Sphingobacterium sp. 2c-3

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Sphingobacterium; unclassified Sphingobacterium

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3348 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T8HLL0|A0A2T8HLL0_9SPHI Uncharacterized protein OS=Sphingobacterium sp. 2c-3 OX=2171749 GN=DC487_01430 PE=4 SV=1
MM1 pKa = 8.0RR2 pKa = 11.84SKK4 pKa = 10.6RR5 pKa = 11.84WFLTQIKK12 pKa = 9.9GMKK15 pKa = 9.93DD16 pKa = 2.88LTIINGANQDD26 pKa = 4.28FIFIDD31 pKa = 3.93ADD33 pKa = 3.62SDD35 pKa = 3.74TSGIVAYY42 pKa = 8.81FINSEE47 pKa = 4.37SYY49 pKa = 10.71LDD51 pKa = 5.57GYY53 pKa = 11.34FEE55 pKa = 4.88DD56 pKa = 3.69QDD58 pKa = 4.36LIKK61 pKa = 10.95VEE63 pKa = 4.7VEE65 pKa = 3.91YY66 pKa = 10.66QYY68 pKa = 11.46QALEE72 pKa = 4.3SVSGWEE78 pKa = 3.92YY79 pKa = 10.4PQYY82 pKa = 10.78TDD84 pKa = 3.04TKK86 pKa = 9.81VYY88 pKa = 10.5PGDD91 pKa = 3.38EE92 pKa = 4.17SFDD95 pKa = 3.52YY96 pKa = 11.18DD97 pKa = 3.6SAYY100 pKa = 11.27NEE102 pKa = 3.96ALEE105 pKa = 4.09AAKK108 pKa = 10.44EE109 pKa = 3.89ILLQKK114 pKa = 10.52HH115 pKa = 5.48DD116 pKa = 3.73VQYY119 pKa = 11.7VEE121 pKa = 4.27VV122 pKa = 3.86

Molecular weight: 14.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T8HEZ7|A0A2T8HEZ7_9SPHI DUF4442 domain-containing protein OS=Sphingobacterium sp. 2c-3 OX=2171749 GN=DC487_16175 PE=4 SV=1
MM1 pKa = 7.58YY2 pKa = 8.0YY3 pKa = 10.12TNRR6 pKa = 11.84NNRR9 pKa = 11.84VASSRR14 pKa = 11.84TSGNNFRR21 pKa = 11.84GGSSVAGRR29 pKa = 11.84NTSRR33 pKa = 11.84GVSRR37 pKa = 11.84DD38 pKa = 3.22ANGRR42 pKa = 11.84ILNGTAAGRR51 pKa = 11.84SRR53 pKa = 11.84GATASSGRR61 pKa = 11.84GASSGRR67 pKa = 11.84GTATSPRR74 pKa = 11.84GAGTVGRR81 pKa = 11.84GATTGNRR88 pKa = 11.84GAAGTPSNRR97 pKa = 11.84GGSSTVRR104 pKa = 11.84GAGNPSRR111 pKa = 11.84NTGTVGRR118 pKa = 11.84GATGSTRR125 pKa = 11.84QPVGRR130 pKa = 11.84SQGGSVGRR138 pKa = 11.84SATPRR143 pKa = 11.84STTPPSRR150 pKa = 11.84STINRR155 pKa = 11.84GTSQPTRR162 pKa = 11.84TSSGSSMSRR171 pKa = 11.84GSSGGSMSGGGGGASRR187 pKa = 11.84GGGGGGSRR195 pKa = 11.84GGSSGRR201 pKa = 11.84SRR203 pKa = 3.73

Molecular weight: 19.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3348

0

3348

1168914

38

1809

349.1

39.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.634 ± 0.044

0.69 ± 0.013

5.658 ± 0.032

6.049 ± 0.043

4.833 ± 0.034

6.679 ± 0.039

2.025 ± 0.021

7.114 ± 0.044

6.047 ± 0.038

9.515 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.342 ± 0.016

5.261 ± 0.04

3.636 ± 0.02

4.185 ± 0.026

4.518 ± 0.026

6.453 ± 0.034

5.597 ± 0.028

6.539 ± 0.028

1.183 ± 0.016

4.041 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski