Azomonas agilis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Azotobacter group; Azomonas

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3016 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A562IKR8|A0A562IKR8_9GAMM Uncharacterized protein YjiK OS=Azomonas agilis OX=116849 GN=LX59_01608 PE=3 SV=1
MM1 pKa = 7.95PNPNSPYY8 pKa = 9.89FALASLLGNCPNAITPAEE26 pKa = 4.04LQGVLLGRR34 pKa = 11.84SCAGAGFAPEE44 pKa = 3.66AWLAEE49 pKa = 3.98ACEE52 pKa = 4.13LLGAPAEE59 pKa = 4.47GALQQALLGLQRR71 pKa = 11.84MVQEE75 pKa = 4.6EE76 pKa = 4.9LISTQLALTLLLPEE90 pKa = 5.09DD91 pKa = 4.15EE92 pKa = 5.28APLSVRR98 pKa = 11.84AQALGQWCQGFLDD111 pKa = 5.04GFGQTIGDD119 pKa = 3.52QALSLEE125 pKa = 4.6AVDD128 pKa = 5.22GLQDD132 pKa = 3.63LTSIAQIGDD141 pKa = 3.51QLEE144 pKa = 4.3DD145 pKa = 3.95SEE147 pKa = 6.06DD148 pKa = 3.87SEE150 pKa = 4.41TDD152 pKa = 3.38YY153 pKa = 11.47MEE155 pKa = 3.64VTEE158 pKa = 4.18YY159 pKa = 11.17VRR161 pKa = 11.84VVPLLLFTEE170 pKa = 5.09CGRR173 pKa = 11.84VPAAASSAPVPPVDD187 pKa = 3.93ILQDD191 pKa = 3.1ADD193 pKa = 3.64AASKK197 pKa = 9.09TLHH200 pKa = 6.09

Molecular weight: 21.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A562HZR3|A0A562HZR3_9GAMM 30S ribosomal protein S10 OS=Azomonas agilis OX=116849 GN=rpsJ PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.29NGRR28 pKa = 11.84QVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.83GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3016

0

3016

982890

39

4341

325.9

36.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.064 ± 0.045

1.056 ± 0.015

5.005 ± 0.033

6.035 ± 0.041

3.551 ± 0.027

7.321 ± 0.038

2.401 ± 0.023

5.211 ± 0.031

3.689 ± 0.043

12.058 ± 0.083

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.138 ± 0.019

3.185 ± 0.035

4.912 ± 0.032

5.375 ± 0.05

6.178 ± 0.041

6.025 ± 0.043

5.006 ± 0.037

6.659 ± 0.034

1.431 ± 0.02

2.699 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski