Amino acid dipepetide frequency for Azospirillum thermophilum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.397AlaAla: 21.397 ± 0.177	1.27AlaCys: 1.27 ± 0.029	7.426AlaAsp: 7.426 ± 0.07	8.311AlaGlu: 8.311 ± 0.083	4.056AlaPhe: 4.056 ± 0.053	13.361AlaGly: 13.361 ± 0.128	2.148AlaHis: 2.148 ± 0.039	5.25AlaIle: 5.25 ± 0.062	3.211AlaLys: 3.211 ± 0.048	14.871AlaLeu: 14.871 ± 0.109	3.556AlaMet: 3.556 ± 0.048	2.557AlaAsn: 2.557 ± 0.044	6.242AlaPro: 6.242 ± 0.081	3.737AlaGln: 3.737 ± 0.063	9.845AlaArg: 9.845 ± 0.099	5.622AlaSer: 5.622 ± 0.073	6.22AlaThr: 6.22 ± 0.075	11.065AlaVal: 11.065 ± 0.094	1.506AlaTrp: 1.506 ± 0.032	2.355AlaTyr: 2.355 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.997CysAla: 0.997 ± 0.026	0.128CysCys: 0.128 ± 0.008	0.527CysAsp: 0.527 ± 0.019	0.387CysGlu: 0.387 ± 0.014	0.296CysPhe: 0.296 ± 0.012	1.018CysGly: 1.018 ± 0.026	0.251CysHis: 0.251 ± 0.014	0.357CysIle: 0.357 ± 0.016	0.153CysLys: 0.153 ± 0.01	0.836CysLeu: 0.836 ± 0.021	0.166CysMet: 0.166 ± 0.009	0.191CysAsn: 0.191 ± 0.01	0.525CysPro: 0.525 ± 0.021	0.208CysGln: 0.208 ± 0.011	0.835CysArg: 0.835 ± 0.023	0.442CysSer: 0.442 ± 0.017	0.393CysThr: 0.393 ± 0.016	0.619CysVal: 0.619 ± 0.019	0.124CysTrp: 0.124 ± 0.01	0.198CysTyr: 0.198 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	6.826AspAla: 6.826 ± 0.066	0.459AspCys: 0.459 ± 0.016	2.863AspAsp: 2.863 ± 0.059	3.27AspGlu: 3.27 ± 0.047	1.751AspPhe: 1.751 ± 0.031	6.011AspGly: 6.011 ± 0.083	1.332AspHis: 1.332 ± 0.031	2.415AspIle: 2.415 ± 0.035	1.275AspLys: 1.275 ± 0.033	6.454AspLeu: 6.454 ± 0.07	1.131AspMet: 1.131 ± 0.023	1.037AspAsn: 1.037 ± 0.026	3.945AspPro: 3.945 ± 0.048	1.602AspGln: 1.602 ± 0.032	5.439AspArg: 5.439 ± 0.064	2.274AspSer: 2.274 ± 0.042	2.491AspThr: 2.491 ± 0.054	3.802AspVal: 3.802 ± 0.05	0.942AspTrp: 0.942 ± 0.027	1.282AspTyr: 1.282 ± 0.027	0.0AspXaa: 0.0 ± 0.0
Glu	8.112GluAla: 8.112 ± 0.081	0.307GluCys: 0.307 ± 0.012	2.73GluAsp: 2.73 ± 0.041	3.745GluGlu: 3.745 ± 0.058	1.488GluPhe: 1.488 ± 0.029	4.255GluGly: 4.255 ± 0.049	1.075GluHis: 1.075 ± 0.025	2.78GluIle: 2.78 ± 0.036	1.685GluLys: 1.685 ± 0.034	5.464GluLeu: 5.464 ± 0.06	1.384GluMet: 1.384 ± 0.028	1.094GluAsn: 1.094 ± 0.025	2.938GluPro: 2.938 ± 0.047	2.131GluGln: 2.131 ± 0.043	5.625GluArg: 5.625 ± 0.063	2.136GluSer: 2.136 ± 0.03	3.21GluThr: 3.21 ± 0.04	4.293GluVal: 4.293 ± 0.058	0.606GluTrp: 0.606 ± 0.019	0.815GluTyr: 0.815 ± 0.022	0.0GluXaa: 0.0 ± 0.0
Phe	4.18PheAla: 4.18 ± 0.053	0.362PheCys: 0.362 ± 0.015	2.327PheAsp: 2.327 ± 0.035	1.723PheGlu: 1.723 ± 0.033	1.15PhePhe: 1.15 ± 0.028	3.261PheGly: 3.261 ± 0.044	0.716PheHis: 0.716 ± 0.022	1.272PheIle: 1.272 ± 0.031	0.78PheLys: 0.78 ± 0.023	3.37PheLeu: 3.37 ± 0.054	0.614PheMet: 0.614 ± 0.02	0.82PheAsn: 0.82 ± 0.02	1.548PhePro: 1.548 ± 0.035	0.935PheGln: 0.935 ± 0.024	2.355PheArg: 2.355 ± 0.038	1.717PheSer: 1.717 ± 0.033	1.881PheThr: 1.881 ± 0.031	2.522PheVal: 2.522 ± 0.04	0.454PheTrp: 0.454 ± 0.017	0.808PheTyr: 0.808 ± 0.022	0.0PheXaa: 0.0 ± 0.0
Gly	10.03GlyAla: 10.03 ± 0.11	0.988GlyCys: 0.988 ± 0.029	4.721GlyAsp: 4.721 ± 0.071	4.75GlyGlu: 4.75 ± 0.053	3.476GlyPhe: 3.476 ± 0.045	8.455GlyGly: 8.455 ± 0.138	1.942GlyHis: 1.942 ± 0.037	4.268GlyIle: 4.268 ± 0.051	2.689GlyLys: 2.689 ± 0.04	9.925GlyLeu: 9.925 ± 0.083	2.448GlyMet: 2.448 ± 0.038	2.017GlyAsn: 2.017 ± 0.062	4.173GlyPro: 4.173 ± 0.053	2.782GlyGln: 2.782 ± 0.043	8.046GlyArg: 8.046 ± 0.079	4.716GlySer: 4.716 ± 0.082	5.302GlyThr: 5.302 ± 0.091	6.571GlyVal: 6.571 ± 0.06	1.506GlyTrp: 1.506 ± 0.033	2.264GlyTyr: 2.264 ± 0.038	0.0GlyXaa: 0.0 ± 0.0
His	2.378HisAla: 2.378 ± 0.039	0.223HisCys: 0.223 ± 0.014	1.183HisAsp: 1.183 ± 0.029	0.964HisGlu: 0.964 ± 0.022	0.695HisPhe: 0.695 ± 0.021	2.044HisGly: 2.044 ± 0.035	0.588HisHis: 0.588 ± 0.021	0.773HisIle: 0.773 ± 0.022	0.442HisLys: 0.442 ± 0.016	2.137HisLeu: 2.137 ± 0.035	0.442HisMet: 0.442 ± 0.016	0.389HisAsn: 0.389 ± 0.015	1.462HisPro: 1.462 ± 0.031	0.551HisGln: 0.551 ± 0.019	1.772HisArg: 1.772 ± 0.035	0.853HisSer: 0.853 ± 0.026	0.809HisThr: 0.809 ± 0.021	1.395HisVal: 1.395 ± 0.031	0.323HisTrp: 0.323 ± 0.013	0.479HisTyr: 0.479 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	6.295IleAla: 6.295 ± 0.067	0.345IleCys: 0.345 ± 0.013	3.081IleAsp: 3.081 ± 0.049	2.686IleGlu: 2.686 ± 0.045	1.165IlePhe: 1.165 ± 0.025	4.551IleGly: 4.551 ± 0.058	0.855IleHis: 0.855 ± 0.021	1.461IleIle: 1.461 ± 0.031	0.965IleLys: 0.965 ± 0.023	4.231IleLeu: 4.231 ± 0.058	0.738IleMet: 0.738 ± 0.022	1.127IleAsn: 1.127 ± 0.029	2.143IlePro: 2.143 ± 0.038	1.09IleGln: 1.09 ± 0.026	3.222IleArg: 3.222 ± 0.042	1.947IleSer: 1.947 ± 0.038	2.195IleThr: 2.195 ± 0.045	3.524IleVal: 3.524 ± 0.046	0.403IleTrp: 0.403 ± 0.016	0.813IleTyr: 0.813 ± 0.022	0.0IleXaa: 0.0 ± 0.0
Lys	3.607LysAla: 3.607 ± 0.052	0.121LysCys: 0.121 ± 0.008	1.434LysAsp: 1.434 ± 0.033	1.406LysGlu: 1.406 ± 0.032	0.608LysPhe: 0.608 ± 0.023	2.287LysGly: 2.287 ± 0.043	0.426LysHis: 0.426 ± 0.016	1.092LysIle: 1.092 ± 0.03	0.842LysLys: 0.842 ± 0.027	2.561LysLeu: 2.561 ± 0.047	0.584LysMet: 0.584 ± 0.021	0.545LysAsn: 0.545 ± 0.017	1.837LysPro: 1.837 ± 0.04	0.776LysGln: 0.776 ± 0.023	2.016LysArg: 2.016 ± 0.039	1.297LysSer: 1.297 ± 0.03	1.492LysThr: 1.492 ± 0.034	2.063LysVal: 2.063 ± 0.037	0.246LysTrp: 0.246 ± 0.012	0.458LysTyr: 0.458 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	15.261LeuAla: 15.261 ± 0.117	0.971LeuCys: 0.971 ± 0.025	6.414LeuAsp: 6.414 ± 0.065	5.531LeuGlu: 5.531 ± 0.057	3.787LeuPhe: 3.787 ± 0.054	8.677LeuGly: 8.677 ± 0.077	2.098LeuHis: 2.098 ± 0.04	4.322LeuIle: 4.322 ± 0.06	2.967LeuLys: 2.967 ± 0.047	11.365LeuLeu: 11.365 ± 0.112	2.321LeuMet: 2.321 ± 0.041	2.299LeuAsn: 2.299 ± 0.033	6.38LeuPro: 6.38 ± 0.068	2.779LeuGln: 2.779 ± 0.037	8.334LeuArg: 8.334 ± 0.097	6.375LeuSer: 6.375 ± 0.069	6.091LeuThr: 6.091 ± 0.068	8.222LeuVal: 8.222 ± 0.079	1.247LeuTrp: 1.247 ± 0.03	2.045LeuTyr: 2.045 ± 0.033	0.0LeuXaa: 0.0 ± 0.0
Met	3.351MetAla: 3.351 ± 0.045	0.129MetCys: 0.129 ± 0.008	1.144MetAsp: 1.144 ± 0.025	1.196MetGlu: 1.196 ± 0.027	0.562MetPhe: 0.562 ± 0.018	1.709MetGly: 1.709 ± 0.03	0.377MetHis: 0.377 ± 0.015	1.044MetIle: 1.044 ± 0.026	0.743MetLys: 0.743 ± 0.022	2.425MetLeu: 2.425 ± 0.04	0.61MetMet: 0.61 ± 0.02	0.588MetAsn: 0.588 ± 0.019	1.471MetPro: 1.471 ± 0.034	0.685MetGln: 0.685 ± 0.018	1.669MetArg: 1.669 ± 0.031	1.336MetSer: 1.336 ± 0.028	1.817MetThr: 1.817 ± 0.029	1.832MetVal: 1.832 ± 0.037	0.191MetTrp: 0.191 ± 0.01	0.27MetTyr: 0.27 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.703AsnAla: 2.703 ± 0.046	0.191AsnCys: 0.191 ± 0.01	1.236AsnAsp: 1.236 ± 0.051	0.973AsnGlu: 0.973 ± 0.021	0.645AsnPhe: 0.645 ± 0.021	2.145AsnGly: 2.145 ± 0.044	0.46AsnHis: 0.46 ± 0.016	0.961AsnIle: 0.961 ± 0.026	0.515AsnLys: 0.515 ± 0.019	2.373AsnLeu: 2.373 ± 0.047	0.422AsnMet: 0.422 ± 0.015	0.543AsnAsn: 0.543 ± 0.02	1.658AsnPro: 1.658 ± 0.034	0.641AsnGln: 0.641 ± 0.019	1.78AsnArg: 1.78 ± 0.032	0.999AsnSer: 0.999 ± 0.023	1.041AsnThr: 1.041 ± 0.027	1.56AsnVal: 1.56 ± 0.03	0.306AsnTrp: 0.306 ± 0.015	0.478AsnTyr: 0.478 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	8.438ProAla: 8.438 ± 0.1	0.411ProCys: 0.411 ± 0.017	4.23ProAsp: 4.23 ± 0.057	3.724ProGlu: 3.724 ± 0.055	1.881ProPhe: 1.881 ± 0.036	5.344ProGly: 5.344 ± 0.065	1.039ProHis: 1.039 ± 0.024	1.859ProIle: 1.859 ± 0.033	1.372ProLys: 1.372 ± 0.028	5.432ProLeu: 5.432 ± 0.054	1.168ProMet: 1.168 ± 0.026	1.137ProAsn: 1.137 ± 0.025	3.692ProPro: 3.692 ± 0.077	1.639ProGln: 1.639 ± 0.03	3.413ProArg: 3.413 ± 0.052	2.631ProSer: 2.631 ± 0.047	2.501ProThr: 2.501 ± 0.046	5.068ProVal: 5.068 ± 0.057	0.756ProTrp: 0.756 ± 0.02	1.105ProTyr: 1.105 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	4.376GlnAla: 4.376 ± 0.056	0.169GlnCys: 0.169 ± 0.011	1.446GlnAsp: 1.446 ± 0.029	1.582GlnGlu: 1.582 ± 0.031	0.881GlnPhe: 0.881 ± 0.022	2.481GlnGly: 2.481 ± 0.04	0.562GlnHis: 0.562 ± 0.02	1.459GlnIle: 1.459 ± 0.033	0.82GlnLys: 0.82 ± 0.024	2.567GlnLeu: 2.567 ± 0.05	0.735GlnMet: 0.735 ± 0.021	0.608GlnAsn: 0.608 ± 0.021	1.937GlnPro: 1.937 ± 0.037	1.236GlnGln: 1.236 ± 0.038	2.445GlnArg: 2.445 ± 0.038	1.517GlnSer: 1.517 ± 0.031	1.724GlnThr: 1.724 ± 0.04	2.299GlnVal: 2.299 ± 0.04	0.327GlnTrp: 0.327 ± 0.014	0.519GlnTyr: 0.519 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	8.95ArgAla: 8.95 ± 0.082	0.715ArgCys: 0.715 ± 0.021	4.456ArgAsp: 4.456 ± 0.056	4.311ArgGlu: 4.311 ± 0.056	3.142ArgPhe: 3.142 ± 0.046	5.553ArgGly: 5.553 ± 0.055	2.005ArgHis: 2.005 ± 0.039	4.079ArgIle: 4.079 ± 0.048	2.036ArgLys: 2.036 ± 0.037	9.677ArgLeu: 9.677 ± 0.099	2.1ArgMet: 2.1 ± 0.035	1.8ArgAsn: 1.8 ± 0.035	4.702ArgPro: 4.702 ± 0.059	2.814ArgGln: 2.814 ± 0.045	7.879ArgArg: 7.879 ± 0.098	4.142ArgSer: 4.142 ± 0.052	3.983ArgThr: 3.983 ± 0.044	5.59ArgVal: 5.59 ± 0.06	1.163ArgTrp: 1.163 ± 0.028	1.751ArgTyr: 1.751 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	5.866SerAla: 5.866 ± 0.065	0.437SerCys: 0.437 ± 0.015	2.508SerAsp: 2.508 ± 0.042	2.106SerGlu: 2.106 ± 0.038	2.002SerPhe: 2.002 ± 0.04	5.279SerGly: 5.279 ± 0.071	0.971SerHis: 0.971 ± 0.025	2.205SerIle: 2.205 ± 0.041	1.115SerLys: 1.115 ± 0.031	5.322SerLeu: 5.322 ± 0.06	1.099SerMet: 1.099 ± 0.026	1.075SerAsn: 1.075 ± 0.029	2.839SerPro: 2.839 ± 0.044	1.396SerGln: 1.396 ± 0.027	3.652SerArg: 3.652 ± 0.049	2.512SerSer: 2.512 ± 0.045	2.389SerThr: 2.389 ± 0.041	3.831SerVal: 3.831 ± 0.057	0.728SerTrp: 0.728 ± 0.021	1.173SerTyr: 1.173 ± 0.03	0.0SerXaa: 0.0 ± 0.0
Thr	7.095ThrAla: 7.095 ± 0.081	0.39ThrCys: 0.39 ± 0.016	2.84ThrAsp: 2.84 ± 0.046	2.514ThrGlu: 2.514 ± 0.04	1.525ThrPhe: 1.525 ± 0.03	5.649ThrGly: 5.649 ± 0.078	0.883ThrHis: 0.883 ± 0.024	2.551ThrIle: 2.551 ± 0.047	1.109ThrLys: 1.109 ± 0.029	6.227ThrLeu: 6.227 ± 0.08	1.135ThrMet: 1.135 ± 0.025	1.112ThrAsn: 1.112 ± 0.03	3.283ThrPro: 3.283 ± 0.051	1.333ThrGln: 1.333 ± 0.035	3.493ThrArg: 3.493 ± 0.047	2.317ThrSer: 2.317 ± 0.043	2.626ThrThr: 2.626 ± 0.053	5.273ThrVal: 5.273 ± 0.075	0.548ThrTrp: 0.548 ± 0.016	1.025ThrTyr: 1.025 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	10.548ValAla: 10.548 ± 0.094	0.69ValCys: 0.69 ± 0.018	4.072ValAsp: 4.072 ± 0.049	5.046ValGlu: 5.046 ± 0.054	2.522ValPhe: 2.522 ± 0.038	6.051ValGly: 6.051 ± 0.064	1.431ValHis: 1.431 ± 0.031	3.339ValIle: 3.339 ± 0.055	2.141ValLys: 2.141 ± 0.042	8.46ValLeu: 8.46 ± 0.078	1.789ValMet: 1.789 ± 0.035	1.866ValAsn: 1.866 ± 0.042	4.369ValPro: 4.369 ± 0.052	2.205ValGln: 2.205 ± 0.036	5.917ValArg: 5.917 ± 0.06	3.952ValSer: 3.952 ± 0.054	4.994ValThr: 4.994 ± 0.088	6.592ValVal: 6.592 ± 0.07	0.979ValTrp: 0.979 ± 0.025	1.488ValTyr: 1.488 ± 0.031	0.0ValXaa: 0.0 ± 0.0
Trp	1.271TrpAla: 1.271 ± 0.028	0.136TrpCys: 0.136 ± 0.009	0.629TrpAsp: 0.629 ± 0.022	0.57TrpGlu: 0.57 ± 0.019	0.459TrpPhe: 0.459 ± 0.017	0.871TrpGly: 0.871 ± 0.023	0.304TrpHis: 0.304 ± 0.014	0.617TrpIle: 0.617 ± 0.021	0.353TrpLys: 0.353 ± 0.014	1.671TrpLeu: 1.671 ± 0.039	0.336TrpMet: 0.336 ± 0.013	0.332TrpAsn: 0.332 ± 0.014	0.689TrpPro: 0.689 ± 0.019	0.5TrpGln: 0.5 ± 0.014	1.262TrpArg: 1.262 ± 0.031	0.738TrpSer: 0.738 ± 0.021	0.791TrpThr: 0.791 ± 0.022	0.858TrpVal: 0.858 ± 0.023	0.219TrpTrp: 0.219 ± 0.013	0.305TrpTyr: 0.305 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.326TyrAla: 2.326 ± 0.04	0.21TyrCys: 0.21 ± 0.01	1.335TyrAsp: 1.335 ± 0.031	1.08TyrGlu: 1.08 ± 0.027	0.703TyrPhe: 0.703 ± 0.021	2.065TyrGly: 2.065 ± 0.034	0.44TyrHis: 0.44 ± 0.016	0.735TyrIle: 0.735 ± 0.02	0.486TyrLys: 0.486 ± 0.017	2.118TyrLeu: 2.118 ± 0.035	0.37TyrMet: 0.37 ± 0.015	0.492TyrAsn: 0.492 ± 0.016	1.004TyrPro: 1.004 ± 0.023	0.651TyrGln: 0.651 ± 0.022	1.848TyrArg: 1.848 ± 0.035	0.984TyrSer: 0.984 ± 0.024	0.975TyrThr: 0.975 ± 0.027	1.469TyrVal: 1.469 ± 0.032	0.338TyrTrp: 0.338 ± 0.015	0.502TyrTyr: 0.502 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5547 proteins (1799258 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---