Celeribacter neptunius
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4197 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I3TEJ0|A0A1I3TEJ0_9RHOB ABC-2 type transport system permease protein OS=Celeribacter neptunius OX=588602 GN=SAMN04487991_2693 PE=4 SV=1
MM1 pKa = 7.19 SAIEE5 pKa = 3.99 FSEE8 pKa = 4.09 NGTAVMFCSHH18 pKa = 7.08 ILSGGRR24 pKa = 11.84 MTKK27 pKa = 10.56 YY28 pKa = 9.71 KK29 pKa = 11.16 GEE31 pKa = 4.23 VASCAPALVRR41 pKa = 11.84 PSVVFPRR48 pKa = 11.84 LFSAPTVSALALAAGLSVAPVLLPSEE74 pKa = 4.28 ITGGAGAAWAACSPSNAGTAGDD96 pKa = 5.84 DD97 pKa = 5.2 IILCDD102 pKa = 3.99 AANDD106 pKa = 4.07 PGGANVSGFTGNDD119 pKa = 3.6 TITLDD124 pKa = 4.06 GMVTVGNISGGDD136 pKa = 3.53 GVDD139 pKa = 3.93 TINLLSGTVINRR151 pKa = 11.84 VVWGNGDD158 pKa = 3.34 EE159 pKa = 4.39 SQILDD164 pKa = 3.84 LTNVTLAGDD173 pKa = 3.68 LRR175 pKa = 11.84 FGGGSDD181 pKa = 3.44 NFTLVAGSVGSNVFLGDD198 pKa = 3.89 LEE200 pKa = 4.49 NFGTGVDD207 pKa = 4.77 DD208 pKa = 5.26 VFTLDD213 pKa = 3.63 GGHH216 pKa = 5.89 VAWEE220 pKa = 3.78 ISGDD224 pKa = 3.66 QDD226 pKa = 3.58 RR227 pKa = 11.84 DD228 pKa = 3.75 TINIYY233 pKa = 10.24 SGSVGRR239 pKa = 11.84 RR240 pKa = 11.84 IKK242 pKa = 10.82 GYY244 pKa = 10.84 DD245 pKa = 3.44 GDD247 pKa = 6.0 DD248 pKa = 3.69 VINWSGGTIGIGVYY262 pKa = 10.86 GDD264 pKa = 3.68 GDD266 pKa = 3.79 WYY268 pKa = 10.62 DD269 pKa = 3.73 GLDD272 pKa = 3.27 WFPTYY277 pKa = 10.92 GSDD280 pKa = 3.22 TVTVSASEE288 pKa = 4.01 YY289 pKa = 10.8 DD290 pKa = 3.57 GTQMLDD296 pKa = 3.46 GGDD299 pKa = 3.76 DD300 pKa = 3.98 AYY302 pKa = 10.11 TSDD305 pKa = 4.86 GYY307 pKa = 11.71 SDD309 pKa = 3.6 TLNLNGLTVSANGTYY324 pKa = 9.58 IVNWEE329 pKa = 4.2 VANLDD334 pKa = 3.46 ATALTISDD342 pKa = 4.2 GLWDD346 pKa = 3.78 VGIDD350 pKa = 3.4 GATGSGVFLTNGSEE364 pKa = 4.68 LEE366 pKa = 4.18 ALDD369 pKa = 5.19 ALTLNGNLQIDD380 pKa = 4.26 GTSQFTGTGAGAGVYY395 pKa = 9.93 NINGTVLNSGTITTYY410 pKa = 11.11 DD411 pKa = 3.47 GVTGDD416 pKa = 3.9 VLTISGDD423 pKa = 3.65 YY424 pKa = 11.12 SGDD427 pKa = 3.53 GQLLFDD433 pKa = 5.55 ADD435 pKa = 4.31 LASGTGDD442 pKa = 3.45 ALVIDD447 pKa = 4.66 GDD449 pKa = 4.23 VTGGTTTIMVNDD461 pKa = 3.92 VTSGPISGNDD471 pKa = 3.24 VVLVDD476 pKa = 3.59 VTGATAEE483 pKa = 4.29 GDD485 pKa = 3.71 FVLAGGPLTVGAYY498 pKa = 10.03 DD499 pKa = 3.71 YY500 pKa = 11.65 DD501 pKa = 5.37 LIYY504 pKa = 10.91 QPGTFVLAGSLNSIGAMYY522 pKa = 9.58 EE523 pKa = 3.79 AAPLILAGFNRR534 pKa = 11.84 LPTLEE539 pKa = 3.9 QRR541 pKa = 11.84 VGEE544 pKa = 4.21 RR545 pKa = 11.84 EE546 pKa = 4.09 LTGSDD551 pKa = 3.21 TASGSWIRR559 pKa = 11.84 FYY561 pKa = 11.56 GDD563 pKa = 3.14 SSDD566 pKa = 3.4 MATNSGSNISDD577 pKa = 3.64 HH578 pKa = 6.98 RR579 pKa = 11.84 WGLQAGWDD587 pKa = 3.68 EE588 pKa = 3.98 RR589 pKa = 11.84 LEE591 pKa = 4.18 PSEE594 pKa = 3.99 TGQWVIGVTAQYY606 pKa = 7.98 GTQNASVSMASGAGKK621 pKa = 10.37 VSSSNYY627 pKa = 10.09 GIGATATWYY636 pKa = 11.06 GNDD639 pKa = 3.65 GLYY642 pKa = 10.71 ADD644 pKa = 4.92 LQGQVSWISSDD655 pKa = 3.56 FSSSSVGVLATGEE668 pKa = 4.1 RR669 pKa = 11.84 ANAYY673 pKa = 8.67 AASIEE678 pKa = 4.15 TGYY681 pKa = 10.96 RR682 pKa = 11.84 FALDD686 pKa = 3.06 QSRR689 pKa = 11.84 ALVPQAQMSQGYY701 pKa = 9.55 VGGAAFTDD709 pKa = 3.9 SQGNAIDD716 pKa = 4.58 LGSSSGLTGRR726 pKa = 11.84 LGLAYY731 pKa = 9.63 EE732 pKa = 4.43 YY733 pKa = 10.77 EE734 pKa = 4.48 RR735 pKa = 11.84 SDD737 pKa = 4.0 DD738 pKa = 3.74 AAGGHH743 pKa = 6.32 AKK745 pKa = 10.34 LYY747 pKa = 10.83 AIGNLLHH754 pKa = 7.12 DD755 pKa = 4.75 FSEE758 pKa = 5.26 GSTVQVAGLDD768 pKa = 3.99 LNEE771 pKa = 3.93 GTARR775 pKa = 11.84 NWGEE779 pKa = 3.84 VGIGTSFASDD789 pKa = 4.02 EE790 pKa = 3.96 NTMFYY795 pKa = 11.36 GEE797 pKa = 3.92 ASYY800 pKa = 8.64 RR801 pKa = 11.84 TALDD805 pKa = 4.34 GPDD808 pKa = 3.22 GTNNGFSATAGLRR821 pKa = 11.84 INWW824 pKa = 3.54
Molecular weight: 84.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.716
IPC2_protein 3.617
IPC_protein 3.668
Toseland 3.427
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.821
Wikipedia 3.63
Rodwell 3.49
Grimsley 3.338
Solomon 3.668
Lehninger 3.63
Nozaki 3.783
DTASelect 4.075
Thurlkill 3.49
EMBOSS 3.63
Sillero 3.795
Patrickios 0.731
IPC_peptide 3.668
IPC2_peptide 3.77
IPC2.peptide.svr19 3.72
Protein with the highest isoelectric point:
>tr|A0A1I3RB13|A0A1I3RB13_9RHOB Nitrous oxidase accessory protein OS=Celeribacter neptunius OX=588602 GN=SAMN04487991_2114 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.44 AGRR28 pKa = 11.84 KK29 pKa = 8.46 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4197
0
4197
1294092
32
1511
308.3
33.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.893 ± 0.05
0.865 ± 0.012
5.882 ± 0.035
6.389 ± 0.037
3.841 ± 0.025
8.584 ± 0.036
2.073 ± 0.017
5.292 ± 0.026
3.553 ± 0.027
10.17 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.891 ± 0.02
2.557 ± 0.018
5.001 ± 0.026
3.088 ± 0.021
6.279 ± 0.035
5.399 ± 0.025
5.522 ± 0.03
7.066 ± 0.026
1.343 ± 0.015
2.312 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here