Citrus unshiu (Satsuma mandarin) (Citrus nobilis var. unshiu)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35792 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5QCU2|A0A2H5QCU2_CITUN Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_217830 PE=4 SV=1
MM1 pKa = 7.63DD2 pKa = 3.53QYY4 pKa = 11.29HH5 pKa = 5.77YY6 pKa = 9.97HH7 pKa = 6.06TVQNHH12 pKa = 5.22PVQNQSSDD20 pKa = 3.18SSEE23 pKa = 4.14PEE25 pKa = 3.98TVDD28 pKa = 3.12EE29 pKa = 4.94SNPSEE34 pKa = 4.27PNPSSSNSSTTSDD47 pKa = 3.29GTSTDD52 pKa = 3.58SEE54 pKa = 4.54SEE56 pKa = 4.18YY57 pKa = 11.54ADD59 pKa = 2.87ITGILMATEE68 pKa = 4.1TADD71 pKa = 3.81PNASTSTPIVEE82 pKa = 4.83DD83 pKa = 3.61QPSDD87 pKa = 3.49QASQTDD93 pKa = 3.93PMPPPVHH100 pKa = 6.03EE101 pKa = 5.36HH102 pKa = 5.2PTKK105 pKa = 10.48PSSTSS110 pKa = 2.93

Molecular weight: 11.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5N0U6|A0A2H5N0U6_CITUN Isoform of A0A2H5MZY1 Uncharacterized protein (Fragment) OS=Citrus unshiu OX=55188 GN=CUMW_285610 PE=4 SV=1
MM1 pKa = 7.13VNRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84WWGMVNWSWRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84LIFVRR24 pKa = 11.84FLWWWWGMVNRR35 pKa = 11.84SWWRR39 pKa = 11.84GRR41 pKa = 11.84LVFVWFLWWWW51 pKa = 3.34

Molecular weight: 7.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

28786

7006

35792

14900710

8

6153

416.3

46.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.594 ± 0.013

1.945 ± 0.007

5.179 ± 0.008

6.323 ± 0.013

4.388 ± 0.008

6.388 ± 0.011

2.344 ± 0.005

5.567 ± 0.009

6.077 ± 0.012

10.221 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.405 ± 0.005

4.724 ± 0.01

4.696 ± 0.01

3.647 ± 0.008

5.164 ± 0.01

8.938 ± 0.014

4.792 ± 0.007

6.461 ± 0.01

1.299 ± 0.004

2.836 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski