Amino acid dipepetide frequency for Pasteurella phage Pm86

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.995AlaAla: 2.995 ± 0.601
0.811AlaCys: 0.811 ± 0.231
3.432AlaAsp: 3.432 ± 0.433
5.678AlaGlu: 5.678 ± 0.625
3.369AlaPhe: 3.369 ± 0.484
4.243AlaGly: 4.243 ± 0.675
1.123AlaHis: 1.123 ± 0.264
4.867AlaIle: 4.867 ± 0.566
5.927AlaLys: 5.927 ± 0.776
6.551AlaLeu: 6.551 ± 0.724
2.309AlaMet: 2.309 ± 0.377
3.307AlaAsn: 3.307 ± 0.456
1.685AlaPro: 1.685 ± 0.44
3.12AlaGln: 3.12 ± 0.478
3.806AlaArg: 3.806 ± 0.512
4.929AlaSer: 4.929 ± 0.511
3.245AlaThr: 3.245 ± 0.611
4.555AlaVal: 4.555 ± 0.421
1.747AlaTrp: 1.747 ± 0.308
2.621AlaTyr: 2.621 ± 0.402
0.0AlaXaa: 0.0 ± 0.0
Cys
0.749CysAla: 0.749 ± 0.206
0.25CysCys: 0.25 ± 0.159
0.811CysAsp: 0.811 ± 0.2
1.435CysGlu: 1.435 ± 0.335
0.437CysPhe: 0.437 ± 0.164
1.185CysGly: 1.185 ± 0.304
0.187CysHis: 0.187 ± 0.117
0.749CysIle: 0.749 ± 0.208
0.811CysLys: 0.811 ± 0.279
1.061CysLeu: 1.061 ± 0.236
0.125CysMet: 0.125 ± 0.106
0.562CysAsn: 0.562 ± 0.176
0.811CysPro: 0.811 ± 0.248
0.312CysGln: 0.312 ± 0.136
0.686CysArg: 0.686 ± 0.227
0.936CysSer: 0.936 ± 0.27
0.312CysThr: 0.312 ± 0.159
0.686CysVal: 0.686 ± 0.218
0.125CysTrp: 0.125 ± 0.076
0.312CysTyr: 0.312 ± 0.138
0.0CysXaa: 0.0 ± 0.0
Asp
2.558AspAla: 2.558 ± 0.45
0.811AspCys: 0.811 ± 0.277
2.496AspAsp: 2.496 ± 0.44
4.305AspGlu: 4.305 ± 0.639
3.12AspPhe: 3.12 ± 0.485
4.305AspGly: 4.305 ± 0.592
0.874AspHis: 0.874 ± 0.264
4.18AspIle: 4.18 ± 0.521
3.993AspLys: 3.993 ± 0.504
4.929AspLeu: 4.929 ± 0.61
1.31AspMet: 1.31 ± 0.272
2.246AspAsn: 2.246 ± 0.334
1.997AspPro: 1.997 ± 0.367
1.56AspGln: 1.56 ± 0.29
2.184AspArg: 2.184 ± 0.339
2.745AspSer: 2.745 ± 0.415
2.309AspThr: 2.309 ± 0.3
3.744AspVal: 3.744 ± 0.451
0.936AspTrp: 0.936 ± 0.256
2.683AspTyr: 2.683 ± 0.472
0.0AspXaa: 0.0 ± 0.0
Glu
5.304GluAla: 5.304 ± 0.615
0.936GluCys: 0.936 ± 0.262
3.245GluAsp: 3.245 ± 0.501
5.865GluGlu: 5.865 ± 0.81
2.995GluPhe: 2.995 ± 0.478
2.808GluGly: 2.808 ± 0.394
1.56GluHis: 1.56 ± 0.354
7.051GluIle: 7.051 ± 0.613
9.047GluLys: 9.047 ± 0.966
7.55GluLeu: 7.55 ± 0.733
2.184GluMet: 2.184 ± 0.311
3.931GluAsn: 3.931 ± 0.551
1.685GluPro: 1.685 ± 0.379
4.305GluGln: 4.305 ± 0.618
4.056GluArg: 4.056 ± 0.384
4.617GluSer: 4.617 ± 0.529
3.494GluThr: 3.494 ± 0.629
4.368GluVal: 4.368 ± 0.45
1.248GluTrp: 1.248 ± 0.282
2.371GluTyr: 2.371 ± 0.382
0.0GluXaa: 0.0 ± 0.0
Phe
2.808PheAla: 2.808 ± 0.483
0.562PheCys: 0.562 ± 0.221
3.12PheAsp: 3.12 ± 0.525
3.744PheGlu: 3.744 ± 0.46
1.435PhePhe: 1.435 ± 0.29
3.12PheGly: 3.12 ± 0.409
0.811PheHis: 0.811 ± 0.204
2.246PheIle: 2.246 ± 0.357
3.245PheLys: 3.245 ± 0.313
2.933PheLeu: 2.933 ± 0.428
0.624PheMet: 0.624 ± 0.153
2.683PheAsn: 2.683 ± 0.382
1.123PhePro: 1.123 ± 0.278
1.248PheGln: 1.248 ± 0.276
1.872PheArg: 1.872 ± 0.359
2.87PheSer: 2.87 ± 0.375
2.558PheThr: 2.558 ± 0.455
2.808PheVal: 2.808 ± 0.478
0.686PheTrp: 0.686 ± 0.199
1.934PheTyr: 1.934 ± 0.383
0.0PheXaa: 0.0 ± 0.0
Gly
4.18GlyAla: 4.18 ± 0.644
0.749GlyCys: 0.749 ± 0.212
3.681GlyAsp: 3.681 ± 0.568
3.993GlyGlu: 3.993 ± 0.511
3.556GlyPhe: 3.556 ± 0.453
4.68GlyGly: 4.68 ± 0.628
0.936GlyHis: 0.936 ± 0.245
4.867GlyIle: 4.867 ± 0.503
4.867GlyLys: 4.867 ± 0.463
5.304GlyLeu: 5.304 ± 0.584
1.934GlyMet: 1.934 ± 0.308
4.368GlyAsn: 4.368 ± 0.721
1.123GlyPro: 1.123 ± 0.653
2.87GlyGln: 2.87 ± 0.577
2.433GlyArg: 2.433 ± 0.387
2.995GlySer: 2.995 ± 0.479
2.496GlyThr: 2.496 ± 0.391
4.992GlyVal: 4.992 ± 0.581
0.998GlyTrp: 0.998 ± 0.252
1.997GlyTyr: 1.997 ± 0.385
0.0GlyXaa: 0.0 ± 0.0
His
0.998HisAla: 0.998 ± 0.244
0.25HisCys: 0.25 ± 0.112
0.811HisAsp: 0.811 ± 0.28
0.998HisGlu: 0.998 ± 0.237
0.874HisPhe: 0.874 ± 0.221
1.31HisGly: 1.31 ± 0.327
0.437HisHis: 0.437 ± 0.171
1.622HisIle: 1.622 ± 0.307
1.248HisLys: 1.248 ± 0.266
1.56HisLeu: 1.56 ± 0.269
0.125HisMet: 0.125 ± 0.089
0.624HisAsn: 0.624 ± 0.192
0.936HisPro: 0.936 ± 0.265
0.374HisGln: 0.374 ± 0.149
0.874HisArg: 0.874 ± 0.228
1.685HisSer: 1.685 ± 0.332
0.874HisThr: 0.874 ± 0.278
0.749HisVal: 0.749 ± 0.206
0.374HisTrp: 0.374 ± 0.177
0.874HisTyr: 0.874 ± 0.299
0.0HisXaa: 0.0 ± 0.0
Ile
5.553IleAla: 5.553 ± 0.598
0.686IleCys: 0.686 ± 0.154
4.68IleAsp: 4.68 ± 0.607
6.177IleGlu: 6.177 ± 0.563
1.934IlePhe: 1.934 ± 0.373
3.307IleGly: 3.307 ± 0.441
1.061IleHis: 1.061 ± 0.25
5.054IleIle: 5.054 ± 0.515
6.676IleLys: 6.676 ± 0.64
4.867IleLeu: 4.867 ± 0.6
0.686IleMet: 0.686 ± 0.235
4.243IleAsn: 4.243 ± 0.422
3.182IlePro: 3.182 ± 0.48
2.558IleGln: 2.558 ± 0.44
3.245IleArg: 3.245 ± 0.605
6.926IleSer: 6.926 ± 0.742
3.432IleThr: 3.432 ± 0.466
4.305IleVal: 4.305 ± 0.493
0.562IleTrp: 0.562 ± 0.181
2.246IleTyr: 2.246 ± 0.321
0.0IleXaa: 0.0 ± 0.0
Lys
6.801LysAla: 6.801 ± 0.899
1.373LysCys: 1.373 ± 0.306
3.432LysAsp: 3.432 ± 0.523
6.052LysGlu: 6.052 ± 0.671
3.681LysPhe: 3.681 ± 0.498
4.43LysGly: 4.43 ± 0.493
1.685LysHis: 1.685 ± 0.341
5.304LysIle: 5.304 ± 0.557
5.865LysLys: 5.865 ± 0.936
6.177LysLeu: 6.177 ± 0.541
1.809LysMet: 1.809 ± 0.33
4.68LysAsn: 4.68 ± 0.52
2.309LysPro: 2.309 ± 0.469
4.118LysGln: 4.118 ± 0.476
3.744LysArg: 3.744 ± 0.516
5.491LysSer: 5.491 ± 0.709
5.428LysThr: 5.428 ± 0.564
4.867LysVal: 4.867 ± 0.53
0.874LysTrp: 0.874 ± 0.231
2.683LysTyr: 2.683 ± 0.403
0.0LysXaa: 0.0 ± 0.0
Leu
7.051LeuAla: 7.051 ± 0.689
0.936LeuCys: 0.936 ± 0.269
5.428LeuAsp: 5.428 ± 0.494
7.175LeuGlu: 7.175 ± 0.656
2.246LeuPhe: 2.246 ± 0.308
4.804LeuGly: 4.804 ± 0.636
1.248LeuHis: 1.248 ± 0.299
5.927LeuIle: 5.927 ± 0.717
6.926LeuLys: 6.926 ± 0.738
5.803LeuLeu: 5.803 ± 0.782
1.622LeuMet: 1.622 ± 0.334
5.054LeuAsn: 5.054 ± 0.754
2.745LeuPro: 2.745 ± 0.423
3.12LeuGln: 3.12 ± 0.5
4.368LeuArg: 4.368 ± 0.603
6.801LeuSer: 6.801 ± 0.548
4.555LeuThr: 4.555 ± 0.641
4.617LeuVal: 4.617 ± 0.496
0.936LeuTrp: 0.936 ± 0.205
2.621LeuTyr: 2.621 ± 0.424
0.0LeuXaa: 0.0 ± 0.0
Met
1.56MetAla: 1.56 ± 0.321
0.312MetCys: 0.312 ± 0.14
1.248MetAsp: 1.248 ± 0.288
1.622MetGlu: 1.622 ± 0.32
0.811MetPhe: 0.811 ± 0.204
0.686MetGly: 0.686 ± 0.204
0.312MetHis: 0.312 ± 0.133
1.373MetIle: 1.373 ± 0.384
2.371MetLys: 2.371 ± 0.413
2.059MetLeu: 2.059 ± 0.317
0.062MetMet: 0.062 ± 0.059
1.31MetAsn: 1.31 ± 0.226
1.248MetPro: 1.248 ± 0.301
0.562MetGln: 0.562 ± 0.172
1.497MetArg: 1.497 ± 0.243
1.31MetSer: 1.31 ± 0.25
2.059MetThr: 2.059 ± 0.328
1.248MetVal: 1.248 ± 0.296
0.125MetTrp: 0.125 ± 0.089
0.562MetTyr: 0.562 ± 0.168
0.0MetXaa: 0.0 ± 0.0
Asn
4.555AsnAla: 4.555 ± 0.425
0.374AsnCys: 0.374 ± 0.165
2.246AsnAsp: 2.246 ± 0.303
3.619AsnGlu: 3.619 ± 0.452
2.309AsnPhe: 2.309 ± 0.39
5.428AsnGly: 5.428 ± 0.562
0.936AsnHis: 0.936 ± 0.324
3.307AsnIle: 3.307 ± 0.476
4.617AsnLys: 4.617 ± 0.583
4.118AsnLeu: 4.118 ± 0.537
1.061AsnMet: 1.061 ± 0.307
2.87AsnAsn: 2.87 ± 0.458
1.872AsnPro: 1.872 ± 0.324
1.872AsnGln: 1.872 ± 0.365
2.558AsnArg: 2.558 ± 0.357
3.432AsnSer: 3.432 ± 0.522
3.307AsnThr: 3.307 ± 0.48
3.12AsnVal: 3.12 ± 0.523
0.437AsnTrp: 0.437 ± 0.184
1.31AsnTyr: 1.31 ± 0.277
0.0AsnXaa: 0.0 ± 0.0
Pro
1.872ProAla: 1.872 ± 0.283
0.312ProCys: 0.312 ± 0.186
1.809ProAsp: 1.809 ± 0.331
2.87ProGlu: 2.87 ± 0.499
1.061ProPhe: 1.061 ± 0.239
1.061ProGly: 1.061 ± 0.481
0.811ProHis: 0.811 ± 0.221
1.934ProIle: 1.934 ± 0.307
2.246ProLys: 2.246 ± 0.392
2.433ProLeu: 2.433 ± 0.352
0.624ProMet: 0.624 ± 0.229
2.246ProAsn: 2.246 ± 0.374
1.497ProPro: 1.497 ± 0.406
1.685ProGln: 1.685 ± 0.313
1.685ProArg: 1.685 ± 0.35
2.496ProSer: 2.496 ± 0.523
1.622ProThr: 1.622 ± 0.435
2.433ProVal: 2.433 ± 0.373
0.374ProTrp: 0.374 ± 0.18
1.123ProTyr: 1.123 ± 0.229
0.0ProXaa: 0.0 ± 0.0
Gln
3.432GlnAla: 3.432 ± 0.588
0.811GlnCys: 0.811 ± 0.233
2.309GlnAsp: 2.309 ± 0.426
2.496GlnGlu: 2.496 ± 0.41
1.934GlnPhe: 1.934 ± 0.291
2.933GlnGly: 2.933 ± 0.49
0.749GlnHis: 0.749 ± 0.198
3.12GlnIle: 3.12 ± 0.457
2.995GlnLys: 2.995 ± 0.449
3.307GlnLeu: 3.307 ± 0.534
1.185GlnMet: 1.185 ± 0.243
2.433GlnAsn: 2.433 ± 0.335
1.185GlnPro: 1.185 ± 0.251
1.56GlnGln: 1.56 ± 0.351
1.809GlnArg: 1.809 ± 0.335
3.057GlnSer: 3.057 ± 0.383
2.059GlnThr: 2.059 ± 0.422
2.371GlnVal: 2.371 ± 0.383
0.312GlnTrp: 0.312 ± 0.12
1.373GlnTyr: 1.373 ± 0.318
0.0GlnXaa: 0.0 ± 0.0
Arg
2.621ArgAla: 2.621 ± 0.406
0.499ArgCys: 0.499 ± 0.166
2.683ArgAsp: 2.683 ± 0.512
4.243ArgGlu: 4.243 ± 0.56
1.934ArgPhe: 1.934 ± 0.352
2.558ArgGly: 2.558 ± 0.311
0.811ArgHis: 0.811 ± 0.245
4.118ArgIle: 4.118 ± 0.515
4.492ArgLys: 4.492 ± 0.547
4.929ArgLeu: 4.929 ± 0.543
1.56ArgMet: 1.56 ± 0.291
2.496ArgAsn: 2.496 ± 0.403
1.123ArgPro: 1.123 ± 0.27
2.121ArgGln: 2.121 ± 0.379
1.934ArgArg: 1.934 ± 0.364
2.309ArgSer: 2.309 ± 0.362
2.121ArgThr: 2.121 ± 0.367
2.558ArgVal: 2.558 ± 0.367
0.749ArgTrp: 0.749 ± 0.219
1.809ArgTyr: 1.809 ± 0.297
0.0ArgXaa: 0.0 ± 0.0
Ser
4.867SerAla: 4.867 ± 0.716
0.686SerCys: 0.686 ± 0.201
3.494SerAsp: 3.494 ± 0.582
6.427SerGlu: 6.427 ± 0.549
3.432SerPhe: 3.432 ± 0.544
5.241SerGly: 5.241 ± 0.72
1.123SerHis: 1.123 ± 0.246
4.18SerIle: 4.18 ± 0.565
5.616SerLys: 5.616 ± 0.596
4.804SerLeu: 4.804 ± 0.484
1.809SerMet: 1.809 ± 0.289
2.246SerAsn: 2.246 ± 0.325
1.809SerPro: 1.809 ± 0.343
3.432SerGln: 3.432 ± 0.463
3.619SerArg: 3.619 ± 0.472
3.868SerSer: 3.868 ± 0.595
3.556SerThr: 3.556 ± 0.564
4.43SerVal: 4.43 ± 0.411
0.749SerTrp: 0.749 ± 0.233
2.059SerTyr: 2.059 ± 0.356
0.0SerXaa: 0.0 ± 0.0
Thr
4.43ThrAla: 4.43 ± 0.59
0.312ThrCys: 0.312 ± 0.15
2.059ThrAsp: 2.059 ± 0.308
4.305ThrGlu: 4.305 ± 0.549
2.059ThrPhe: 2.059 ± 0.431
4.305ThrGly: 4.305 ± 0.507
1.061ThrHis: 1.061 ± 0.28
3.619ThrIle: 3.619 ± 0.479
2.683ThrLys: 2.683 ± 0.448
5.803ThrLeu: 5.803 ± 0.494
0.874ThrMet: 0.874 ± 0.228
2.184ThrAsn: 2.184 ± 0.354
2.371ThrPro: 2.371 ± 0.415
2.558ThrGln: 2.558 ± 0.345
2.184ThrArg: 2.184 ± 0.335
3.369ThrSer: 3.369 ± 0.623
2.995ThrThr: 2.995 ± 0.489
3.057ThrVal: 3.057 ± 0.526
0.562ThrTrp: 0.562 ± 0.202
2.121ThrTyr: 2.121 ± 0.449
0.0ThrXaa: 0.0 ± 0.0
Val
5.116ValAla: 5.116 ± 0.587
0.936ValCys: 0.936 ± 0.291
3.744ValAsp: 3.744 ± 0.493
4.368ValGlu: 4.368 ± 0.575
3.12ValPhe: 3.12 ± 0.409
3.245ValGly: 3.245 ± 0.476
1.123ValHis: 1.123 ± 0.36
4.555ValIle: 4.555 ± 0.544
4.368ValLys: 4.368 ± 0.446
5.366ValLeu: 5.366 ± 0.499
1.56ValMet: 1.56 ± 0.347
3.993ValAsn: 3.993 ± 0.422
1.872ValPro: 1.872 ± 0.296
1.56ValGln: 1.56 ± 0.313
2.496ValArg: 2.496 ± 0.41
4.18ValSer: 4.18 ± 0.638
3.432ValThr: 3.432 ± 0.66
3.494ValVal: 3.494 ± 0.486
0.998ValTrp: 0.998 ± 0.272
1.809ValTyr: 1.809 ± 0.375
0.0ValXaa: 0.0 ± 0.0
Trp
0.624TrpAla: 0.624 ± 0.194
0.374TrpCys: 0.374 ± 0.139
0.686TrpAsp: 0.686 ± 0.2
0.936TrpGlu: 0.936 ± 0.228
0.749TrpPhe: 0.749 ± 0.218
1.061TrpGly: 1.061 ± 0.229
0.187TrpHis: 0.187 ± 0.096
1.061TrpIle: 1.061 ± 0.223
0.749TrpLys: 0.749 ± 0.228
1.435TrpLeu: 1.435 ± 0.255
0.0TrpMet: 0.0 ± 0.0
0.686TrpAsn: 0.686 ± 0.217
0.125TrpPro: 0.125 ± 0.089
0.998TrpGln: 0.998 ± 0.27
0.624TrpArg: 0.624 ± 0.204
0.749TrpSer: 0.749 ± 0.194
1.123TrpThr: 1.123 ± 0.233
0.811TrpVal: 0.811 ± 0.213
0.187TrpTrp: 0.187 ± 0.102
0.25TrpTyr: 0.25 ± 0.116
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.246TyrAla: 2.246 ± 0.348
0.562TyrCys: 0.562 ± 0.175
1.934TyrAsp: 1.934 ± 0.375
2.309TyrGlu: 2.309 ± 0.346
1.373TyrPhe: 1.373 ± 0.29
2.309TyrGly: 2.309 ± 0.405
0.562TyrHis: 0.562 ± 0.214
2.246TyrIle: 2.246 ± 0.338
2.059TyrLys: 2.059 ± 0.338
2.995TyrLeu: 2.995 ± 0.479
0.811TyrMet: 0.811 ± 0.244
1.185TyrAsn: 1.185 ± 0.284
1.497TyrPro: 1.497 ± 0.429
1.497TyrGln: 1.497 ± 0.316
1.997TyrArg: 1.997 ± 0.361
2.683TyrSer: 2.683 ± 0.419
1.934TyrThr: 1.934 ± 0.325
2.121TyrVal: 2.121 ± 0.34
0.437TyrTrp: 0.437 ± 0.164
1.185TyrTyr: 1.185 ± 0.217
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 81 proteins (16028 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski