Bombyx mandarina (Wild silk moth) (Wild silkworm)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Amphiesmenoptera; Lepidoptera; Glossata;

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17282 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J2JRF6|A0A6J2JRF6_BOMMA 28S ribosomal protein S11 mitochondrial OS=Bombyx mandarina OX=7092 GN=LOC114244065 PE=3 SV=1
MM1 pKa = 8.23AEE3 pKa = 4.01EE4 pKa = 4.48RR5 pKa = 11.84RR6 pKa = 11.84LGSDD10 pKa = 3.96GLPGVQDD17 pKa = 3.63NPGGVYY23 pKa = 10.22VADD26 pKa = 4.7DD27 pKa = 3.83NPGGVHH33 pKa = 6.64IVDD36 pKa = 3.8NNNPGGVYY44 pKa = 10.19VVDD47 pKa = 4.68DD48 pKa = 4.37NPGGVYY54 pKa = 10.21VVDD57 pKa = 4.68DD58 pKa = 4.37NPGGVYY64 pKa = 10.21VVDD67 pKa = 4.68DD68 pKa = 4.37NPGGVYY74 pKa = 10.21VVDD77 pKa = 4.64DD78 pKa = 4.17NPGGVHH84 pKa = 6.57IVDD87 pKa = 4.62DD88 pKa = 4.43NPGGVHH94 pKa = 6.37IVDD97 pKa = 3.37NTEE100 pKa = 3.52RR101 pKa = 11.84AINIVEE107 pKa = 3.92

Molecular weight: 11.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J2JZ13|A0A6J2JZ13_BOMMA collagenase-like OS=Bombyx mandarina OX=7092 GN=LOC114246500 PE=3 SV=1
MM1 pKa = 7.44SAHH4 pKa = 6.1KK5 pKa = 9.54TFIIKK10 pKa = 10.29RR11 pKa = 11.84KK12 pKa = 8.65LAKK15 pKa = 9.89KK16 pKa = 10.19LKK18 pKa = 8.64QNRR21 pKa = 11.84PIPQWVRR28 pKa = 11.84MRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.47RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.1WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.89LKK50 pKa = 10.55LL51 pKa = 3.39

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12763

4519

17282

10885424

34

15525

629.9

70.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.262 ± 0.029

1.975 ± 0.021

5.593 ± 0.013

6.846 ± 0.031

3.383 ± 0.015

5.674 ± 0.025

2.535 ± 0.012

5.128 ± 0.02

6.222 ± 0.031

9.012 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.146 ± 0.01

4.649 ± 0.017

5.631 ± 0.023

4.031 ± 0.018

5.875 ± 0.021

7.846 ± 0.027

5.8 ± 0.017

6.258 ± 0.014

1.081 ± 0.006

3.048 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski