Amino acid dipepetide frequency for Mycobacterium phage HanShotFirst

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.768AlaAla: 12.768 ± 1.35
0.666AlaCys: 0.666 ± 0.213
7.261AlaAsp: 7.261 ± 0.733
6.172AlaGlu: 6.172 ± 0.713
3.086AlaPhe: 3.086 ± 0.493
7.927AlaGly: 7.927 ± 0.785
1.755AlaHis: 1.755 ± 0.38
4.417AlaIle: 4.417 ± 0.593
4.054AlaLys: 4.054 ± 0.505
9.379AlaLeu: 9.379 ± 0.906
2.178AlaMet: 2.178 ± 0.332
2.905AlaAsn: 2.905 ± 0.398
5.143AlaPro: 5.143 ± 0.764
2.905AlaGln: 2.905 ± 0.37
5.567AlaArg: 5.567 ± 0.519
5.749AlaSer: 5.749 ± 0.714
5.93AlaThr: 5.93 ± 0.687
8.169AlaVal: 8.169 ± 0.775
2.057AlaTrp: 2.057 ± 0.384
2.42AlaTyr: 2.42 ± 0.403
0.0AlaXaa: 0.0 ± 0.0
Cys
0.726CysAla: 0.726 ± 0.206
0.061CysCys: 0.061 ± 0.059
0.484CysAsp: 0.484 ± 0.172
0.605CysGlu: 0.605 ± 0.186
0.182CysPhe: 0.182 ± 0.096
0.666CysGly: 0.666 ± 0.21
0.182CysHis: 0.182 ± 0.111
0.182CysIle: 0.182 ± 0.108
0.182CysLys: 0.182 ± 0.104
0.484CysLeu: 0.484 ± 0.229
0.121CysMet: 0.121 ± 0.09
0.363CysAsn: 0.363 ± 0.159
0.484CysPro: 0.484 ± 0.176
0.121CysGln: 0.121 ± 0.08
0.545CysArg: 0.545 ± 0.21
0.363CysSer: 0.363 ± 0.13
0.242CysThr: 0.242 ± 0.113
0.424CysVal: 0.424 ± 0.198
0.0CysTrp: 0.0 ± 0.0
0.182CysTyr: 0.182 ± 0.115
0.0CysXaa: 0.0 ± 0.0
Asp
6.172AspAla: 6.172 ± 0.548
0.726AspCys: 0.726 ± 0.259
4.72AspAsp: 4.72 ± 0.539
4.054AspGlu: 4.054 ± 0.527
1.997AspPhe: 1.997 ± 0.295
6.898AspGly: 6.898 ± 0.641
1.331AspHis: 1.331 ± 0.276
2.965AspIle: 2.965 ± 0.435
2.723AspLys: 2.723 ± 0.457
6.838AspLeu: 6.838 ± 0.803
1.15AspMet: 1.15 ± 0.193
1.573AspAsn: 1.573 ± 0.299
4.901AspPro: 4.901 ± 0.564
1.997AspGln: 1.997 ± 0.414
3.873AspArg: 3.873 ± 0.4
3.026AspSer: 3.026 ± 0.472
4.296AspThr: 4.296 ± 0.416
4.417AspVal: 4.417 ± 0.581
1.634AspTrp: 1.634 ± 0.296
1.876AspTyr: 1.876 ± 0.35
0.0AspXaa: 0.0 ± 0.0
Glu
6.898GluAla: 6.898 ± 0.729
0.242GluCys: 0.242 ± 0.149
4.901GluAsp: 4.901 ± 0.532
5.325GluGlu: 5.325 ± 0.55
1.997GluPhe: 1.997 ± 0.324
4.296GluGly: 4.296 ± 0.436
1.452GluHis: 1.452 ± 0.339
3.691GluIle: 3.691 ± 0.467
2.602GluLys: 2.602 ± 0.432
6.777GluLeu: 6.777 ± 0.558
1.755GluMet: 1.755 ± 0.301
1.634GluAsn: 1.634 ± 0.364
2.844GluPro: 2.844 ± 0.549
2.602GluGln: 2.602 ± 0.375
3.752GluArg: 3.752 ± 0.458
3.268GluSer: 3.268 ± 0.423
3.51GluThr: 3.51 ± 0.417
5.325GluVal: 5.325 ± 0.595
1.331GluTrp: 1.331 ± 0.367
2.481GluTyr: 2.481 ± 0.509
0.0GluXaa: 0.0 ± 0.0
Phe
2.42PheAla: 2.42 ± 0.3
0.242PheCys: 0.242 ± 0.138
2.965PheAsp: 2.965 ± 0.337
2.057PheGlu: 2.057 ± 0.346
0.545PhePhe: 0.545 ± 0.145
3.207PheGly: 3.207 ± 0.481
0.545PheHis: 0.545 ± 0.224
1.331PheIle: 1.331 ± 0.255
1.271PheLys: 1.271 ± 0.264
2.602PheLeu: 2.602 ± 0.431
0.787PheMet: 0.787 ± 0.216
0.968PheAsn: 0.968 ± 0.253
1.694PhePro: 1.694 ± 0.311
0.726PheGln: 0.726 ± 0.178
1.876PheArg: 1.876 ± 0.387
2.178PheSer: 2.178 ± 0.435
1.876PheThr: 1.876 ± 0.276
2.178PheVal: 2.178 ± 0.348
0.726PheTrp: 0.726 ± 0.226
0.908PheTyr: 0.908 ± 0.226
0.0PheXaa: 0.0 ± 0.0
Gly
7.08GlyAla: 7.08 ± 0.876
0.605GlyCys: 0.605 ± 0.232
5.749GlyAsp: 5.749 ± 0.524
4.962GlyGlu: 4.962 ± 0.498
2.723GlyPhe: 2.723 ± 0.474
8.048GlyGly: 8.048 ± 1.173
1.694GlyHis: 1.694 ± 0.36
4.962GlyIle: 4.962 ± 0.718
3.51GlyLys: 3.51 ± 0.529
8.108GlyLeu: 8.108 ± 0.887
1.755GlyMet: 1.755 ± 0.377
3.328GlyAsn: 3.328 ± 0.379
3.752GlyPro: 3.752 ± 0.595
2.541GlyGln: 2.541 ± 0.303
5.143GlyArg: 5.143 ± 0.531
5.749GlySer: 5.749 ± 0.661
5.264GlyThr: 5.264 ± 0.645
5.809GlyVal: 5.809 ± 0.636
2.42GlyTrp: 2.42 ± 0.419
2.42GlyTyr: 2.42 ± 0.335
0.0GlyXaa: 0.0 ± 0.0
His
1.452HisAla: 1.452 ± 0.283
0.061HisCys: 0.061 ± 0.059
1.029HisAsp: 1.029 ± 0.203
1.392HisGlu: 1.392 ± 0.326
0.666HisPhe: 0.666 ± 0.204
1.694HisGly: 1.694 ± 0.343
0.605HisHis: 0.605 ± 0.215
0.908HisIle: 0.908 ± 0.202
0.908HisLys: 0.908 ± 0.268
1.573HisLeu: 1.573 ± 0.319
0.121HisMet: 0.121 ± 0.089
0.303HisAsn: 0.303 ± 0.131
1.029HisPro: 1.029 ± 0.221
0.908HisGln: 0.908 ± 0.225
1.634HisArg: 1.634 ± 0.353
0.605HisSer: 0.605 ± 0.159
0.908HisThr: 0.908 ± 0.209
1.876HisVal: 1.876 ± 0.354
0.545HisTrp: 0.545 ± 0.16
0.605HisTyr: 0.605 ± 0.218
0.0HisXaa: 0.0 ± 0.0
Ile
6.172IleAla: 6.172 ± 0.755
0.182IleCys: 0.182 ± 0.118
3.389IleAsp: 3.389 ± 0.383
3.57IleGlu: 3.57 ± 0.391
0.968IlePhe: 0.968 ± 0.208
4.175IleGly: 4.175 ± 0.466
0.847IleHis: 0.847 ± 0.206
1.694IleIle: 1.694 ± 0.317
1.694IleLys: 1.694 ± 0.298
3.631IleLeu: 3.631 ± 0.493
0.908IleMet: 0.908 ± 0.257
1.876IleAsn: 1.876 ± 0.332
3.147IlePro: 3.147 ± 0.408
1.634IleGln: 1.634 ± 0.34
3.752IleArg: 3.752 ± 0.482
3.389IleSer: 3.389 ± 0.426
3.147IleThr: 3.147 ± 0.385
3.026IleVal: 3.026 ± 0.593
0.726IleTrp: 0.726 ± 0.181
1.573IleTyr: 1.573 ± 0.245
0.0IleXaa: 0.0 ± 0.0
Lys
3.994LysAla: 3.994 ± 0.493
0.121LysCys: 0.121 ± 0.074
2.662LysAsp: 2.662 ± 0.453
2.057LysGlu: 2.057 ± 0.337
1.513LysPhe: 1.513 ± 0.29
2.36LysGly: 2.36 ± 0.336
0.968LysHis: 0.968 ± 0.278
2.178LysIle: 2.178 ± 0.462
1.815LysLys: 1.815 ± 0.331
3.449LysLeu: 3.449 ± 0.403
0.968LysMet: 0.968 ± 0.221
1.573LysAsn: 1.573 ± 0.291
2.905LysPro: 2.905 ± 0.447
1.694LysGln: 1.694 ± 0.282
2.844LysArg: 2.844 ± 0.482
2.239LysSer: 2.239 ± 0.399
2.36LysThr: 2.36 ± 0.354
3.328LysVal: 3.328 ± 0.475
0.787LysTrp: 0.787 ± 0.218
0.968LysTyr: 0.968 ± 0.291
0.0LysXaa: 0.0 ± 0.0
Leu
9.258LeuAla: 9.258 ± 0.915
0.303LeuCys: 0.303 ± 0.16
6.777LeuAsp: 6.777 ± 0.606
5.93LeuGlu: 5.93 ± 0.635
2.178LeuPhe: 2.178 ± 0.423
7.685LeuGly: 7.685 ± 0.907
1.392LeuHis: 1.392 ± 0.332
4.659LeuIle: 4.659 ± 0.646
4.236LeuLys: 4.236 ± 0.487
5.809LeuLeu: 5.809 ± 0.524
1.755LeuMet: 1.755 ± 0.269
2.541LeuAsn: 2.541 ± 0.39
5.264LeuPro: 5.264 ± 0.525
2.602LeuGln: 2.602 ± 0.428
5.809LeuArg: 5.809 ± 0.596
5.506LeuSer: 5.506 ± 0.535
6.596LeuThr: 6.596 ± 0.544
4.962LeuVal: 4.962 ± 0.632
1.15LeuTrp: 1.15 ± 0.312
2.723LeuTyr: 2.723 ± 0.412
0.0LeuXaa: 0.0 ± 0.0
Met
2.36MetAla: 2.36 ± 0.337
0.061MetCys: 0.061 ± 0.069
0.908MetAsp: 0.908 ± 0.22
1.452MetGlu: 1.452 ± 0.29
0.666MetPhe: 0.666 ± 0.171
1.271MetGly: 1.271 ± 0.279
0.242MetHis: 0.242 ± 0.121
0.424MetIle: 0.424 ± 0.177
1.271MetLys: 1.271 ± 0.255
1.15MetLeu: 1.15 ± 0.305
0.303MetMet: 0.303 ± 0.141
0.968MetAsn: 0.968 ± 0.229
1.271MetPro: 1.271 ± 0.278
0.484MetGln: 0.484 ± 0.165
1.392MetArg: 1.392 ± 0.304
1.936MetSer: 1.936 ± 0.395
2.42MetThr: 2.42 ± 0.33
1.089MetVal: 1.089 ± 0.274
0.363MetTrp: 0.363 ± 0.124
0.484MetTyr: 0.484 ± 0.175
0.0MetXaa: 0.0 ± 0.0
Asn
3.026AsnAla: 3.026 ± 0.483
0.121AsnCys: 0.121 ± 0.117
1.815AsnAsp: 1.815 ± 0.402
2.057AsnGlu: 2.057 ± 0.356
0.968AsnPhe: 0.968 ± 0.242
3.631AsnGly: 3.631 ± 0.486
0.605AsnHis: 0.605 ± 0.193
1.392AsnIle: 1.392 ± 0.3
0.666AsnLys: 0.666 ± 0.213
2.178AsnLeu: 2.178 ± 0.354
0.484AsnMet: 0.484 ± 0.134
0.908AsnAsn: 0.908 ± 0.257
2.36AsnPro: 2.36 ± 0.357
0.968AsnGln: 0.968 ± 0.26
1.452AsnArg: 1.452 ± 0.333
1.573AsnSer: 1.573 ± 0.306
1.997AsnThr: 1.997 ± 0.308
2.905AsnVal: 2.905 ± 0.463
0.545AsnTrp: 0.545 ± 0.14
1.331AsnTyr: 1.331 ± 0.273
0.0AsnXaa: 0.0 ± 0.0
Pro
5.204ProAla: 5.204 ± 0.541
0.303ProCys: 0.303 ± 0.136
4.417ProAsp: 4.417 ± 0.416
4.175ProGlu: 4.175 ± 0.559
1.997ProPhe: 1.997 ± 0.389
5.385ProGly: 5.385 ± 0.588
0.787ProHis: 0.787 ± 0.227
2.541ProIle: 2.541 ± 0.397
1.936ProLys: 1.936 ± 0.295
4.72ProLeu: 4.72 ± 0.574
0.908ProMet: 0.908 ± 0.305
1.634ProAsn: 1.634 ± 0.319
2.965ProPro: 2.965 ± 0.452
1.331ProGln: 1.331 ± 0.318
2.723ProArg: 2.723 ± 0.467
3.873ProSer: 3.873 ± 0.489
4.236ProThr: 4.236 ± 0.462
3.691ProVal: 3.691 ± 0.387
0.726ProTrp: 0.726 ± 0.259
1.513ProTyr: 1.513 ± 0.327
0.0ProXaa: 0.0 ± 0.0
Gln
3.026GlnAla: 3.026 ± 0.466
0.121GlnCys: 0.121 ± 0.088
1.452GlnAsp: 1.452 ± 0.374
1.694GlnGlu: 1.694 ± 0.3
1.089GlnPhe: 1.089 ± 0.272
2.723GlnGly: 2.723 ± 0.328
0.545GlnHis: 0.545 ± 0.157
2.602GlnIle: 2.602 ± 0.424
1.392GlnLys: 1.392 ± 0.259
3.631GlnLeu: 3.631 ± 0.463
1.15GlnMet: 1.15 ± 0.243
0.484GlnAsn: 0.484 ± 0.165
1.936GlnPro: 1.936 ± 0.377
1.815GlnGln: 1.815 ± 0.361
1.997GlnArg: 1.997 ± 0.4
1.573GlnSer: 1.573 ± 0.283
1.634GlnThr: 1.634 ± 0.266
2.541GlnVal: 2.541 ± 0.414
0.605GlnTrp: 0.605 ± 0.148
0.605GlnTyr: 0.605 ± 0.156
0.0GlnXaa: 0.0 ± 0.0
Arg
5.204ArgAla: 5.204 ± 0.575
0.847ArgCys: 0.847 ± 0.242
3.147ArgAsp: 3.147 ± 0.482
4.962ArgGlu: 4.962 ± 0.603
2.239ArgPhe: 2.239 ± 0.463
4.901ArgGly: 4.901 ± 0.571
1.029ArgHis: 1.029 ± 0.243
3.328ArgIle: 3.328 ± 0.456
3.207ArgLys: 3.207 ± 0.538
6.051ArgLeu: 6.051 ± 0.695
1.573ArgMet: 1.573 ± 0.31
2.42ArgAsn: 2.42 ± 0.451
2.36ArgPro: 2.36 ± 0.369
1.755ArgGln: 1.755 ± 0.341
4.599ArgArg: 4.599 ± 0.614
3.873ArgSer: 3.873 ± 0.569
3.268ArgThr: 3.268 ± 0.509
4.78ArgVal: 4.78 ± 0.519
1.452ArgTrp: 1.452 ± 0.301
1.392ArgTyr: 1.392 ± 0.263
0.0ArgXaa: 0.0 ± 0.0
Ser
6.293SerAla: 6.293 ± 0.745
0.545SerCys: 0.545 ± 0.184
2.965SerAsp: 2.965 ± 0.365
3.691SerGlu: 3.691 ± 0.515
1.876SerPhe: 1.876 ± 0.384
6.656SerGly: 6.656 ± 0.624
1.271SerHis: 1.271 ± 0.29
2.965SerIle: 2.965 ± 0.418
2.42SerLys: 2.42 ± 0.407
5.143SerLeu: 5.143 ± 0.597
1.392SerMet: 1.392 ± 0.264
1.997SerAsn: 1.997 ± 0.365
2.844SerPro: 2.844 ± 0.455
1.936SerGln: 1.936 ± 0.249
3.147SerArg: 3.147 ± 0.432
3.268SerSer: 3.268 ± 0.552
3.086SerThr: 3.086 ± 0.435
3.873SerVal: 3.873 ± 0.491
1.634SerTrp: 1.634 ± 0.358
1.331SerTyr: 1.331 ± 0.289
0.0SerXaa: 0.0 ± 0.0
Thr
7.14ThrAla: 7.14 ± 0.924
0.303ThrCys: 0.303 ± 0.151
4.659ThrAsp: 4.659 ± 0.656
4.417ThrGlu: 4.417 ± 0.507
2.42ThrPhe: 2.42 ± 0.363
5.93ThrGly: 5.93 ± 0.573
1.271ThrHis: 1.271 ± 0.299
2.723ThrIle: 2.723 ± 0.502
2.602ThrLys: 2.602 ± 0.369
5.506ThrLeu: 5.506 ± 0.593
0.847ThrMet: 0.847 ± 0.183
1.573ThrAsn: 1.573 ± 0.276
4.054ThrPro: 4.054 ± 0.512
2.057ThrGln: 2.057 ± 0.336
3.268ThrArg: 3.268 ± 0.472
3.086ThrSer: 3.086 ± 0.434
4.236ThrThr: 4.236 ± 0.614
5.627ThrVal: 5.627 ± 0.621
1.15ThrTrp: 1.15 ± 0.238
1.755ThrTyr: 1.755 ± 0.316
0.0ThrXaa: 0.0 ± 0.0
Val
7.322ValAla: 7.322 ± 0.678
0.545ValCys: 0.545 ± 0.214
5.567ValAsp: 5.567 ± 0.567
4.841ValGlu: 4.841 ± 0.54
2.481ValPhe: 2.481 ± 0.397
4.236ValGly: 4.236 ± 0.675
1.392ValHis: 1.392 ± 0.248
3.812ValIle: 3.812 ± 0.43
2.783ValLys: 2.783 ± 0.391
5.446ValLeu: 5.446 ± 0.552
1.271ValMet: 1.271 ± 0.311
2.42ValAsn: 2.42 ± 0.414
4.054ValPro: 4.054 ± 0.447
2.481ValGln: 2.481 ± 0.381
5.385ValArg: 5.385 ± 0.608
4.841ValSer: 4.841 ± 0.484
5.87ValThr: 5.87 ± 0.577
5.567ValVal: 5.567 ± 0.727
1.15ValTrp: 1.15 ± 0.283
2.239ValTyr: 2.239 ± 0.387
0.0ValXaa: 0.0 ± 0.0
Trp
1.755TrpAla: 1.755 ± 0.352
0.363TrpCys: 0.363 ± 0.151
1.271TrpAsp: 1.271 ± 0.266
1.029TrpGlu: 1.029 ± 0.224
0.847TrpPhe: 0.847 ± 0.235
1.634TrpGly: 1.634 ± 0.282
0.424TrpHis: 0.424 ± 0.178
1.029TrpIle: 1.029 ± 0.232
0.363TrpLys: 0.363 ± 0.196
2.239TrpLeu: 2.239 ± 0.34
0.363TrpMet: 0.363 ± 0.15
0.424TrpAsn: 0.424 ± 0.153
0.726TrpPro: 0.726 ± 0.253
1.089TrpGln: 1.089 ± 0.222
1.089TrpArg: 1.089 ± 0.279
0.968TrpSer: 0.968 ± 0.281
1.573TrpThr: 1.573 ± 0.349
2.057TrpVal: 2.057 ± 0.341
0.484TrpTrp: 0.484 ± 0.203
0.363TrpTyr: 0.363 ± 0.17
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.239TyrAla: 2.239 ± 0.408
0.182TyrCys: 0.182 ± 0.156
1.029TyrAsp: 1.029 ± 0.297
2.239TyrGlu: 2.239 ± 0.367
0.666TyrPhe: 0.666 ± 0.196
2.299TyrGly: 2.299 ± 0.367
0.484TyrHis: 0.484 ± 0.174
1.694TyrIle: 1.694 ± 0.329
1.15TyrLys: 1.15 ± 0.217
2.42TyrLeu: 2.42 ± 0.346
0.666TyrMet: 0.666 ± 0.151
1.089TyrAsn: 1.089 ± 0.243
1.331TyrPro: 1.331 ± 0.295
0.968TyrGln: 0.968 ± 0.226
2.541TyrArg: 2.541 ± 0.496
1.331TyrSer: 1.331 ± 0.249
2.178TyrThr: 2.178 ± 0.401
2.057TyrVal: 2.057 ± 0.349
0.605TyrTrp: 0.605 ± 0.212
0.605TyrTyr: 0.605 ± 0.214
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 91 proteins (16527 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski