Rubrivirga sp. SAORIC476
Average proteome isoelectric point is 5.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3744 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A271JDS6|A0A271JDS6_9BACT Uncharacterized protein OS=Rubrivirga sp. SAORIC476 OX=1961794 GN=B1759_04970 PE=4 SV=1
MM1 pKa = 7.52 HH2 pKa = 7.93 KK3 pKa = 9.93 LTFLLALATALVVLPACDD21 pKa = 3.48 AFGGGDD27 pKa = 3.51 PTIGGTYY34 pKa = 10.17 SGIDD38 pKa = 3.29 PAQGQRR44 pKa = 11.84 VSMIIPADD52 pKa = 3.84 TEE54 pKa = 4.24 SDD56 pKa = 3.42 PDD58 pKa = 3.44 ATFTLTYY65 pKa = 9.77 TEE67 pKa = 4.42 TGFPDD72 pKa = 4.83 AIEE75 pKa = 4.27 TATYY79 pKa = 9.91 AYY81 pKa = 7.44 PTLTLTGDD89 pKa = 3.08 VSLRR93 pKa = 11.84 CTVDD97 pKa = 3.07 EE98 pKa = 4.92 TGDD101 pKa = 3.88 SLDD104 pKa = 4.0 CRR106 pKa = 11.84 PTAGQDD112 pKa = 3.05 FGNFVFTRR120 pKa = 3.72
Molecular weight: 12.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.713
IPC2_protein 3.808
IPC_protein 3.783
Toseland 3.554
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.77
Rodwell 3.605
Grimsley 3.465
Solomon 3.77
Lehninger 3.732
Nozaki 3.923
DTASelect 4.19
Thurlkill 3.63
EMBOSS 3.77
Sillero 3.91
Patrickios 0.846
IPC_peptide 3.77
IPC2_peptide 3.872
IPC2.peptide.svr19 3.803
Protein with the highest isoelectric point:
>tr|A0A271J704|A0A271J704_9BACT Zn_protease domain-containing protein OS=Rubrivirga sp. SAORIC476 OX=1961794 GN=B1759_13300 PE=4 SV=1
MM1 pKa = 7.52 AHH3 pKa = 7.41 RR4 pKa = 11.84 KK5 pKa = 8.03 PHH7 pKa = 6.67 LPTKK11 pKa = 8.61 TCPVCSRR18 pKa = 11.84 PFAWRR23 pKa = 11.84 KK24 pKa = 8.38 KK25 pKa = 6.98 WARR28 pKa = 11.84 DD29 pKa = 3.12 WEE31 pKa = 4.34 AVVYY35 pKa = 10.56 CSEE38 pKa = 3.81 RR39 pKa = 11.84 CRR41 pKa = 11.84 RR42 pKa = 11.84 SKK44 pKa = 10.91 SSAGGPSTGDD54 pKa = 3.26 GARR57 pKa = 11.84 TRR59 pKa = 11.84 LGSTGG64 pKa = 3.16
Molecular weight: 7.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.575
IPC_protein 10.218
Toseland 10.818
ProMoST 10.482
Dawson 10.877
Bjellqvist 10.584
Wikipedia 11.067
Rodwell 11.111
Grimsley 10.906
Solomon 11.023
Lehninger 10.994
Nozaki 10.833
DTASelect 10.57
Thurlkill 10.804
EMBOSS 11.228
Sillero 10.818
Patrickios 10.906
IPC_peptide 11.038
IPC2_peptide 9.926
IPC2.peptide.svr19 8.625
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3744
0
3744
1333258
38
2911
356.1
38.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.523 ± 0.063
0.54 ± 0.01
6.846 ± 0.028
5.757 ± 0.033
3.386 ± 0.024
9.283 ± 0.044
1.799 ± 0.019
3.311 ± 0.03
1.487 ± 0.027
10.279 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.673 ± 0.019
1.881 ± 0.029
5.797 ± 0.032
2.751 ± 0.021
7.722 ± 0.046
5.164 ± 0.031
6.221 ± 0.038
8.885 ± 0.04
1.337 ± 0.017
2.359 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here