Oligella urethralis DNF00040
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2118 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A096BDZ9|A0A096BDZ9_9BURK Lysine decarboxylase OS=Oligella urethralis DNF00040 OX=1401065 GN=HMPREF2130_03485 PE=3 SV=1
MM1 pKa = 7.46 QDD3 pKa = 3.34 YY4 pKa = 11.1 LSTTFHH10 pKa = 6.3 RR11 pKa = 11.84 HH12 pKa = 3.92 INAHH16 pKa = 5.86 QGDD19 pKa = 4.07 TLEE22 pKa = 5.79 DD23 pKa = 2.89 IGLEE27 pKa = 3.75 LRR29 pKa = 11.84 ANVEE33 pKa = 4.0 GSQLFLCFVIAPTDD47 pKa = 3.77 YY48 pKa = 11.16 EE49 pKa = 4.8 LLPQILPEE57 pKa = 4.09 EE58 pKa = 4.15 RR59 pKa = 11.84 FEE61 pKa = 4.1 MGSVHH66 pKa = 6.07 VAEE69 pKa = 4.36 LHH71 pKa = 6.04 EE72 pKa = 5.14 LNDD75 pKa = 4.11 PEE77 pKa = 5.77 DD78 pKa = 4.71 EE79 pKa = 4.31 ISSILDD85 pKa = 3.33 NANDD89 pKa = 3.8 GDD91 pKa = 4.17 TLVFFCASGEE101 pKa = 4.03 ILAAALAALAYY112 pKa = 10.12 NEE114 pKa = 4.4 EE115 pKa = 4.41 SSAFF119 pKa = 3.61
Molecular weight: 13.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 3.973
IPC_protein 3.91
Toseland 3.732
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.757
Rodwell 3.745
Grimsley 3.643
Solomon 3.859
Lehninger 3.808
Nozaki 3.986
DTASelect 4.126
Thurlkill 3.77
EMBOSS 3.783
Sillero 4.024
Patrickios 0.947
IPC_peptide 3.859
IPC2_peptide 4.012
IPC2.peptide.svr19 3.893
Protein with the highest isoelectric point:
>tr|A0A095Z701|A0A095Z701_9BURK Cell shape-determining protein MreB OS=Oligella urethralis DNF00040 OX=1401065 GN=mreB PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.0 RR14 pKa = 11.84 THH16 pKa = 5.76 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 9.2 TKK25 pKa = 10.25 GGRR28 pKa = 11.84 AVINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.58 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2118
0
2118
687764
37
3338
324.7
36.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.979 ± 0.062
0.869 ± 0.016
5.26 ± 0.042
6.341 ± 0.067
4.031 ± 0.041
6.91 ± 0.057
2.258 ± 0.026
6.194 ± 0.042
4.722 ± 0.051
10.709 ± 0.088
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.534 ± 0.025
3.693 ± 0.041
4.207 ± 0.036
4.634 ± 0.057
5.447 ± 0.049
6.175 ± 0.045
4.889 ± 0.042
6.933 ± 0.044
1.227 ± 0.021
2.989 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here