Clavibacter phage CMP1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cimpunavirus; Clavibacter virus CMP1

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D0U253|D0U253_9CAUD Uncharacterized protein OS=Clavibacter phage CMP1 OX=686439 GN=CMP1-69 PE=4 SV=1
MM1 pKa = 7.75ANQINTKK8 pKa = 10.34DD9 pKa = 3.39AFAEE13 pKa = 4.1LLDD16 pKa = 3.95EE17 pKa = 5.35DD18 pKa = 5.18GPVTVAGIEE27 pKa = 4.15FNPSDD32 pKa = 3.95ILKK35 pKa = 10.11SCDD38 pKa = 3.53PIAFRR43 pKa = 11.84VGISDD48 pKa = 4.15YY49 pKa = 10.33EE50 pKa = 4.37SSMVDD55 pKa = 2.96DD56 pKa = 4.45GTWFEE61 pKa = 4.45WSDD64 pKa = 3.09GDD66 pKa = 5.06HH67 pKa = 6.67YY68 pKa = 11.66DD69 pKa = 3.67EE70 pKa = 5.67EE71 pKa = 4.81EE72 pKa = 4.25PTDD75 pKa = 4.19EE76 pKa = 5.27EE77 pKa = 5.34DD78 pKa = 5.15EE79 pKa = 4.13QDD81 pKa = 3.25TDD83 pKa = 3.84CRR85 pKa = 11.84CGHH88 pKa = 6.95RR89 pKa = 11.84DD90 pKa = 3.67CGACC94 pKa = 4.31

Molecular weight: 10.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D0U258|D0U258_9CAUD Uncharacterized protein OS=Clavibacter phage CMP1 OX=686439 GN=CMP1-74 PE=4 SV=1
MM1 pKa = 6.88STSARR6 pKa = 11.84YY7 pKa = 8.52AATQRR12 pKa = 11.84KK13 pKa = 9.5DD14 pKa = 3.22STHH17 pKa = 5.9VVTVWLPRR25 pKa = 11.84PMTADD30 pKa = 3.26EE31 pKa = 4.96LRR33 pKa = 11.84PKK35 pKa = 9.73IASIRR40 pKa = 11.84RR41 pKa = 11.84YY42 pKa = 9.9AADD45 pKa = 4.18LSRR48 pKa = 11.84AKK50 pKa = 10.54YY51 pKa = 9.37PRR53 pKa = 11.84APKK56 pKa = 9.71PAGLSWRR63 pKa = 11.84DD64 pKa = 3.12AWHH67 pKa = 7.07LAPADD72 pKa = 3.58VAAIEE77 pKa = 4.31AQPGPRR83 pKa = 3.73

Molecular weight: 9.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

17300

53

1551

233.8

25.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.197 ± 0.53

0.497 ± 0.114

6.035 ± 0.178

6.289 ± 0.376

3.168 ± 0.146

8.173 ± 0.312

1.717 ± 0.186

5.324 ± 0.155

4.382 ± 0.182

7.295 ± 0.277

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.59 ± 0.168

4.052 ± 0.168

4.884 ± 0.286

3.584 ± 0.212

6.202 ± 0.284

6.451 ± 0.256

6.803 ± 0.304

6.671 ± 0.267

1.601 ± 0.134

3.087 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski