Trichinella nelsoni
Average proteome isoelectric point is 7.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16597 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0V0RTX7|A0A0V0RTX7_9BILA Cytosolic Fe-S cluster assembly factor NARFL OS=Trichinella nelsoni OX=6336 GN=NARFL PE=3 SV=1
MM1 pKa = 7.52 VDD3 pKa = 3.19 QKK5 pKa = 11.31 FFLFNFPFGNSCIAAFVCFQCLRR28 pKa = 11.84 HH29 pKa = 6.14 QLADD33 pKa = 4.21 WIDD36 pKa = 4.39 DD37 pKa = 4.54 DD38 pKa = 6.85 DD39 pKa = 7.24 DD40 pKa = 6.92 DD41 pKa = 5.51 DD42 pKa = 6.94 ASVEE46 pKa = 4.4 QNIGWDD52 pKa = 3.51 AYY54 pKa = 10.72 ALSDD58 pKa = 3.86 NKK60 pKa = 10.89 SDD62 pKa = 4.06 FLFEE66 pKa = 5.37 RR67 pKa = 11.84 PIQLQLCCC75 pKa = 5.0
Molecular weight: 8.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.719
IPC_protein 3.694
Toseland 3.465
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.732
Rodwell 3.528
Grimsley 3.376
Solomon 3.694
Lehninger 3.656
Nozaki 3.859
DTASelect 4.151
Thurlkill 3.554
EMBOSS 3.732
Sillero 3.834
Patrickios 0.769
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.744
Protein with the highest isoelectric point:
>tr|A0A0V0RFX1|A0A0V0RFX1_9BILA Uncharacterized protein OS=Trichinella nelsoni OX=6336 GN=T07_3212 PE=4 SV=1
MM1 pKa = 7.25 ARR3 pKa = 11.84 SARR6 pKa = 11.84 SYY8 pKa = 10.11 SRR10 pKa = 11.84 SRR12 pKa = 11.84 SRR14 pKa = 11.84 SSTRR18 pKa = 11.84 RR19 pKa = 11.84 SMSSRR24 pKa = 11.84 RR25 pKa = 11.84 GSHH28 pKa = 3.8 TRR30 pKa = 11.84 SRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 SISRR38 pKa = 11.84 IQTPKK43 pKa = 10.6 RR44 pKa = 11.84 STHH47 pKa = 5.63 QIVVPSSRR55 pKa = 11.84 LKK57 pKa = 9.53 LQKK60 pKa = 10.06 RR61 pKa = 11.84 QSAVPLNGGEE71 pKa = 4.09 APSEE75 pKa = 4.16 IICPSCLQSLRR86 pKa = 11.84 SRR88 pKa = 11.84 SRR90 pKa = 11.84 SKK92 pKa = 10.85 SITRR96 pKa = 11.84 KK97 pKa = 9.68 KK98 pKa = 10.39 SPGRR102 pKa = 11.84 KK103 pKa = 8.41 GARR106 pKa = 11.84 KK107 pKa = 8.39 VAVRR111 pKa = 11.84 QRR113 pKa = 11.84 SKK115 pKa = 11.24 SRR117 pKa = 11.84 GKK119 pKa = 9.89 SRR121 pKa = 11.84 RR122 pKa = 11.84 NKK124 pKa = 9.54 KK125 pKa = 10.13 RR126 pKa = 11.84 SLKK129 pKa = 10.41 KK130 pKa = 10.13 SNQKK134 pKa = 10.45 AVLIKK139 pKa = 10.45 ASKK142 pKa = 9.65 SRR144 pKa = 11.84 RR145 pKa = 11.84 KK146 pKa = 9.76 RR147 pKa = 11.84 SSLKK151 pKa = 10.56 SIPKK155 pKa = 8.38 QKK157 pKa = 10.2 RR158 pKa = 11.84 YY159 pKa = 8.87 SLRR162 pKa = 11.84 SRR164 pKa = 11.84 AAVRR168 pKa = 3.42
Molecular weight: 19.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.008
IPC_protein 12.457
Toseland 12.632
ProMoST 13.115
Dawson 12.632
Bjellqvist 12.618
Wikipedia 13.1
Rodwell 12.354
Grimsley 12.676
Solomon 13.115
Lehninger 13.027
Nozaki 12.632
DTASelect 12.618
Thurlkill 12.632
EMBOSS 13.13
Sillero 12.632
Patrickios 12.062
IPC_peptide 13.13
IPC2_peptide 12.106
IPC2.peptide.svr19 9.058
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13005
3592
16597
8169530
30
16931
492.2
55.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.417 ± 0.017
2.624 ± 0.018
5.253 ± 0.015
6.339 ± 0.02
4.605 ± 0.014
4.978 ± 0.021
2.453 ± 0.007
5.544 ± 0.017
5.998 ± 0.02
9.713 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.406 ± 0.008
5.005 ± 0.013
4.346 ± 0.019
4.267 ± 0.013
5.62 ± 0.016
8.373 ± 0.022
5.301 ± 0.011
6.45 ± 0.013
1.213 ± 0.006
3.092 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here