Cottoperca gobio (Frogmouth) (Aphritis gobio)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei; Eurypterygia; Ctenosquamata;

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32862 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J2PBQ2|A0A6J2PBQ2_COTGO src substrate cortactin isoform X3 OS=Cottoperca gobio OX=56716 GN=cttn PE=4 SV=1
SS1 pKa = 6.52QDD3 pKa = 3.59CVDD6 pKa = 3.83IDD8 pKa = 3.94EE9 pKa = 5.6CVDD12 pKa = 4.14LPDD15 pKa = 4.03ACVSHH20 pKa = 6.05SVCINTVGSYY30 pKa = 10.42KK31 pKa = 10.7CGGCKK36 pKa = 9.77PGFLGNQTSGCLPRR50 pKa = 11.84KK51 pKa = 9.62SCAALTFNPCDD62 pKa = 3.71INAHH66 pKa = 5.22CTMEE70 pKa = 4.09RR71 pKa = 11.84SGEE74 pKa = 4.19VACRR78 pKa = 11.84CNVGWAGNGNTCGTDD93 pKa = 3.18TDD95 pKa = 3.49IDD97 pKa = 4.4GYY99 pKa = 9.25PNRR102 pKa = 11.84SLPCMDD108 pKa = 4.32NDD110 pKa = 3.55KK111 pKa = 10.97HH112 pKa = 6.0GQTGQLCVHH121 pKa = 6.63PHH123 pKa = 6.18SGQEE127 pKa = 3.99DD128 pKa = 4.05ADD130 pKa = 3.85GDD132 pKa = 4.58GIGDD136 pKa = 3.76QCDD139 pKa = 3.33EE140 pKa = 4.81DD141 pKa = 5.57ADD143 pKa = 4.08GDD145 pKa = 4.34GIKK148 pKa = 10.51NVEE151 pKa = 4.35DD152 pKa = 3.49NCRR155 pKa = 11.84LVPNKK160 pKa = 10.07DD161 pKa = 3.51QQNSDD166 pKa = 3.18SDD168 pKa = 4.33SFGDD172 pKa = 4.74ACDD175 pKa = 3.62NCPNVPNADD184 pKa = 3.72QKK186 pKa = 11.15DD187 pKa = 3.81TDD189 pKa = 4.29SNGQGDD195 pKa = 4.7ACDD198 pKa = 3.56QDD200 pKa = 3.22IDD202 pKa = 4.35GDD204 pKa = 4.4GIPNVLDD211 pKa = 3.65NCPKK215 pKa = 10.25VPNPMQTDD223 pKa = 3.39RR224 pKa = 11.84DD225 pKa = 3.68RR226 pKa = 11.84DD227 pKa = 4.03GVGDD231 pKa = 4.08ACDD234 pKa = 3.57SCPEE238 pKa = 4.52LSNPMQTDD246 pKa = 2.72IDD248 pKa = 3.96NDD250 pKa = 4.08LVGDD254 pKa = 3.98VCDD257 pKa = 4.47TNQDD261 pKa = 3.05MDD263 pKa = 4.19GDD265 pKa = 4.35GLQDD269 pKa = 4.49SRR271 pKa = 11.84DD272 pKa = 3.79NCPDD276 pKa = 3.19IPNSSQLDD284 pKa = 3.58SDD286 pKa = 4.01NDD288 pKa = 4.11GLGDD292 pKa = 5.08DD293 pKa = 5.35CDD295 pKa = 5.44HH296 pKa = 7.79DD297 pKa = 4.92DD298 pKa = 5.57DD299 pKa = 5.8NDD301 pKa = 4.14GVLDD305 pKa = 5.21DD306 pKa = 4.8YY307 pKa = 11.88DD308 pKa = 3.61NCRR311 pKa = 11.84LVVNPNQKK319 pKa = 10.58DD320 pKa = 3.17SDD322 pKa = 4.05MNGVGDD328 pKa = 3.64VCEE331 pKa = 4.3NDD333 pKa = 3.42FDD335 pKa = 4.38NDD337 pKa = 3.56AVMDD341 pKa = 4.47LVDD344 pKa = 4.57ACPEE348 pKa = 4.08SAEE351 pKa = 4.1VTLTDD356 pKa = 3.82FRR358 pKa = 11.84AYY360 pKa = 8.36QTVILDD366 pKa = 3.91PEE368 pKa = 5.2GDD370 pKa = 3.72AQIDD374 pKa = 4.0PTWVVLNQGMEE385 pKa = 4.11IVQTMNSDD393 pKa = 3.19PGLAVGYY400 pKa = 7.31TAFNGVDD407 pKa = 3.99FEE409 pKa = 4.97GTFHH413 pKa = 6.81VNTVTDD419 pKa = 3.57DD420 pKa = 4.08DD421 pKa = 4.35YY422 pKa = 12.0AGFIFGYY429 pKa = 9.58QDD431 pKa = 2.83SSSFYY436 pKa = 10.47VVMWKK441 pKa = 7.78QTEE444 pKa = 3.81QTYY447 pKa = 8.19WQSLPFRR454 pKa = 11.84AAAQPALQLKK464 pKa = 9.2AVKK467 pKa = 10.3SRR469 pKa = 11.84TGPGEE474 pKa = 3.87FLRR477 pKa = 11.84NALWHH482 pKa = 6.33TGDD485 pKa = 3.39TPGEE489 pKa = 4.12VKK491 pKa = 10.69LLWKK495 pKa = 10.41DD496 pKa = 3.35PRR498 pKa = 11.84NVGWKK503 pKa = 10.34DD504 pKa = 3.01KK505 pKa = 10.86TSYY508 pKa = 10.19RR509 pKa = 11.84WQLSHH514 pKa = 7.03RR515 pKa = 11.84PQVGYY520 pKa = 9.37MRR522 pKa = 11.84VKK524 pKa = 10.64LFEE527 pKa = 4.36GTDD530 pKa = 3.6LVADD534 pKa = 3.84SGVVIDD540 pKa = 3.46TTMRR544 pKa = 11.84GGRR547 pKa = 11.84LGVFCFSQEE556 pKa = 3.94NIIWSNLRR564 pKa = 11.84YY565 pKa = 9.76RR566 pKa = 11.84CNDD569 pKa = 3.41TLPEE573 pKa = 4.29DD574 pKa = 4.26FQTHH578 pKa = 5.09RR579 pKa = 11.84QQFLMHH585 pKa = 6.27IRR587 pKa = 11.84VV588 pKa = 3.36

Molecular weight: 64.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J2QMP6|A0A6J2QMP6_COTGO protein FAM131B-like isoform X1 OS=Cottoperca gobio OX=56716 GN=LOC115015785 PE=3 SV=1
MM1 pKa = 7.12MMKK4 pKa = 9.6ICVLLLLCVASGNTQITVAPTTAAPAAATTVAPTTAAPAAATTVAPTTAAPAAATTAAPPATTAAPAAITAEE76 pKa = 4.18ATTAAPAAITAEE88 pKa = 4.18ATTAAPAAITAEE100 pKa = 4.19ATTVAPAAITAATAAATTAAAPAAITAATAAATTAAAPAATTAAATVAPAATATAAAATTVAPTTAAPAQQPQLPQPQRR179 pKa = 11.84PRR181 pKa = 11.84QQQPRR186 pKa = 11.84RR187 pKa = 11.84PRR189 pKa = 11.84LQLQRR194 pKa = 11.84TQPRR198 pKa = 11.84RR199 pKa = 11.84PRR201 pKa = 11.84QQLQRR206 pKa = 11.84KK207 pKa = 5.87QPRR210 pKa = 11.84RR211 pKa = 11.84PRR213 pKa = 11.84QQLQRR218 pKa = 11.84KK219 pKa = 5.87QPRR222 pKa = 11.84RR223 pKa = 11.84PRR225 pKa = 11.84QQLQRR230 pKa = 11.84KK231 pKa = 5.87QPRR234 pKa = 11.84RR235 pKa = 11.84PRR237 pKa = 11.84QQLQRR242 pKa = 11.84KK243 pKa = 5.01QPRR246 pKa = 11.84RR247 pKa = 11.84RR248 pKa = 11.84RR249 pKa = 11.84QQLQRR254 pKa = 11.84KK255 pKa = 5.88QPRR258 pKa = 11.84RR259 pKa = 11.84PRR261 pKa = 11.84QQLQRR266 pKa = 11.84KK267 pKa = 7.41QPQRR271 pKa = 11.84PRR273 pKa = 11.84QQPRR277 pKa = 11.84RR278 pKa = 11.84KK279 pKa = 8.59PRR281 pKa = 11.84RR282 pKa = 11.84PRR284 pKa = 11.84HH285 pKa = 5.06HH286 pKa = 6.55QQLQLPPLQSSTKK299 pKa = 9.62QVSFF303 pKa = 4.06

Molecular weight: 32.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21119

11743

32862

22561719

31

30276

686.6

76.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.61 ± 0.011

2.109 ± 0.01

5.273 ± 0.008

7.038 ± 0.017

3.339 ± 0.01

6.373 ± 0.02

2.663 ± 0.009

4.236 ± 0.013

5.673 ± 0.019

9.237 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.397 ± 0.006

3.797 ± 0.009

5.943 ± 0.02

4.904 ± 0.022

5.68 ± 0.011

8.98 ± 0.02

5.759 ± 0.015

6.26 ± 0.02

1.087 ± 0.005

2.64 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski