Streptococcus phage Javan118

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6API7|A0A4D6API7_9CAUD Uncharacterized protein OS=Streptococcus phage Javan118 OX=2547987 GN=Javan118_0048 PE=4 SV=1
MM1 pKa = 7.26TKK3 pKa = 10.46EE4 pKa = 3.76EE5 pKa = 4.89LIEE8 pKa = 4.52EE9 pKa = 4.3IEE11 pKa = 4.59GEE13 pKa = 4.13INDD16 pKa = 4.18IDD18 pKa = 5.31LLIDD22 pKa = 4.46DD23 pKa = 4.52YY24 pKa = 11.54QYY26 pKa = 11.32EE27 pKa = 4.13IDD29 pKa = 3.99EE30 pKa = 4.41LQRR33 pKa = 11.84DD34 pKa = 3.55IDD36 pKa = 3.89EE37 pKa = 5.03LDD39 pKa = 3.21TRR41 pKa = 11.84KK42 pKa = 10.72DD43 pKa = 3.04ILVRR47 pKa = 11.84KK48 pKa = 8.37LQNLLEE54 pKa = 4.47SVEE57 pKa = 4.29EE58 pKa = 4.15

Molecular weight: 6.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AP70|A0A4D6AP70_9CAUD Uncharacterized protein OS=Streptococcus phage Javan118 OX=2547987 GN=Javan118_0010 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 10.52YY3 pKa = 10.72NYY5 pKa = 9.83NFKK8 pKa = 10.9LNAVNMYY15 pKa = 10.56RR16 pKa = 11.84SGQWIEE22 pKa = 4.13TPVGIGQKK30 pKa = 8.58NFRR33 pKa = 11.84KK34 pKa = 10.21RR35 pKa = 11.84IVRR38 pKa = 11.84WSKK41 pKa = 8.0TAEE44 pKa = 3.4IHH46 pKa = 7.01GFDD49 pKa = 3.89FLKK52 pKa = 10.52HH53 pKa = 5.91SKK55 pKa = 9.87SSKK58 pKa = 9.24NRR60 pKa = 11.84TVEE63 pKa = 3.67EE64 pKa = 4.3RR65 pKa = 11.84YY66 pKa = 10.14EE67 pKa = 3.75LVARR71 pKa = 11.84VFSGEE76 pKa = 4.2SQSSVAINAGIDD88 pKa = 3.25TGLLSKK94 pKa = 9.86WVQIYY99 pKa = 10.04KK100 pKa = 10.04IKK102 pKa = 10.63GYY104 pKa = 10.68EE105 pKa = 3.98GLNLKK110 pKa = 10.2RR111 pKa = 11.84GRR113 pKa = 11.84RR114 pKa = 11.84RR115 pKa = 11.84KK116 pKa = 9.88KK117 pKa = 9.69EE118 pKa = 3.55PPMTKK123 pKa = 9.44KK124 pKa = 10.6YY125 pKa = 10.69KK126 pKa = 10.31PVDD129 pKa = 3.57LTPTEE134 pKa = 4.09RR135 pKa = 11.84EE136 pKa = 3.5EE137 pKa = 4.77LIRR140 pKa = 11.84LRR142 pKa = 11.84AEE144 pKa = 3.55TAYY147 pKa = 11.08LKK149 pKa = 10.03TEE151 pKa = 3.73NEE153 pKa = 4.32AIKK156 pKa = 10.64KK157 pKa = 10.12LIALRR162 pKa = 11.84HH163 pKa = 5.42KK164 pKa = 10.83KK165 pKa = 9.03EE166 pKa = 3.72AALLKK171 pKa = 10.3AKK173 pKa = 10.01KK174 pKa = 9.55RR175 pKa = 11.84QSSRR179 pKa = 11.84NLKK182 pKa = 10.47KK183 pKa = 10.37KK184 pKa = 10.67DD185 pKa = 3.16ITT187 pKa = 3.82

Molecular weight: 21.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

10898

37

785

173.0

19.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.075 ± 0.526

0.569 ± 0.103

6.689 ± 0.386

7.405 ± 0.489

3.927 ± 0.228

6.442 ± 0.434

1.413 ± 0.172

7.102 ± 0.246

9.167 ± 0.445

8.378 ± 0.288

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.386 ± 0.171

5.175 ± 0.185

2.606 ± 0.187

3.799 ± 0.206

4.056 ± 0.331

6.634 ± 0.393

6.203 ± 0.299

6.175 ± 0.2

1.055 ± 0.135

3.744 ± 0.268
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski