Brachybacterium sp. SGAir0954

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Dermabacteraceae; Brachybacterium; unclassified Brachybacterium

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2953 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8TH95|A0A4P8TH95_9MICO Oxidoreductase OS=Brachybacterium sp. SGAir0954 OX=2571029 GN=C1N80_06695 PE=4 SV=1
MM1 pKa = 7.65SSTLPASRR9 pKa = 11.84RR10 pKa = 11.84PLARR14 pKa = 11.84LGVLAAAALLAVTGCTPGGGSDD36 pKa = 4.23GADD39 pKa = 3.37ASDD42 pKa = 3.69GGADD46 pKa = 3.37QGAASTGTAAAEE58 pKa = 3.96LADD61 pKa = 4.91LGTSHH66 pKa = 7.43AADD69 pKa = 4.51LDD71 pKa = 3.79VDD73 pKa = 4.19PASDD77 pKa = 3.4PAYY80 pKa = 10.13AQYY83 pKa = 11.27YY84 pKa = 8.81DD85 pKa = 3.8QQIDD89 pKa = 2.99WGACEE94 pKa = 4.71DD95 pKa = 3.67VSGSVGLEE103 pKa = 3.96CGTLTVPLAWDD114 pKa = 4.25DD115 pKa = 4.01PSAGDD120 pKa = 3.67VEE122 pKa = 4.65LAVARR127 pKa = 11.84QGATVDD133 pKa = 3.42AKK135 pKa = 11.17GSLVVNPGGPGGSGVDD151 pKa = 3.48MAAQADD157 pKa = 4.35AYY159 pKa = 10.83FSADD163 pKa = 2.79VMASYY168 pKa = 11.06DD169 pKa = 3.58VVGFDD174 pKa = 3.51PRR176 pKa = 11.84GVARR180 pKa = 11.84SQGIDD185 pKa = 3.45CLTDD189 pKa = 3.24EE190 pKa = 6.09DD191 pKa = 5.03LDD193 pKa = 3.91QMRR196 pKa = 11.84ADD198 pKa = 4.05SFDD201 pKa = 4.12LLTEE205 pKa = 4.31GGEE208 pKa = 4.34RR209 pKa = 11.84AQHH212 pKa = 5.68PWVEE216 pKa = 4.39RR217 pKa = 11.84LAEE220 pKa = 4.32GCEE223 pKa = 4.05DD224 pKa = 3.92DD225 pKa = 4.76PGSAEE230 pKa = 4.33LLPYY234 pKa = 10.54LDD236 pKa = 4.1TASSARR242 pKa = 11.84DD243 pKa = 3.44LDD245 pKa = 3.85VLRR248 pKa = 11.84AAAGSEE254 pKa = 3.58QLDD257 pKa = 3.9YY258 pKa = 11.17FGYY261 pKa = 10.4SYY263 pKa = 10.69GTFLGSTYY271 pKa = 10.96AGLYY275 pKa = 7.78PEE277 pKa = 4.36RR278 pKa = 11.84VGRR281 pKa = 11.84MVLDD285 pKa = 3.99GAVDD289 pKa = 3.69PTLTADD295 pKa = 4.69DD296 pKa = 5.32FSAGQAGGFEE306 pKa = 4.16QATRR310 pKa = 11.84TFLEE314 pKa = 4.64DD315 pKa = 3.49CLKK318 pKa = 10.59QGEE321 pKa = 4.89GEE323 pKa = 4.47CPFTGDD329 pKa = 2.79VDD331 pKa = 4.21AAAQQLLDD339 pKa = 4.39LFEE342 pKa = 4.94KK343 pKa = 10.56AQSDD347 pKa = 4.31PLPTDD352 pKa = 3.87DD353 pKa = 5.3PDD355 pKa = 4.4RR356 pKa = 11.84PLTGALARR364 pKa = 11.84SAVLMLMYY372 pKa = 10.48DD373 pKa = 3.7DD374 pKa = 6.06SSWQYY379 pKa = 10.58GRR381 pKa = 11.84EE382 pKa = 4.24GIAAALDD389 pKa = 3.69GDD391 pKa = 4.3GQILLNIADD400 pKa = 3.66QSASRR405 pKa = 11.84NRR407 pKa = 11.84DD408 pKa = 2.43GSYY411 pKa = 10.51SGNGNEE417 pKa = 5.63AITAVNCLDD426 pKa = 3.76RR427 pKa = 11.84PGLASEE433 pKa = 4.48EE434 pKa = 4.41EE435 pKa = 4.45EE436 pKa = 3.99TALAHH441 pKa = 6.57QLAKK445 pKa = 10.49DD446 pKa = 3.73DD447 pKa = 4.53PVWGSSFSYY456 pKa = 10.72AATTCGYY463 pKa = 9.42WPEE466 pKa = 4.14KK467 pKa = 9.61PVRR470 pKa = 11.84EE471 pKa = 4.28PAPVTADD478 pKa = 3.1GAAPIVVIGTTGDD491 pKa = 3.39PATPYY496 pKa = 9.2TWAEE500 pKa = 3.98SLADD504 pKa = 3.72QLSSGVLLTFEE515 pKa = 5.22GNGHH519 pKa = 4.96TAYY522 pKa = 10.14GRR524 pKa = 11.84SGGCIEE530 pKa = 4.49EE531 pKa = 4.2QVDD534 pKa = 3.8AYY536 pKa = 10.85LLEE539 pKa = 4.55GTTPAEE545 pKa = 4.45GARR548 pKa = 11.84CC549 pKa = 3.48

Molecular weight: 56.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8THF7|A0A4P8THF7_9MICO 1 4-dihydroxy-2-naphthoate octaprenyltransferase OS=Brachybacterium sp. SGAir0954 OX=2571029 GN=menA PE=3 SV=1
MM1 pKa = 7.28GSVVKK6 pKa = 10.44KK7 pKa = 9.48RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.32RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.79HH17 pKa = 5.67RR18 pKa = 11.84KK19 pKa = 8.31LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2953

0

2953

1012604

32

2236

342.9

36.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.721 ± 0.07

0.511 ± 0.01

6.137 ± 0.037

6.323 ± 0.052

2.612 ± 0.031

9.442 ± 0.036

2.194 ± 0.023

3.76 ± 0.031

1.619 ± 0.032

10.751 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.817 ± 0.018

1.475 ± 0.021

5.811 ± 0.035

2.737 ± 0.025

7.714 ± 0.057

5.422 ± 0.035

6.111 ± 0.038

8.677 ± 0.044

1.41 ± 0.02

1.757 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski