pKa = 4.35LVEE99 pKa = 4.53KK100 pKa = 10.68GLYY103 pKa = 8.91EE104 pKa = 4.3VEE106 pKa = 4.1

Molecular weight: 11.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4Q085|A0A2Z4Q085_9CAUD Uncharacterized protein OS=Pasteurella phage Pm86 OX=2201742 PE=4 SV=1
MM1 pKa = 7.29VNVKK5 pKa = 10.03FYY7 pKa = 11.4GSLRR11 pKa = 11.84QFGTSFKK18 pKa = 10.94LDD20 pKa = 3.41AEE22 pKa = 4.29NTAEE26 pKa = 4.83IIRR29 pKa = 11.84ALTSQIPKK37 pKa = 9.89LRR39 pKa = 11.84EE40 pKa = 4.15FIQKK44 pKa = 10.32GYY46 pKa = 8.05FTVRR50 pKa = 11.84IAKK53 pKa = 9.72EE54 pKa = 4.1YY55 pKa = 10.0IDD57 pKa = 4.07NRR59 pKa = 11.84YY60 pKa = 9.44LEE62 pKa = 4.7KK63 pKa = 11.05GLFYY67 pKa = 10.85KK68 pKa = 10.58LKK70 pKa = 10.66EE71 pKa = 3.91GMTVHH76 pKa = 6.26FTPVLKK82 pKa = 10.41GSKK85 pKa = 9.71RR86 pKa = 11.84GGVFQTILGAALVGAAFLLGPVGFGLLSANSAWVVGGLGASLMLGGVAQLLTRR139 pKa = 11.84QPQMPAINEE148 pKa = 4.18KK149 pKa = 10.11EE150 pKa = 4.37KK151 pKa = 10.91KK152 pKa = 10.03QSTSFSNLSNMAAQGRR168 pKa = 11.84MVPLAYY174 pKa = 10.22GRR176 pKa = 11.84IRR178 pKa = 11.84CGSLVISQGVQTLDD192 pKa = 3.27VNIVEE197 pKa = 4.85KK198 pKa = 10.81NQNTGFSKK206 pKa = 10.96GG207 pKa = 3.38

Molecular weight: 22.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

16028

59

1113

197.9

22.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.031 ± 0.297

1.254 ± 0.141

5.334 ± 0.176

7.861 ± 0.327

4.243 ± 0.169

6.301 ± 0.291

1.759 ± 0.14

7.138 ± 0.184

8.08 ± 0.288

8.398 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.234 ± 0.111

5.334 ± 0.168

3.219 ± 0.168

4.143 ± 0.2

4.767 ± 0.198

6.744 ± 0.248

5.291 ± 0.283

6.133 ± 0.208

1.341 ± 0.087

3.394 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski