Halobonum sp. NJ-3-1

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Halorubraceae; Halobaculum

Average proteome isoelectric point is 4.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4124 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D5LCI9|A0A7D5LCI9_9EURY F0F1 ATP synthase subunit C OS=Halobonum sp. NJ-3-1 OX=2743089 GN=HUG12_18085 PE=4 SV=1
MM1 pKa = 7.76TDD3 pKa = 3.41RR4 pKa = 11.84DD5 pKa = 4.16AAPDD9 pKa = 3.97LYY11 pKa = 11.06RR12 pKa = 11.84RR13 pKa = 11.84GLLKK17 pKa = 10.63AAGLAAGGAALPGVTSATPGRR38 pKa = 11.84EE39 pKa = 3.76PGAKK43 pKa = 9.13EE44 pKa = 4.4DD45 pKa = 3.84EE46 pKa = 4.55VLVGVSATADD56 pKa = 3.56DD57 pKa = 4.18VEE59 pKa = 4.78GAVARR64 pKa = 11.84HH65 pKa = 5.13VPGDD69 pKa = 3.65AEE71 pKa = 4.41VVHH74 pKa = 7.25SNEE77 pKa = 3.58TLRR80 pKa = 11.84YY81 pKa = 8.15VAVKK85 pKa = 9.83FPSGAADD92 pKa = 3.3VARR95 pKa = 11.84EE96 pKa = 3.98NFVDD100 pKa = 5.6AITKK104 pKa = 9.94KK105 pKa = 9.86EE106 pKa = 3.95HH107 pKa = 5.5VKK109 pKa = 10.5YY110 pKa = 10.71AEE112 pKa = 4.16PNATHH117 pKa = 7.27RR118 pKa = 11.84AFATPNDD125 pKa = 4.0PQFGDD130 pKa = 3.35QYY132 pKa = 11.78APQQVDD138 pKa = 5.32APDD141 pKa = 3.56AWDD144 pKa = 3.45VTYY147 pKa = 10.76GSSSVTVAVVDD158 pKa = 3.86TGAQYY163 pKa = 10.83DD164 pKa = 4.19HH165 pKa = 7.95PDD167 pKa = 3.45LADD170 pKa = 3.94NFADD174 pKa = 4.32DD175 pKa = 4.52PGYY178 pKa = 11.21DD179 pKa = 3.72FADD182 pKa = 4.97DD183 pKa = 5.58DD184 pKa = 4.75SDD186 pKa = 4.54PYY188 pKa = 11.05PDD190 pKa = 4.36APSDD194 pKa = 3.92EE195 pKa = 4.34YY196 pKa = 11.25HH197 pKa = 5.8GTHH200 pKa = 5.73VAGIAGAVTDD210 pKa = 4.34NGTGVAGVSDD220 pKa = 4.04SEE222 pKa = 4.81LINGRR227 pKa = 11.84ALDD230 pKa = 3.91EE231 pKa = 4.75SGSGSTADD239 pKa = 3.12IADD242 pKa = 4.7AIEE245 pKa = 3.73WAADD249 pKa = 3.67RR250 pKa = 11.84DD251 pKa = 3.91ADD253 pKa = 4.55VLNLSLGGGGYY264 pKa = 9.07TSTMKK269 pKa = 10.7NAVSYY274 pKa = 10.53AYY276 pKa = 10.55DD277 pKa = 3.28NGVFIAAAAGNDD289 pKa = 3.67GTNSVSYY296 pKa = 9.12PAAYY300 pKa = 9.87SEE302 pKa = 4.54CLAVSAVDD310 pKa = 3.85SNGDD314 pKa = 3.34LAYY317 pKa = 10.64FSTTGEE323 pKa = 4.32KK324 pKa = 10.55VEE326 pKa = 4.17LAAPGVDD333 pKa = 3.32VLSTTTEE340 pKa = 3.6ARR342 pKa = 11.84GSYY345 pKa = 7.52EE346 pKa = 4.12TLSGTSMATPVVAGSAGLALAQWDD370 pKa = 3.8VDD372 pKa = 3.58NATLRR377 pKa = 11.84SHH379 pKa = 7.11LKK381 pKa = 9.21DD382 pKa = 3.04TATDD386 pKa = 3.82LGLSSDD392 pKa = 4.03EE393 pKa = 4.18QGSGQVDD400 pKa = 3.57AGAAVATEE408 pKa = 4.41PGDD411 pKa = 3.72GGGGGGGGDD420 pKa = 3.51TTTGSVTDD428 pKa = 4.03SLSGYY433 pKa = 8.61WDD435 pKa = 3.41SDD437 pKa = 3.4CWSWAWEE444 pKa = 3.91YY445 pKa = 11.38GSPSKK450 pKa = 11.09VVIEE454 pKa = 4.39LDD456 pKa = 3.8GPSDD460 pKa = 4.4ADD462 pKa = 3.16FDD464 pKa = 4.96LYY466 pKa = 11.51ANEE469 pKa = 4.99GDD471 pKa = 4.19GTCPTTSSYY480 pKa = 11.37DD481 pKa = 3.52YY482 pKa = 10.58RR483 pKa = 11.84SWSTDD488 pKa = 2.93SQEE491 pKa = 4.57TITVDD496 pKa = 5.02DD497 pKa = 5.53PDD499 pKa = 3.88TSTDD503 pKa = 3.11LHH505 pKa = 7.46VLVDD509 pKa = 4.14SYY511 pKa = 11.59SGSGEE516 pKa = 3.89YY517 pKa = 10.14TLTITEE523 pKa = 4.43HH524 pKa = 5.87EE525 pKa = 4.27

Molecular weight: 53.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D5LDS0|A0A7D5LDS0_9EURY UDP-ManNAc 6-dehydrogenase OS=Halobonum sp. NJ-3-1 OX=2743089 GN=HUG12_20275 PE=4 SV=1
MM1 pKa = 7.7ASFDD5 pKa = 3.5AAEE8 pKa = 3.99RR9 pKa = 11.84RR10 pKa = 11.84TLNKK14 pKa = 9.03MICMRR19 pKa = 11.84CNARR23 pKa = 11.84NSEE26 pKa = 3.99RR27 pKa = 11.84ADD29 pKa = 3.53RR30 pKa = 11.84CRR32 pKa = 11.84KK33 pKa = 9.5CGYY36 pKa = 9.92SRR38 pKa = 11.84LRR40 pKa = 11.84PKK42 pKa = 10.67AKK44 pKa = 10.13EE45 pKa = 3.68PRR47 pKa = 11.84TAA49 pKa = 3.84

Molecular weight: 5.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4124

0

4124

1167256

30

1656

283.0

30.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.474 ± 0.06

0.703 ± 0.015

8.268 ± 0.046

8.524 ± 0.057

3.309 ± 0.027

9.534 ± 0.035

2.002 ± 0.021

3.167 ± 0.036

1.56 ± 0.02

8.993 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.684 ± 0.018

2.177 ± 0.021

4.959 ± 0.031

1.94 ± 0.025

7.152 ± 0.043

5.171 ± 0.031

6.044 ± 0.034

9.626 ± 0.046

1.125 ± 0.015

2.587 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski