Amino acid dipepetide frequency for Halobonum sp. NJ-3-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.027AlaAla: 15.027 ± 0.181
0.79AlaCys: 0.79 ± 0.027
10.631AlaAsp: 10.631 ± 0.108
9.278AlaGlu: 9.278 ± 0.108
4.177AlaPhe: 4.177 ± 0.062
11.452AlaGly: 11.452 ± 0.115
1.816AlaHis: 1.816 ± 0.043
3.869AlaIle: 3.869 ± 0.067
1.618AlaLys: 1.618 ± 0.039
10.883AlaLeu: 10.883 ± 0.124
2.137AlaMet: 2.137 ± 0.045
2.317AlaAsn: 2.317 ± 0.044
4.268AlaPro: 4.268 ± 0.072
1.665AlaGln: 1.665 ± 0.041
6.766AlaArg: 6.766 ± 0.093
5.457AlaSer: 5.457 ± 0.072
6.902AlaThr: 6.902 ± 0.086
11.645AlaVal: 11.645 ± 0.117
1.141AlaTrp: 1.141 ± 0.035
2.9AlaTyr: 2.9 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
0.607CysAla: 0.607 ± 0.021
0.074CysCys: 0.074 ± 0.007
0.547CysAsp: 0.547 ± 0.02
0.6CysGlu: 0.6 ± 0.025
0.182CysPhe: 0.182 ± 0.014
0.865CysGly: 0.865 ± 0.03
0.191CysHis: 0.191 ± 0.012
0.258CysIle: 0.258 ± 0.015
0.084CysLys: 0.084 ± 0.01
0.552CysLeu: 0.552 ± 0.021
0.096CysMet: 0.096 ± 0.01
0.174CysAsn: 0.174 ± 0.011
0.551CysPro: 0.551 ± 0.024
0.136CysGln: 0.136 ± 0.01
0.503CysArg: 0.503 ± 0.022
0.421CysSer: 0.421 ± 0.022
0.387CysThr: 0.387 ± 0.019
0.531CysVal: 0.531 ± 0.021
0.082CysTrp: 0.082 ± 0.008
0.187CysTyr: 0.187 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
11.046AspAla: 11.046 ± 0.119
0.612AspCys: 0.612 ± 0.028
6.953AspAsp: 6.953 ± 0.11
7.484AspGlu: 7.484 ± 0.093
1.85AspPhe: 1.85 ± 0.04
9.486AspGly: 9.486 ± 0.118
1.737AspHis: 1.737 ± 0.041
1.849AspIle: 1.849 ± 0.043
0.723AspLys: 0.723 ± 0.027
7.387AspLeu: 7.387 ± 0.104
1.081AspMet: 1.081 ± 0.032
1.017AspAsn: 1.017 ± 0.036
5.15AspPro: 5.15 ± 0.088
1.276AspGln: 1.276 ± 0.042
6.849AspArg: 6.849 ± 0.092
3.214AspSer: 3.214 ± 0.062
3.332AspThr: 3.332 ± 0.06
8.948AspVal: 8.948 ± 0.106
1.027AspTrp: 1.027 ± 0.032
1.661AspTyr: 1.661 ± 0.036
0.0AspXaa: 0.0 ± 0.0
Glu
9.336GluAla: 9.336 ± 0.106
0.52GluCys: 0.52 ± 0.022
5.621GluAsp: 5.621 ± 0.084
7.303GluGlu: 7.303 ± 0.11
3.255GluPhe: 3.255 ± 0.062
6.913GluGly: 6.913 ± 0.075
2.146GluHis: 2.146 ± 0.043
2.905GluIle: 2.905 ± 0.064
1.473GluLys: 1.473 ± 0.048
7.694GluLeu: 7.694 ± 0.093
1.665GluMet: 1.665 ± 0.041
2.007GluAsn: 2.007 ± 0.041
4.127GluPro: 4.127 ± 0.069
1.961GluGln: 1.961 ± 0.053
8.314GluArg: 8.314 ± 0.114
4.699GluSer: 4.699 ± 0.073
5.39GluThr: 5.39 ± 0.078
6.179GluVal: 6.179 ± 0.066
1.158GluTrp: 1.158 ± 0.033
2.577GluTyr: 2.577 ± 0.055
0.0GluXaa: 0.0 ± 0.0
Phe
3.784PheAla: 3.784 ± 0.066
0.258PheCys: 0.258 ± 0.016
3.206PheAsp: 3.206 ± 0.06
3.124PheGlu: 3.124 ± 0.047
0.978PhePhe: 0.978 ± 0.036
3.509PheGly: 3.509 ± 0.067
0.7PheHis: 0.7 ± 0.025
0.813PheIle: 0.813 ± 0.032
0.382PheLys: 0.382 ± 0.017
3.029PheLeu: 3.029 ± 0.062
0.445PheMet: 0.445 ± 0.017
0.645PheAsn: 0.645 ± 0.023
1.474PhePro: 1.474 ± 0.039
0.675PheGln: 0.675 ± 0.025
2.049PheArg: 2.049 ± 0.046
1.646PheSer: 1.646 ± 0.041
1.829PheThr: 1.829 ± 0.048
3.347PheVal: 3.347 ± 0.063
0.392PheTrp: 0.392 ± 0.021
0.81PheTyr: 0.81 ± 0.025
0.0PheXaa: 0.0 ± 0.0
Gly
9.694GlyAla: 9.694 ± 0.111
0.755GlyCys: 0.755 ± 0.027
7.697GlyAsp: 7.697 ± 0.104
8.254GlyGlu: 8.254 ± 0.093
3.384GlyPhe: 3.384 ± 0.062
9.172GlyGly: 9.172 ± 0.125
1.797GlyHis: 1.797 ± 0.038
3.728GlyIle: 3.728 ± 0.071
1.785GlyLys: 1.785 ± 0.04
7.953GlyLeu: 7.953 ± 0.094
1.927GlyMet: 1.927 ± 0.041
2.23GlyAsn: 2.23 ± 0.055
4.014GlyPro: 4.014 ± 0.075
1.708GlyGln: 1.708 ± 0.041
6.135GlyArg: 6.135 ± 0.085
5.587GlySer: 5.587 ± 0.077
6.701GlyThr: 6.701 ± 0.092
8.834GlyVal: 8.834 ± 0.096
1.21GlyTrp: 1.21 ± 0.03
2.777GlyTyr: 2.777 ± 0.054
0.0GlyXaa: 0.0 ± 0.0
His
2.129HisAla: 2.129 ± 0.042
0.176HisCys: 0.176 ± 0.011
1.803HisAsp: 1.803 ± 0.043
1.772HisGlu: 1.772 ± 0.043
0.562HisPhe: 0.562 ± 0.024
2.195HisGly: 2.195 ± 0.05
0.535HisHis: 0.535 ± 0.025
0.474HisIle: 0.474 ± 0.02
0.241HisLys: 0.241 ± 0.015
1.777HisLeu: 1.777 ± 0.046
0.266HisMet: 0.266 ± 0.018
0.389HisAsn: 0.389 ± 0.02
1.327HisPro: 1.327 ± 0.033
0.383HisGln: 0.383 ± 0.017
1.357HisArg: 1.357 ± 0.033
0.77HisSer: 0.77 ± 0.027
0.968HisThr: 0.968 ± 0.03
2.126HisVal: 2.126 ± 0.037
0.221HisTrp: 0.221 ± 0.015
0.547HisTyr: 0.547 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
3.716IleAla: 3.716 ± 0.062
0.206IleCys: 0.206 ± 0.014
2.82IleAsp: 2.82 ± 0.06
3.244IleGlu: 3.244 ± 0.062
0.686IlePhe: 0.686 ± 0.033
3.217IleGly: 3.217 ± 0.064
0.69IleHis: 0.69 ± 0.023
1.01IleIle: 1.01 ± 0.037
0.511IleLys: 0.511 ± 0.025
2.296IleLeu: 2.296 ± 0.055
0.407IleMet: 0.407 ± 0.022
0.747IleAsn: 0.747 ± 0.031
1.644IlePro: 1.644 ± 0.037
0.802IleGln: 0.802 ± 0.026
2.278IleArg: 2.278 ± 0.044
1.65IleSer: 1.65 ± 0.039
1.854IleThr: 1.854 ± 0.049
2.988IleVal: 2.988 ± 0.055
0.229IleTrp: 0.229 ± 0.016
0.679IleTyr: 0.679 ± 0.024
0.0IleXaa: 0.0 ± 0.0
Lys
1.515LysAla: 1.515 ± 0.04
0.111LysCys: 0.111 ± 0.01
0.882LysAsp: 0.882 ± 0.028
1.158LysGlu: 1.158 ± 0.033
0.49LysPhe: 0.49 ± 0.023
1.253LysGly: 1.253 ± 0.032
0.376LysHis: 0.376 ± 0.017
0.527LysIle: 0.527 ± 0.023
0.376LysLys: 0.376 ± 0.023
1.52LysLeu: 1.52 ± 0.041
0.299LysMet: 0.299 ± 0.019
0.451LysAsn: 0.451 ± 0.022
0.856LysPro: 0.856 ± 0.028
0.515LysGln: 0.515 ± 0.026
1.504LysArg: 1.504 ± 0.037
0.894LysSer: 0.894 ± 0.026
1.015LysThr: 1.015 ± 0.032
1.145LysVal: 1.145 ± 0.033
0.198LysTrp: 0.198 ± 0.014
0.518LysTyr: 0.518 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
11.931LeuAla: 11.931 ± 0.131
0.572LeuCys: 0.572 ± 0.023
7.359LeuAsp: 7.359 ± 0.097
6.77LeuGlu: 6.77 ± 0.086
3.074LeuPhe: 3.074 ± 0.065
8.1LeuGly: 8.1 ± 0.09
1.634LeuHis: 1.634 ± 0.04
2.329LeuIle: 2.329 ± 0.052
1.399LeuLys: 1.399 ± 0.034
8.913LeuLeu: 8.913 ± 0.122
1.223LeuMet: 1.223 ± 0.032
1.756LeuAsn: 1.756 ± 0.042
4.549LeuPro: 4.549 ± 0.058
1.677LeuGln: 1.677 ± 0.042
6.177LeuArg: 6.177 ± 0.077
5.255LeuSer: 5.255 ± 0.063
5.033LeuThr: 5.033 ± 0.071
9.235LeuVal: 9.235 ± 0.116
0.865LeuTrp: 0.865 ± 0.029
2.08LeuTyr: 2.08 ± 0.048
0.0LeuXaa: 0.0 ± 0.0
Met
1.829MetAla: 1.829 ± 0.042
0.108MetCys: 0.108 ± 0.01
1.294MetAsp: 1.294 ± 0.033
1.078MetGlu: 1.078 ± 0.035
0.483MetPhe: 0.483 ± 0.02
1.43MetGly: 1.43 ± 0.038
0.296MetHis: 0.296 ± 0.015
0.53MetIle: 0.53 ± 0.021
0.406MetLys: 0.406 ± 0.018
1.491MetLeu: 1.491 ± 0.038
0.289MetMet: 0.289 ± 0.017
0.58MetAsn: 0.58 ± 0.025
0.803MetPro: 0.803 ± 0.027
0.407MetGln: 0.407 ± 0.019
1.157MetArg: 1.157 ± 0.033
1.393MetSer: 1.393 ± 0.035
1.502MetThr: 1.502 ± 0.034
1.211MetVal: 1.211 ± 0.033
0.154MetTrp: 0.154 ± 0.011
0.403MetTyr: 0.403 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
2.511AsnAla: 2.511 ± 0.045
0.224AsnCys: 0.224 ± 0.014
1.613AsnAsp: 1.613 ± 0.039
1.598AsnGlu: 1.598 ± 0.042
0.634AsnPhe: 0.634 ± 0.023
2.172AsnGly: 2.172 ± 0.049
0.445AsnHis: 0.445 ± 0.02
0.616AsnIle: 0.616 ± 0.026
0.328AsnLys: 0.328 ± 0.019
1.783AsnLeu: 1.783 ± 0.044
0.368AsnMet: 0.368 ± 0.018
0.499AsnAsn: 0.499 ± 0.025
1.508AsnPro: 1.508 ± 0.038
0.506AsnGln: 0.506 ± 0.02
1.635AsnArg: 1.635 ± 0.039
0.919AsnSer: 0.919 ± 0.033
1.114AsnThr: 1.114 ± 0.034
2.39AsnVal: 2.39 ± 0.046
0.318AsnTrp: 0.318 ± 0.018
0.586AsnTyr: 0.586 ± 0.023
0.0AsnXaa: 0.0 ± 0.0
Pro
5.282ProAla: 5.282 ± 0.072
0.267ProCys: 0.267 ± 0.016
5.27ProAsp: 5.27 ± 0.076
4.748ProGlu: 4.748 ± 0.069
1.778ProPhe: 1.778 ± 0.04
4.709ProGly: 4.709 ± 0.067
0.987ProHis: 0.987 ± 0.027
1.61ProIle: 1.61 ± 0.04
0.754ProLys: 0.754 ± 0.03
3.943ProLeu: 3.943 ± 0.058
0.84ProMet: 0.84 ± 0.026
1.145ProAsn: 1.145 ± 0.031
2.421ProPro: 2.421 ± 0.051
0.809ProGln: 0.809 ± 0.025
2.743ProArg: 2.743 ± 0.05
2.627ProSer: 2.627 ± 0.052
3.3ProThr: 3.3 ± 0.063
4.619ProVal: 4.619 ± 0.069
0.544ProTrp: 0.544 ± 0.022
1.191ProTyr: 1.191 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
1.865GlnAla: 1.865 ± 0.039
0.135GlnCys: 0.135 ± 0.01
1.086GlnAsp: 1.086 ± 0.032
1.374GlnGlu: 1.374 ± 0.033
0.904GlnPhe: 0.904 ± 0.025
1.522GlnGly: 1.522 ± 0.036
0.485GlnHis: 0.485 ± 0.023
0.751GlnIle: 0.751 ± 0.028
0.404GlnLys: 0.404 ± 0.021
1.815GlnLeu: 1.815 ± 0.045
0.393GlnMet: 0.393 ± 0.018
0.527GlnAsn: 0.527 ± 0.022
0.893GlnPro: 0.893 ± 0.028
0.756GlnGln: 0.756 ± 0.041
1.602GlnArg: 1.602 ± 0.039
1.159GlnSer: 1.159 ± 0.032
1.133GlnThr: 1.133 ± 0.036
1.608GlnVal: 1.608 ± 0.04
0.308GlnTrp: 0.308 ± 0.02
0.679GlnTyr: 0.679 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
7.233ArgAla: 7.233 ± 0.098
0.514ArgCys: 0.514 ± 0.023
5.317ArgAsp: 5.317 ± 0.082
7.466ArgGlu: 7.466 ± 0.104
2.49ArgPhe: 2.49 ± 0.048
5.651ArgGly: 5.651 ± 0.072
1.344ArgHis: 1.344 ± 0.035
2.751ArgIle: 2.751 ± 0.054
1.318ArgLys: 1.318 ± 0.035
6.359ArgLeu: 6.359 ± 0.083
1.528ArgMet: 1.528 ± 0.036
1.785ArgAsn: 1.785 ± 0.043
3.121ArgPro: 3.121 ± 0.055
1.501ArgGln: 1.501 ± 0.035
5.957ArgArg: 5.957 ± 0.097
3.712ArgSer: 3.712 ± 0.067
4.43ArgThr: 4.43 ± 0.062
6.251ArgVal: 6.251 ± 0.094
0.828ArgTrp: 0.828 ± 0.028
1.967ArgTyr: 1.967 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
5.293SerAla: 5.293 ± 0.071
0.292SerCys: 0.292 ± 0.017
4.022SerAsp: 4.022 ± 0.068
3.981SerGlu: 3.981 ± 0.07
1.859SerPhe: 1.859 ± 0.037
5.435SerGly: 5.435 ± 0.071
0.972SerHis: 0.972 ± 0.028
1.989SerIle: 1.989 ± 0.037
0.96SerLys: 0.96 ± 0.027
4.572SerLeu: 4.572 ± 0.068
1.0SerMet: 1.0 ± 0.03
1.217SerAsn: 1.217 ± 0.034
2.734SerPro: 2.734 ± 0.052
1.055SerGln: 1.055 ± 0.029
3.324SerArg: 3.324 ± 0.063
2.734SerSer: 2.734 ± 0.05
3.365SerThr: 3.365 ± 0.061
4.989SerVal: 4.989 ± 0.07
0.585SerTrp: 0.585 ± 0.022
1.335SerTyr: 1.335 ± 0.029
0.0SerXaa: 0.0 ± 0.0
Thr
6.758ThrAla: 6.758 ± 0.085
0.379ThrCys: 0.379 ± 0.022
5.313ThrAsp: 5.313 ± 0.081
4.291ThrGlu: 4.291 ± 0.064
2.188ThrPhe: 2.188 ± 0.047
5.916ThrGly: 5.916 ± 0.077
1.085ThrHis: 1.085 ± 0.028
2.102ThrIle: 2.102 ± 0.044
0.868ThrLys: 0.868 ± 0.027
5.59ThrLeu: 5.59 ± 0.07
0.982ThrMet: 0.982 ± 0.031
1.353ThrAsn: 1.353 ± 0.033
3.454ThrPro: 3.454 ± 0.068
1.004ThrGln: 1.004 ± 0.033
3.526ThrArg: 3.526 ± 0.073
2.713ThrSer: 2.713 ± 0.054
3.782ThrThr: 3.782 ± 0.062
6.839ThrVal: 6.839 ± 0.093
0.618ThrTrp: 0.618 ± 0.023
1.677ThrTyr: 1.677 ± 0.035
0.0ThrXaa: 0.0 ± 0.0
Val
11.449ValAla: 11.449 ± 0.12
0.683ValCys: 0.683 ± 0.024
7.916ValAsp: 7.916 ± 0.083
8.599ValGlu: 8.599 ± 0.095
2.867ValPhe: 2.867 ± 0.061
8.956ValGly: 8.956 ± 0.106
1.919ValHis: 1.919 ± 0.041
2.579ValIle: 2.579 ± 0.057
1.378ValLys: 1.378 ± 0.033
8.76ValLeu: 8.76 ± 0.108
1.3ValMet: 1.3 ± 0.035
2.027ValAsn: 2.027 ± 0.044
4.936ValPro: 4.936 ± 0.061
1.718ValGln: 1.718 ± 0.042
6.672ValArg: 6.672 ± 0.095
5.165ValSer: 5.165 ± 0.069
6.255ValThr: 6.255 ± 0.084
10.081ValVal: 10.081 ± 0.121
0.845ValTrp: 0.845 ± 0.029
2.153ValTyr: 2.153 ± 0.049
0.0ValXaa: 0.0 ± 0.0
Trp
0.995TrpAla: 0.995 ± 0.03
0.094TrpCys: 0.094 ± 0.009
0.783TrpAsp: 0.783 ± 0.025
0.843TrpGlu: 0.843 ± 0.027
0.455TrpPhe: 0.455 ± 0.02
0.913TrpGly: 0.913 ± 0.029
0.255TrpHis: 0.255 ± 0.015
0.407TrpIle: 0.407 ± 0.018
0.252TrpLys: 0.252 ± 0.016
1.164TrpLeu: 1.164 ± 0.035
0.228TrpMet: 0.228 ± 0.014
0.383TrpAsn: 0.383 ± 0.019
0.463TrpPro: 0.463 ± 0.024
0.283TrpGln: 0.283 ± 0.017
0.927TrpArg: 0.927 ± 0.03
0.565TrpSer: 0.565 ± 0.024
0.825TrpThr: 0.825 ± 0.029
0.868TrpVal: 0.868 ± 0.027
0.198TrpTrp: 0.198 ± 0.013
0.351TrpTyr: 0.351 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.741TyrAla: 2.741 ± 0.047
0.258TyrCys: 0.258 ± 0.016
2.547TyrAsp: 2.547 ± 0.045
2.278TyrGlu: 2.278 ± 0.044
0.798TyrPhe: 0.798 ± 0.026
2.477TyrGly: 2.477 ± 0.052
0.607TyrHis: 0.607 ± 0.025
0.577TyrIle: 0.577 ± 0.022
0.344TyrLys: 0.344 ± 0.019
2.451TyrLeu: 2.451 ± 0.046
0.368TyrMet: 0.368 ± 0.018
0.519TyrAsn: 0.519 ± 0.019
1.294TyrPro: 1.294 ± 0.028
0.561TyrGln: 0.561 ± 0.022
2.047TyrArg: 2.047 ± 0.046
1.133TyrSer: 1.133 ± 0.034
1.324TyrThr: 1.324 ± 0.037
2.423TyrVal: 2.423 ± 0.053
0.333TyrTrp: 0.333 ± 0.017
0.787TyrTyr: 0.787 ± 0.028
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4124 proteins (1167256 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski