Oceanihabitans sp. IOP_32
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2767 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q0JSW9|A0A5Q0JSW9_9FLAO ABC transporter ATP-binding protein OS=Oceanihabitans sp. IOP_32 OX=2529032 GN=FEZ18_02590 PE=4 SV=1
MM1 pKa = 7.17 KK2 pKa = 10.13 TMNNIKK8 pKa = 9.08 TVIVLATLVFSSLLTMSCSSDD29 pKa = 4.89 DD30 pKa = 4.55 DD31 pKa = 4.04 NQDD34 pKa = 4.2 PIVGTWLQVSEE45 pKa = 4.2 LDD47 pKa = 3.72 EE48 pKa = 5.44 RR49 pKa = 11.84 FLNDD53 pKa = 3.57 VPDD56 pKa = 3.74 GTFNQVVDD64 pKa = 4.14 ANNFIRR70 pKa = 11.84 VTFNADD76 pKa = 2.94 GTFTEE81 pKa = 5.72 LYY83 pKa = 10.05 SEE85 pKa = 4.41 SFILNGQEE93 pKa = 4.16 VVEE96 pKa = 4.45 TEE98 pKa = 4.56 AYY100 pKa = 10.1 SGTYY104 pKa = 8.67 TISGSTLTCIEE115 pKa = 4.37 EE116 pKa = 5.0 GYY118 pKa = 10.1 PDD120 pKa = 3.94 DD121 pKa = 4.46 PYY123 pKa = 11.06 IRR125 pKa = 11.84 EE126 pKa = 3.95 FTLNGDD132 pKa = 3.63 TLTLVFTEE140 pKa = 6.18 GYY142 pKa = 8.65 TDD144 pKa = 3.19 QNNDD148 pKa = 2.33 VRR150 pKa = 11.84 RR151 pKa = 11.84 YY152 pKa = 7.4 VTTEE156 pKa = 3.67 TYY158 pKa = 10.61 SRR160 pKa = 11.84 QQ161 pKa = 3.32
Molecular weight: 18.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.77
IPC_protein 3.745
Toseland 3.541
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.668
Nozaki 3.846
DTASelect 4.05
Thurlkill 3.592
EMBOSS 3.668
Sillero 3.859
Patrickios 0.947
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.779
Protein with the highest isoelectric point:
>tr|A0A5Q0JSH4|A0A5Q0JSH4_9FLAO RNA polymerase sigma factor OS=Oceanihabitans sp. IOP_32 OX=2529032 GN=FEZ18_02470 PE=3 SV=1
MM1 pKa = 8.0 PKK3 pKa = 9.06 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.44 RR11 pKa = 11.84 KK12 pKa = 9.34 RR13 pKa = 11.84 VNKK16 pKa = 9.85 HH17 pKa = 4.11 GFRR20 pKa = 11.84 EE21 pKa = 4.21 RR22 pKa = 11.84 MASANGRR29 pKa = 11.84 KK30 pKa = 9.04 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.09 GRR40 pKa = 11.84 KK41 pKa = 7.97 KK42 pKa = 10.59 LSVSSEE48 pKa = 3.61 IRR50 pKa = 11.84 HH51 pKa = 5.7 KK52 pKa = 10.81 KK53 pKa = 9.07
Molecular weight: 6.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.281
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.003
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.051
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2767
0
2767
954352
38
4615
344.9
38.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.606 ± 0.041
0.736 ± 0.016
5.487 ± 0.04
6.371 ± 0.047
5.218 ± 0.038
6.168 ± 0.053
1.905 ± 0.022
8.034 ± 0.041
8.095 ± 0.064
9.459 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.103 ± 0.026
6.426 ± 0.052
3.307 ± 0.025
3.361 ± 0.025
3.391 ± 0.032
6.275 ± 0.038
5.915 ± 0.065
6.183 ± 0.037
0.98 ± 0.018
3.979 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here