Amino acid dipepetide frequency for Brachybacterium sp. SGAir0954

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.554AlaAla: 21.554 ± 0.246	0.714AlaCys: 0.714 ± 0.027	8.109AlaAsp: 8.109 ± 0.123	9.287AlaGlu: 9.287 ± 0.126	3.297AlaPhe: 3.297 ± 0.061	13.217AlaGly: 13.217 ± 0.129	2.712AlaHis: 2.712 ± 0.063	5.036AlaIle: 5.036 ± 0.074	2.203AlaLys: 2.203 ± 0.065	15.566AlaLeu: 15.566 ± 0.151	2.667AlaMet: 2.667 ± 0.05	1.666AlaAsn: 1.666 ± 0.043	7.74AlaPro: 7.74 ± 0.127	3.964AlaGln: 3.964 ± 0.064	10.015AlaArg: 10.015 ± 0.108	6.792AlaSer: 6.792 ± 0.092	7.071AlaThr: 7.071 ± 0.088	11.689AlaVal: 11.689 ± 0.117	1.835AlaTrp: 1.835 ± 0.052	2.075AlaTyr: 2.075 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	0.682CysAla: 0.682 ± 0.025	0.046CysCys: 0.046 ± 0.007	0.27CysAsp: 0.27 ± 0.017	0.323CysGlu: 0.323 ± 0.018	0.152CysPhe: 0.152 ± 0.014	0.627CysGly: 0.627 ± 0.026	0.122CysHis: 0.122 ± 0.011	0.158CysIle: 0.158 ± 0.013	0.047CysLys: 0.047 ± 0.008	0.465CysLeu: 0.465 ± 0.023	0.072CysMet: 0.072 ± 0.007	0.092CysAsn: 0.092 ± 0.009	0.325CysPro: 0.325 ± 0.021	0.104CysGln: 0.104 ± 0.01	0.364CysArg: 0.364 ± 0.019	0.338CysSer: 0.338 ± 0.016	0.388CysThr: 0.388 ± 0.023	0.359CysVal: 0.359 ± 0.02	0.066CysTrp: 0.066 ± 0.008	0.113CysTyr: 0.113 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	8.75AspAla: 8.75 ± 0.126	0.245AspCys: 0.245 ± 0.016	3.449AspAsp: 3.449 ± 0.073	4.198AspGlu: 4.198 ± 0.062	1.368AspPhe: 1.368 ± 0.037	6.648AspGly: 6.648 ± 0.117	1.492AspHis: 1.492 ± 0.039	1.567AspIle: 1.567 ± 0.05	0.785AspLys: 0.785 ± 0.027	7.374AspLeu: 7.374 ± 0.087	0.895AspMet: 0.895 ± 0.033	0.669AspAsn: 0.669 ± 0.026	5.182AspPro: 5.182 ± 0.079	1.58AspGln: 1.58 ± 0.044	4.498AspArg: 4.498 ± 0.081	2.559AspSer: 2.559 ± 0.058	2.732AspThr: 2.732 ± 0.054	5.397AspVal: 5.397 ± 0.079	0.894AspTrp: 0.894 ± 0.032	1.087AspTyr: 1.087 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	8.025GluAla: 8.025 ± 0.109	0.231GluCys: 0.231 ± 0.016	4.63GluAsp: 4.63 ± 0.092	4.709GluGlu: 4.709 ± 0.081	1.354GluPhe: 1.354 ± 0.038	5.226GluGly: 5.226 ± 0.077	1.989GluHis: 1.989 ± 0.05	2.863GluIle: 2.863 ± 0.055	1.479GluLys: 1.479 ± 0.041	6.738GluLeu: 6.738 ± 0.092	0.927GluMet: 0.927 ± 0.031	1.13GluAsn: 1.13 ± 0.035	2.979GluPro: 2.979 ± 0.063	2.828GluGln: 2.828 ± 0.056	5.611GluArg: 5.611 ± 0.092	2.733GluSer: 2.733 ± 0.055	2.858GluThr: 2.858 ± 0.054	5.318GluVal: 5.318 ± 0.077	0.629GluTrp: 0.629 ± 0.025	0.97GluTyr: 0.97 ± 0.033	0.0GluXaa: 0.0 ± 0.0
Phe	3.372PheAla: 3.372 ± 0.066	0.164PheCys: 0.164 ± 0.013	1.8PheAsp: 1.8 ± 0.053	1.514PheGlu: 1.514 ± 0.039	0.841PhePhe: 0.841 ± 0.039	2.703PheGly: 2.703 ± 0.06	0.562PheHis: 0.562 ± 0.025	0.739PheIle: 0.739 ± 0.025	0.305PheLys: 0.305 ± 0.02	2.74PheLeu: 2.74 ± 0.061	0.428PheMet: 0.428 ± 0.024	0.43PheAsn: 0.43 ± 0.024	1.356PhePro: 1.356 ± 0.038	0.669PheGln: 0.669 ± 0.027	1.587PheArg: 1.587 ± 0.043	1.607PheSer: 1.607 ± 0.046	1.997PheThr: 1.997 ± 0.047	2.304PheVal: 2.304 ± 0.05	0.435PheTrp: 0.435 ± 0.021	0.565PheTyr: 0.565 ± 0.025	0.0PheXaa: 0.0 ± 0.0
Gly	12.442GlyAla: 12.442 ± 0.143	0.551GlyCys: 0.551 ± 0.027	5.213GlyAsp: 5.213 ± 0.077	6.04GlyGlu: 6.04 ± 0.093	2.722GlyPhe: 2.722 ± 0.055	8.691GlyGly: 8.691 ± 0.129	2.054GlyHis: 2.054 ± 0.042	4.132GlyIle: 4.132 ± 0.062	1.947GlyLys: 1.947 ± 0.055	9.179GlyLeu: 9.179 ± 0.102	2.107GlyMet: 2.107 ± 0.045	1.431GlyAsn: 1.431 ± 0.044	4.676GlyPro: 4.676 ± 0.072	2.454GlyGln: 2.454 ± 0.053	7.26GlyArg: 7.26 ± 0.103	5.645GlySer: 5.645 ± 0.076	6.729GlyThr: 6.729 ± 0.088	7.652GlyVal: 7.652 ± 0.084	1.482GlyTrp: 1.482 ± 0.039	2.013GlyTyr: 2.013 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	2.558HisAla: 2.558 ± 0.053	0.116HisCys: 0.116 ± 0.011	1.413HisAsp: 1.413 ± 0.042	1.502HisGlu: 1.502 ± 0.036	0.471HisPhe: 0.471 ± 0.021	2.357HisGly: 2.357 ± 0.049	0.674HisHis: 0.674 ± 0.026	0.509HisIle: 0.509 ± 0.023	0.243HisLys: 0.243 ± 0.016	2.743HisLeu: 2.743 ± 0.057	0.285HisMet: 0.285 ± 0.016	0.256HisAsn: 0.256 ± 0.016	1.666HisPro: 1.666 ± 0.047	0.565HisGln: 0.565 ± 0.024	2.118HisArg: 2.118 ± 0.046	0.878HisSer: 0.878 ± 0.029	1.025HisThr: 1.025 ± 0.027	1.888HisVal: 1.888 ± 0.045	0.288HisTrp: 0.288 ± 0.018	0.383HisTyr: 0.383 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	5.931IleAla: 5.931 ± 0.08	0.201IleCys: 0.201 ± 0.014	2.909IleAsp: 2.909 ± 0.05	2.34IleGlu: 2.34 ± 0.051	0.833IlePhe: 0.833 ± 0.032	4.28IleGly: 4.28 ± 0.076	0.665IleHis: 0.665 ± 0.025	1.37IleIle: 1.37 ± 0.05	0.507IleLys: 0.507 ± 0.024	3.183IleLeu: 3.183 ± 0.068	0.583IleMet: 0.583 ± 0.029	0.616IleAsn: 0.616 ± 0.026	2.089IlePro: 2.089 ± 0.051	0.692IleGln: 0.692 ± 0.029	2.197IleArg: 2.197 ± 0.047	2.046IleSer: 2.046 ± 0.049	2.565IleThr: 2.565 ± 0.056	3.635IleVal: 3.635 ± 0.066	0.413IleTrp: 0.413 ± 0.022	0.548IleTyr: 0.548 ± 0.024	0.0IleXaa: 0.0 ± 0.0
Lys	1.996LysAla: 1.996 ± 0.054	0.058LysCys: 0.058 ± 0.008	1.202LysAsp: 1.202 ± 0.036	0.981LysGlu: 0.981 ± 0.038	0.335LysPhe: 0.335 ± 0.019	1.465LysGly: 1.465 ± 0.046	0.386LysHis: 0.386 ± 0.018	0.727LysIle: 0.727 ± 0.026	0.66LysLys: 0.66 ± 0.036	1.443LysLeu: 1.443 ± 0.043	0.285LysMet: 0.285 ± 0.018	0.407LysAsn: 0.407 ± 0.023	0.853LysPro: 0.853 ± 0.037	0.548LysGln: 0.548 ± 0.023	1.065LysArg: 1.065 ± 0.04	0.876LysSer: 0.876 ± 0.037	0.869LysThr: 0.869 ± 0.035	1.531LysVal: 1.531 ± 0.042	0.154LysTrp: 0.154 ± 0.012	0.348LysTyr: 0.348 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	15.948LeuAla: 15.948 ± 0.169	0.552LeuCys: 0.552 ± 0.026	7.089LeuAsp: 7.089 ± 0.092	6.394LeuGlu: 6.394 ± 0.083	2.551LeuPhe: 2.551 ± 0.057	9.958LeuGly: 9.958 ± 0.118	2.256LeuHis: 2.256 ± 0.053	3.303LeuIle: 3.303 ± 0.063	1.303LeuLys: 1.303 ± 0.044	12.315LeuLeu: 12.315 ± 0.182	1.77LeuMet: 1.77 ± 0.044	1.41LeuAsn: 1.41 ± 0.036	6.55LeuPro: 6.55 ± 0.089	2.547LeuGln: 2.547 ± 0.048	8.716LeuArg: 8.716 ± 0.107	5.361LeuSer: 5.361 ± 0.081	6.543LeuThr: 6.543 ± 0.087	10.068LeuVal: 10.068 ± 0.129	1.325LeuTrp: 1.325 ± 0.04	1.554LeuTyr: 1.554 ± 0.04	0.0LeuXaa: 0.0 ± 0.0
Met	2.133MetAla: 2.133 ± 0.049	0.083MetCys: 0.083 ± 0.01	0.985MetAsp: 0.985 ± 0.033	0.696MetGlu: 0.696 ± 0.026	0.523MetPhe: 0.523 ± 0.023	1.394MetGly: 1.394 ± 0.044	0.392MetHis: 0.392 ± 0.021	0.917MetIle: 0.917 ± 0.038	0.343MetLys: 0.343 ± 0.02	1.989MetLeu: 1.989 ± 0.05	0.353MetMet: 0.353 ± 0.019	0.426MetAsn: 0.426 ± 0.02	1.108MetPro: 1.108 ± 0.031	0.465MetGln: 0.465 ± 0.021	1.324MetArg: 1.324 ± 0.038	1.431MetSer: 1.431 ± 0.039	1.719MetThr: 1.719 ± 0.036	1.414MetVal: 1.414 ± 0.044	0.178MetTrp: 0.178 ± 0.011	0.295MetTyr: 0.295 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	1.843AsnAla: 1.843 ± 0.04	0.096AsnCys: 0.096 ± 0.01	0.781AsnAsp: 0.781 ± 0.031	0.663AsnGlu: 0.663 ± 0.026	0.414AsnPhe: 0.414 ± 0.022	1.402AsnGly: 1.402 ± 0.039	0.334AsnHis: 0.334 ± 0.018	0.657AsnIle: 0.657 ± 0.025	0.256AsnLys: 0.256 ± 0.017	1.596AsnLeu: 1.596 ± 0.04	0.26AsnMet: 0.26 ± 0.015	0.302AsnAsn: 0.302 ± 0.018	1.208AsnPro: 1.208 ± 0.039	0.458AsnGln: 0.458 ± 0.02	0.975AsnArg: 0.975 ± 0.035	0.76AsnSer: 0.76 ± 0.032	0.878AsnThr: 0.878 ± 0.032	1.266AsnVal: 1.266 ± 0.043	0.223AsnTrp: 0.223 ± 0.016	0.374AsnTyr: 0.374 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	8.822ProAla: 8.822 ± 0.156	0.217ProCys: 0.217 ± 0.015	3.708ProAsp: 3.708 ± 0.062	4.626ProGlu: 4.626 ± 0.067	1.632ProPhe: 1.632 ± 0.041	5.937ProGly: 5.937 ± 0.076	1.209ProHis: 1.209 ± 0.038	1.633ProIle: 1.633 ± 0.046	0.709ProLys: 0.709 ± 0.03	5.615ProLeu: 5.615 ± 0.082	0.946ProMet: 0.946 ± 0.031	0.679ProAsn: 0.679 ± 0.029	2.459ProPro: 2.459 ± 0.063	1.649ProGln: 1.649 ± 0.042	4.142ProArg: 4.142 ± 0.073	3.623ProSer: 3.623 ± 0.076	3.395ProThr: 3.395 ± 0.058	5.177ProVal: 5.177 ± 0.078	0.897ProTrp: 0.897 ± 0.034	1.029ProTyr: 1.029 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	3.329GlnAla: 3.329 ± 0.067	0.122GlnCys: 0.122 ± 0.011	1.692GlnAsp: 1.692 ± 0.043	1.845GlnGlu: 1.845 ± 0.05	0.706GlnPhe: 0.706 ± 0.026	2.44GlnGly: 2.44 ± 0.058	0.614GlnHis: 0.614 ± 0.022	1.268GlnIle: 1.268 ± 0.036	0.634GlnLys: 0.634 ± 0.026	3.02GlnLeu: 3.02 ± 0.059	0.515GlnMet: 0.515 ± 0.023	0.488GlnAsn: 0.488 ± 0.025	1.193GlnPro: 1.193 ± 0.038	1.177GlnGln: 1.177 ± 0.041	2.284GlnArg: 2.284 ± 0.052	1.262GlnSer: 1.262 ± 0.032	1.167GlnThr: 1.167 ± 0.035	2.614GlnVal: 2.614 ± 0.053	0.442GlnTrp: 0.442 ± 0.02	0.558GlnTyr: 0.558 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	9.625ArgAla: 9.625 ± 0.123	0.377ArgCys: 0.377 ± 0.021	4.2ArgAsp: 4.2 ± 0.077	5.36ArgGlu: 5.36 ± 0.085	2.205ArgPhe: 2.205 ± 0.04	6.321ArgGly: 6.321 ± 0.082	1.773ArgHis: 1.773 ± 0.043	3.588ArgIle: 3.588 ± 0.061	1.123ArgLys: 1.123 ± 0.038	7.587ArgLeu: 7.587 ± 0.081	1.789ArgMet: 1.789 ± 0.044	0.99ArgAsn: 0.99 ± 0.031	4.488ArgPro: 4.488 ± 0.07	1.756ArgGln: 1.756 ± 0.045	8.08ArgArg: 8.08 ± 0.11	4.694ArgSer: 4.694 ± 0.078	5.263ArgThr: 5.263 ± 0.07	5.291ArgVal: 5.291 ± 0.07	1.238ArgTrp: 1.238 ± 0.035	1.389ArgTyr: 1.389 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	7.212SerAla: 7.212 ± 0.111	0.306SerCys: 0.306 ± 0.02	2.802SerAsp: 2.802 ± 0.065	2.49SerGlu: 2.49 ± 0.057	1.702SerPhe: 1.702 ± 0.046	5.685SerGly: 5.685 ± 0.079	1.006SerHis: 1.006 ± 0.03	2.06SerIle: 2.06 ± 0.048	0.84SerLys: 0.84 ± 0.036	5.482SerLeu: 5.482 ± 0.076	1.127SerMet: 1.127 ± 0.038	0.782SerAsn: 0.782 ± 0.029	3.371SerPro: 3.371 ± 0.068	1.292SerGln: 1.292 ± 0.039	4.208SerArg: 4.208 ± 0.074	3.712SerSer: 3.712 ± 0.098	3.946SerThr: 3.946 ± 0.071	4.239SerVal: 4.239 ± 0.058	0.917SerTrp: 0.917 ± 0.033	1.038SerTyr: 1.038 ± 0.029	0.0SerXaa: 0.0 ± 0.0
Thr	8.58ThrAla: 8.58 ± 0.101	0.334ThrCys: 0.334 ± 0.017	3.575ThrAsp: 3.575 ± 0.063	3.312ThrGlu: 3.312 ± 0.068	1.675ThrPhe: 1.675 ± 0.038	6.251ThrGly: 6.251 ± 0.084	1.111ThrHis: 1.111 ± 0.034	2.354ThrIle: 2.354 ± 0.047	0.911ThrLys: 0.911 ± 0.033	6.144ThrLeu: 6.144 ± 0.094	0.959ThrMet: 0.959 ± 0.032	0.91ThrAsn: 0.91 ± 0.032	3.941ThrPro: 3.941 ± 0.059	1.324ThrGln: 1.324 ± 0.042	3.765ThrArg: 3.765 ± 0.062	3.674ThrSer: 3.674 ± 0.071	4.2ThrThr: 4.2 ± 0.093	5.859ThrVal: 5.859 ± 0.086	1.025ThrTrp: 1.025 ± 0.033	1.208ThrTyr: 1.208 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	10.652ValAla: 10.652 ± 0.099	0.489ValCys: 0.489 ± 0.023	5.533ValAsp: 5.533 ± 0.083	5.154ValGlu: 5.154 ± 0.081	2.249ValPhe: 2.249 ± 0.056	6.945ValGly: 6.945 ± 0.085	1.943ValHis: 1.943 ± 0.052	3.537ValIle: 3.537 ± 0.064	1.323ValLys: 1.323 ± 0.042	11.006ValLeu: 11.006 ± 0.144	1.593ValMet: 1.593 ± 0.049	1.382ValAsn: 1.382 ± 0.039	5.396ValPro: 5.396 ± 0.083	2.285ValGln: 2.285 ± 0.044	6.375ValArg: 6.375 ± 0.081	4.45ValSer: 4.45 ± 0.072	5.651ValThr: 5.651 ± 0.084	8.53ValVal: 8.53 ± 0.112	0.977ValTrp: 0.977 ± 0.03	1.304ValTyr: 1.304 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.581TrpAla: 1.581 ± 0.037	0.095TrpCys: 0.095 ± 0.009	0.848TrpAsp: 0.848 ± 0.03	0.778TrpGlu: 0.778 ± 0.034	0.484TrpPhe: 0.484 ± 0.024	1.094TrpGly: 1.094 ± 0.038	0.335TrpHis: 0.335 ± 0.019	0.684TrpIle: 0.684 ± 0.029	0.289TrpLys: 0.289 ± 0.017	1.389TrpLeu: 1.389 ± 0.043	0.316TrpMet: 0.316 ± 0.018	0.363TrpAsn: 0.363 ± 0.019	0.616TrpPro: 0.616 ± 0.025	0.519TrpGln: 0.519 ± 0.022	1.105TrpArg: 1.105 ± 0.036	0.84TrpSer: 0.84 ± 0.026	1.034TrpThr: 1.034 ± 0.033	1.055TrpVal: 1.055 ± 0.038	0.379TrpTrp: 0.379 ± 0.021	0.289TrpTyr: 0.289 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.176TyrAla: 2.176 ± 0.048	0.117TyrCys: 0.117 ± 0.01	1.16TyrAsp: 1.16 ± 0.035	1.016TyrGlu: 1.016 ± 0.036	0.603TyrPhe: 0.603 ± 0.026	1.776TyrGly: 1.776 ± 0.048	0.311TyrHis: 0.311 ± 0.017	0.5TyrIle: 0.5 ± 0.026	0.279TyrLys: 0.279 ± 0.017	1.939TyrLeu: 1.939 ± 0.044	0.291TyrMet: 0.291 ± 0.021	0.318TyrAsn: 0.318 ± 0.018	0.909TyrPro: 0.909 ± 0.034	0.494TyrGln: 0.494 ± 0.02	1.45TyrArg: 1.45 ± 0.039	0.935TyrSer: 0.935 ± 0.03	1.081TyrThr: 1.081 ± 0.037	1.487TyrVal: 1.487 ± 0.042	0.298TyrTrp: 0.298 ± 0.019	0.434TyrTyr: 0.434 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2953 proteins (1012604 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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