Microbacterium phage Hyperion

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Squashvirus; Microbacterium virus Hyperion

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 104 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8UIW7|A0A2U8UIW7_9CAUD Lysin A OS=Microbacterium phage Hyperion OX=2182354 GN=45 PE=4 SV=1
MM1 pKa = 6.71EE2 pKa = 4.48TCATDD7 pKa = 3.26AAGICLTHH15 pKa = 7.28GNDD18 pKa = 5.03CDD20 pKa = 3.87DD21 pKa = 3.89TLLFLTDD28 pKa = 3.52HH29 pKa = 6.87

Molecular weight: 3.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8UIN9|A0A2U8UIN9_9CAUD Terminase OS=Microbacterium phage Hyperion OX=2182354 GN=18 PE=4 SV=1
MM1 pKa = 7.75AKK3 pKa = 9.59QEE5 pKa = 4.24RR6 pKa = 11.84PRR8 pKa = 11.84YY9 pKa = 6.64TPPRR13 pKa = 11.84LGQRR17 pKa = 11.84DD18 pKa = 3.91FARR21 pKa = 11.84TTMMPRR27 pKa = 11.84WFTRR31 pKa = 11.84EE32 pKa = 3.6AVEE35 pKa = 4.88AFGLNPYY42 pKa = 10.38DD43 pKa = 3.56CTTFAIIADD52 pKa = 4.02NLDD55 pKa = 3.57AAGVSQTATTLIASRR70 pKa = 11.84GGMSRR75 pKa = 11.84GAAARR80 pKa = 11.84AVDD83 pKa = 4.32RR84 pKa = 11.84LIANQLLFEE93 pKa = 5.58LDD95 pKa = 3.38PRR97 pKa = 11.84KK98 pKa = 9.93NGHH101 pKa = 4.93MMRR104 pKa = 11.84YY105 pKa = 9.52AIPADD110 pKa = 3.93LPWTGGRR117 pKa = 11.84RR118 pKa = 3.47

Molecular weight: 13.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

104

0

104

19838

27

1104

190.8

20.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.705 ± 0.351

0.812 ± 0.113

6.543 ± 0.227

6.649 ± 0.337

2.692 ± 0.142

8.67 ± 0.24

1.744 ± 0.153

5.001 ± 0.185

2.697 ± 0.213

7.511 ± 0.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.283 ± 0.129

2.929 ± 0.166

5.605 ± 0.409

3.68 ± 0.372

6.987 ± 0.286

5.484 ± 0.295

7.103 ± 0.35

7.481 ± 0.192

1.966 ± 0.159

2.46 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski