Mycobacterium phage HanShotFirst

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5R959|V5R959_9CAUD Uncharacterized protein OS=Mycobacterium phage HanShotFirst OX=1429904 GN=49 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.37KK3 pKa = 10.04LVAAALLALGVVALTSCEE21 pKa = 4.12DD22 pKa = 4.11DD23 pKa = 4.05SDD25 pKa = 4.05GGPNGVIIVDD35 pKa = 3.97GVPYY39 pKa = 9.96FYY41 pKa = 11.41

Molecular weight: 4.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5RBP4|V5RBP4_9CAUD Uncharacterized protein OS=Mycobacterium phage HanShotFirst OX=1429904 GN=7 PE=4 SV=1
MM1 pKa = 7.01ATEE4 pKa = 4.17VDD6 pKa = 3.34HH7 pKa = 7.09VRR9 pKa = 11.84YY10 pKa = 9.53RR11 pKa = 11.84DD12 pKa = 3.64EE13 pKa = 4.41EE14 pKa = 4.56SPLQAACRR22 pKa = 11.84SCHH25 pKa = 4.97GRR27 pKa = 11.84KK28 pKa = 8.93SAQEE32 pKa = 4.02GVAQRR37 pKa = 11.84AKK39 pKa = 10.29LRR41 pKa = 11.84AMKK44 pKa = 9.94KK45 pKa = 9.63RR46 pKa = 11.84PPPRR50 pKa = 11.84HH51 pKa = 5.89PGRR54 pKa = 11.84RR55 pKa = 11.84TPRR58 pKa = 11.84EE59 pKa = 3.85GQEE62 pKa = 3.82PSS64 pKa = 3.0

Molecular weight: 7.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

16527

24

823

181.6

19.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.05 ± 0.456

0.66 ± 0.112

6.716 ± 0.221

6.668 ± 0.255

3.11 ± 0.147

8.562 ± 0.388

1.882 ± 0.174

4.949 ± 0.234

4.078 ± 0.247

8.713 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.051 ± 0.125

3.098 ± 0.143

5.506 ± 0.26

3.261 ± 0.186

6.238 ± 0.396

5.839 ± 0.243

6.462 ± 0.313

7.37 ± 0.204

2.009 ± 0.124

2.777 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski