Pipra filicauda (Wire-tailed manakin)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 29436 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7R5K7A6|A0A7R5K7A6_9PASS LOW QUALITY PROTEIN: leucine-rich repeat and death domain-containing protein 1 OS=Pipra filicauda OX=649802 GN=LRRD1 PE=4 SV=1
VV1 pKa = 6.36QLTAEE6 pKa = 4.63FFSCSLHH13 pKa = 5.05STTRR17 pKa = 11.84PSATLTNVLYY27 pKa = 10.37QDD29 pKa = 4.11CFLGQWEE36 pKa = 4.44QNQQHH41 pKa = 6.81PAGNPVVSSAADD53 pKa = 3.33NSVVLLTSDD62 pKa = 4.91DD63 pKa = 3.97EE64 pKa = 4.5EE65 pKa = 4.42EE66 pKa = 4.39PRR68 pKa = 11.84DD69 pKa = 3.77NDD71 pKa = 3.5GTRR74 pKa = 11.84PAAPVTSPTWIDD86 pKa = 4.02DD87 pKa = 3.65YY88 pKa = 12.01SEE90 pKa = 4.39LLEE93 pKa = 5.36GYY95 pKa = 7.46EE96 pKa = 3.88QQRR99 pKa = 11.84RR100 pKa = 11.84NLTPRR105 pKa = 11.84SQQLLDD111 pKa = 3.79SPVLRR116 pKa = 11.84SYY118 pKa = 11.57DD119 pKa = 3.65EE120 pKa = 4.76YY121 pKa = 10.99EE122 pKa = 4.2TADD125 pKa = 3.6DD126 pKa = 3.96VTDD129 pKa = 3.76VEE131 pKa = 5.11SEE133 pKa = 4.24VTDD136 pKa = 3.66VEE138 pKa = 4.88SEE140 pKa = 4.27VTDD143 pKa = 3.66VEE145 pKa = 4.88SEE147 pKa = 4.27VTDD150 pKa = 3.66VEE152 pKa = 4.88SEE154 pKa = 4.27VTDD157 pKa = 3.66VEE159 pKa = 4.88SEE161 pKa = 4.27VTDD164 pKa = 3.66VEE166 pKa = 4.88SEE168 pKa = 4.27VTDD171 pKa = 3.66VEE173 pKa = 4.88SEE175 pKa = 4.27VTDD178 pKa = 3.66VEE180 pKa = 4.88SEE182 pKa = 4.27VTDD185 pKa = 3.66VEE187 pKa = 4.88SEE189 pKa = 4.27VTDD192 pKa = 3.66VEE194 pKa = 4.88SEE196 pKa = 4.27VTDD199 pKa = 3.69VEE201 pKa = 4.65SEE203 pKa = 4.44VIDD206 pKa = 3.82IEE208 pKa = 4.28YY209 pKa = 10.35GAADD213 pKa = 4.14MEE215 pKa = 4.62YY216 pKa = 10.41GAADD220 pKa = 3.4IQYY223 pKa = 8.43EE224 pKa = 4.04AAYY227 pKa = 10.28VEE229 pKa = 4.44PEE231 pKa = 3.82VRR233 pKa = 11.84RR234 pKa = 11.84SLDD237 pKa = 3.24EE238 pKa = 3.86EE239 pKa = 4.28LAISEE244 pKa = 4.09EE245 pKa = 3.85WQRR248 pKa = 11.84AVRR251 pKa = 11.84CPICMDD257 pKa = 4.16FFSQIIRR264 pKa = 11.84SVRR267 pKa = 11.84QVVSTQCGHH276 pKa = 7.04LFCSRR281 pKa = 11.84CIAVALEE288 pKa = 3.89NSRR291 pKa = 11.84ACPTCRR297 pKa = 11.84TEE299 pKa = 4.87LPPRR303 pKa = 11.84DD304 pKa = 3.62YY305 pKa = 11.32HH306 pKa = 6.43PVYY309 pKa = 10.64FF310 pKa = 4.8

Molecular weight: 34.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7R5KR41|A0A7R5KR41_9PASS Isoform of A0A7R5KER5 SH3-containing GRB2-like protein 3-interacting protein 1 isoform X3 OS=Pipra filicauda OX=649802 GN=SGIP1 PE=4 SV=1
MM1 pKa = 7.57SSHH4 pKa = 6.01KK5 pKa = 9.07TFKK8 pKa = 10.28IKK10 pKa = 10.6RR11 pKa = 11.84FLAKK15 pKa = 9.68KK16 pKa = 9.58QKK18 pKa = 8.69QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 9.89TGNKK34 pKa = 8.61IRR36 pKa = 11.84YY37 pKa = 7.09NSKK40 pKa = 8.3RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.95WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16230

13206

29436

21854273

31

32782

742.4

82.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.958 ± 0.013

2.073 ± 0.012

5.014 ± 0.009

7.532 ± 0.022

3.397 ± 0.009

6.217 ± 0.019

2.497 ± 0.006

4.402 ± 0.01

6.102 ± 0.019

9.537 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.182 ± 0.006

3.83 ± 0.009

6.016 ± 0.02

4.94 ± 0.013

5.593 ± 0.013

8.634 ± 0.017

5.357 ± 0.011

6.009 ± 0.011

1.113 ± 0.005

2.594 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski