Bovine parainfluenza 3 virus (BPIV-3)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Orthoparamyxovirinae; Respirovirus

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0F6N3B3|A0A0F6N3B3_PI3B RNA-directed RNA polymerase L OS=Bovine parainfluenza 3 virus OX=11215 GN=L PE=3 SV=1
MM1 pKa = 7.66LSLFDD6 pKa = 3.55TFSARR11 pKa = 11.84RR12 pKa = 11.84QEE14 pKa = 4.8NITKK18 pKa = 10.01SAGGAVIPGQKK29 pKa = 8.52NTVSIFALGPSITDD43 pKa = 4.2DD44 pKa = 3.65NDD46 pKa = 3.31KK47 pKa = 8.61MTLALLFLSHH57 pKa = 7.05ALDD60 pKa = 3.55NEE62 pKa = 4.22KK63 pKa = 10.1QHH65 pKa = 5.8AQRR68 pKa = 11.84AGFLVSLLSMAYY80 pKa = 10.11ANPEE84 pKa = 4.09LYY86 pKa = 9.32LTANGSNADD95 pKa = 3.69VKK97 pKa = 10.62YY98 pKa = 10.03VIYY101 pKa = 9.65MIEE104 pKa = 4.29KK105 pKa = 9.95DD106 pKa = 3.63PGRR109 pKa = 11.84QKK111 pKa = 11.17YY112 pKa = 10.05GGLIIKK118 pKa = 7.97TRR120 pKa = 11.84EE121 pKa = 3.61MVYY124 pKa = 10.81EE125 pKa = 4.22KK126 pKa = 8.77TTDD129 pKa = 2.75WMFGSDD135 pKa = 5.39LEE137 pKa = 4.17YY138 pKa = 11.13DD139 pKa = 3.85QDD141 pKa = 4.46NMLQNGRR148 pKa = 11.84STSTIEE154 pKa = 4.64DD155 pKa = 3.65LVHH158 pKa = 5.73TFGYY162 pKa = 9.26PSCLGALIIQIWIILVKK179 pKa = 10.56AITSISGLRR188 pKa = 11.84KK189 pKa = 9.95GFFTRR194 pKa = 11.84LEE196 pKa = 4.02AFRR199 pKa = 11.84QDD201 pKa = 3.25GTVKK205 pKa = 10.6SSLVLSGDD213 pKa = 3.29AVEE216 pKa = 4.69QIGSIMRR223 pKa = 11.84SQQSLVTLMVEE234 pKa = 4.35TLITMNTGRR243 pKa = 11.84NDD245 pKa = 2.97LTTIEE250 pKa = 4.61KK251 pKa = 10.41NIQIVGNYY259 pKa = 8.94IRR261 pKa = 11.84DD262 pKa = 3.47AGLASFFNTIRR273 pKa = 11.84YY274 pKa = 9.22GIEE277 pKa = 3.27TRR279 pKa = 11.84MAALTLSTLRR289 pKa = 11.84PDD291 pKa = 3.44INRR294 pKa = 11.84LKK296 pKa = 11.05ALMEE300 pKa = 4.81LYY302 pKa = 10.37LSKK305 pKa = 10.93GPRR308 pKa = 11.84APFICILRR316 pKa = 11.84DD317 pKa = 3.48PVHH320 pKa = 7.27GEE322 pKa = 4.12FAPGNYY328 pKa = 7.59PALWSYY334 pKa = 11.87AMGVAVVQNKK344 pKa = 9.23AMQQYY349 pKa = 7.85VTGRR353 pKa = 11.84SYY355 pKa = 11.79LDD357 pKa = 2.92IEE359 pKa = 4.49MFQLGQAVARR369 pKa = 11.84DD370 pKa = 4.22AEE372 pKa = 4.51SQMSSILEE380 pKa = 4.26DD381 pKa = 3.33EE382 pKa = 4.8LGITQEE388 pKa = 4.54AKK390 pKa = 10.37QSLKK394 pKa = 10.79NHH396 pKa = 6.09MKK398 pKa = 10.42RR399 pKa = 11.84ISSSDD404 pKa = 3.21TAFQKK409 pKa = 9.39PTGGAAIEE417 pKa = 3.94MAIDD421 pKa = 3.76EE422 pKa = 4.62EE423 pKa = 4.96AEE425 pKa = 4.21QPDD428 pKa = 3.61SRR430 pKa = 11.84GDD432 pKa = 3.42QDD434 pKa = 3.78QSNGTQSSIIPYY446 pKa = 10.06AWADD450 pKa = 3.47DD451 pKa = 4.31TNNGTQTEE459 pKa = 4.69SATEE463 pKa = 3.69IDD465 pKa = 5.19SITTEE470 pKa = 3.58QRR472 pKa = 11.84NIRR475 pKa = 11.84DD476 pKa = 3.76RR477 pKa = 11.84LNKK480 pKa = 9.93RR481 pKa = 11.84LNEE484 pKa = 3.76KK485 pKa = 10.29RR486 pKa = 11.84KK487 pKa = 9.52QNEE490 pKa = 3.85LRR492 pKa = 11.84SDD494 pKa = 3.64DD495 pKa = 3.78TTDD498 pKa = 3.06NTNQTEE504 pKa = 3.99IDD506 pKa = 3.87DD507 pKa = 4.37LFSAFGSNN515 pKa = 3.34

Molecular weight:
57.27 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0F6N3J2|A0A0F6N3J2_PI3B Fusion glycoprotein F0 OS=Bovine parainfluenza 3 virus OX=11215 GN=F PE=3 SV=1
MM1 pKa = 7.57SIKK4 pKa = 10.59NSAIYY9 pKa = 8.33TFPDD13 pKa = 3.74SSFLDD18 pKa = 3.57NGNIEE23 pKa = 4.19PLPLKK28 pKa = 10.86VNEE31 pKa = 3.85QRR33 pKa = 11.84KK34 pKa = 8.51AVPHH38 pKa = 5.6IRR40 pKa = 11.84VVKK43 pKa = 10.55IGDD46 pKa = 3.75PPKK49 pKa = 10.55HH50 pKa = 5.98GSRR53 pKa = 11.84YY54 pKa = 10.04LDD56 pKa = 3.28VFLLGFFEE64 pKa = 4.52MEE66 pKa = 3.99RR67 pKa = 11.84SKK69 pKa = 11.36DD70 pKa = 3.45RR71 pKa = 11.84YY72 pKa = 10.76GSISDD77 pKa = 4.9LDD79 pKa = 4.49DD80 pKa = 4.59DD81 pKa = 4.99PSYY84 pKa = 10.83KK85 pKa = 10.39VCGSGSLPLGLAKK98 pKa = 9.91YY99 pKa = 8.79TGGDD103 pKa = 3.49QEE105 pKa = 4.96LLQAAIKK112 pKa = 10.62LDD114 pKa = 3.28IEE116 pKa = 4.2VRR118 pKa = 11.84RR119 pKa = 11.84TVKK122 pKa = 8.8ATEE125 pKa = 4.15MIVYY129 pKa = 7.68TVQNIKK135 pKa = 10.55PEE137 pKa = 4.48LYY139 pKa = 8.64PWSGRR144 pKa = 11.84LRR146 pKa = 11.84KK147 pKa = 10.13GMLFDD152 pKa = 4.35ANKK155 pKa = 10.02VALAPQCLPLDD166 pKa = 3.73RR167 pKa = 11.84GIKK170 pKa = 9.81FRR172 pKa = 11.84VIFVNCTAIGSITLFKK188 pKa = 10.31IPKK191 pKa = 9.93SMALLSLPNTISINLQVHH209 pKa = 6.55IKK211 pKa = 9.71TGIQTDD217 pKa = 3.63SKK219 pKa = 11.29GVVQILDD226 pKa = 3.61EE227 pKa = 4.79KK228 pKa = 11.28GEE230 pKa = 3.98KK231 pKa = 9.96SLNFMVHH238 pKa = 6.48LGLIKK243 pKa = 10.4RR244 pKa = 11.84KK245 pKa = 8.81IGRR248 pKa = 11.84MYY250 pKa = 10.53SVEE253 pKa = 3.94YY254 pKa = 10.17CKK256 pKa = 10.57QKK258 pKa = 9.97IEE260 pKa = 4.15KK261 pKa = 8.94MRR263 pKa = 11.84LLFSLGLVGGISFHH277 pKa = 6.95VNATGSISKK286 pKa = 8.63TLASQLAFKK295 pKa = 10.94RR296 pKa = 11.84EE297 pKa = 3.5ICYY300 pKa = 10.23PLMDD304 pKa = 5.94LNPHH308 pKa = 6.39LNSVIWASSIEE319 pKa = 4.07ITRR322 pKa = 11.84VDD324 pKa = 5.07AILQPSLPGEE334 pKa = 3.7FRR336 pKa = 11.84YY337 pKa = 10.34YY338 pKa = 10.39PNIIAKK344 pKa = 9.88GVGKK348 pKa = 10.41IKK350 pKa = 10.5QQ351 pKa = 3.19

Molecular weight:
39.17 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

4807

351

2233

801.2

90.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.722 ± 0.473

1.435 ± 0.277

6.137 ± 0.276

5.804 ± 0.694

3.016 ± 0.369

5.596 ± 0.47

1.685 ± 0.224

9.341 ± 0.843

6.532 ± 0.449

9.673 ± 0.702

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.413 ± 0.274

6.511 ± 0.402

3.828 ± 0.294

3.828 ± 0.543

4.681 ± 0.38

8.134 ± 0.329

6.782 ± 0.58

4.91 ± 0.388

1.144 ± 0.213

3.828 ± 0.451

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski