Azospirillum thermophilum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Azospirillaceae; Azospirillum

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5547 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S2CY96|A0A2S2CY96_9PROT Aryl-sulfate sulfotransferase OS=Azospirillum thermophilum OX=2202148 GN=DEW08_25700 PE=4 SV=1
MM1 pKa = 7.68LGTPGNDD8 pKa = 3.1TLYY11 pKa = 9.52GTSGNDD17 pKa = 3.35LLEE20 pKa = 4.48GLTGNDD26 pKa = 3.3ALYY29 pKa = 10.94GFAGNDD35 pKa = 3.5TLIGGDD41 pKa = 4.12GDD43 pKa = 5.48DD44 pKa = 4.38ILDD47 pKa = 4.36GGSGDD52 pKa = 3.93DD53 pKa = 4.3SMVGGAGNDD62 pKa = 3.51VYY64 pKa = 10.97IVDD67 pKa = 4.36SNSDD71 pKa = 4.03RR72 pKa = 11.84ITEE75 pKa = 4.06LAGEE79 pKa = 4.92GIDD82 pKa = 3.87EE83 pKa = 4.38VRR85 pKa = 11.84TSLSSYY91 pKa = 10.89SLASTPDD98 pKa = 3.56VEE100 pKa = 4.35NLTYY104 pKa = 10.14TGPGAFRR111 pKa = 11.84GTGNALANHH120 pKa = 6.86IKK122 pKa = 10.71GKK124 pKa = 10.34DD125 pKa = 3.62GNDD128 pKa = 3.38TLEE131 pKa = 4.29GAKK134 pKa = 10.84GEE136 pKa = 4.19DD137 pKa = 3.6TLEE140 pKa = 4.37GGNGNDD146 pKa = 2.7IYY148 pKa = 10.45MVYY151 pKa = 10.8GEE153 pKa = 4.71GVTIVEE159 pKa = 4.18AAGAGIDD166 pKa = 3.74EE167 pKa = 4.96VYY169 pKa = 7.77TTLSTYY175 pKa = 7.38TLPDD179 pKa = 3.33GVEE182 pKa = 3.85KK183 pKa = 10.02LTYY186 pKa = 9.11TGNGPFTGIGNALNNRR202 pKa = 11.84LWGPEE207 pKa = 3.5GSTLAGGGGDD217 pKa = 4.06DD218 pKa = 5.62LYY220 pKa = 10.79IISDD224 pKa = 3.46KK225 pKa = 10.75NIRR228 pKa = 11.84IIEE231 pKa = 4.13KK232 pKa = 9.65AGEE235 pKa = 4.6GIDD238 pKa = 4.25EE239 pKa = 4.44IRR241 pKa = 11.84TSLTEE246 pKa = 3.54YY247 pKa = 10.18TLADD251 pKa = 3.55KK252 pKa = 11.42EE253 pKa = 4.3EE254 pKa = 4.3VEE256 pKa = 4.18NLTYY260 pKa = 10.85AGTDD264 pKa = 2.45IVTFIGTGNLKK275 pKa = 10.86NNVITSGSGNDD286 pKa = 3.59TLDD289 pKa = 3.48GGGGIDD295 pKa = 3.93TLSGGAGDD303 pKa = 3.98DD304 pKa = 3.53VYY306 pKa = 11.7YY307 pKa = 10.84VDD309 pKa = 5.82DD310 pKa = 3.9VADD313 pKa = 3.65VVFEE317 pKa = 4.02QVNSGFDD324 pKa = 3.28EE325 pKa = 4.14VRR327 pKa = 11.84ARR329 pKa = 11.84VANYY333 pKa = 10.12SLSNNLEE340 pKa = 3.66QLTFIGSGAFNGTGNSLANVITGGDD365 pKa = 3.82GNDD368 pKa = 3.52TLTGGSGNDD377 pKa = 3.46TLLGAGGSDD386 pKa = 3.36NLIGGDD392 pKa = 3.95GNDD395 pKa = 3.46VLLGGDD401 pKa = 4.3GNDD404 pKa = 3.72MLDD407 pKa = 3.59GGTGNDD413 pKa = 3.89SLDD416 pKa = 3.62GGTGGDD422 pKa = 4.07SFDD425 pKa = 4.46GGAGDD430 pKa = 5.74DD431 pKa = 4.0IYY433 pKa = 11.89VVDD436 pKa = 4.24NVNDD440 pKa = 3.4IVNEE444 pKa = 4.06VGGGGNDD451 pKa = 3.53TVRR454 pKa = 11.84TSLDD458 pKa = 3.66SYY460 pKa = 11.69KK461 pKa = 9.84LTPWVEE467 pKa = 3.87TLIYY471 pKa = 9.84TGGSAFTGTGNDD483 pKa = 3.19LANRR487 pKa = 11.84LEE489 pKa = 4.29GRR491 pKa = 11.84DD492 pKa = 4.0GNDD495 pKa = 3.15TLDD498 pKa = 3.56GRR500 pKa = 11.84EE501 pKa = 4.17GSDD504 pKa = 3.3TLVGGAGDD512 pKa = 3.8DD513 pKa = 3.92VYY515 pKa = 11.06IVDD518 pKa = 4.7SSADD522 pKa = 3.8TIIEE526 pKa = 4.13MANGGNDD533 pKa = 3.44TVRR536 pKa = 11.84TSLSSYY542 pKa = 10.05TLGEE546 pKa = 4.06TSNLEE551 pKa = 3.66NLAFIGSGAFTGTGNSLANAITGGVLSDD579 pKa = 3.91TLSGGAGNDD588 pKa = 3.7TLDD591 pKa = 3.93GGAGNDD597 pKa = 3.88SLIGGAGDD605 pKa = 3.65DD606 pKa = 4.02VYY608 pKa = 11.06IVDD611 pKa = 4.16SSADD615 pKa = 3.24IVVEE619 pKa = 4.01IANGGIDD626 pKa = 3.84TVCTSLGSYY635 pKa = 8.8TLGEE639 pKa = 4.18TSNLEE644 pKa = 3.66NLAFIGSGAFTGTGNSLANAIAGGSGNDD672 pKa = 3.79SLSGGAGNDD681 pKa = 3.46TLTGGLGNDD690 pKa = 4.03TLDD693 pKa = 3.86GGAGNDD699 pKa = 3.88SLIGGAGDD707 pKa = 3.65DD708 pKa = 4.02VYY710 pKa = 11.06IVDD713 pKa = 4.16SSADD717 pKa = 3.3IVVEE721 pKa = 4.06TANGGIDD728 pKa = 3.49TVRR731 pKa = 11.84TSLGSYY737 pKa = 8.91TLGEE741 pKa = 4.18TSNLEE746 pKa = 3.66NLAFIGSGAFTGTGNSLANAIAGGSGNDD774 pKa = 3.69NLSGGAGNDD783 pKa = 3.7TLDD786 pKa = 3.93GGAGNDD792 pKa = 3.7SLIGGVGDD800 pKa = 4.04DD801 pKa = 3.99VYY803 pKa = 11.07IVDD806 pKa = 4.16SSADD810 pKa = 3.24IVVEE814 pKa = 4.01IANGGIDD821 pKa = 3.57TVRR824 pKa = 11.84TSLSGYY830 pKa = 7.68TLGEE834 pKa = 3.97TSNLEE839 pKa = 3.58NLAYY843 pKa = 10.13FGSGAFTGTGNSLANAIAGGSGNDD867 pKa = 3.67SLSGGDD873 pKa = 4.41GNDD876 pKa = 3.34TLTGGLGNDD885 pKa = 3.86TLIGGAGEE893 pKa = 4.12DD894 pKa = 3.59TAVFAAGTIVWRR906 pKa = 11.84DD907 pKa = 2.66IDD909 pKa = 3.62GTEE912 pKa = 4.06RR913 pKa = 11.84AFGPDD918 pKa = 3.47GEE920 pKa = 5.09DD921 pKa = 3.04VLSGIEE927 pKa = 3.93RR928 pKa = 11.84VRR930 pKa = 11.84IGAGAAVPIEE940 pKa = 4.05QSRR943 pKa = 11.84FNPWAYY949 pKa = 10.13LASYY953 pKa = 10.56SDD955 pKa = 3.5LRR957 pKa = 11.84AAFGSDD963 pKa = 2.52GAAAVRR969 pKa = 11.84HH970 pKa = 4.65YY971 pKa = 11.09LEE973 pKa = 4.19YY974 pKa = 11.14GRR976 pKa = 11.84DD977 pKa = 3.38EE978 pKa = 4.08GRR980 pKa = 11.84GIIFDD985 pKa = 4.05TLSYY989 pKa = 8.46TASYY993 pKa = 10.52SDD995 pKa = 4.89LIGAFGTNVDD1005 pKa = 3.35AAARR1009 pKa = 11.84HH1010 pKa = 5.03YY1011 pKa = 10.61LEE1013 pKa = 5.13HH1014 pKa = 6.53GRR1016 pKa = 11.84SEE1018 pKa = 3.92GRR1020 pKa = 11.84GIVFDD1025 pKa = 3.13AHH1027 pKa = 7.06RR1028 pKa = 11.84YY1029 pKa = 7.16LDD1031 pKa = 3.72KK1032 pKa = 11.53YY1033 pKa = 11.32ADD1035 pKa = 3.58LKK1037 pKa = 11.37AAFGDD1042 pKa = 3.81DD1043 pKa = 3.09TVAATRR1049 pKa = 11.84QFILEE1054 pKa = 4.75GYY1056 pKa = 8.42AQGRR1060 pKa = 11.84NLEE1063 pKa = 4.1AAVVNGTAAAEE1074 pKa = 4.08ILQGTGIEE1082 pKa = 4.49DD1083 pKa = 4.59IITGGLGNDD1092 pKa = 3.81TLIGGAGEE1100 pKa = 4.12DD1101 pKa = 3.59TAVFAAGTIVWRR1113 pKa = 11.84DD1114 pKa = 2.66IDD1116 pKa = 3.62GTEE1119 pKa = 4.06RR1120 pKa = 11.84AFGPDD1125 pKa = 3.47GEE1127 pKa = 5.09DD1128 pKa = 3.04VLSGIEE1134 pKa = 3.93RR1135 pKa = 11.84VRR1137 pKa = 11.84IGAGPAVPIEE1147 pKa = 4.07QSRR1150 pKa = 11.84FNPWAYY1156 pKa = 10.13LASYY1160 pKa = 10.56SDD1162 pKa = 3.5LRR1164 pKa = 11.84AAFGSDD1170 pKa = 2.75GAAAVHH1176 pKa = 6.79HH1177 pKa = 5.43YY1178 pKa = 10.79LEE1180 pKa = 4.4YY1181 pKa = 11.17GRR1183 pKa = 11.84DD1184 pKa = 3.38EE1185 pKa = 4.08GRR1187 pKa = 11.84GIIFDD1192 pKa = 4.04ALSYY1196 pKa = 8.27TASYY1200 pKa = 10.62SDD1202 pKa = 4.89LIGAFGTDD1210 pKa = 3.05AEE1212 pKa = 4.62AAAHH1216 pKa = 6.6HH1217 pKa = 5.84YY1218 pKa = 10.42LSYY1221 pKa = 10.98GLRR1224 pKa = 11.84EE1225 pKa = 4.0GRR1227 pKa = 11.84GITFDD1232 pKa = 3.27AQHH1235 pKa = 6.15YY1236 pKa = 8.59LDD1238 pKa = 5.29RR1239 pKa = 11.84YY1240 pKa = 10.26ADD1242 pKa = 3.79LKK1244 pKa = 11.3AAFGNDD1250 pKa = 2.56TAAATRR1256 pKa = 11.84HH1257 pKa = 5.15YY1258 pKa = 9.7IQYY1261 pKa = 10.64GFFEE1265 pKa = 4.53GRR1267 pKa = 11.84LTT1269 pKa = 3.89

Molecular weight: 128.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S2CVW3|A0A2S2CVW3_9PROT Uncharacterized protein OS=Azospirillum thermophilum OX=2202148 GN=DEW08_21165 PE=4 SV=1
MM1 pKa = 7.42RR2 pKa = 11.84QPRR5 pKa = 11.84TRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84PAPARR14 pKa = 11.84PWGKK18 pKa = 9.55RR19 pKa = 11.84SRR21 pKa = 11.84RR22 pKa = 11.84PSPRR26 pKa = 11.84SPAPRR31 pKa = 11.84PSRR34 pKa = 11.84SPCAPRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84SLRR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84SSRR50 pKa = 11.84PRR52 pKa = 11.84SPPRR56 pKa = 11.84PLSRR60 pKa = 11.84LRR62 pKa = 11.84PRR64 pKa = 11.84HH65 pKa = 5.74SRR67 pKa = 11.84RR68 pKa = 11.84PWQSRR73 pKa = 3.07

Molecular weight: 8.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5547

0

5547

1799258

26

6016

324.4

34.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.401 ± 0.048

0.865 ± 0.012

5.66 ± 0.03

5.587 ± 0.033

3.28 ± 0.021

9.107 ± 0.035

1.99 ± 0.016

4.284 ± 0.024

2.57 ± 0.031

10.748 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.315 ± 0.016

2.163 ± 0.021

5.684 ± 0.036

2.969 ± 0.024

8.085 ± 0.035

4.851 ± 0.026

5.293 ± 0.037

7.845 ± 0.029

1.291 ± 0.012

2.013 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski