Pseudorhodobacter turbinis
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3753 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8EL10|A0A4P8EL10_9RHOB Uncharacterized protein OS=Pseudorhodobacter turbinis OX=2500533 GN=EOK75_17275 PE=4 SV=1
MM1 pKa = 8.16 RR2 pKa = 11.84 DD3 pKa = 3.07 RR4 pKa = 11.84 STPAMTLQPLAMALCFMVSAATTGSAATIGQPLVIAEE41 pKa = 4.33 ADD43 pKa = 3.53 GTGYY47 pKa = 10.51 ILDD50 pKa = 4.26 YY51 pKa = 10.86 FVGLYY56 pKa = 10.14 PSGLKK61 pKa = 10.6 AIDD64 pKa = 3.64 ASATVTEE71 pKa = 4.26 EE72 pKa = 3.72 HH73 pKa = 6.96 DD74 pKa = 3.76 TNGFLGSTVDD84 pKa = 3.33 YY85 pKa = 11.19 TMNLDD90 pKa = 3.47 GNGTFRR96 pKa = 11.84 LGAYY100 pKa = 9.12 AFNIITFTDD109 pKa = 4.22 GFAGAWGPDD118 pKa = 3.3 FKK120 pKa = 11.59 DD121 pKa = 3.75 FDD123 pKa = 4.21 DD124 pKa = 4.28 TGLITTTFLVEE135 pKa = 4.32 FPILSTCVDD144 pKa = 4.03 PDD146 pKa = 3.86 SGSDD150 pKa = 3.91 LYY152 pKa = 11.05 TIDD155 pKa = 5.18 LDD157 pKa = 3.99 PFGVGLAIEE166 pKa = 4.51 VLDD169 pKa = 3.69 ATYY172 pKa = 11.35 AEE174 pKa = 4.02 IGVNISYY181 pKa = 10.99 DD182 pKa = 3.43 EE183 pKa = 4.76 DD184 pKa = 4.36 LPCKK188 pKa = 10.45 LLVDD192 pKa = 4.33 DD193 pKa = 6.12 RR194 pKa = 11.84 SDD196 pKa = 2.95 EE197 pKa = 4.14 YY198 pKa = 11.76 YY199 pKa = 10.75 YY200 pKa = 10.21 IEE202 pKa = 4.91 GFFTTTRR209 pKa = 11.84 IEE211 pKa = 3.85 VGLIGGAPVAAVPLPAGGLLLLTGLVGLFGWKK243 pKa = 9.36 KK244 pKa = 10.27 RR245 pKa = 11.84 RR246 pKa = 11.84 TT247 pKa = 3.68
Molecular weight: 26.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.695
IPC2_protein 3.821
IPC_protein 3.834
Toseland 3.605
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.783
Rodwell 3.656
Grimsley 3.516
Solomon 3.821
Lehninger 3.783
Nozaki 3.948
DTASelect 4.215
Thurlkill 3.668
EMBOSS 3.795
Sillero 3.961
Patrickios 1.138
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.833
Protein with the highest isoelectric point:
>tr|A0A4P8EEU0|A0A4P8EEU0_9RHOB Copper chaperone PCu(A)C OS=Pseudorhodobacter turbinis OX=2500533 GN=EOK75_07435 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 TRR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.68 GFRR19 pKa = 11.84 LRR21 pKa = 11.84 MSTKK25 pKa = 10.13 AGRR28 pKa = 11.84 NILNLRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.16 GRR39 pKa = 11.84 KK40 pKa = 8.85 KK41 pKa = 10.63 LSAA44 pKa = 3.95
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3753
0
3753
1189126
30
5560
316.8
34.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.414 ± 0.059
0.904 ± 0.014
5.862 ± 0.035
5.477 ± 0.04
3.754 ± 0.028
8.598 ± 0.045
2.043 ± 0.022
5.42 ± 0.031
3.467 ± 0.031
10.12 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.912 ± 0.024
2.747 ± 0.028
4.965 ± 0.033
3.289 ± 0.022
6.356 ± 0.039
5.294 ± 0.036
5.641 ± 0.038
7.193 ± 0.035
1.334 ± 0.018
2.209 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here