Canary polyomavirus
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E0XL35|E0XL35_9POLY Small t antigen OS=Canary polyomavirus OX=881945 PE=4 SV=1
MM1 pKa = 8.05 DD2 pKa = 5.66 PGSYY6 pKa = 10.99 AEE8 pKa = 5.24 LLSLLQLPRR17 pKa = 11.84 NADD20 pKa = 3.32 EE21 pKa = 4.66 KK22 pKa = 11.04 DD23 pKa = 2.75 ISRR26 pKa = 11.84 AYY28 pKa = 10.27 RR29 pKa = 11.84 KK30 pKa = 9.5 VALQMHH36 pKa = 7.18 PDD38 pKa = 3.21 KK39 pKa = 11.35 GGNADD44 pKa = 3.34 KK45 pKa = 10.45 MKK47 pKa = 10.58 RR48 pKa = 11.84 LNALMEE54 pKa = 4.25 QYY56 pKa = 10.13 RR57 pKa = 11.84 SSGGSSGPDD66 pKa = 3.23 LSCNEE71 pKa = 4.64 SPLQSEE77 pKa = 4.99 DD78 pKa = 3.68 EE79 pKa = 4.49 DD80 pKa = 4.48 PEE82 pKa = 4.34 EE83 pKa = 4.79 GPSGEE88 pKa = 4.49 SAGSAGYY95 pKa = 9.89 FSQSMPRR102 pKa = 11.84 SPPVSSQEE110 pKa = 4.01 YY111 pKa = 8.21 ISAYY115 pKa = 9.39 MKK117 pKa = 10.55 LLKK120 pKa = 10.49 FKK122 pKa = 10.46 MSMDD126 pKa = 2.86 AFMRR130 pKa = 11.84 HH131 pKa = 5.28 CEE133 pKa = 3.91 RR134 pKa = 11.84 RR135 pKa = 11.84 KK136 pKa = 9.69 QCLTPEE142 pKa = 4.84 FGRR145 pKa = 11.84 MAEE148 pKa = 4.11 NYY150 pKa = 9.56 YY151 pKa = 10.42 AIPWGVFGRR160 pKa = 11.84 LFKK163 pKa = 10.52 EE164 pKa = 4.01 TDD166 pKa = 2.99 LL167 pKa = 5.19
Molecular weight: 18.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.295
IPC2_protein 5.474
IPC_protein 5.359
Toseland 5.359
ProMoST 5.525
Dawson 5.385
Bjellqvist 5.486
Wikipedia 5.258
Rodwell 5.308
Grimsley 5.296
Solomon 5.385
Lehninger 5.347
Nozaki 5.525
DTASelect 5.652
Thurlkill 5.397
EMBOSS 5.334
Sillero 5.601
Patrickios 4.101
IPC_peptide 5.397
IPC2_peptide 5.601
IPC2.peptide.svr19 5.679
Protein with the highest isoelectric point:
>tr|E0XL32|E0XL32_9POLY Minor capsid protein VP2 OS=Canary polyomavirus OX=881945 PE=3 SV=1
MM1 pKa = 7.2 GAIVSIFVAIGEE13 pKa = 4.34 AVAATGLAAEE23 pKa = 4.86 TIISGEE29 pKa = 3.68 AAAILGAQISSLAAEE44 pKa = 5.01 GFTTAEE50 pKa = 4.13 ALSTLGLTPEE60 pKa = 4.75 LFAFTTSLASAAEE73 pKa = 4.18 SGGALWTGIASGLYY87 pKa = 8.9 SLGGAGVSGIAGGLQGGIATAAAGLVRR114 pKa = 11.84 FPNISKK120 pKa = 9.54 QRR122 pKa = 11.84 HH123 pKa = 4.21 EE124 pKa = 4.34 MALQIWQPLDD134 pKa = 3.0 WLYY137 pKa = 10.69 PEE139 pKa = 6.0 AEE141 pKa = 3.95 WLYY144 pKa = 10.94 NAYY147 pKa = 10.24 HH148 pKa = 6.2 YY149 pKa = 10.09 FDD151 pKa = 5.15 PYY153 pKa = 11.09 SWLPSLIDD161 pKa = 3.22 QLGYY165 pKa = 10.18 YY166 pKa = 9.11 FWEE169 pKa = 4.34 YY170 pKa = 11.11 VEE172 pKa = 4.69 RR173 pKa = 11.84 TGQRR177 pKa = 11.84 QLASQATAVAVRR189 pKa = 11.84 ATNRR193 pKa = 11.84 AWYY196 pKa = 8.36 TLTEE200 pKa = 4.24 QISNAGWFIRR210 pKa = 11.84 NGYY213 pKa = 8.4 YY214 pKa = 10.69 ALEE217 pKa = 4.27 HH218 pKa = 6.54 YY219 pKa = 10.59 YY220 pKa = 10.45 RR221 pKa = 11.84 VLPPRR226 pKa = 11.84 FGPPRR231 pKa = 11.84 LRR233 pKa = 11.84 SYY235 pKa = 11.51 LEE237 pKa = 3.8 ANRR240 pKa = 11.84 EE241 pKa = 4.01 NVFIDD246 pKa = 4.09 PPDD249 pKa = 3.83 MGSEE253 pKa = 3.94 TSDD256 pKa = 2.94 NRR258 pKa = 11.84 FEE260 pKa = 5.2 GDD262 pKa = 3.08 FLKK265 pKa = 10.64 RR266 pKa = 11.84 LKK268 pKa = 10.67 KK269 pKa = 9.93 IFSRR273 pKa = 11.84 EE274 pKa = 3.86 QEE276 pKa = 4.04 SGEE279 pKa = 3.96 RR280 pKa = 11.84 VYY282 pKa = 11.05 KK283 pKa = 10.47 SRR285 pKa = 11.84 PPGGAEE291 pKa = 4.51 QMVTPDD297 pKa = 3.11 WLLPLILGLLGDD309 pKa = 4.49 LTPTFAADD317 pKa = 2.8 IRR319 pKa = 11.84 RR320 pKa = 11.84 YY321 pKa = 10.08 GSEE324 pKa = 3.43 NRR326 pKa = 11.84 KK327 pKa = 9.58 RR328 pKa = 11.84 KK329 pKa = 8.4 ATSTSTPPVNKK340 pKa = 9.51 RR341 pKa = 11.84 RR342 pKa = 11.84 NRR344 pKa = 11.84 GTRR347 pKa = 11.84 PKK349 pKa = 10.68 DD350 pKa = 3.23 RR351 pKa = 11.84 RR352 pKa = 11.84 RR353 pKa = 11.84 LNNNSRR359 pKa = 11.84 GLSKK363 pKa = 10.65 SQNGNRR369 pKa = 3.8
Molecular weight: 40.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.776
IPC2_protein 8.96
IPC_protein 9.077
Toseland 9.194
ProMoST 9.282
Dawson 9.575
Bjellqvist 9.355
Wikipedia 9.838
Rodwell 9.619
Grimsley 9.706
Solomon 9.619
Lehninger 9.56
Nozaki 9.151
DTASelect 9.355
Thurlkill 9.355
EMBOSS 9.648
Sillero 9.472
Patrickios 4.533
IPC_peptide 9.604
IPC2_peptide 8.039
IPC2.peptide.svr19 7.905
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1762
167
625
352.4
39.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.435 ± 1.2
1.589 ± 0.689
4.824 ± 0.534
6.583 ± 0.546
3.292 ± 0.231
8.116 ± 1.241
1.532 ± 0.351
3.462 ± 0.478
4.824 ± 0.801
10.556 ± 0.546
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.27 ± 0.458
5.051 ± 0.368
6.47 ± 0.351
3.462 ± 0.187
6.924 ± 0.949
6.924 ± 0.765
6.073 ± 0.878
5.165 ± 1.091
1.249 ± 0.523
4.2 ± 0.46
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here