Cercospora berteroae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Cercospora

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11903 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S6BSI7|A0A2S6BSI7_9PEZI Ribokinase OS=Cercospora berteroae OX=357750 GN=CBER1_05560 PE=3 SV=1
MM1 pKa = 7.6RR2 pKa = 11.84SAVRR6 pKa = 11.84GNFWYY11 pKa = 7.13TTSVQQTVATVISTVLDD28 pKa = 3.53YY29 pKa = 11.52GDD31 pKa = 3.85RR32 pKa = 11.84EE33 pKa = 4.25EE34 pKa = 4.46VGNVSTNYY42 pKa = 9.78VPADD46 pKa = 3.26QLYY49 pKa = 8.57TSFGVYY55 pKa = 10.34RR56 pKa = 11.84IRR58 pKa = 11.84TVIFGDD64 pKa = 3.88DD65 pKa = 3.24VVTVNRR71 pKa = 11.84EE72 pKa = 3.88LEE74 pKa = 4.17ALGVPSTLIQGRR86 pKa = 11.84DD87 pKa = 3.32YY88 pKa = 11.46AATIVYY94 pKa = 8.69QANASFDD101 pKa = 3.52HH102 pKa = 6.23GVAYY106 pKa = 7.38PTPFAIWNDD115 pKa = 3.6VQVLYY120 pKa = 11.0LDD122 pKa = 3.83QTTSCIPTATGRR134 pKa = 11.84PTISRR139 pKa = 11.84LEE141 pKa = 4.13GYY143 pKa = 10.34GRR145 pKa = 11.84IDD147 pKa = 3.27IDD149 pKa = 3.19RR150 pKa = 11.84HH151 pKa = 5.02EE152 pKa = 5.11DD153 pKa = 3.09GRR155 pKa = 11.84YY156 pKa = 9.87LSDD159 pKa = 3.38RR160 pKa = 11.84ATKK163 pKa = 10.38YY164 pKa = 10.62YY165 pKa = 7.51YY166 pKa = 10.13TPSAYY171 pKa = 10.15DD172 pKa = 3.27SEE174 pKa = 5.38RR175 pKa = 11.84YY176 pKa = 9.53FYY178 pKa = 11.31VQTLPLDD185 pKa = 4.22GPQPSLGNYY194 pKa = 8.25ALPSGAIEE202 pKa = 4.57RR203 pKa = 11.84YY204 pKa = 10.14VSIQSSAYY212 pKa = 9.42PWITDD217 pKa = 3.61CTPADD222 pKa = 3.94TPGEE226 pKa = 4.25PTVHH230 pKa = 6.43IAVNQLTDD238 pKa = 3.32TSRR241 pKa = 11.84VTVRR245 pKa = 11.84MAGPTTDD252 pKa = 3.4PTPRR256 pKa = 11.84PTGDD260 pKa = 3.15TPALPGTPSPAPPSEE275 pKa = 5.36DD276 pKa = 3.37DD277 pKa = 4.56DD278 pKa = 4.57EE279 pKa = 5.62PIPVQTPTPPTEE291 pKa = 5.4DD292 pKa = 3.59DD293 pKa = 4.39DD294 pKa = 5.9GPVPVQPPANTQVPNSGDD312 pKa = 3.63TPNTGSDD319 pKa = 3.39GSPPQNPTTGEE330 pKa = 3.95QQPANPGEE338 pKa = 4.11TRR340 pKa = 11.84PDD342 pKa = 3.05NSSPNNAGPGGTSFEE357 pKa = 4.27DD358 pKa = 3.74TSPNTQQSPASSNPQSPNPNQNDD381 pKa = 3.37QGPQSDD387 pKa = 4.86DD388 pKa = 3.53SDD390 pKa = 5.69DD391 pKa = 4.16IPSSPAVVVPGEE403 pKa = 4.25LPASGTVQGGAQGQTTAPGSGDD425 pKa = 3.57DD426 pKa = 4.05TSDD429 pKa = 3.22EE430 pKa = 4.42SVPSGTGTDD439 pKa = 3.92DD440 pKa = 3.36QTSGATGSDD449 pKa = 4.04DD450 pKa = 3.75EE451 pKa = 5.75VEE453 pKa = 4.49DD454 pKa = 4.73APASGDD460 pKa = 3.4SSATGDD466 pKa = 3.46VGDD469 pKa = 5.63AIMSGIGQTRR479 pKa = 11.84TGSNGAGYY487 pKa = 8.08TGPAYY492 pKa = 9.5TGKK495 pKa = 10.68APALMANFVVVASMGLTWLAWGCMM519 pKa = 3.77

Molecular weight: 54.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S6BSK1|A0A2S6BSK1_9PEZI Uncharacterized protein OS=Cercospora berteroae OX=357750 GN=CBER1_05571 PE=4 SV=1
MM1 pKa = 7.35NGLGPFMSIGRR12 pKa = 11.84GRR14 pKa = 11.84ITMFSPGGRR23 pKa = 11.84GRR25 pKa = 11.84GTQIMTIGGNGFINPAGSSIQIFGNGRR52 pKa = 11.84GGMNIMSNGGRR63 pKa = 11.84GRR65 pKa = 11.84GNYY68 pKa = 10.29SGMQSGTGFNNLGQIQQITMPNPMTGAGGGMFMSMPGMTMSIPFQGGRR116 pKa = 11.84GTGRR120 pKa = 11.84GTGFGLTSAGSSASGSRR137 pKa = 11.84ASGSSRR143 pKa = 11.84SSSSRR148 pKa = 11.84APGSTASGSMGRR160 pKa = 11.84GSSGRR165 pKa = 11.84GSHH168 pKa = 6.58ASSAAGAGSVYY179 pKa = 10.5GGSQGPFGGGGGGTAAPP196 pKa = 4.29

Molecular weight: 18.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11903

0

11903

5823408

66

7529

489.2

54.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.195 ± 0.02

1.169 ± 0.008

5.851 ± 0.015

6.358 ± 0.021

3.568 ± 0.014

6.92 ± 0.022

2.422 ± 0.009

4.647 ± 0.014

4.952 ± 0.021

8.585 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.158 ± 0.009

3.701 ± 0.014

5.902 ± 0.025

4.327 ± 0.017

6.097 ± 0.02

7.882 ± 0.025

6.047 ± 0.02

5.997 ± 0.015

1.468 ± 0.009

2.753 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski