Halomonas sp. SUBG004

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas; unclassified Halomonas

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2023 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A085DX94|A0A085DX94_9GAMM Threonylcarbamoyl-AMP synthase OS=Halomonas sp. SUBG004 OX=1485007 GN=tsaC PE=3 SV=1
MM1 pKa = 6.89VWQVEE6 pKa = 4.11DD7 pKa = 4.63SIGNLLNGISNVNAGDD23 pKa = 3.6TAFGDD28 pKa = 3.94DD29 pKa = 4.97AIVNAQTVRR38 pKa = 11.84VTGTTITQAQYY49 pKa = 11.17EE50 pKa = 4.44EE51 pKa = 4.54LLAAVGDD58 pKa = 4.17ALSLEE63 pKa = 4.43AGEE66 pKa = 4.18NAARR70 pKa = 11.84TTVTYY75 pKa = 10.96ADD77 pKa = 3.77LAAAVSAQANGVLAPSDD94 pKa = 4.27FYY96 pKa = 11.62SITANTTYY104 pKa = 10.84TNTNGALSVANALTEE119 pKa = 3.96IEE121 pKa = 4.44TVKK124 pKa = 10.9SILAGARR131 pKa = 11.84NTLNFEE137 pKa = 4.5TVYY140 pKa = 9.97EE141 pKa = 4.0WSITDD146 pKa = 3.53SASNILDD153 pKa = 4.89AITTAVPEE161 pKa = 4.15VLEE164 pKa = 4.32DD165 pKa = 3.6AEE167 pKa = 4.54NIRR170 pKa = 11.84VASGGAGDD178 pKa = 4.81NILAAEE184 pKa = 4.14YY185 pKa = 10.04DD186 pKa = 3.78ALVDD190 pKa = 3.87GLGTIPFDD198 pKa = 4.27YY199 pKa = 11.41NEE201 pKa = 4.01VAYY204 pKa = 9.23TLAYY208 pKa = 10.7VFDD211 pKa = 3.98EE212 pKa = 4.56TNDD215 pKa = 3.31TFAAPDD221 pKa = 3.73DD222 pKa = 3.92LADD225 pKa = 3.82RR226 pKa = 11.84FFFTSDD232 pKa = 2.86VFEE235 pKa = 5.66INDD238 pKa = 3.72ALGWGSMLPRR248 pKa = 11.84QKK250 pKa = 10.66SITMPSRR257 pKa = 11.84RR258 pKa = 11.84WWMQAPTICSSPISTRR274 pKa = 11.84GASWIVRR281 pKa = 11.84PILSMHH287 pKa = 5.68TTATAIGTPPYY298 pKa = 10.73VSDD301 pKa = 3.52ATLVEE306 pKa = 4.39INDD309 pKa = 3.84TAVITFEE316 pKa = 3.94QYY318 pKa = 11.1GILEE322 pKa = 4.28QIPGFEE328 pKa = 4.0IQSYY332 pKa = 6.8TLEE335 pKa = 4.51DD336 pKa = 3.08AVGIYY341 pKa = 10.83LEE343 pKa = 5.24DD344 pKa = 3.68NSGLATNYY352 pKa = 10.09IIDD355 pKa = 3.89TEE357 pKa = 4.25QPYY360 pKa = 9.58QAQKK364 pKa = 11.02SLGRR368 pKa = 11.84RR369 pKa = 11.84CFDD372 pKa = 3.51LL373 pKa = 5.86

Molecular weight: 40.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A085DXN7|A0A085DXN7_9GAMM Glutamate racemase OS=Halomonas sp. SUBG004 OX=1485007 GN=murI PE=3 SV=1
MM1 pKa = 7.2SVVGSTLIGGAIGAWRR17 pKa = 11.84ATAVTGPLPLAVTRR31 pKa = 11.84FPLAKK36 pKa = 10.17LPAAMMGGRR45 pKa = 11.84GRR47 pKa = 11.84WPNRR51 pKa = 11.84LAHH54 pKa = 5.9GHH56 pKa = 6.82LIVV59 pKa = 4.32

Molecular weight: 6.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2023

0

2023

363457

30

1032

179.7

19.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.968 ± 0.063

1.058 ± 0.02

5.339 ± 0.052

6.106 ± 0.063

3.492 ± 0.04

7.815 ± 0.06

2.538 ± 0.035

4.675 ± 0.045

2.885 ± 0.05

11.207 ± 0.097

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.787 ± 0.031

2.813 ± 0.037

4.858 ± 0.045

4.54 ± 0.057

6.519 ± 0.061

5.912 ± 0.045

5.353 ± 0.043

7.177 ± 0.055

1.551 ± 0.03

2.395 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski