Vibrio sp. (strain N418)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio; unclassified Vibrio

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4028 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|F9RBC3|F9RBC3_VIBSN LysR family transcriptional regulator OS=Vibrio sp. (strain N418) OX=701176 GN=VIBRN418_15588 PE=3 SV=1
MM1 pKa = 7.81KK2 pKa = 10.19IGLFYY7 pKa = 11.11GSTTCYY13 pKa = 10.67TEE15 pKa = 3.72MAAEE19 pKa = 4.21KK20 pKa = 9.37MRR22 pKa = 11.84AIMGDD27 pKa = 3.81DD28 pKa = 3.78LVDD31 pKa = 3.04IHH33 pKa = 6.39NVKK36 pKa = 10.35EE37 pKa = 4.54SPLSTMADD45 pKa = 3.45YY46 pKa = 11.48DD47 pKa = 4.21LLILGISTWDD57 pKa = 3.28FGEE60 pKa = 4.13IQEE63 pKa = 4.69DD64 pKa = 3.43WLEE67 pKa = 3.86IWDD70 pKa = 4.89SINSQPLQGKK80 pKa = 6.44TVALFGLGDD89 pKa = 3.52QEE91 pKa = 6.57GYY93 pKa = 10.74GEE95 pKa = 4.39WYY97 pKa = 9.98LDD99 pKa = 4.29AMGLLHH105 pKa = 7.2DD106 pKa = 4.11QVKK109 pKa = 9.66QAGATIVGYY118 pKa = 9.38WPNDD122 pKa = 3.11EE123 pKa = 4.52QYY125 pKa = 11.19QFEE128 pKa = 4.12ASKK131 pKa = 11.24ALTEE135 pKa = 4.71DD136 pKa = 2.9GSMFVGLALDD146 pKa = 4.02EE147 pKa = 4.89DD148 pKa = 4.33SQYY151 pKa = 11.4EE152 pKa = 4.05LSDD155 pKa = 3.36EE156 pKa = 5.53RR157 pKa = 11.84IATWVEE163 pKa = 3.91QVLTEE168 pKa = 4.1YY169 pKa = 10.54QASLL173 pKa = 3.41

Molecular weight:
19.49 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|F9RDA5|F9RDA5_VIBSN Uncharacterized protein OS=Vibrio sp. (strain N418) OX=701176 GN=VIBRN418_05196 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.5RR3 pKa = 11.84TFQPTVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 7.65RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.44NGRR28 pKa = 11.84KK29 pKa = 9.39VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LSKK44 pKa = 10.83

Molecular weight:
5.18 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4028

0

4028

1263308

30

4463

313.6

34.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.889 ± 0.042

1.054 ± 0.014

5.471 ± 0.045

6.282 ± 0.04

4.173 ± 0.027

6.764 ± 0.045

2.263 ± 0.022

6.454 ± 0.029

4.983 ± 0.033

10.457 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.741 ± 0.021

4.193 ± 0.026

3.783 ± 0.024

4.908 ± 0.039

4.471 ± 0.033

6.505 ± 0.033

5.264 ± 0.032

7.065 ± 0.037

1.232 ± 0.017

3.048 ± 0.023

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski