Amino acid dipepetide frequency for Microbacterium phage Hyperion

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.341AlaAla: 17.341 ± 1.643
0.403AlaCys: 0.403 ± 0.153
7.814AlaAsp: 7.814 ± 0.802
7.31AlaGlu: 7.31 ± 0.826
2.47AlaPhe: 2.47 ± 0.392
8.822AlaGly: 8.822 ± 0.953
1.865AlaHis: 1.865 ± 0.336
6.301AlaIle: 6.301 ± 0.532
4.083AlaLys: 4.083 ± 0.519
10.032AlaLeu: 10.032 ± 0.929
2.621AlaMet: 2.621 ± 0.416
3.125AlaAsn: 3.125 ± 0.409
5.747AlaPro: 5.747 ± 0.665
4.134AlaGln: 4.134 ± 0.46
7.814AlaArg: 7.814 ± 0.573
5.646AlaSer: 5.646 ± 0.815
8.116AlaThr: 8.116 ± 0.895
8.519AlaVal: 8.519 ± 0.83
1.916AlaTrp: 1.916 ± 0.29
2.924AlaTyr: 2.924 ± 0.374
0.0AlaXaa: 0.0 ± 0.0
Cys
0.756CysAla: 0.756 ± 0.211
0.05CysCys: 0.05 ± 0.054
0.655CysAsp: 0.655 ± 0.225
0.655CysGlu: 0.655 ± 0.187
0.353CysPhe: 0.353 ± 0.18
1.059CysGly: 1.059 ± 0.248
0.151CysHis: 0.151 ± 0.075
0.252CysIle: 0.252 ± 0.117
0.05CysLys: 0.05 ± 0.052
0.555CysLeu: 0.555 ± 0.2
0.05CysMet: 0.05 ± 0.051
0.151CysAsn: 0.151 ± 0.097
0.756CysPro: 0.756 ± 0.177
0.252CysGln: 0.252 ± 0.115
0.555CysArg: 0.555 ± 0.193
0.302CysSer: 0.302 ± 0.135
0.605CysThr: 0.605 ± 0.199
0.605CysVal: 0.605 ± 0.172
0.151CysTrp: 0.151 ± 0.088
0.151CysTyr: 0.151 ± 0.076
0.0CysXaa: 0.0 ± 0.0
Asp
8.217AspAla: 8.217 ± 0.795
0.706AspCys: 0.706 ± 0.158
4.587AspAsp: 4.587 ± 0.527
4.638AspGlu: 4.638 ± 0.567
2.168AspPhe: 2.168 ± 0.34
7.259AspGly: 7.259 ± 0.722
1.412AspHis: 1.412 ± 0.299
2.722AspIle: 2.722 ± 0.415
1.462AspLys: 1.462 ± 0.322
5.243AspLeu: 5.243 ± 0.564
1.311AspMet: 1.311 ± 0.23
1.563AspAsn: 1.563 ± 0.311
4.083AspPro: 4.083 ± 0.406
2.722AspGln: 2.722 ± 0.354
3.982AspArg: 3.982 ± 0.42
2.621AspSer: 2.621 ± 0.408
3.125AspThr: 3.125 ± 0.316
4.487AspVal: 4.487 ± 0.499
1.512AspTrp: 1.512 ± 0.29
1.613AspTyr: 1.613 ± 0.477
0.0AspXaa: 0.0 ± 0.0
Glu
7.31GluAla: 7.31 ± 0.637
0.454GluCys: 0.454 ± 0.174
3.882GluAsp: 3.882 ± 0.475
4.285GluGlu: 4.285 ± 0.523
1.966GluPhe: 1.966 ± 0.29
4.739GluGly: 4.739 ± 0.558
1.412GluHis: 1.412 ± 0.314
3.831GluIle: 3.831 ± 0.49
2.168GluLys: 2.168 ± 0.449
5.495GluLeu: 5.495 ± 0.625
1.815GluMet: 1.815 ± 0.282
2.168GluAsn: 2.168 ± 0.35
4.033GluPro: 4.033 ± 0.698
3.529GluGln: 3.529 ± 0.559
5.797GluArg: 5.797 ± 0.576
2.369GluSer: 2.369 ± 0.364
3.025GluThr: 3.025 ± 0.409
5.091GluVal: 5.091 ± 0.562
1.512GluTrp: 1.512 ± 0.317
1.613GluTyr: 1.613 ± 0.281
0.0GluXaa: 0.0 ± 0.0
Phe
2.722PheAla: 2.722 ± 0.434
0.353PheCys: 0.353 ± 0.127
2.722PheAsp: 2.722 ± 0.415
1.865PheGlu: 1.865 ± 0.309
0.202PhePhe: 0.202 ± 0.081
3.125PheGly: 3.125 ± 0.458
0.504PheHis: 0.504 ± 0.182
1.109PheIle: 1.109 ± 0.259
0.655PheLys: 0.655 ± 0.18
1.815PheLeu: 1.815 ± 0.314
0.454PheMet: 0.454 ± 0.164
0.302PheAsn: 0.302 ± 0.112
1.159PhePro: 1.159 ± 0.273
0.605PheGln: 0.605 ± 0.173
2.016PheArg: 2.016 ± 0.365
2.168PheSer: 2.168 ± 0.323
2.369PheThr: 2.369 ± 0.365
1.311PheVal: 1.311 ± 0.221
0.202PheTrp: 0.202 ± 0.128
1.26PheTyr: 1.26 ± 0.277
0.0PheXaa: 0.0 ± 0.0
Gly
7.36GlyAla: 7.36 ± 0.736
0.857GlyCys: 0.857 ± 0.235
5.948GlyAsp: 5.948 ± 0.597
5.394GlyGlu: 5.394 ± 0.642
3.025GlyPhe: 3.025 ± 0.467
7.713GlyGly: 7.713 ± 0.695
1.563GlyHis: 1.563 ± 0.371
2.924GlyIle: 2.924 ± 0.41
3.63GlyLys: 3.63 ± 0.485
6.251GlyLeu: 6.251 ± 0.598
2.42GlyMet: 2.42 ± 0.361
2.369GlyAsn: 2.369 ± 0.378
3.327GlyPro: 3.327 ± 0.417
2.873GlyGln: 2.873 ± 0.504
6.453GlyArg: 6.453 ± 0.724
5.444GlySer: 5.444 ± 0.633
6.705GlyThr: 6.705 ± 0.981
7.915GlyVal: 7.915 ± 0.579
2.016GlyTrp: 2.016 ± 0.332
2.521GlyTyr: 2.521 ± 0.353
0.0GlyXaa: 0.0 ± 0.0
His
2.067HisAla: 2.067 ± 0.38
0.202HisCys: 0.202 ± 0.104
0.907HisAsp: 0.907 ± 0.268
1.412HisGlu: 1.412 ± 0.356
0.655HisPhe: 0.655 ± 0.169
1.26HisGly: 1.26 ± 0.242
0.756HisHis: 0.756 ± 0.223
0.807HisIle: 0.807 ± 0.264
0.252HisLys: 0.252 ± 0.117
1.664HisLeu: 1.664 ± 0.342
0.302HisMet: 0.302 ± 0.131
0.504HisAsn: 0.504 ± 0.159
1.311HisPro: 1.311 ± 0.284
0.403HisGln: 0.403 ± 0.168
1.512HisArg: 1.512 ± 0.325
0.605HisSer: 0.605 ± 0.17
0.655HisThr: 0.655 ± 0.198
1.361HisVal: 1.361 ± 0.294
0.302HisTrp: 0.302 ± 0.126
0.504HisTyr: 0.504 ± 0.175
0.0HisXaa: 0.0 ± 0.0
Ile
6.553IleAla: 6.553 ± 0.591
0.353IleCys: 0.353 ± 0.135
3.579IleAsp: 3.579 ± 0.467
3.63IleGlu: 3.63 ± 0.476
1.462IlePhe: 1.462 ± 0.272
4.638IleGly: 4.638 ± 0.513
0.907IleHis: 0.907 ± 0.229
2.924IleIle: 2.924 ± 0.423
0.706IleLys: 0.706 ± 0.24
2.571IleLeu: 2.571 ± 0.36
0.857IleMet: 0.857 ± 0.237
1.462IleAsn: 1.462 ± 0.248
2.722IlePro: 2.722 ± 0.459
1.109IleGln: 1.109 ± 0.204
3.327IleArg: 3.327 ± 0.386
2.823IleSer: 2.823 ± 0.342
4.638IleThr: 4.638 ± 0.483
3.882IleVal: 3.882 ± 0.413
0.958IleTrp: 0.958 ± 0.216
0.907IleTyr: 0.907 ± 0.218
0.0IleXaa: 0.0 ± 0.0
Lys
3.68LysAla: 3.68 ± 0.552
0.202LysCys: 0.202 ± 0.139
1.412LysAsp: 1.412 ± 0.236
1.815LysGlu: 1.815 ± 0.294
1.059LysPhe: 1.059 ± 0.184
1.764LysGly: 1.764 ± 0.246
0.504LysHis: 0.504 ± 0.177
1.059LysIle: 1.059 ± 0.251
0.252LysLys: 0.252 ± 0.115
2.773LysLeu: 2.773 ± 0.423
0.807LysMet: 0.807 ± 0.2
1.109LysAsn: 1.109 ± 0.211
1.512LysPro: 1.512 ± 0.285
1.059LysGln: 1.059 ± 0.187
1.664LysArg: 1.664 ± 0.286
1.664LysSer: 1.664 ± 0.301
1.865LysThr: 1.865 ± 0.408
1.865LysVal: 1.865 ± 0.327
0.353LysTrp: 0.353 ± 0.139
0.555LysTyr: 0.555 ± 0.203
0.0LysXaa: 0.0 ± 0.0
Leu
10.586LeuAla: 10.586 ± 0.746
0.202LeuCys: 0.202 ± 0.096
5.142LeuAsp: 5.142 ± 0.512
5.495LeuGlu: 5.495 ± 0.58
1.613LeuPhe: 1.613 ± 0.305
6.352LeuGly: 6.352 ± 0.588
0.958LeuHis: 0.958 ± 0.247
3.63LeuIle: 3.63 ± 0.467
2.067LeuLys: 2.067 ± 0.366
5.444LeuLeu: 5.444 ± 0.584
1.21LeuMet: 1.21 ± 0.222
3.277LeuAsn: 3.277 ± 0.485
3.378LeuPro: 3.378 ± 0.394
1.916LeuGln: 1.916 ± 0.288
5.495LeuArg: 5.495 ± 0.494
4.94LeuSer: 4.94 ± 0.651
6.049LeuThr: 6.049 ± 0.537
4.638LeuVal: 4.638 ± 0.506
1.26LeuTrp: 1.26 ± 0.28
1.462LeuTyr: 1.462 ± 0.266
0.0LeuXaa: 0.0 ± 0.0
Met
2.924MetAla: 2.924 ± 0.422
0.202MetCys: 0.202 ± 0.106
1.008MetAsp: 1.008 ± 0.235
0.655MetGlu: 0.655 ± 0.159
0.555MetPhe: 0.555 ± 0.168
1.664MetGly: 1.664 ± 0.295
0.454MetHis: 0.454 ± 0.161
1.26MetIle: 1.26 ± 0.282
0.756MetLys: 0.756 ± 0.172
2.067MetLeu: 2.067 ± 0.305
0.454MetMet: 0.454 ± 0.194
0.555MetAsn: 0.555 ± 0.152
1.714MetPro: 1.714 ± 0.306
0.857MetGln: 0.857 ± 0.184
1.714MetArg: 1.714 ± 0.29
2.369MetSer: 2.369 ± 0.413
1.563MetThr: 1.563 ± 0.26
1.664MetVal: 1.664 ± 0.297
0.302MetTrp: 0.302 ± 0.132
0.101MetTyr: 0.101 ± 0.078
0.0MetXaa: 0.0 ± 0.0
Asn
3.378AsnAla: 3.378 ± 0.368
0.202AsnCys: 0.202 ± 0.085
1.563AsnAsp: 1.563 ± 0.28
1.764AsnGlu: 1.764 ± 0.264
0.504AsnPhe: 0.504 ± 0.215
3.428AsnGly: 3.428 ± 0.459
0.403AsnHis: 0.403 ± 0.189
1.311AsnIle: 1.311 ± 0.21
0.504AsnLys: 0.504 ± 0.13
2.016AsnLeu: 2.016 ± 0.33
0.403AsnMet: 0.403 ± 0.147
0.655AsnAsn: 0.655 ± 0.224
2.47AsnPro: 2.47 ± 0.557
1.613AsnGln: 1.613 ± 0.287
1.613AsnArg: 1.613 ± 0.311
1.815AsnSer: 1.815 ± 0.274
2.571AsnThr: 2.571 ± 0.449
1.613AsnVal: 1.613 ± 0.299
0.756AsnTrp: 0.756 ± 0.193
0.706AsnTyr: 0.706 ± 0.227
0.0AsnXaa: 0.0 ± 0.0
Pro
6.755ProAla: 6.755 ± 1.002
0.504ProCys: 0.504 ± 0.195
3.478ProAsp: 3.478 ± 0.561
4.789ProGlu: 4.789 ± 0.583
1.109ProPhe: 1.109 ± 0.25
4.89ProGly: 4.89 ± 0.71
0.807ProHis: 0.807 ± 0.194
2.672ProIle: 2.672 ± 0.444
1.311ProLys: 1.311 ± 0.286
3.428ProLeu: 3.428 ± 0.341
0.807ProMet: 0.807 ± 0.198
1.966ProAsn: 1.966 ± 0.321
2.571ProPro: 2.571 ± 0.346
2.067ProGln: 2.067 ± 0.613
3.68ProArg: 3.68 ± 0.551
3.579ProSer: 3.579 ± 0.436
4.739ProThr: 4.739 ± 0.764
4.335ProVal: 4.335 ± 0.487
1.109ProTrp: 1.109 ± 0.224
1.462ProTyr: 1.462 ± 0.336
0.0ProXaa: 0.0 ± 0.0
Gln
4.587GlnAla: 4.587 ± 0.608
0.454GlnCys: 0.454 ± 0.179
1.159GlnAsp: 1.159 ± 0.243
1.966GlnGlu: 1.966 ± 0.359
0.958GlnPhe: 0.958 ± 0.219
3.176GlnGly: 3.176 ± 0.716
0.807GlnHis: 0.807 ± 0.187
2.168GlnIle: 2.168 ± 0.397
0.807GlnLys: 0.807 ± 0.221
2.369GlnLeu: 2.369 ± 0.273
1.21GlnMet: 1.21 ± 0.275
0.857GlnAsn: 0.857 ± 0.195
2.47GlnPro: 2.47 ± 0.62
3.025GlnGln: 3.025 ± 1.516
2.823GlnArg: 2.823 ± 0.365
1.865GlnSer: 1.865 ± 0.32
1.664GlnThr: 1.664 ± 0.24
2.571GlnVal: 2.571 ± 0.407
0.958GlnTrp: 0.958 ± 0.209
0.907GlnTyr: 0.907 ± 0.266
0.0GlnXaa: 0.0 ± 0.0
Arg
8.217ArgAla: 8.217 ± 0.61
0.857ArgCys: 0.857 ± 0.21
4.386ArgAsp: 4.386 ± 0.556
4.638ArgGlu: 4.638 ± 0.61
1.815ArgPhe: 1.815 ± 0.248
5.142ArgGly: 5.142 ± 0.6
1.109ArgHis: 1.109 ± 0.271
3.68ArgIle: 3.68 ± 0.495
2.621ArgLys: 2.621 ± 0.341
4.739ArgLeu: 4.739 ± 0.502
2.521ArgMet: 2.521 ± 0.387
2.067ArgAsn: 2.067 ± 0.31
2.42ArgPro: 2.42 ± 0.375
2.521ArgGln: 2.521 ± 0.333
6.553ArgArg: 6.553 ± 0.684
4.033ArgSer: 4.033 ± 0.449
3.882ArgThr: 3.882 ± 0.346
5.192ArgVal: 5.192 ± 0.587
1.21ArgTrp: 1.21 ± 0.267
2.268ArgTyr: 2.268 ± 0.311
0.0ArgXaa: 0.0 ± 0.0
Ser
6.201SerAla: 6.201 ± 0.836
0.353SerCys: 0.353 ± 0.141
3.378SerAsp: 3.378 ± 0.406
3.63SerGlu: 3.63 ± 0.508
1.311SerPhe: 1.311 ± 0.246
4.789SerGly: 4.789 ± 0.544
0.958SerHis: 0.958 ± 0.278
2.773SerIle: 2.773 ± 0.323
0.907SerLys: 0.907 ± 0.196
4.688SerLeu: 4.688 ± 0.576
1.664SerMet: 1.664 ± 0.275
1.512SerAsn: 1.512 ± 0.281
3.075SerPro: 3.075 ± 0.402
1.664SerGln: 1.664 ± 0.276
3.579SerArg: 3.579 ± 0.485
3.075SerSer: 3.075 ± 0.446
5.091SerThr: 5.091 ± 0.582
3.63SerVal: 3.63 ± 0.42
0.756SerTrp: 0.756 ± 0.222
1.815SerTyr: 1.815 ± 0.378
0.0SerXaa: 0.0 ± 0.0
Thr
7.259ThrAla: 7.259 ± 0.798
0.706ThrCys: 0.706 ± 0.212
4.285ThrAsp: 4.285 ± 0.529
5.142ThrGlu: 5.142 ± 0.48
2.218ThrPhe: 2.218 ± 0.325
6.1ThrGly: 6.1 ± 0.588
1.008ThrHis: 1.008 ± 0.248
3.932ThrIle: 3.932 ± 0.711
1.916ThrLys: 1.916 ± 0.319
6.049ThrLeu: 6.049 ± 0.465
1.512ThrMet: 1.512 ± 0.264
1.563ThrAsn: 1.563 ± 0.312
5.848ThrPro: 5.848 ± 0.81
2.117ThrGln: 2.117 ± 0.293
3.73ThrArg: 3.73 ± 0.505
3.277ThrSer: 3.277 ± 0.43
5.142ThrThr: 5.142 ± 0.738
6.301ThrVal: 6.301 ± 0.783
1.462ThrTrp: 1.462 ± 0.24
1.462ThrTyr: 1.462 ± 0.234
0.0ThrXaa: 0.0 ± 0.0
Val
6.856ValAla: 6.856 ± 0.566
0.605ValCys: 0.605 ± 0.17
6.352ValAsp: 6.352 ± 0.524
4.235ValGlu: 4.235 ± 0.443
1.966ValPhe: 1.966 ± 0.356
6.503ValGly: 6.503 ± 0.722
1.26ValHis: 1.26 ± 0.295
4.739ValIle: 4.739 ± 0.376
1.865ValLys: 1.865 ± 0.397
5.142ValLeu: 5.142 ± 0.525
1.865ValMet: 1.865 ± 0.364
2.621ValAsn: 2.621 ± 0.331
5.091ValPro: 5.091 ± 0.815
2.672ValGln: 2.672 ± 0.438
4.587ValArg: 4.587 ± 0.479
3.73ValSer: 3.73 ± 0.396
5.797ValThr: 5.797 ± 0.798
5.646ValVal: 5.646 ± 0.467
1.664ValTrp: 1.664 ± 0.25
1.613ValTyr: 1.613 ± 0.267
0.0ValXaa: 0.0 ± 0.0
Trp
1.916TrpAla: 1.916 ± 0.333
0.252TrpCys: 0.252 ± 0.114
1.26TrpAsp: 1.26 ± 0.261
1.512TrpGlu: 1.512 ± 0.321
0.857TrpPhe: 0.857 ± 0.222
1.26TrpGly: 1.26 ± 0.277
0.302TrpHis: 0.302 ± 0.12
0.857TrpIle: 0.857 ± 0.222
0.504TrpLys: 0.504 ± 0.153
1.563TrpLeu: 1.563 ± 0.26
0.252TrpMet: 0.252 ± 0.101
0.504TrpAsn: 0.504 ± 0.176
0.907TrpPro: 0.907 ± 0.225
0.706TrpGln: 0.706 ± 0.212
1.109TrpArg: 1.109 ± 0.247
1.159TrpSer: 1.159 ± 0.289
2.067TrpThr: 2.067 ± 0.37
1.462TrpVal: 1.462 ± 0.248
0.756TrpTrp: 0.756 ± 0.22
0.454TrpTyr: 0.454 ± 0.123
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.369TyrAla: 2.369 ± 0.375
0.202TyrCys: 0.202 ± 0.112
1.916TyrAsp: 1.916 ± 0.34
1.664TyrGlu: 1.664 ± 0.282
0.655TyrPhe: 0.655 ± 0.185
3.025TyrGly: 3.025 ± 0.405
0.302TyrHis: 0.302 ± 0.115
1.059TyrIle: 1.059 ± 0.213
0.454TyrLys: 0.454 ± 0.168
1.21TyrLeu: 1.21 ± 0.222
0.252TyrMet: 0.252 ± 0.118
0.958TyrAsn: 0.958 ± 0.212
1.462TyrPro: 1.462 ± 0.304
0.958TyrGln: 0.958 ± 0.188
1.865TyrArg: 1.865 ± 0.313
1.361TyrSer: 1.361 ± 0.161
1.361TyrThr: 1.361 ± 0.251
2.722TyrVal: 2.722 ± 0.424
0.504TyrTrp: 0.504 ± 0.165
0.302TyrTyr: 0.302 ± 0.129
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 104 proteins (19838 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski