Torque teno canis virus
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G3C8H8|G3C8H8_9VIRU Capsid protein OS=Torque teno canis virus OX=687385 PE=3 SV=1
MM1 pKa = 7.57 SDD3 pKa = 2.63 EE4 pKa = 4.75 GYY6 pKa = 10.41 RR7 pKa = 11.84 EE8 pKa = 4.04 LVEE11 pKa = 4.77 SKK13 pKa = 10.44 SAPTTPGPWSPDD25 pKa = 2.9 RR26 pKa = 11.84 EE27 pKa = 3.57 RR28 pKa = 11.84 WKK30 pKa = 10.28 RR31 pKa = 11.84 HH32 pKa = 3.24 EE33 pKa = 4.37 AAWKK37 pKa = 7.37 QHH39 pKa = 5.74 CSWSHH44 pKa = 5.69 GLWCHH49 pKa = 5.39 CHH51 pKa = 7.11 DD52 pKa = 3.66 WTQHH56 pKa = 4.64 LKK58 pKa = 10.31 KK59 pKa = 9.93 ATRR62 pKa = 11.84 EE63 pKa = 4.18 CGSGTEE69 pKa = 4.19 SGEE72 pKa = 4.15 DD73 pKa = 3.35 PAVSFDD79 pKa = 4.32 LVDD82 pKa = 4.22 DD83 pKa = 4.49 AAMLAAAGDD92 pKa = 4.32 AEE94 pKa = 4.55 PGAAGGGG101 pKa = 3.61
Molecular weight: 10.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.086
IPC2_protein 5.118
IPC_protein 5.041
Toseland 5.118
ProMoST 5.245
Dawson 5.105
Bjellqvist 5.207
Wikipedia 5.016
Rodwell 5.041
Grimsley 5.08
Solomon 5.105
Lehninger 5.08
Nozaki 5.27
DTASelect 5.423
Thurlkill 5.169
EMBOSS 5.13
Sillero 5.347
Patrickios 3.897
IPC_peptide 5.118
IPC2_peptide 5.347
IPC2.peptide.svr19 5.287
Protein with the highest isoelectric point:
>tr|G3C8H8|G3C8H8_9VIRU Capsid protein OS=Torque teno canis virus OX=687385 PE=3 SV=1
MM1 pKa = 7.92 RR2 pKa = 11.84 FRR4 pKa = 11.84 YY5 pKa = 9.76 RR6 pKa = 11.84 KK7 pKa = 7.31 WRR9 pKa = 11.84 RR10 pKa = 11.84 PRR12 pKa = 11.84 RR13 pKa = 11.84 LFRR16 pKa = 11.84 SRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 RR21 pKa = 11.84 GYY23 pKa = 10.34 ARR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 WRR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 AWGRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 VRR40 pKa = 11.84 RR41 pKa = 11.84 YY42 pKa = 7.93 RR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 GGTKK50 pKa = 9.07 RR51 pKa = 11.84 QYY53 pKa = 10.34 ISSIMQWNPQHH64 pKa = 7.07 RR65 pKa = 11.84 HH66 pKa = 3.87 LCYY69 pKa = 10.24 IKK71 pKa = 10.86 GITWLCAARR80 pKa = 11.84 ADD82 pKa = 4.68 RR83 pKa = 11.84 ISWPGIRR90 pKa = 11.84 QIAATDD96 pKa = 3.41 STRR99 pKa = 11.84 YY100 pKa = 8.78 WSTILVGGCGVLTLSMDD117 pKa = 3.09 WFFFEE122 pKa = 4.04 NLRR125 pKa = 11.84 WRR127 pKa = 11.84 NRR129 pKa = 11.84 WSHH132 pKa = 6.36 SNVGYY137 pKa = 10.86 DD138 pKa = 3.4 LARR141 pKa = 11.84 YY142 pKa = 7.71 MGTHH146 pKa = 7.33 LYY148 pKa = 9.91 FPPLADD154 pKa = 2.03 IWYY157 pKa = 9.26 IVWTDD162 pKa = 3.43 TEE164 pKa = 5.41 FVDD167 pKa = 4.53 QPKK170 pKa = 10.55 KK171 pKa = 10.28 VMSYY175 pKa = 5.46 THH177 pKa = 6.41 PWILLMTKK185 pKa = 8.25 KK186 pKa = 10.13 HH187 pKa = 5.76 KK188 pKa = 10.36 VIKK191 pKa = 8.33 PRR193 pKa = 11.84 KK194 pKa = 7.5 WNGKK198 pKa = 6.79 GKK200 pKa = 10.51 KK201 pKa = 9.28 IFLKK205 pKa = 10.49 PPAVFNSAWYY215 pKa = 10.09 APGKK219 pKa = 8.97 WCGAGLGRR227 pKa = 11.84 IYY229 pKa = 10.67 FSFINPFKK237 pKa = 10.83 QIMHH241 pKa = 7.09 TGQTEE246 pKa = 3.74 QSFPFAIEE254 pKa = 4.46 LGNTDD259 pKa = 3.5 YY260 pKa = 11.55 DD261 pKa = 3.59 KK262 pKa = 11.33 PPNNKK267 pKa = 9.35 YY268 pKa = 10.41 DD269 pKa = 3.98 FKK271 pKa = 11.06 NTDD274 pKa = 2.45 IVKK277 pKa = 9.95 QVWTAKK283 pKa = 10.1 DD284 pKa = 3.11 KK285 pKa = 10.5 KK286 pKa = 8.87 YY287 pKa = 10.53 HH288 pKa = 5.98 YY289 pKa = 10.0 RR290 pKa = 11.84 ADD292 pKa = 3.25 WGTGDD297 pKa = 4.26 GNAIMLPNTLSPTTPASWLLLEE319 pKa = 4.34 WDD321 pKa = 3.47 APYY324 pKa = 10.31 WLWFWGKK331 pKa = 8.15 TYY333 pKa = 10.92 QDD335 pKa = 4.09 FVSSHH340 pKa = 5.0 TPEE343 pKa = 4.19 GNQYY347 pKa = 10.33 YY348 pKa = 9.59 GQIYY352 pKa = 8.03 VKK354 pKa = 9.12 WWPITNPYY362 pKa = 9.63 DD363 pKa = 3.99 QGPHH367 pKa = 6.68 TYY369 pKa = 10.23 PEE371 pKa = 4.3 DD372 pKa = 4.14 KK373 pKa = 10.21 EE374 pKa = 4.31 WCLVMLDD381 pKa = 3.93 PQPNFMTCNSIAAALKK397 pKa = 8.99 IAQFGPCVYY406 pKa = 10.65 SSTDD410 pKa = 3.45 LQQSQIPINIPMYY423 pKa = 10.61 YY424 pKa = 9.78 LSKK427 pKa = 8.67 WQWGGSTQGTSTHH440 pKa = 6.59 IDD442 pKa = 3.38 NPCNPRR448 pKa = 11.84 PRR450 pKa = 11.84 SIQIADD456 pKa = 3.84 PASAPRR462 pKa = 11.84 DD463 pKa = 3.83 VIHH466 pKa = 6.85 PWDD469 pKa = 3.62 VDD471 pKa = 3.36 ASGTISTAKK480 pKa = 10.24 LKK482 pKa = 10.77 RR483 pKa = 11.84 LMEE486 pKa = 4.28 GLGYY490 pKa = 10.16 RR491 pKa = 11.84 EE492 pKa = 4.9 PKK494 pKa = 9.43 PKK496 pKa = 10.01 PGPPEE501 pKa = 3.73 TLIPPGAPTPEE512 pKa = 3.94 LDD514 pKa = 3.82 YY515 pKa = 11.33 YY516 pKa = 11.39 SSEE519 pKa = 4.28 TEE521 pKa = 4.02 SDD523 pKa = 3.98 DD524 pKa = 4.61 FDD526 pKa = 3.97 TGDD529 pKa = 3.66 SEE531 pKa = 5.41 EE532 pKa = 4.56 EE533 pKa = 4.31 EE534 pKa = 4.96 EE535 pKa = 4.74 DD536 pKa = 3.53 HH537 pKa = 7.4 QDD539 pKa = 3.48 PRR541 pKa = 11.84 WVRR544 pKa = 11.84 EE545 pKa = 4.07 SLDD548 pKa = 3.13 KK549 pKa = 10.3 LTRR552 pKa = 11.84 RR553 pKa = 11.84 LRR555 pKa = 11.84 GEE557 pKa = 3.6 RR558 pKa = 11.84 RR559 pKa = 11.84 QRR561 pKa = 11.84 QQLGKK566 pKa = 10.88 GLLALLKK573 pKa = 10.5 DD574 pKa = 4.0 KK575 pKa = 10.97 KK576 pKa = 10.92
Molecular weight: 67.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.919
IPC2_protein 9.355
IPC_protein 9.428
Toseland 9.853
ProMoST 9.677
Dawson 10.131
Bjellqvist 9.853
Wikipedia 10.321
Rodwell 10.394
Grimsley 10.218
Solomon 10.145
Lehninger 10.101
Nozaki 9.897
DTASelect 9.823
Thurlkill 9.955
EMBOSS 10.292
Sillero 10.043
Patrickios 5.626
IPC_peptide 10.145
IPC2_peptide 8.624
IPC2.peptide.svr19 8.336
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
920
101
576
306.7
35.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.63 ± 1.882
1.848 ± 0.628
6.304 ± 0.502
5.217 ± 1.537
2.609 ± 0.802
7.5 ± 1.005
2.717 ± 0.886
3.587 ± 1.82
5.652 ± 0.68
6.522 ± 0.437
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.065 ± 0.025
2.609 ± 0.925
7.935 ± 0.589
3.804 ± 0.608
9.13 ± 1.121
7.174 ± 1.283
6.848 ± 0.845
3.043 ± 0.072
4.891 ± 0.457
3.913 ± 1.253
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here