Bovine parainfluenza 3 virus (BPIV-3)
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F6N3B3|A0A0F6N3B3_PI3B RNA-directed RNA polymerase L OS=Bovine parainfluenza 3 virus OX=11215 GN=L PE=3 SV=1
MM1 pKa = 7.66 LSLFDD6 pKa = 3.55 TFSARR11 pKa = 11.84 RR12 pKa = 11.84 QEE14 pKa = 4.8 NITKK18 pKa = 10.01 SAGGAVIPGQKK29 pKa = 8.52 NTVSIFALGPSITDD43 pKa = 4.2 DD44 pKa = 3.65 NDD46 pKa = 3.31 KK47 pKa = 8.61 MTLALLFLSHH57 pKa = 7.05 ALDD60 pKa = 3.55 NEE62 pKa = 4.22 KK63 pKa = 10.1 QHH65 pKa = 5.8 AQRR68 pKa = 11.84 AGFLVSLLSMAYY80 pKa = 10.11 ANPEE84 pKa = 4.09 LYY86 pKa = 9.32 LTANGSNADD95 pKa = 3.69 VKK97 pKa = 10.62 YY98 pKa = 10.03 VIYY101 pKa = 9.65 MIEE104 pKa = 4.29 KK105 pKa = 9.95 DD106 pKa = 3.63 PGRR109 pKa = 11.84 QKK111 pKa = 11.17 YY112 pKa = 10.05 GGLIIKK118 pKa = 7.97 TRR120 pKa = 11.84 EE121 pKa = 3.61 MVYY124 pKa = 10.81 EE125 pKa = 4.22 KK126 pKa = 8.77 TTDD129 pKa = 2.75 WMFGSDD135 pKa = 5.39 LEE137 pKa = 4.17 YY138 pKa = 11.13 DD139 pKa = 3.85 QDD141 pKa = 4.46 NMLQNGRR148 pKa = 11.84 STSTIEE154 pKa = 4.64 DD155 pKa = 3.65 LVHH158 pKa = 5.73 TFGYY162 pKa = 9.26 PSCLGALIIQIWIILVKK179 pKa = 10.56 AITSISGLRR188 pKa = 11.84 KK189 pKa = 9.95 GFFTRR194 pKa = 11.84 LEE196 pKa = 4.02 AFRR199 pKa = 11.84 QDD201 pKa = 3.25 GTVKK205 pKa = 10.6 SSLVLSGDD213 pKa = 3.29 AVEE216 pKa = 4.69 QIGSIMRR223 pKa = 11.84 SQQSLVTLMVEE234 pKa = 4.35 TLITMNTGRR243 pKa = 11.84 NDD245 pKa = 2.97 LTTIEE250 pKa = 4.61 KK251 pKa = 10.41 NIQIVGNYY259 pKa = 8.94 IRR261 pKa = 11.84 DD262 pKa = 3.47 AGLASFFNTIRR273 pKa = 11.84 YY274 pKa = 9.22 GIEE277 pKa = 3.27 TRR279 pKa = 11.84 MAALTLSTLRR289 pKa = 11.84 PDD291 pKa = 3.44 INRR294 pKa = 11.84 LKK296 pKa = 11.05 ALMEE300 pKa = 4.81 LYY302 pKa = 10.37 LSKK305 pKa = 10.93 GPRR308 pKa = 11.84 APFICILRR316 pKa = 11.84 DD317 pKa = 3.48 PVHH320 pKa = 7.27 GEE322 pKa = 4.12 FAPGNYY328 pKa = 7.59 PALWSYY334 pKa = 11.87 AMGVAVVQNKK344 pKa = 9.23 AMQQYY349 pKa = 7.85 VTGRR353 pKa = 11.84 SYY355 pKa = 11.79 LDD357 pKa = 2.92 IEE359 pKa = 4.49 MFQLGQAVARR369 pKa = 11.84 DD370 pKa = 4.22 AEE372 pKa = 4.51 SQMSSILEE380 pKa = 4.26 DD381 pKa = 3.33 EE382 pKa = 4.8 LGITQEE388 pKa = 4.54 AKK390 pKa = 10.37 QSLKK394 pKa = 10.79 NHH396 pKa = 6.09 MKK398 pKa = 10.42 RR399 pKa = 11.84 ISSSDD404 pKa = 3.21 TAFQKK409 pKa = 9.39 PTGGAAIEE417 pKa = 3.94 MAIDD421 pKa = 3.76 EE422 pKa = 4.62 EE423 pKa = 4.96 AEE425 pKa = 4.21 QPDD428 pKa = 3.61 SRR430 pKa = 11.84 GDD432 pKa = 3.42 QDD434 pKa = 3.78 QSNGTQSSIIPYY446 pKa = 10.06 AWADD450 pKa = 3.47 DD451 pKa = 4.31 TNNGTQTEE459 pKa = 4.69 SATEE463 pKa = 3.69 IDD465 pKa = 5.19 SITTEE470 pKa = 3.58 QRR472 pKa = 11.84 NIRR475 pKa = 11.84 DD476 pKa = 3.76 RR477 pKa = 11.84 LNKK480 pKa = 9.93 RR481 pKa = 11.84 LNEE484 pKa = 3.76 KK485 pKa = 10.29 RR486 pKa = 11.84 KK487 pKa = 9.52 QNEE490 pKa = 3.85 LRR492 pKa = 11.84 SDD494 pKa = 3.64 DD495 pKa = 3.78 TTDD498 pKa = 3.06 NTNQTEE504 pKa = 3.99 IDD506 pKa = 3.87 DD507 pKa = 4.37 LFSAFGSNN515 pKa = 3.34
Molecular weight: 57.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.978
IPC2_protein 4.978
IPC_protein 4.927
Toseland 4.8
ProMoST 5.041
Dawson 4.889
Bjellqvist 5.029
Wikipedia 4.774
Rodwell 4.787
Grimsley 4.711
Solomon 4.889
Lehninger 4.851
Nozaki 5.003
DTASelect 5.181
Thurlkill 4.8
EMBOSS 4.787
Sillero 5.067
Patrickios 4.228
IPC_peptide 4.902
IPC2_peptide 5.054
IPC2.peptide.svr19 5.086
Protein with the highest isoelectric point:
>tr|A0A0F6N3J2|A0A0F6N3J2_PI3B Fusion glycoprotein F0 OS=Bovine parainfluenza 3 virus OX=11215 GN=F PE=3 SV=1
MM1 pKa = 7.57 SIKK4 pKa = 10.59 NSAIYY9 pKa = 8.33 TFPDD13 pKa = 3.74 SSFLDD18 pKa = 3.57 NGNIEE23 pKa = 4.19 PLPLKK28 pKa = 10.86 VNEE31 pKa = 3.85 QRR33 pKa = 11.84 KK34 pKa = 8.51 AVPHH38 pKa = 5.6 IRR40 pKa = 11.84 VVKK43 pKa = 10.55 IGDD46 pKa = 3.75 PPKK49 pKa = 10.55 HH50 pKa = 5.98 GSRR53 pKa = 11.84 YY54 pKa = 10.04 LDD56 pKa = 3.28 VFLLGFFEE64 pKa = 4.52 MEE66 pKa = 3.99 RR67 pKa = 11.84 SKK69 pKa = 11.36 DD70 pKa = 3.45 RR71 pKa = 11.84 YY72 pKa = 10.76 GSISDD77 pKa = 4.9 LDD79 pKa = 4.49 DD80 pKa = 4.59 DD81 pKa = 4.99 PSYY84 pKa = 10.83 KK85 pKa = 10.39 VCGSGSLPLGLAKK98 pKa = 9.91 YY99 pKa = 8.79 TGGDD103 pKa = 3.49 QEE105 pKa = 4.96 LLQAAIKK112 pKa = 10.62 LDD114 pKa = 3.28 IEE116 pKa = 4.2 VRR118 pKa = 11.84 RR119 pKa = 11.84 TVKK122 pKa = 8.8 ATEE125 pKa = 4.15 MIVYY129 pKa = 7.68 TVQNIKK135 pKa = 10.55 PEE137 pKa = 4.48 LYY139 pKa = 8.64 PWSGRR144 pKa = 11.84 LRR146 pKa = 11.84 KK147 pKa = 10.13 GMLFDD152 pKa = 4.35 ANKK155 pKa = 10.02 VALAPQCLPLDD166 pKa = 3.73 RR167 pKa = 11.84 GIKK170 pKa = 9.81 FRR172 pKa = 11.84 VIFVNCTAIGSITLFKK188 pKa = 10.31 IPKK191 pKa = 9.93 SMALLSLPNTISINLQVHH209 pKa = 6.55 IKK211 pKa = 9.71 TGIQTDD217 pKa = 3.63 SKK219 pKa = 11.29 GVVQILDD226 pKa = 3.61 EE227 pKa = 4.79 KK228 pKa = 11.28 GEE230 pKa = 3.98 KK231 pKa = 9.96 SLNFMVHH238 pKa = 6.48 LGLIKK243 pKa = 10.4 RR244 pKa = 11.84 KK245 pKa = 8.81 IGRR248 pKa = 11.84 MYY250 pKa = 10.53 SVEE253 pKa = 3.94 YY254 pKa = 10.17 CKK256 pKa = 10.57 QKK258 pKa = 9.97 IEE260 pKa = 4.15 KK261 pKa = 8.94 MRR263 pKa = 11.84 LLFSLGLVGGISFHH277 pKa = 6.95 VNATGSISKK286 pKa = 8.63 TLASQLAFKK295 pKa = 10.94 RR296 pKa = 11.84 EE297 pKa = 3.5 ICYY300 pKa = 10.23 PLMDD304 pKa = 5.94 LNPHH308 pKa = 6.39 LNSVIWASSIEE319 pKa = 4.07 ITRR322 pKa = 11.84 VDD324 pKa = 5.07 AILQPSLPGEE334 pKa = 3.7 FRR336 pKa = 11.84 YY337 pKa = 10.34 YY338 pKa = 10.39 PNIIAKK344 pKa = 9.88 GVGKK348 pKa = 10.41 IKK350 pKa = 10.5 QQ351 pKa = 3.19
Molecular weight: 39.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.889
IPC2_protein 8.858
IPC_protein 8.741
Toseland 9.56
ProMoST 9.268
Dawson 9.809
Bjellqvist 9.502
Wikipedia 9.955
Rodwell 10.189
Grimsley 9.867
Solomon 9.823
Lehninger 9.794
Nozaki 9.663
DTASelect 9.458
Thurlkill 9.648
EMBOSS 9.984
Sillero 9.75
Patrickios 5.385
IPC_peptide 9.823
IPC2_peptide 8.068
IPC2.peptide.svr19 7.974
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4807
351
2233
801.2
90.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.722 ± 0.473
1.435 ± 0.277
6.137 ± 0.276
5.804 ± 0.694
3.016 ± 0.369
5.596 ± 0.47
1.685 ± 0.224
9.341 ± 0.843
6.532 ± 0.449
9.673 ± 0.702
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.413 ± 0.274
6.511 ± 0.402
3.828 ± 0.294
3.828 ± 0.543
4.681 ± 0.38
8.134 ± 0.329
6.782 ± 0.58
4.91 ± 0.388
1.144 ± 0.213
3.828 ± 0.451
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here