Hubei tombus-like virus 19

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGU0|A0A1L3KGU0_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 19 OX=1923265 PE=4 SV=1
MM1 pKa = 7.71EE2 pKa = 5.09NLSRR6 pKa = 11.84PSSLSCGKK14 pKa = 8.68FTMEE18 pKa = 4.07KK19 pKa = 10.25SKK21 pKa = 9.49TRR23 pKa = 11.84SPRR26 pKa = 11.84STNLRR31 pKa = 11.84EE32 pKa = 4.21SNEE35 pKa = 4.15KK36 pKa = 10.21IVTNVLLARR45 pKa = 11.84LSEE48 pKa = 3.96QLEE51 pKa = 4.6TINRR55 pKa = 11.84RR56 pKa = 11.84ISLIEE61 pKa = 4.63DD62 pKa = 3.31SLQEE66 pKa = 4.25SNRR69 pKa = 11.84SQLQLILSISRR80 pKa = 11.84LEE82 pKa = 4.06KK83 pKa = 9.93EE84 pKa = 4.62CRR86 pKa = 11.84QGVPGANSPTEE97 pKa = 4.03SRR99 pKa = 11.84AKK101 pKa = 10.29SSKK104 pKa = 10.26KK105 pKa = 10.48LPSCRR110 pKa = 11.84TNPTIPPFRR119 pKa = 11.84SRR121 pKa = 11.84QEE123 pKa = 3.98SFSSIDD129 pKa = 3.5SLEE132 pKa = 4.0SMYY135 pKa = 10.49PGPPQRR141 pKa = 11.84HH142 pKa = 6.22LGRR145 pKa = 11.84PAMNEE150 pKa = 3.51ALIRR154 pKa = 11.84EE155 pKa = 4.71LNTTLHH161 pKa = 6.67ASCGGQTASSSHH173 pKa = 7.12PDD175 pKa = 3.21SEE177 pKa = 4.78GCGIVSPGDD186 pKa = 3.54NNNEE190 pKa = 4.19SVPTKK195 pKa = 10.01PSPLTSQSACISGPQIEE212 pKa = 5.21PKK214 pKa = 10.14RR215 pKa = 11.84QAPQPPVEE223 pKa = 4.38EE224 pKa = 4.39VVPPAQPPAVNGAQPNVTEE243 pKa = 4.43EE244 pKa = 4.09VAKK247 pKa = 8.95TVCAQPKK254 pKa = 8.57ATMRR258 pKa = 11.84PKK260 pKa = 9.51LTHH263 pKa = 6.62PEE265 pKa = 3.62EE266 pKa = 4.07TADD269 pKa = 4.67YY270 pKa = 10.28IISTNDD276 pKa = 2.75SRR278 pKa = 11.84LVKK281 pKa = 9.95EE282 pKa = 4.37ALGVTPQIVVSSSVDD297 pKa = 3.04QTLRR301 pKa = 11.84WRR303 pKa = 11.84ATKK306 pKa = 9.91IDD308 pKa = 3.74GEE310 pKa = 4.6VVLRR314 pKa = 11.84SEE316 pKa = 4.51PVPVIRR322 pKa = 11.84SDD324 pKa = 3.06AGYY327 pKa = 10.03FSNSGYY333 pKa = 9.94YY334 pKa = 10.35AEE336 pKa = 5.98DD337 pKa = 3.25EE338 pKa = 4.25TRR340 pKa = 11.84PAKK343 pKa = 9.69TWVIGPAAPVRR354 pKa = 11.84LIAWVDD360 pKa = 3.5HH361 pKa = 6.12EE362 pKa = 4.75MLAHH366 pKa = 5.34MRR368 pKa = 11.84RR369 pKa = 11.84YY370 pKa = 9.1IMKK373 pKa = 10.29VGLTQSSYY381 pKa = 11.36HH382 pKa = 6.37KK383 pKa = 10.59LVAQGEE389 pKa = 4.48LFLKK393 pKa = 10.55NFRR396 pKa = 11.84TDD398 pKa = 2.97LTDD401 pKa = 2.86PHH403 pKa = 6.66YY404 pKa = 11.3VNRR407 pKa = 11.84ILDD410 pKa = 3.69STACVAMVPTQNEE423 pKa = 4.22VVNITKK429 pKa = 10.49LSSGLVFNAINTVSDD444 pKa = 3.95FRR446 pKa = 11.84RR447 pKa = 11.84LGLVPRR453 pKa = 11.84KK454 pKa = 9.32VWCGLFNGSPYY465 pKa = 10.64KK466 pKa = 10.74LPSATT471 pKa = 3.73

Molecular weight: 51.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGM9|A0A1L3KGM9_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 19 OX=1923265 PE=4 SV=1
MM1 pKa = 7.31QMPKK5 pKa = 10.09PKK7 pKa = 10.08SKK9 pKa = 9.11KK10 pKa = 6.28TLRR13 pKa = 11.84RR14 pKa = 11.84KK15 pKa = 8.82QPRR18 pKa = 11.84PRR20 pKa = 11.84QQRR23 pKa = 11.84PLAPMNVGARR33 pKa = 11.84LRR35 pKa = 11.84TAQSTRR41 pKa = 11.84KK42 pKa = 9.39KK43 pKa = 10.17PEE45 pKa = 3.71VVTDD49 pKa = 4.2LLLSVDD55 pKa = 3.83IPKK58 pKa = 8.82DD59 pKa = 3.62TPRR62 pKa = 11.84GTILLDD68 pKa = 3.05ISLNPTITKK77 pKa = 10.35RR78 pKa = 11.84LANLSKK84 pKa = 11.13AFVKK88 pKa = 10.02WKK90 pKa = 7.21MTNCEE95 pKa = 4.03IQVQTQVPATMGGGLVGGYY114 pKa = 9.92VPDD117 pKa = 4.05PSIRR121 pKa = 11.84LSDD124 pKa = 4.74GIQVLEE130 pKa = 4.54SLTATNQAKK139 pKa = 9.64IVKK142 pKa = 7.79TWEE145 pKa = 3.99SFTVKK150 pKa = 10.08IPNSKK155 pKa = 9.88EE156 pKa = 3.79LFVVRR161 pKa = 11.84GDD163 pKa = 4.13DD164 pKa = 4.64SRR166 pKa = 11.84LSDD169 pKa = 3.19AGRR172 pKa = 11.84FYY174 pKa = 11.28LVVDD178 pKa = 5.11GPPTDD183 pKa = 3.4NSSIRR188 pKa = 11.84VQIRR192 pKa = 11.84WSVQLLVPFAQDD204 pKa = 2.75EE205 pKa = 4.41RR206 pKa = 11.84NILDD210 pKa = 4.07SLTVTCSALGIQGEE224 pKa = 4.69GVLEE228 pKa = 4.3GSRR231 pKa = 11.84NCTVHH236 pKa = 6.05NWTPQHH242 pKa = 6.09GFAPPFDD249 pKa = 4.01KK250 pKa = 11.15TPDD253 pKa = 3.59LDD255 pKa = 4.36LSHH258 pKa = 6.84GLHH261 pKa = 6.51GLPNFSSAGPNIRR274 pKa = 11.84YY275 pKa = 9.04YY276 pKa = 10.94RR277 pKa = 11.84LDD279 pKa = 3.44QPAIAHH285 pKa = 6.47PAALFLAVFNQRR297 pKa = 11.84MLNASLPDD305 pKa = 3.09SWRR308 pKa = 11.84GVFLDD313 pKa = 3.55MPNYY317 pKa = 9.19PEE319 pKa = 4.96PDD321 pKa = 2.98ISVAPSIMLVQHH333 pKa = 5.66STLTPVLQEE342 pKa = 4.25EE343 pKa = 4.81YY344 pKa = 10.67LGNQEE349 pKa = 3.96GSFLVTVHH357 pKa = 6.84RR358 pKa = 11.84FLSATRR364 pKa = 11.84QLQCSKK370 pKa = 9.82EE371 pKa = 3.88YY372 pKa = 11.07RR373 pKa = 11.84MAQPNPLLTRR383 pKa = 11.84SLPQEE388 pKa = 3.91NVAMVLSRR396 pKa = 11.84KK397 pKa = 8.6LAQMSLVNN405 pKa = 4.04

Molecular weight: 45.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1516

228

471

379.0

42.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.728 ± 0.346

1.451 ± 0.184

4.485 ± 0.472

5.409 ± 0.654

3.826 ± 0.859

5.607 ± 0.309

1.847 ± 0.096

4.485 ± 0.267

5.739 ± 0.686

8.839 ± 0.857

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.441 ± 0.509

4.222 ± 0.426

7.058 ± 0.92

4.551 ± 0.553

7.19 ± 0.348

8.377 ± 1.174

6.069 ± 0.439

7.52 ± 0.173

1.715 ± 0.43

2.441 ± 0.382
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski