Amino acid dipepetide frequency for Halomonas sp. SUBG004

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.026AlaAla: 12.026 ± 0.202
1.354AlaCys: 1.354 ± 0.074
5.555AlaAsp: 5.555 ± 0.128
6.716AlaGlu: 6.716 ± 0.157
3.885AlaPhe: 3.885 ± 0.097
8.114AlaGly: 8.114 ± 0.179
2.446AlaHis: 2.446 ± 0.08
5.244AlaIle: 5.244 ± 0.122
3.304AlaLys: 3.304 ± 0.097
14.205AlaLeu: 14.205 ± 0.222
3.45AlaMet: 3.45 ± 0.106
3.247AlaAsn: 3.247 ± 0.095
4.842AlaPro: 4.842 ± 0.118
4.886AlaGln: 4.886 ± 0.142
6.79AlaArg: 6.79 ± 0.146
6.614AlaSer: 6.614 ± 0.116
5.583AlaThr: 5.583 ± 0.13
7.145AlaVal: 7.145 ± 0.159
1.907AlaTrp: 1.907 ± 0.077
2.358AlaTyr: 2.358 ± 0.083
0.008AlaXaa: 0.008 ± 0.005
Cys
1.037CysAla: 1.037 ± 0.058
0.19CysCys: 0.19 ± 0.025
0.603CysAsp: 0.603 ± 0.041
0.515CysGlu: 0.515 ± 0.04
0.429CysPhe: 0.429 ± 0.038
0.988CysGly: 0.988 ± 0.056
0.382CysHis: 0.382 ± 0.035
0.402CysIle: 0.402 ± 0.036
0.22CysLys: 0.22 ± 0.027
1.164CysLeu: 1.164 ± 0.057
0.239CysMet: 0.239 ± 0.025
0.223CysAsn: 0.223 ± 0.027
0.603CysPro: 0.603 ± 0.042
0.528CysGln: 0.528 ± 0.039
0.715CysArg: 0.715 ± 0.047
0.608CysSer: 0.608 ± 0.036
0.426CysThr: 0.426 ± 0.035
0.792CysVal: 0.792 ± 0.04
0.223CysTrp: 0.223 ± 0.027
0.289CysTyr: 0.289 ± 0.034
0.0CysXaa: 0.0 ± 0.0
Asp
6.512AspAla: 6.512 ± 0.143
0.429AspCys: 0.429 ± 0.043
3.456AspAsp: 3.456 ± 0.104
3.745AspGlu: 3.745 ± 0.118
1.728AspPhe: 1.728 ± 0.076
4.122AspGly: 4.122 ± 0.114
1.45AspHis: 1.45 ± 0.063
2.963AspIle: 2.963 ± 0.094
1.411AspLys: 1.411 ± 0.055
4.688AspLeu: 4.688 ± 0.113
1.323AspMet: 1.323 ± 0.057
1.61AspAsn: 1.61 ± 0.073
2.493AspPro: 2.493 ± 0.081
2.121AspGln: 2.121 ± 0.079
2.85AspArg: 2.85 ± 0.094
2.581AspSer: 2.581 ± 0.088
3.205AspThr: 3.205 ± 0.086
4.276AspVal: 4.276 ± 0.109
0.825AspTrp: 0.825 ± 0.05
1.593AspTyr: 1.593 ± 0.071
0.006AspXaa: 0.006 ± 0.004
Glu
7.181GluAla: 7.181 ± 0.153
0.429GluCys: 0.429 ± 0.032
2.534GluAsp: 2.534 ± 0.083
4.166GluGlu: 4.166 ± 0.128
1.566GluPhe: 1.566 ± 0.065
4.617GluGly: 4.617 ± 0.117
1.761GluHis: 1.761 ± 0.073
2.716GluIle: 2.716 ± 0.097
1.912GluLys: 1.912 ± 0.076
6.427GluLeu: 6.427 ± 0.143
1.843GluMet: 1.843 ± 0.071
1.629GluAsn: 1.629 ± 0.07
2.517GluPro: 2.517 ± 0.092
3.923GluGln: 3.923 ± 0.123
5.307GluArg: 5.307 ± 0.123
3.236GluSer: 3.236 ± 0.083
3.06GluThr: 3.06 ± 0.091
4.111GluVal: 4.111 ± 0.116
0.927GluTrp: 0.927 ± 0.046
1.194GluTyr: 1.194 ± 0.068
0.003GluXaa: 0.003 ± 0.003
Phe
3.541PheAla: 3.541 ± 0.11
0.44PheCys: 0.44 ± 0.037
2.325PheAsp: 2.325 ± 0.077
2.152PheGlu: 2.152 ± 0.08
1.538PhePhe: 1.538 ± 0.075
3.164PheGly: 3.164 ± 0.1
0.806PheHis: 0.806 ± 0.054
1.841PheIle: 1.841 ± 0.082
1.013PheLys: 1.013 ± 0.056
2.963PheLeu: 2.963 ± 0.103
0.935PheMet: 0.935 ± 0.054
1.079PheAsn: 1.079 ± 0.054
1.403PhePro: 1.403 ± 0.063
1.378PheGln: 1.378 ± 0.058
1.744PheArg: 1.744 ± 0.066
2.551PheSer: 2.551 ± 0.078
2.105PheThr: 2.105 ± 0.074
2.416PheVal: 2.416 ± 0.095
0.548PheTrp: 0.548 ± 0.045
0.974PheTyr: 0.974 ± 0.049
0.008PheXaa: 0.008 ± 0.005
Gly
6.911GlyAla: 6.911 ± 0.161
1.109GlyCys: 1.109 ± 0.051
4.144GlyAsp: 4.144 ± 0.123
4.95GlyGlu: 4.95 ± 0.123
3.117GlyPhe: 3.117 ± 0.106
6.4GlyGly: 6.4 ± 0.146
2.124GlyHis: 2.124 ± 0.078
4.243GlyIle: 4.243 ± 0.101
2.922GlyLys: 2.922 ± 0.105
8.656GlyLeu: 8.656 ± 0.167
2.523GlyMet: 2.523 ± 0.088
2.226GlyAsn: 2.226 ± 0.082
2.562GlyPro: 2.562 ± 0.072
3.406GlyGln: 3.406 ± 0.107
4.691GlyArg: 4.691 ± 0.122
4.438GlySer: 4.438 ± 0.123
3.802GlyThr: 3.802 ± 0.114
6.177GlyVal: 6.177 ± 0.155
1.34GlyTrp: 1.34 ± 0.062
2.391GlyTyr: 2.391 ± 0.076
0.022GlyXaa: 0.022 ± 0.008
His
2.751HisAla: 2.751 ± 0.088
0.36HisCys: 0.36 ± 0.03
1.304HisAsp: 1.304 ± 0.066
1.194HisGlu: 1.194 ± 0.06
0.938HisPhe: 0.938 ± 0.042
2.306HisGly: 2.306 ± 0.088
0.911HisHis: 0.911 ± 0.053
1.098HisIle: 1.098 ± 0.058
0.487HisLys: 0.487 ± 0.033
2.746HisLeu: 2.746 ± 0.1
0.526HisMet: 0.526 ± 0.035
0.578HisAsn: 0.578 ± 0.042
1.566HisPro: 1.566 ± 0.068
1.249HisGln: 1.249 ± 0.065
1.791HisArg: 1.791 ± 0.074
1.321HisSer: 1.321 ± 0.059
1.235HisThr: 1.235 ± 0.058
1.626HisVal: 1.626 ± 0.072
0.517HisTrp: 0.517 ± 0.036
0.867HisTyr: 0.867 ± 0.046
0.006HisXaa: 0.006 ± 0.004
Ile
5.781IleAla: 5.781 ± 0.129
0.476IleCys: 0.476 ± 0.038
3.376IleAsp: 3.376 ± 0.094
3.604IleGlu: 3.604 ± 0.104
1.315IlePhe: 1.315 ± 0.067
3.987IleGly: 3.987 ± 0.116
1.101IleHis: 1.101 ± 0.054
2.11IleIle: 2.11 ± 0.089
1.387IleLys: 1.387 ± 0.062
3.676IleLeu: 3.676 ± 0.108
1.037IleMet: 1.037 ± 0.051
1.728IleAsn: 1.728 ± 0.061
2.006IlePro: 2.006 ± 0.069
1.582IleGln: 1.582 ± 0.059
2.707IleArg: 2.707 ± 0.082
2.971IleSer: 2.971 ± 0.087
3.06IleThr: 3.06 ± 0.101
3.313IleVal: 3.313 ± 0.102
0.421IleTrp: 0.421 ± 0.032
1.109IleTyr: 1.109 ± 0.057
0.006IleXaa: 0.006 ± 0.004
Lys
3.379LysAla: 3.379 ± 0.107
0.162LysCys: 0.162 ± 0.02
1.268LysAsp: 1.268 ± 0.056
1.805LysGlu: 1.805 ± 0.064
0.685LysPhe: 0.685 ± 0.043
2.27LysGly: 2.27 ± 0.101
0.669LysHis: 0.669 ± 0.044
1.112LysIle: 1.112 ± 0.055
1.268LysLys: 1.268 ± 0.072
2.892LysLeu: 2.892 ± 0.094
0.729LysMet: 0.729 ± 0.045
0.677LysAsn: 0.677 ± 0.046
1.733LysPro: 1.733 ± 0.072
1.428LysGln: 1.428 ± 0.06
2.751LysArg: 2.751 ± 0.094
1.593LysSer: 1.593 ± 0.071
1.442LysThr: 1.442 ± 0.059
2.19LysVal: 2.19 ± 0.081
0.327LysTrp: 0.327 ± 0.029
0.468LysTyr: 0.468 ± 0.033
0.006LysXaa: 0.006 ± 0.004
Leu
13.779LeuAla: 13.779 ± 0.216
1.158LeuCys: 1.158 ± 0.052
6.309LeuAsp: 6.309 ± 0.128
6.727LeuGlu: 6.727 ± 0.157
3.86LeuPhe: 3.86 ± 0.123
8.664LeuGly: 8.664 ± 0.187
2.388LeuHis: 2.388 ± 0.087
5.384LeuIle: 5.384 ± 0.14
3.39LeuLys: 3.39 ± 0.109
12.472LeuLeu: 12.472 ± 0.291
3.23LeuMet: 3.23 ± 0.107
3.258LeuAsn: 3.258 ± 0.096
6.213LeuPro: 6.213 ± 0.134
3.657LeuGln: 3.657 ± 0.108
6.292LeuArg: 6.292 ± 0.135
7.473LeuSer: 7.473 ± 0.158
6.353LeuThr: 6.353 ± 0.151
7.575LeuVal: 7.575 ± 0.178
1.516LeuTrp: 1.516 ± 0.067
2.363LeuTyr: 2.363 ± 0.085
0.006LeuXaa: 0.006 ± 0.004
Met
3.695MetAla: 3.695 ± 0.105
0.187MetCys: 0.187 ± 0.024
1.235MetAsp: 1.235 ± 0.051
1.337MetGlu: 1.337 ± 0.065
0.638MetPhe: 0.638 ± 0.046
2.022MetGly: 2.022 ± 0.079
0.567MetHis: 0.567 ± 0.041
1.315MetIle: 1.315 ± 0.063
0.924MetLys: 0.924 ± 0.052
3.073MetLeu: 3.073 ± 0.094
0.721MetMet: 0.721 ± 0.043
0.908MetAsn: 0.908 ± 0.057
1.563MetPro: 1.563 ± 0.065
1.26MetGln: 1.26 ± 0.053
1.791MetArg: 1.791 ± 0.081
2.055MetSer: 2.055 ± 0.067
1.942MetThr: 1.942 ± 0.069
1.975MetVal: 1.975 ± 0.076
0.226MetTrp: 0.226 ± 0.026
0.435MetTyr: 0.435 ± 0.039
0.003MetXaa: 0.003 ± 0.002
Asn
3.42AsnAla: 3.42 ± 0.103
0.278AsnCys: 0.278 ± 0.028
1.673AsnAsp: 1.673 ± 0.075
1.629AsnGlu: 1.629 ± 0.071
0.974AsnPhe: 0.974 ± 0.055
2.352AsnGly: 2.352 ± 0.089
0.636AsnHis: 0.636 ± 0.042
1.337AsnIle: 1.337 ± 0.067
0.674AsnLys: 0.674 ± 0.05
2.625AsnLeu: 2.625 ± 0.096
0.663AsnMet: 0.663 ± 0.05
0.817AsnAsn: 0.817 ± 0.044
1.676AsnPro: 1.676 ± 0.069
1.329AsnGln: 1.329 ± 0.052
1.805AsnArg: 1.805 ± 0.062
1.376AsnSer: 1.376 ± 0.063
1.513AsnThr: 1.513 ± 0.074
2.253AsnVal: 2.253 ± 0.085
0.41AsnTrp: 0.41 ± 0.031
0.685AsnTyr: 0.685 ± 0.045
0.006AsnXaa: 0.006 ± 0.004
Pro
4.776ProAla: 4.776 ± 0.122
0.426ProCys: 0.426 ± 0.034
2.82ProAsp: 2.82 ± 0.091
3.624ProGlu: 3.624 ± 0.109
1.832ProPhe: 1.832 ± 0.072
3.728ProGly: 3.728 ± 0.115
1.249ProHis: 1.249 ± 0.059
2.05ProIle: 2.05 ± 0.071
1.332ProLys: 1.332 ± 0.062
5.77ProLeu: 5.77 ± 0.14
1.414ProMet: 1.414 ± 0.053
1.618ProAsn: 1.618 ± 0.081
2.314ProPro: 2.314 ± 0.08
1.945ProGln: 1.945 ± 0.078
2.597ProArg: 2.597 ± 0.088
3.126ProSer: 3.126 ± 0.086
2.589ProThr: 2.589 ± 0.074
3.442ProVal: 3.442 ± 0.101
0.864ProTrp: 0.864 ± 0.053
1.059ProTyr: 1.059 ± 0.05
0.008ProXaa: 0.008 ± 0.005
Gln
5.926GlnAla: 5.926 ± 0.164
0.421GlnCys: 0.421 ± 0.034
1.703GlnAsp: 1.703 ± 0.068
2.416GlnGlu: 2.416 ± 0.072
1.257GlnPhe: 1.257 ± 0.06
3.508GlnGly: 3.508 ± 0.095
1.389GlnHis: 1.389 ± 0.069
1.786GlnIle: 1.786 ± 0.072
1.172GlnLys: 1.172 ± 0.06
4.963GlnLeu: 4.963 ± 0.131
1.046GlnMet: 1.046 ± 0.053
0.902GlnAsn: 0.902 ± 0.053
2.369GlnPro: 2.369 ± 0.074
3.282GlnGln: 3.282 ± 0.11
4.034GlnArg: 4.034 ± 0.115
2.35GlnSer: 2.35 ± 0.086
1.992GlnThr: 1.992 ± 0.07
3.2GlnVal: 3.2 ± 0.115
0.897GlnTrp: 0.897 ± 0.051
0.79GlnTyr: 0.79 ± 0.05
0.0GlnXaa: 0.0 ± 0.0
Arg
5.715ArgAla: 5.715 ± 0.137
0.828ArgCys: 0.828 ± 0.046
3.472ArgAsp: 3.472 ± 0.097
4.157ArgGlu: 4.157 ± 0.126
2.955ArgPhe: 2.955 ± 0.077
4.028ArgGly: 4.028 ± 0.102
2.207ArgHis: 2.207 ± 0.071
3.153ArgIle: 3.153 ± 0.096
1.764ArgLys: 1.764 ± 0.073
8.265ArgLeu: 8.265 ± 0.182
1.951ArgMet: 1.951 ± 0.067
1.533ArgAsn: 1.533 ± 0.063
2.801ArgPro: 2.801 ± 0.088
3.657ArgGln: 3.657 ± 0.108
4.875ArgArg: 4.875 ± 0.139
3.304ArgSer: 3.304 ± 0.104
2.694ArgThr: 2.694 ± 0.086
4.386ArgVal: 4.386 ± 0.103
1.244ArgTrp: 1.244 ± 0.06
2.19ArgTyr: 2.19 ± 0.084
0.008ArgXaa: 0.008 ± 0.004
Ser
6.383SerAla: 6.383 ± 0.131
0.465SerCys: 0.465 ± 0.039
3.208SerAsp: 3.208 ± 0.113
3.222SerGlu: 3.222 ± 0.114
2.124SerPhe: 2.124 ± 0.08
5.362SerGly: 5.362 ± 0.114
1.461SerHis: 1.461 ± 0.071
2.462SerIle: 2.462 ± 0.083
1.444SerLys: 1.444 ± 0.074
6.947SerLeu: 6.947 ± 0.153
1.717SerMet: 1.717 ± 0.07
1.568SerAsn: 1.568 ± 0.074
2.787SerPro: 2.787 ± 0.082
2.63SerGln: 2.63 ± 0.092
3.866SerArg: 3.866 ± 0.101
3.703SerSer: 3.703 ± 0.113
3.255SerThr: 3.255 ± 0.091
4.234SerVal: 4.234 ± 0.124
0.856SerTrp: 0.856 ± 0.043
1.417SerTyr: 1.417 ± 0.066
0.008SerXaa: 0.008 ± 0.005
Thr
5.272ThrAla: 5.272 ± 0.126
0.517ThrCys: 0.517 ± 0.037
2.545ThrAsp: 2.545 ± 0.082
2.482ThrGlu: 2.482 ± 0.091
2.138ThrPhe: 2.138 ± 0.073
4.053ThrGly: 4.053 ± 0.116
1.348ThrHis: 1.348 ± 0.058
2.242ThrIle: 2.242 ± 0.075
1.233ThrLys: 1.233 ± 0.061
7.993ThrLeu: 7.993 ± 0.14
1.241ThrMet: 1.241 ± 0.059
1.227ThrAsn: 1.227 ± 0.058
3.868ThrPro: 3.868 ± 0.104
2.443ThrGln: 2.443 ± 0.085
3.266ThrArg: 3.266 ± 0.102
3.09ThrSer: 3.09 ± 0.101
2.905ThrThr: 2.905 ± 0.109
3.772ThrVal: 3.772 ± 0.124
0.74ThrTrp: 0.74 ± 0.051
1.153ThrTyr: 1.153 ± 0.06
0.006ThrXaa: 0.006 ± 0.004
Val
8.042ValAla: 8.042 ± 0.164
0.801ValCys: 0.801 ± 0.045
3.989ValAsp: 3.989 ± 0.113
4.556ValGlu: 4.556 ± 0.112
2.468ValPhe: 2.468 ± 0.087
5.434ValGly: 5.434 ± 0.139
1.307ValHis: 1.307 ± 0.062
3.866ValIle: 3.866 ± 0.109
2.094ValLys: 2.094 ± 0.089
7.374ValLeu: 7.374 ± 0.178
2.267ValMet: 2.267 ± 0.077
2.396ValAsn: 2.396 ± 0.09
3.216ValPro: 3.216 ± 0.086
2.248ValGln: 2.248 ± 0.075
4.034ValArg: 4.034 ± 0.111
4.655ValSer: 4.655 ± 0.111
4.496ValThr: 4.496 ± 0.11
6.045ValVal: 6.045 ± 0.157
0.974ValTrp: 0.974 ± 0.055
1.499ValTyr: 1.499 ± 0.062
0.008ValXaa: 0.008 ± 0.004
Trp
1.2TrpAla: 1.2 ± 0.062
0.215TrpCys: 0.215 ± 0.025
0.526TrpAsp: 0.526 ± 0.04
0.773TrpGlu: 0.773 ± 0.049
0.608TrpPhe: 0.608 ± 0.043
0.913TrpGly: 0.913 ± 0.058
0.44TrpHis: 0.44 ± 0.039
0.603TrpIle: 0.603 ± 0.039
0.399TrpLys: 0.399 ± 0.03
2.449TrpLeu: 2.449 ± 0.079
0.468TrpMet: 0.468 ± 0.039
0.38TrpAsn: 0.38 ± 0.035
0.773TrpPro: 0.773 ± 0.045
1.213TrpGln: 1.213 ± 0.067
1.233TrpArg: 1.233 ± 0.062
0.781TrpSer: 0.781 ± 0.044
0.649TrpThr: 0.649 ± 0.045
1.205TrpVal: 1.205 ± 0.064
0.305TrpTrp: 0.305 ± 0.034
0.374TrpTyr: 0.374 ± 0.032
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.344TyrAla: 2.344 ± 0.085
0.327TyrCys: 0.327 ± 0.034
1.329TyrAsp: 1.329 ± 0.072
1.29TyrGlu: 1.29 ± 0.063
0.861TyrPhe: 0.861 ± 0.056
2.108TyrGly: 2.108 ± 0.086
0.735TyrHis: 0.735 ± 0.043
0.817TyrIle: 0.817 ± 0.046
0.501TyrLys: 0.501 ± 0.034
2.705TyrLeu: 2.705 ± 0.093
0.55TyrMet: 0.55 ± 0.04
0.528TyrAsn: 0.528 ± 0.039
1.271TyrPro: 1.271 ± 0.065
1.235TyrGln: 1.235 ± 0.062
2.036TyrArg: 2.036 ± 0.08
1.279TyrSer: 1.279 ± 0.054
1.227TyrThr: 1.227 ± 0.062
1.626TyrVal: 1.626 ± 0.059
0.44TyrTrp: 0.44 ± 0.035
0.737TyrTyr: 0.737 ± 0.061
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.008XaaAla: 0.008 ± 0.004
0.003XaaCys: 0.003 ± 0.002
0.014XaaAsp: 0.014 ± 0.005
0.003XaaGlu: 0.003 ± 0.003
0.006XaaPhe: 0.006 ± 0.004
0.014XaaGly: 0.014 ± 0.007
0.0XaaHis: 0.0 ± 0.0
0.008XaaIle: 0.008 ± 0.005
0.003XaaLys: 0.003 ± 0.003
0.014XaaLeu: 0.014 ± 0.006
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.008XaaPro: 0.008 ± 0.004
0.0XaaGln: 0.0 ± 0.0
0.008XaaArg: 0.008 ± 0.005
0.014XaaSer: 0.014 ± 0.006
0.0XaaThr: 0.0 ± 0.0
0.011XaaVal: 0.011 ± 0.005
0.0XaaTrp: 0.0 ± 0.0
0.003XaaTyr: 0.003 ± 0.003
0.006XaaXaa: 0.006 ± 0.004
Statistics based on 2023 proteins (363457 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski