Amino acid dipepetide frequency for Clavibacter phage CMP1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.18AlaAla: 13.18 ± 1.829
0.347AlaCys: 0.347 ± 0.158
6.821AlaAsp: 6.821 ± 0.56
7.168AlaGlu: 7.168 ± 0.88
3.873AlaPhe: 3.873 ± 0.519
8.96AlaGly: 8.96 ± 0.847
2.081AlaHis: 2.081 ± 0.496
6.763AlaIle: 6.763 ± 0.887
5.318AlaLys: 5.318 ± 0.506
8.382AlaLeu: 8.382 ± 0.942
3.353AlaMet: 3.353 ± 0.415
3.642AlaAsn: 3.642 ± 0.516
4.971AlaPro: 4.971 ± 0.508
5.203AlaGln: 5.203 ± 0.757
7.457AlaArg: 7.457 ± 0.795
5.954AlaSer: 5.954 ± 0.738
7.457AlaThr: 7.457 ± 0.848
6.648AlaVal: 6.648 ± 0.765
1.676AlaTrp: 1.676 ± 0.346
2.717AlaTyr: 2.717 ± 0.393
0.0AlaXaa: 0.0 ± 0.0
Cys
0.578CysAla: 0.578 ± 0.223
0.058CysCys: 0.058 ± 0.056
0.347CysAsp: 0.347 ± 0.138
0.347CysGlu: 0.347 ± 0.164
0.0CysPhe: 0.0 ± 0.0
0.52CysGly: 0.52 ± 0.189
0.058CysHis: 0.058 ± 0.058
0.173CysIle: 0.173 ± 0.103
0.231CysLys: 0.231 ± 0.147
0.52CysLeu: 0.52 ± 0.242
0.058CysMet: 0.058 ± 0.062
0.173CysAsn: 0.173 ± 0.102
0.347CysPro: 0.347 ± 0.15
0.231CysGln: 0.231 ± 0.11
0.462CysArg: 0.462 ± 0.179
0.231CysSer: 0.231 ± 0.129
0.116CysThr: 0.116 ± 0.086
0.462CysVal: 0.462 ± 0.144
0.058CysTrp: 0.058 ± 0.059
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
6.648AspAla: 6.648 ± 0.773
0.405AspCys: 0.405 ± 0.174
3.237AspAsp: 3.237 ± 0.323
4.336AspGlu: 4.336 ± 0.586
2.254AspPhe: 2.254 ± 0.38
5.723AspGly: 5.723 ± 0.73
1.098AspHis: 1.098 ± 0.267
2.659AspIle: 2.659 ± 0.378
2.89AspLys: 2.89 ± 0.461
4.567AspLeu: 4.567 ± 0.53
1.561AspMet: 1.561 ± 0.288
2.601AspAsn: 2.601 ± 0.35
3.179AspPro: 3.179 ± 0.433
1.965AspGln: 1.965 ± 0.331
2.833AspArg: 2.833 ± 0.446
3.642AspSer: 3.642 ± 0.548
3.7AspThr: 3.7 ± 0.468
4.162AspVal: 4.162 ± 0.455
0.867AspTrp: 0.867 ± 0.22
2.023AspTyr: 2.023 ± 0.38
0.0AspXaa: 0.0 ± 0.0
Glu
7.573GluAla: 7.573 ± 0.917
0.347GluCys: 0.347 ± 0.135
3.179GluAsp: 3.179 ± 0.598
4.046GluGlu: 4.046 ± 0.593
2.254GluPhe: 2.254 ± 0.338
4.336GluGly: 4.336 ± 0.596
1.734GluHis: 1.734 ± 0.353
3.873GluIle: 3.873 ± 0.576
3.179GluLys: 3.179 ± 0.409
5.376GluLeu: 5.376 ± 0.485
1.619GluMet: 1.619 ± 0.309
2.254GluAsn: 2.254 ± 0.385
2.833GluPro: 2.833 ± 0.546
2.139GluGln: 2.139 ± 0.401
3.989GluArg: 3.989 ± 0.499
3.931GluSer: 3.931 ± 0.573
3.873GluThr: 3.873 ± 0.499
3.353GluVal: 3.353 ± 0.576
1.445GluTrp: 1.445 ± 0.386
1.561GluTyr: 1.561 ± 0.39
0.0GluXaa: 0.0 ± 0.0
Phe
3.122PheAla: 3.122 ± 0.338
0.116PheCys: 0.116 ± 0.074
2.254PheAsp: 2.254 ± 0.313
1.734PheGlu: 1.734 ± 0.275
0.694PhePhe: 0.694 ± 0.211
2.948PheGly: 2.948 ± 0.432
0.52PheHis: 0.52 ± 0.203
1.156PheIle: 1.156 ± 0.237
1.33PheLys: 1.33 ± 0.297
2.312PheLeu: 2.312 ± 0.422
0.809PheMet: 0.809 ± 0.198
2.254PheAsn: 2.254 ± 0.45
1.445PhePro: 1.445 ± 0.308
1.156PheGln: 1.156 ± 0.274
1.676PheArg: 1.676 ± 0.348
2.428PheSer: 2.428 ± 0.419
2.312PheThr: 2.312 ± 0.394
2.139PheVal: 2.139 ± 0.424
0.578PheTrp: 0.578 ± 0.188
0.694PheTyr: 0.694 ± 0.2
0.0PheXaa: 0.0 ± 0.0
Gly
8.151GlyAla: 8.151 ± 0.889
0.405GlyCys: 0.405 ± 0.187
4.798GlyAsp: 4.798 ± 0.398
4.451GlyGlu: 4.451 ± 0.562
2.486GlyPhe: 2.486 ± 0.282
7.977GlyGly: 7.977 ± 1.075
1.214GlyHis: 1.214 ± 0.291
5.318GlyIle: 5.318 ± 0.447
3.873GlyLys: 3.873 ± 0.508
6.243GlyLeu: 6.243 ± 0.957
2.081GlyMet: 2.081 ± 0.319
2.775GlyAsn: 2.775 ± 0.335
2.197GlyPro: 2.197 ± 0.304
2.775GlyGln: 2.775 ± 0.479
4.682GlyArg: 4.682 ± 0.672
5.087GlySer: 5.087 ± 0.534
6.128GlyThr: 6.128 ± 0.781
5.723GlyVal: 5.723 ± 0.653
1.503GlyTrp: 1.503 ± 0.353
3.873GlyTyr: 3.873 ± 0.621
0.0GlyXaa: 0.0 ± 0.0
His
1.908HisAla: 1.908 ± 0.366
0.173HisCys: 0.173 ± 0.102
1.445HisAsp: 1.445 ± 0.295
1.676HisGlu: 1.676 ± 0.356
0.405HisPhe: 0.405 ± 0.174
1.445HisGly: 1.445 ± 0.35
0.116HisHis: 0.116 ± 0.084
1.041HisIle: 1.041 ± 0.264
0.347HisLys: 0.347 ± 0.124
1.214HisLeu: 1.214 ± 0.288
0.347HisMet: 0.347 ± 0.134
0.809HisAsn: 0.809 ± 0.32
0.809HisPro: 0.809 ± 0.216
0.462HisGln: 0.462 ± 0.182
1.098HisArg: 1.098 ± 0.294
0.925HisSer: 0.925 ± 0.21
0.867HisThr: 0.867 ± 0.277
0.983HisVal: 0.983 ± 0.213
0.289HisTrp: 0.289 ± 0.135
0.809HisTyr: 0.809 ± 0.273
0.0HisXaa: 0.0 ± 0.0
Ile
5.954IleAla: 5.954 ± 0.47
0.231IleCys: 0.231 ± 0.126
3.411IleAsp: 3.411 ± 0.389
4.278IleGlu: 4.278 ± 0.558
0.925IlePhe: 0.925 ± 0.23
3.642IleGly: 3.642 ± 0.368
1.272IleHis: 1.272 ± 0.302
1.503IleIle: 1.503 ± 0.334
1.908IleLys: 1.908 ± 0.31
3.179IleLeu: 3.179 ± 0.44
1.041IleMet: 1.041 ± 0.249
2.312IleAsn: 2.312 ± 0.43
3.7IlePro: 3.7 ± 0.439
2.486IleGln: 2.486 ± 0.379
3.584IleArg: 3.584 ± 0.493
4.104IleSer: 4.104 ± 0.495
3.757IleThr: 3.757 ± 0.539
3.526IleVal: 3.526 ± 0.554
0.52IleTrp: 0.52 ± 0.225
1.908IleTyr: 1.908 ± 0.291
0.0IleXaa: 0.0 ± 0.0
Lys
4.509LysAla: 4.509 ± 0.657
0.289LysCys: 0.289 ± 0.16
2.37LysAsp: 2.37 ± 0.341
2.312LysGlu: 2.312 ± 0.342
1.561LysPhe: 1.561 ± 0.263
2.948LysGly: 2.948 ± 0.315
0.694LysHis: 0.694 ± 0.249
2.312LysIle: 2.312 ± 0.349
2.197LysLys: 2.197 ± 0.371
2.543LysLeu: 2.543 ± 0.392
1.676LysMet: 1.676 ± 0.434
1.734LysAsn: 1.734 ± 0.328
2.659LysPro: 2.659 ± 0.467
1.503LysGln: 1.503 ± 0.353
3.122LysArg: 3.122 ± 0.488
3.295LysSer: 3.295 ± 0.432
3.122LysThr: 3.122 ± 0.434
2.89LysVal: 2.89 ± 0.549
0.694LysTrp: 0.694 ± 0.175
1.387LysTyr: 1.387 ± 0.289
0.0LysXaa: 0.0 ± 0.0
Leu
8.671LeuAla: 8.671 ± 0.86
0.347LeuCys: 0.347 ± 0.157
4.914LeuAsp: 4.914 ± 0.508
4.509LeuGlu: 4.509 ± 0.724
1.908LeuPhe: 1.908 ± 0.338
5.376LeuGly: 5.376 ± 0.83
1.098LeuHis: 1.098 ± 0.295
3.006LeuIle: 3.006 ± 0.378
3.989LeuLys: 3.989 ± 0.472
4.625LeuLeu: 4.625 ± 0.556
2.139LeuMet: 2.139 ± 0.374
3.179LeuAsn: 3.179 ± 0.512
3.815LeuPro: 3.815 ± 0.435
2.312LeuGln: 2.312 ± 0.402
5.434LeuArg: 5.434 ± 0.629
5.607LeuSer: 5.607 ± 0.733
5.087LeuThr: 5.087 ± 0.519
4.856LeuVal: 4.856 ± 0.409
0.578LeuTrp: 0.578 ± 0.208
1.503LeuTyr: 1.503 ± 0.288
0.0LeuXaa: 0.0 ± 0.0
Met
2.601MetAla: 2.601 ± 0.295
0.173MetCys: 0.173 ± 0.122
1.676MetAsp: 1.676 ± 0.309
1.387MetGlu: 1.387 ± 0.303
0.751MetPhe: 0.751 ± 0.188
1.561MetGly: 1.561 ± 0.301
0.405MetHis: 0.405 ± 0.173
1.041MetIle: 1.041 ± 0.226
1.098MetLys: 1.098 ± 0.25
2.139MetLeu: 2.139 ± 0.248
0.636MetMet: 0.636 ± 0.261
1.214MetAsn: 1.214 ± 0.234
1.503MetPro: 1.503 ± 0.284
1.214MetGln: 1.214 ± 0.395
1.85MetArg: 1.85 ± 0.382
2.139MetSer: 2.139 ± 0.319
2.197MetThr: 2.197 ± 0.399
1.387MetVal: 1.387 ± 0.31
0.289MetTrp: 0.289 ± 0.136
0.636MetTyr: 0.636 ± 0.193
0.0MetXaa: 0.0 ± 0.0
Asn
4.393AsnAla: 4.393 ± 0.668
0.173AsnCys: 0.173 ± 0.101
2.312AsnAsp: 2.312 ± 0.309
1.561AsnGlu: 1.561 ± 0.329
1.387AsnPhe: 1.387 ± 0.296
3.526AsnGly: 3.526 ± 0.457
0.867AsnHis: 0.867 ± 0.224
2.254AsnIle: 2.254 ± 0.372
1.792AsnLys: 1.792 ± 0.278
3.642AsnLeu: 3.642 ± 0.396
0.925AsnMet: 0.925 ± 0.198
1.156AsnAsn: 1.156 ± 0.334
2.37AsnPro: 2.37 ± 0.307
1.387AsnGln: 1.387 ± 0.332
2.37AsnArg: 2.37 ± 0.562
2.833AsnSer: 2.833 ± 0.48
3.064AsnThr: 3.064 ± 0.485
2.428AsnVal: 2.428 ± 0.305
0.867AsnTrp: 0.867 ± 0.244
1.214AsnTyr: 1.214 ± 0.285
0.0AsnXaa: 0.0 ± 0.0
Pro
5.318ProAla: 5.318 ± 0.604
0.116ProCys: 0.116 ± 0.066
3.353ProAsp: 3.353 ± 0.557
4.104ProGlu: 4.104 ± 0.48
1.561ProPhe: 1.561 ± 0.287
4.104ProGly: 4.104 ± 0.499
0.809ProHis: 0.809 ± 0.221
2.254ProIle: 2.254 ± 0.275
2.543ProLys: 2.543 ± 0.483
2.717ProLeu: 2.717 ± 0.464
1.156ProMet: 1.156 ± 0.319
2.081ProAsn: 2.081 ± 0.377
2.254ProPro: 2.254 ± 0.48
1.041ProGln: 1.041 ± 0.246
2.543ProArg: 2.543 ± 0.462
3.353ProSer: 3.353 ± 0.478
3.584ProThr: 3.584 ± 0.625
4.046ProVal: 4.046 ± 0.637
0.462ProTrp: 0.462 ± 0.123
1.445ProTyr: 1.445 ± 0.291
0.0ProXaa: 0.0 ± 0.0
Gln
4.162GlnAla: 4.162 ± 0.86
0.058GlnCys: 0.058 ± 0.063
1.965GlnAsp: 1.965 ± 0.357
1.734GlnGlu: 1.734 ± 0.386
1.214GlnPhe: 1.214 ± 0.25
3.064GlnGly: 3.064 ± 0.455
0.289GlnHis: 0.289 ± 0.121
2.197GlnIle: 2.197 ± 0.438
1.33GlnLys: 1.33 ± 0.294
3.411GlnLeu: 3.411 ± 0.585
1.214GlnMet: 1.214 ± 0.254
1.619GlnAsn: 1.619 ± 0.298
1.792GlnPro: 1.792 ± 0.3
1.85GlnGln: 1.85 ± 0.472
2.254GlnArg: 2.254 ± 0.354
1.85GlnSer: 1.85 ± 0.333
1.965GlnThr: 1.965 ± 0.365
2.37GlnVal: 2.37 ± 0.391
0.405GlnTrp: 0.405 ± 0.137
1.098GlnTyr: 1.098 ± 0.254
0.0GlnXaa: 0.0 ± 0.0
Arg
6.243ArgAla: 6.243 ± 0.883
0.289ArgCys: 0.289 ± 0.133
3.526ArgAsp: 3.526 ± 0.535
3.584ArgGlu: 3.584 ± 0.444
2.543ArgPhe: 2.543 ± 0.353
4.278ArgGly: 4.278 ± 0.533
0.925ArgHis: 0.925 ± 0.292
4.393ArgIle: 4.393 ± 0.639
2.717ArgLys: 2.717 ± 0.414
4.971ArgLeu: 4.971 ± 0.538
1.965ArgMet: 1.965 ± 0.353
2.543ArgAsn: 2.543 ± 0.455
2.659ArgPro: 2.659 ± 0.445
2.312ArgGln: 2.312 ± 0.426
3.931ArgArg: 3.931 ± 0.528
3.295ArgSer: 3.295 ± 0.406
3.757ArgThr: 3.757 ± 0.453
4.393ArgVal: 4.393 ± 0.516
1.503ArgTrp: 1.503 ± 0.302
2.197ArgTyr: 2.197 ± 0.336
0.0ArgXaa: 0.0 ± 0.0
Ser
8.209SerAla: 8.209 ± 1.13
0.347SerCys: 0.347 ± 0.129
3.122SerAsp: 3.122 ± 0.398
4.046SerGlu: 4.046 ± 0.407
1.908SerPhe: 1.908 ± 0.344
5.954SerGly: 5.954 ± 0.722
0.867SerHis: 0.867 ± 0.243
3.237SerIle: 3.237 ± 0.413
2.197SerLys: 2.197 ± 0.421
4.798SerLeu: 4.798 ± 0.545
0.925SerMet: 0.925 ± 0.23
2.775SerAsn: 2.775 ± 0.408
2.659SerPro: 2.659 ± 0.514
1.619SerGln: 1.619 ± 0.3
4.567SerArg: 4.567 ± 0.576
4.74SerSer: 4.74 ± 0.487
3.989SerThr: 3.989 ± 0.446
5.029SerVal: 5.029 ± 0.626
1.33SerTrp: 1.33 ± 0.254
2.139SerTyr: 2.139 ± 0.266
0.0SerXaa: 0.0 ± 0.0
Thr
7.862ThrAla: 7.862 ± 0.794
0.405ThrCys: 0.405 ± 0.174
4.682ThrAsp: 4.682 ± 0.556
4.682ThrGlu: 4.682 ± 0.545
2.254ThrPhe: 2.254 ± 0.389
6.243ThrGly: 6.243 ± 0.639
1.33ThrHis: 1.33 ± 0.329
3.989ThrIle: 3.989 ± 0.537
3.122ThrLys: 3.122 ± 0.379
4.336ThrLeu: 4.336 ± 0.481
1.33ThrMet: 1.33 ± 0.299
1.85ThrAsn: 1.85 ± 0.278
3.989ThrPro: 3.989 ± 0.7
2.081ThrGln: 2.081 ± 0.376
3.295ThrArg: 3.295 ± 0.45
4.451ThrSer: 4.451 ± 0.623
4.798ThrThr: 4.798 ± 0.719
4.625ThrVal: 4.625 ± 0.572
1.156ThrTrp: 1.156 ± 0.236
1.561ThrTyr: 1.561 ± 0.348
0.0ThrXaa: 0.0 ± 0.0
Val
8.266ValAla: 8.266 ± 0.801
0.289ValCys: 0.289 ± 0.118
4.22ValAsp: 4.22 ± 0.487
3.931ValGlu: 3.931 ± 0.597
1.908ValPhe: 1.908 ± 0.35
5.723ValGly: 5.723 ± 0.546
0.867ValHis: 0.867 ± 0.25
3.468ValIle: 3.468 ± 0.502
2.254ValLys: 2.254 ± 0.401
4.971ValLeu: 4.971 ± 0.47
1.734ValMet: 1.734 ± 0.369
2.717ValAsn: 2.717 ± 0.415
3.526ValPro: 3.526 ± 0.444
2.023ValGln: 2.023 ± 0.296
3.757ValArg: 3.757 ± 0.523
4.22ValSer: 4.22 ± 0.574
5.26ValThr: 5.26 ± 0.586
4.451ValVal: 4.451 ± 0.754
0.867ValTrp: 0.867 ± 0.291
2.254ValTyr: 2.254 ± 0.451
0.0ValXaa: 0.0 ± 0.0
Trp
1.561TrpAla: 1.561 ± 0.321
0.231TrpCys: 0.231 ± 0.155
0.867TrpAsp: 0.867 ± 0.216
0.867TrpGlu: 0.867 ± 0.232
0.809TrpPhe: 0.809 ± 0.232
1.098TrpGly: 1.098 ± 0.242
0.578TrpHis: 0.578 ± 0.181
1.041TrpIle: 1.041 ± 0.236
0.694TrpLys: 0.694 ± 0.195
0.983TrpLeu: 0.983 ± 0.242
0.347TrpMet: 0.347 ± 0.123
0.983TrpAsn: 0.983 ± 0.194
0.347TrpPro: 0.347 ± 0.138
0.578TrpGln: 0.578 ± 0.192
1.214TrpArg: 1.214 ± 0.287
0.694TrpSer: 0.694 ± 0.237
1.098TrpThr: 1.098 ± 0.234
1.156TrpVal: 1.156 ± 0.289
0.289TrpTrp: 0.289 ± 0.144
0.578TrpTyr: 0.578 ± 0.168
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.064TyrAla: 3.064 ± 0.409
0.173TyrCys: 0.173 ± 0.145
1.85TyrAsp: 1.85 ± 0.285
2.139TyrGlu: 2.139 ± 0.357
0.983TyrPhe: 0.983 ± 0.212
2.312TyrGly: 2.312 ± 0.409
0.347TyrHis: 0.347 ± 0.124
1.561TyrIle: 1.561 ± 0.328
0.809TyrLys: 0.809 ± 0.205
2.023TyrLeu: 2.023 ± 0.388
0.925TyrMet: 0.925 ± 0.29
1.85TyrAsn: 1.85 ± 0.283
1.792TyrPro: 1.792 ± 0.325
1.503TyrGln: 1.503 ± 0.297
1.908TyrArg: 1.908 ± 0.267
1.734TyrSer: 1.734 ± 0.4
1.908TyrThr: 1.908 ± 0.288
2.081TyrVal: 2.081 ± 0.332
0.636TyrTrp: 0.636 ± 0.169
1.272TyrTyr: 1.272 ± 0.271
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 74 proteins (17300 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski