Marinomonas aquimarina
Average proteome isoelectric point is 5.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3360 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1A8T2B5|A0A1A8T2B5_9GAMM Cytidylate kinase OS=Marinomonas aquimarina OX=295068 GN=cmk PE=3 SV=1
MM1 pKa = 7.31 ITTSYY6 pKa = 10.28 YY7 pKa = 9.92 SSSRR11 pKa = 11.84 YY12 pKa = 8.02 STSNNGYY19 pKa = 10.21 DD20 pKa = 3.51 GVVRR24 pKa = 11.84 IASNGSVGTGALLYY38 pKa = 10.49 DD39 pKa = 3.94 GRR41 pKa = 11.84 AVLTAAHH48 pKa = 6.85 LLDD51 pKa = 4.28 GVSSAEE57 pKa = 3.82 VTFLINGVKK66 pKa = 9.33 TRR68 pKa = 11.84 VSSSDD73 pKa = 3.63 YY74 pKa = 10.61 LLHH77 pKa = 7.09 PSADD81 pKa = 3.26 TTAANNDD88 pKa = 3.99 LAILWLDD95 pKa = 3.43 STPITAEE102 pKa = 3.73 RR103 pKa = 11.84 YY104 pKa = 9.06 QIYY107 pKa = 9.92 RR108 pKa = 11.84 DD109 pKa = 3.46 SDD111 pKa = 3.86 EE112 pKa = 4.43 IGQTFTMAGFGQYY125 pKa = 11.0 GEE127 pKa = 4.64 GASATRR133 pKa = 11.84 YY134 pKa = 9.75 NGSDD138 pKa = 3.04 SVRR141 pKa = 11.84 YY142 pKa = 9.04 KK143 pKa = 10.72 AQNTFDD149 pKa = 3.52 VTGDD153 pKa = 3.49 EE154 pKa = 4.81 FKK156 pKa = 10.89 QGLGSEE162 pKa = 4.58 ISWTPASGTQLLADD176 pKa = 4.9 FDD178 pKa = 4.39 NGSAANDD185 pKa = 3.63 AFGMLLGVNDD195 pKa = 4.97 LGLGADD201 pKa = 4.25 EE202 pKa = 5.12 GLIAPGDD209 pKa = 3.85 SGGSAFIDD217 pKa = 4.09 GVIAGVASYY226 pKa = 8.2 TASVSTWWADD236 pKa = 3.13 TDD238 pKa = 3.46 IDD240 pKa = 4.23 EE241 pKa = 4.62 VTNSSFGEE249 pKa = 3.72 LAAWQRR255 pKa = 11.84 VSHH258 pKa = 4.69 YY259 pKa = 9.7 QRR261 pKa = 11.84 WIDD264 pKa = 3.45 QSVRR268 pKa = 11.84 EE269 pKa = 4.83 HH270 pKa = 6.73 YY271 pKa = 10.64 DD272 pKa = 3.25 SAPEE276 pKa = 4.08 SPAEE280 pKa = 4.0 VQLEE284 pKa = 4.36 VVEE287 pKa = 4.65 GDD289 pKa = 3.41 SGSSYY294 pKa = 11.21 AYY296 pKa = 10.71 FMVSFNGDD304 pKa = 2.48 RR305 pKa = 11.84 GMADD309 pKa = 4.36 DD310 pKa = 5.82 IISVDD315 pKa = 3.4 YY316 pKa = 7.95 QTRR319 pKa = 11.84 DD320 pKa = 3.0 GSATAGEE327 pKa = 5.01 DD328 pKa = 3.58 YY329 pKa = 10.95 LALSGTLNIYY339 pKa = 10.76 DD340 pKa = 4.26 DD341 pKa = 3.83 EE342 pKa = 6.1 SYY344 pKa = 11.82 ALIAVEE350 pKa = 4.03 VLGDD354 pKa = 3.46 QTVEE358 pKa = 4.06 DD359 pKa = 5.0 DD360 pKa = 3.51 EE361 pKa = 5.9 AFYY364 pKa = 11.43 LDD366 pKa = 3.35 ITNPLGGSFGDD377 pKa = 3.86 GVVTLTAMRR386 pKa = 11.84 TILNDD391 pKa = 3.67 DD392 pKa = 4.97 LII394 pKa = 6.26
Molecular weight: 41.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.732
IPC_protein 3.77
Toseland 3.528
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.592
Grimsley 3.439
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.139
Thurlkill 3.592
EMBOSS 3.719
Sillero 3.884
Patrickios 0.858
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.789
Protein with the highest isoelectric point:
>tr|A0A1A8TD91|A0A1A8TD91_9GAMM Catecholate siderophore receptor Fiu OS=Marinomonas aquimarina OX=295068 GN=fiu PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.42 RR12 pKa = 11.84 KK13 pKa = 9.36 RR14 pKa = 11.84 SHH16 pKa = 6.15 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 NGRR28 pKa = 11.84 KK29 pKa = 9.16 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.97 VLSAA44 pKa = 4.11
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3360
0
3360
1092500
29
2315
325.1
36.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.492 ± 0.048
0.981 ± 0.014
5.631 ± 0.041
6.431 ± 0.042
3.988 ± 0.026
6.861 ± 0.041
2.328 ± 0.024
5.823 ± 0.034
4.484 ± 0.034
10.734 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.669 ± 0.022
3.684 ± 0.033
4.048 ± 0.031
4.906 ± 0.038
4.927 ± 0.035
6.53 ± 0.038
5.169 ± 0.044
7.139 ± 0.031
1.286 ± 0.019
2.889 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here