Tomato necrotic streak virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Ilarvirus

Average proteome isoelectric point is 7.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140GPI7|A0A140GPI7_9BROM Putative silencing suppressor OS=Tomato necrotic streak virus OX=1637492 GN=2b PE=4 SV=1
MM1 pKa = 7.63EE2 pKa = 6.42IITNVLDD9 pKa = 3.87LVSTVSLRR17 pKa = 11.84TSLQVHH23 pKa = 6.7ASFDD27 pKa = 3.89LPLSIEE33 pKa = 3.85QSFMIRR39 pKa = 11.84LWLVRR44 pKa = 11.84DD45 pKa = 3.79VVIPTMKK52 pKa = 10.43VYY54 pKa = 10.24WDD56 pKa = 3.54ADD58 pKa = 3.44ALLGRR63 pKa = 11.84LEE65 pKa = 4.69TVFSTDD71 pKa = 3.18PEE73 pKa = 4.22PVVMRR78 pKa = 11.84NSTFHH83 pKa = 7.44DD84 pKa = 3.55IVPEE88 pKa = 3.93YY89 pKa = 10.58VDD91 pKa = 3.68EE92 pKa = 4.87FGEE95 pKa = 4.53VPEE98 pKa = 4.18EE99 pKa = 4.43AYY101 pKa = 10.72LSPEE105 pKa = 3.8EE106 pKa = 4.26RR107 pKa = 11.84EE108 pKa = 4.42EE109 pKa = 3.66IHH111 pKa = 6.87KK112 pKa = 9.54ATFLKK117 pKa = 10.33HH118 pKa = 5.15EE119 pKa = 4.29HH120 pKa = 6.52WGSEE124 pKa = 4.02SSGSSFLDD132 pKa = 4.26EE133 pKa = 4.74IEE135 pKa = 4.36CLYY138 pKa = 11.3DD139 pKa = 3.37EE140 pKa = 4.47TVKK143 pKa = 11.12DD144 pKa = 4.68DD145 pKa = 3.88VMQDD149 pKa = 2.74VCTDD153 pKa = 3.26MPKK156 pKa = 10.4DD157 pKa = 3.93YY158 pKa = 11.12EE159 pKa = 4.43IMWNDD164 pKa = 3.55GAVDD168 pKa = 3.98AVWSSRR174 pKa = 11.84FEE176 pKa = 4.02VEE178 pKa = 4.62EE179 pKa = 3.94PPKK182 pKa = 10.62PKK184 pKa = 9.93FRR186 pKa = 11.84PQMTDD191 pKa = 2.89KK192 pKa = 11.21VCDD195 pKa = 3.48PVIIQDD201 pKa = 4.7AINDD205 pKa = 3.88IFPFHH210 pKa = 7.22QDD212 pKa = 2.3MDD214 pKa = 3.76DD215 pKa = 3.76TYY217 pKa = 10.48FQTWVEE223 pKa = 4.15TQDD226 pKa = 3.07ISLEE230 pKa = 4.18VSKK233 pKa = 10.96CWLDD237 pKa = 3.26ASNFKK242 pKa = 10.96DD243 pKa = 3.78FTKK246 pKa = 10.69GQQTYY251 pKa = 10.4AVPTYY256 pKa = 10.83QSGATSKK263 pKa = 10.53RR264 pKa = 11.84VNTQRR269 pKa = 11.84EE270 pKa = 3.87TLLAVKK276 pKa = 9.35KK277 pKa = 10.43RR278 pKa = 11.84NMNIPEE284 pKa = 4.13LQATFDD290 pKa = 4.13LDD292 pKa = 4.62AEE294 pKa = 4.44VATCFKK300 pKa = 10.54RR301 pKa = 11.84FCTHH305 pKa = 5.53VVDD308 pKa = 4.49IPRR311 pKa = 11.84LKK313 pKa = 10.43RR314 pKa = 11.84LPPMMGTEE322 pKa = 4.05TEE324 pKa = 4.2FFHH327 pKa = 7.94RR328 pKa = 11.84LFGWYY333 pKa = 9.7NPPLSEE339 pKa = 4.06YY340 pKa = 10.36RR341 pKa = 11.84GPLTLQSLDD350 pKa = 3.65KK351 pKa = 10.5YY352 pKa = 8.34MHH354 pKa = 5.5MVKK357 pKa = 9.98TIVKK361 pKa = 8.75PVEE364 pKa = 4.43DD365 pKa = 3.85NSLKK369 pKa = 10.61YY370 pKa = 10.06EE371 pKa = 4.23RR372 pKa = 11.84PLCATITLSKK382 pKa = 10.3KK383 pKa = 10.06GIVMQSSPLFLSAMSRR399 pKa = 11.84LFYY402 pKa = 10.61VLKK405 pKa = 10.64SKK407 pKa = 10.19IHH409 pKa = 5.86IPSGKK414 pKa = 7.2WHH416 pKa = 6.3QLFTLDD422 pKa = 3.84ATAFDD427 pKa = 3.62AAQWFKK433 pKa = 11.14EE434 pKa = 3.85VDD436 pKa = 3.72FSKK439 pKa = 10.64FDD441 pKa = 3.3KK442 pKa = 11.19SQGEE446 pKa = 4.19LHH448 pKa = 6.92HH449 pKa = 6.79KK450 pKa = 7.34VQKK453 pKa = 10.9LIFDD457 pKa = 4.63LLKK460 pKa = 10.74LPPEE464 pKa = 4.1FVEE467 pKa = 4.27MWFTSHH473 pKa = 5.89EE474 pKa = 4.18RR475 pKa = 11.84SHH477 pKa = 6.0ITDD480 pKa = 3.49RR481 pKa = 11.84DD482 pKa = 3.68TGVGFSVDD490 pKa = 3.5YY491 pKa = 10.5QRR493 pKa = 11.84RR494 pKa = 11.84TGDD497 pKa = 3.07ANTYY501 pKa = 10.16LGNTLVTLICLARR514 pKa = 11.84VYY516 pKa = 10.32DD517 pKa = 3.84LCDD520 pKa = 3.56PKK522 pKa = 10.23ITFIIASGDD531 pKa = 3.41DD532 pKa = 3.58SLIGSVEE539 pKa = 4.01EE540 pKa = 4.07LPRR543 pKa = 11.84APEE546 pKa = 3.79HH547 pKa = 6.87LFTSLFNFEE556 pKa = 5.0AKK558 pKa = 10.15FPHH561 pKa = 5.93NQPFICSKK569 pKa = 10.53FLVSVDD575 pKa = 3.54LADD578 pKa = 4.2GGRR581 pKa = 11.84EE582 pKa = 4.11VIAVPNPAKK591 pKa = 10.65LLIRR595 pKa = 11.84MGRR598 pKa = 11.84RR599 pKa = 11.84DD600 pKa = 4.04CQFQSLDD607 pKa = 3.57DD608 pKa = 5.61LYY610 pKa = 10.81ISWLDD615 pKa = 3.31VIYY618 pKa = 10.35YY619 pKa = 10.13FRR621 pKa = 11.84DD622 pKa = 3.06SRR624 pKa = 11.84VCEE627 pKa = 4.25KK628 pKa = 10.76VAEE631 pKa = 4.15LCAYY635 pKa = 8.96RR636 pKa = 11.84QTRR639 pKa = 11.84RR640 pKa = 11.84SSMYY644 pKa = 10.48LLSALLSLPSCFANLKK660 pKa = 9.99KK661 pKa = 10.77FKK663 pKa = 10.3LLCYY667 pKa = 10.22NLTEE671 pKa = 4.15EE672 pKa = 4.37EE673 pKa = 4.39CLKK676 pKa = 9.77KK677 pKa = 9.36TKK679 pKa = 9.02LTRR682 pKa = 11.84DD683 pKa = 3.28THH685 pKa = 4.86GHH687 pKa = 4.32VEE689 pKa = 3.86KK690 pKa = 10.24EE691 pKa = 3.78QRR693 pKa = 11.84VNGCRR698 pKa = 11.84NKK700 pKa = 10.98GIVQEE705 pKa = 4.65GTAIPAKK712 pKa = 9.98NGRR715 pKa = 11.84RR716 pKa = 11.84VQEE719 pKa = 3.79TSTRR723 pKa = 11.84PNSGFRR729 pKa = 11.84AYY731 pKa = 9.41PQRR734 pKa = 11.84KK735 pKa = 8.74SRR737 pKa = 11.84GCWIPHH743 pKa = 5.51ISKK746 pKa = 9.88VFQAPDD752 pKa = 3.2QMGGCSCAVTRR763 pKa = 11.84KK764 pKa = 9.24SFSSIRR770 pKa = 11.84SAVDD774 pKa = 2.94VSGFPPAGPPSRR786 pKa = 11.84YY787 pKa = 9.69AEE789 pKa = 4.09SGEE792 pKa = 4.2FGDD795 pKa = 4.24GTRR798 pKa = 11.84TGSSYY803 pKa = 11.09AYY805 pKa = 9.76KK806 pKa = 10.53NSRR809 pKa = 11.84PRR811 pKa = 11.84NRR813 pKa = 11.84LRR815 pKa = 11.84AQGVHH820 pKa = 6.13QSRR823 pKa = 11.84CSNTEE828 pKa = 3.78YY829 pKa = 10.48IPSNNRR835 pKa = 11.84RR836 pKa = 11.84STT838 pKa = 3.23

Molecular weight: 95.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140GPI7|A0A140GPI7_9BROM Putative silencing suppressor OS=Tomato necrotic streak virus OX=1637492 GN=2b PE=4 SV=1
MM1 pKa = 8.12DD2 pKa = 4.34MSRR5 pKa = 11.84KK6 pKa = 10.22SNVSMDD12 pKa = 3.1AVTRR16 pKa = 11.84EE17 pKa = 4.07LFKK20 pKa = 11.19KK21 pKa = 8.86EE22 pKa = 3.41LRR24 pKa = 11.84YY25 pKa = 9.15RR26 pKa = 11.84LKK28 pKa = 10.13MAEE31 pKa = 4.11EE32 pKa = 4.4FKK34 pKa = 10.98KK35 pKa = 10.29QARR38 pKa = 11.84DD39 pKa = 3.75PIVDD43 pKa = 3.74SVHH46 pKa = 4.81THH48 pKa = 5.19SGNRR52 pKa = 11.84VDD54 pKa = 4.67AGYY57 pKa = 10.14HH58 pKa = 5.44ISPKK62 pKa = 9.97FFKK65 pKa = 10.53PLTKK69 pKa = 9.6WGVVPVLSLVNLSAVSEE86 pKa = 4.23ARR88 pKa = 11.84WMLVDD93 pKa = 3.64SHH95 pKa = 6.64QLAPPLVTPRR105 pKa = 11.84VVSSEE110 pKa = 3.93MAPEE114 pKa = 4.03PEE116 pKa = 3.47ARR118 pKa = 11.84MLIKK122 pKa = 10.37IPDD125 pKa = 3.64LGIDD129 pKa = 3.41YY130 pKa = 9.91EE131 pKa = 4.39LRR133 pKa = 11.84EE134 pKa = 4.25FTNPAVVIQSIYY146 pKa = 10.21RR147 pKa = 11.84RR148 pKa = 11.84IIGEE152 pKa = 4.23VPKK155 pKa = 10.48GWYY158 pKa = 9.02GLKK161 pKa = 9.68SWSISSYY168 pKa = 9.18GDD170 pKa = 3.07VYY172 pKa = 11.38SRR174 pKa = 11.84LRR176 pKa = 11.84VVRR179 pKa = 11.84KK180 pKa = 9.06MKK182 pKa = 10.38VHH184 pKa = 5.65LTIPGSDD191 pKa = 2.94WAYY194 pKa = 9.33TLSLSDD200 pKa = 4.38VISGLAIPRR209 pKa = 11.84LPIPEE214 pKa = 5.36KK215 pKa = 9.55YY216 pKa = 10.55LKK218 pKa = 9.73MPISISFRR226 pKa = 11.84DD227 pKa = 3.68EE228 pKa = 3.63MM229 pKa = 6.3

Molecular weight: 26.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2617

213

1046

523.4

59.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.687 ± 0.684

2.025 ± 0.298

6.19 ± 0.297

5.885 ± 0.287

4.241 ± 0.601

4.7 ± 0.327

2.293 ± 0.183

5.464 ± 0.434

6.458 ± 0.227

8.445 ± 0.662

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.981 ± 0.273

3.63 ± 0.419

5.464 ± 0.484

2.828 ± 0.376

6.037 ± 0.305

8.063 ± 0.362

6.305 ± 0.43

7.413 ± 0.454

1.681 ± 0.15

3.21 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski