Vibrio sp. (strain N418)
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4028 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F9RBC3|F9RBC3_VIBSN LysR family transcriptional regulator OS=Vibrio sp. (strain N418) OX=701176 GN=VIBRN418_15588 PE=3 SV=1
MM1 pKa = 7.81 KK2 pKa = 10.19 IGLFYY7 pKa = 11.11 GSTTCYY13 pKa = 10.67 TEE15 pKa = 3.72 MAAEE19 pKa = 4.21 KK20 pKa = 9.37 MRR22 pKa = 11.84 AIMGDD27 pKa = 3.81 DD28 pKa = 3.78 LVDD31 pKa = 3.04 IHH33 pKa = 6.39 NVKK36 pKa = 10.35 EE37 pKa = 4.54 SPLSTMADD45 pKa = 3.45 YY46 pKa = 11.48 DD47 pKa = 4.21 LLILGISTWDD57 pKa = 3.28 FGEE60 pKa = 4.13 IQEE63 pKa = 4.69 DD64 pKa = 3.43 WLEE67 pKa = 3.86 IWDD70 pKa = 4.89 SINSQPLQGKK80 pKa = 6.44 TVALFGLGDD89 pKa = 3.52 QEE91 pKa = 6.57 GYY93 pKa = 10.74 GEE95 pKa = 4.39 WYY97 pKa = 9.98 LDD99 pKa = 4.29 AMGLLHH105 pKa = 7.2 DD106 pKa = 4.11 QVKK109 pKa = 9.66 QAGATIVGYY118 pKa = 9.38 WPNDD122 pKa = 3.11 EE123 pKa = 4.52 QYY125 pKa = 11.19 QFEE128 pKa = 4.12 ASKK131 pKa = 11.24 ALTEE135 pKa = 4.71 DD136 pKa = 2.9 GSMFVGLALDD146 pKa = 4.02 EE147 pKa = 4.89 DD148 pKa = 4.33 SQYY151 pKa = 11.4 EE152 pKa = 4.05 LSDD155 pKa = 3.36 EE156 pKa = 5.53 RR157 pKa = 11.84 IATWVEE163 pKa = 3.91 QVLTEE168 pKa = 4.1 YY169 pKa = 10.54 QASLL173 pKa = 3.41
Molecular weight: 19.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.712
IPC2_protein 3.795
IPC_protein 3.77
Toseland 3.567
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.668
Rodwell 3.605
Grimsley 3.478
Solomon 3.745
Lehninger 3.694
Nozaki 3.872
DTASelect 4.062
Thurlkill 3.617
EMBOSS 3.681
Sillero 3.897
Patrickios 1.036
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.791
Protein with the highest isoelectric point:
>tr|F9RDA5|F9RDA5_VIBSN Uncharacterized protein OS=Vibrio sp. (strain N418) OX=701176 GN=VIBRN418_05196 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.5 RR3 pKa = 11.84 TFQPTVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 7.65 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.44 NGRR28 pKa = 11.84 KK29 pKa = 9.39 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSKK44 pKa = 10.83
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4028
0
4028
1263308
30
4463
313.6
34.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.889 ± 0.042
1.054 ± 0.014
5.471 ± 0.045
6.282 ± 0.04
4.173 ± 0.027
6.764 ± 0.045
2.263 ± 0.022
6.454 ± 0.029
4.983 ± 0.033
10.457 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.741 ± 0.021
4.193 ± 0.026
3.783 ± 0.024
4.908 ± 0.039
4.471 ± 0.033
6.505 ± 0.033
5.264 ± 0.032
7.065 ± 0.037
1.232 ± 0.017
3.048 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here